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ThesisItem Open Access Detection of Autoinducer-II (AI-2) Regulatory Genes in Multi-Drug Resistance (MDR) Zoonotic Bacterial Pathogens and their inhibitory phytochemicals(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021-10) MRINAL SRIVASTAVA; Dr. Amit KumarAbstract Name: Mrinal Srivastava I.D No: 4891 Degree: M.Tech (biotechnology) Division: Animal Biotechnology College of Biotechnology Thesis Title: Detection of Autoinducer-II (AI-2) regulatory genes in Multi-drug Resistance (MDR) zoonotic bacterial pathogens and their inhibitory phytochemicals. The worldwide utilization and abuse of antibiotics has led to the progression and outspread of bacterial resistance against all regularly used antibacterials. In order to successfully tackle broaden of antimicrobial resistance (AMR) through development of novel antimicrobial compounds or antimicrobial stewardship, interpretating how bacteria transform and develop to live on antibiotic treatments is critical. Keeping these aspects in mind present study was designed to isolate and identify multi drug resistance (MDR) zoonotic bacterial pathogens mainly focusing on E. coli and pseudomonas. In total of 100 samples received in two groups i.e. 50 samples from milk and 50 from faecal. The study revealed 23 isolates based on cultural, morphological, biochemical and molecular characterization. Out of 50 milk samples 9 isolate was confirmed as E.coli and 6 as Pseudomonas and in another 50 samples of faecal, 4 isolates was confirmed as E.coli and 4 as Pseudomonas. All the isolates of E. coli were confirmed by the amplification species specific of 556 base pair uidA genes and isolates of pseudomonas were confirmed by PseudF/R- 16Sr RNA primers of 618 base pair. drug resistance was performed by phenotypic and molecular screening of multi drug resistance isolates. All the bacterial isolates were subjected to antibiotic sensitivity test against 9 antibacterial drugs like: Amoxyclav, Amikacin, Cefoperazone, Cefalexin, Celtriaxene, Levofloxacin, Gentamicin, Streptomycin and PenicillinG. PenicillinG was the only drug against which 100% resistance was recorded by all the isolates irrespective of species of isolates and also the origin of isolates. When all the isolates were classified for antibiotyping, they revealed 13 biotypes of E. coli and 5 biotypes in Pseudomonas. For molecular screening of the multi drug resistance isolates two different group of antimicrobial resistance were selected for the screening i.e. ESBL resistance and carbapenem resistance. All E. coli isolates were subjected for the amplification of two different resistant gene i.e. blaSHV and blaNDM. Amplicons were subjected to Sanger sequencing and sequences of all these genes were submitted in NCBI database to obtain the accession number. Out of 13 E. coli isolates 4 each confirmed the presence of blaSHV and blaNDM genes. Discovery of microbial quorum sensing (QS) has administered new hope for studying the regulatory mechanism of drug resistance and overcoming drug resistance QS system. Numerous bacteria can communicate by contributing and responding to a small diffusible molecule that act as signals. These molecules can be described as auto inducers (AIs). Keeping these aspects study was designed for its second objective for the designing of Autoinducer-II (AI-2) gene primers for isolated major zoonotic bacterial pathogens. These primers were used to fullfil third objective of molecular detection of AI-2 gene in multi drug resistance (MDR) in zoonotic bacterial pathogens. Out of 13 confirmed isolates 12 isolates of E. coli revealed an amplification product of 290bp and accordingly confirmed to be mqsr- AI-2. Whereas. out of 10 confirmed isolates only 6 isolates of the pseudomonas revealed an amplification product of 345bp, resulting in the confirmation of rhIR AI-2 gene. Amplicons were subjected to Sanger sequencing and received data was analyzed. The forth objective was in silico docking based assessment of phytochemicals for inhibition of quorum sensing AI-2 gene. The ten phytoconstituents of Ocimum sanctum showed different positive docking scores against autoinducer-2 translational protein of E. coli and Pseudomonas aeruginosa. However, Germacrene and Rosmarinic acid suggested good docking scores for autoinducer-2 translational protein of Pseudomonas aeruginosa. Out of these Rosmarinic acid full fill all the basec requirements to be a potential drug moleculae. (Mrinal Srivastava) (AmitKumar) I.D No: 4891 AdvisorThesisItem Open Access Studies on Characterization of Microorganisms for Solubilization of Phosphate, Potassium and Zinc(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2020) Mansi Tyagi; Akash TomarABSTRACT Name: Mansi Tyagi Id. No. : 4236 Semester and year of admission: 1st sem. 2018-2019 Degree: M. Tech. Major: Biotechnology Department: Biotechnology Thesis Title: “Studies on Characterization of Microorganisms for Solubilization of Phosphate, Potassium and Zinc.” Advisor: Dr. Akash Tomar Soil is the basis of life and we living beings are directly and indirectly depend on it. Agriculture is based fully on the soil because without it agriculture cannot sustain, it is the building block of agriculture. Soil have several organic, inorganic materials in it. Nutrients are one of the important part of soil because plants are directly influenced by the availability of nutrients. Deficiency of these nutrients causes severe damage to growth and yield of plants and crops. These are the basic need of plants and major nutrients include Phosphorous, Nitrogen, and Potassium. Rhizospheric soil samples were collected from native crop fields and nearby regions. Potential microbes were isolated and tested for solubilization of P, K and Zn on Pikovskayas medium, Aleksandrov’s agar medium and on Zinc solubilizing agar medium that were supplemented with P, K and Zn source. Mineral solubilization involves releasing of these bound nutrients by soil microorganism and making them available for plant uptake via three possible mechanisms that are acidification of the medium, production of chelating metabolites, and redox activity.The nutrient solubilizing bacteria generates a halo zone around their colony on the basis of which their solubilization potential was identified.A total of 18 isolates, two isolated from soil and 16 from previous studies, were tested for multiple solubilization potential, some were found to solubilize all the three nutrients.Microscopic studies showed presence of bacteria with rods, oval, and cocci cell morphology. 17 isolates were gram negative and one Gram positive. Colony morphology showed05 isolates were greenish-yellow fluorescence producing bacteria denoted as MS-1, PYS-2, PYS-3, PYS-11, PYS-14 and S6 producing yellowish green fluorescence,02 isolates S4 and MS-3 were brown pigment producing gram negative rods.IsolateSS-9-2 was gram positive with cocci or in some instance streptococci cell arrangement. During plate assay for solubilization,isolates S4, S6, MS-1, PYS-14 and SS-9-2 showed highest level of solubilization of P present in the form of tricalcium phosphate. Isolates S1, S6, MS-1, MS-3, SS-7-6, SS-7-7and SS-9-2 showed potential to solubilize K present in the form of potassium aluminosilicate. Whereas Zn present as zinc oxide in media was solubilized by S1, S6, MS-3, PYS-2, PYS-11, PYS-14, SS 7-6 and SS-9-2. The study showed that isolates S1, S6, MS-1, PYS-2, PYS-3, PYS-11, PYS-14 and SS-9-2 can solubilize either of the nutrient (P, K and Zn), an, two or all the three efficiently.On the basis of above study 09 potential isolates (S1, S4, S6, MS-1, MS-3, PYS-2, SS-7-6, SS-7-7 and SS-9-2) were selected to perform biochemical tests for their diversity analysis. 13 biochemical tests were performed to identify isolates. Isolates showed different characteristics in terms of morphology, solubilization assay and biochemical tests but some on the other hand showed similarities in either of the aspect making them relating to same genera. The results indicated that isolates differ in their ability and potential to solubilize multiple nutrients, it was also imperative that many isolates were showing higher solubilization of one specific nutrient only, some of the isolates were poor solubilizers of two or in some instance all three nutrients. These isolates however may serve as reference stocks for further studies on nutrient solubilization assay and its mechanisms. (Akash Tomar) (Mansi Tyagi) ADVISOR AUTHORThesisItem Open Access Assessment of molecular markers to detect the DNA damage caused by Parthenium plant extract(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2020-10) AKANKSHA SINGH; Dr. Shalani GuptaABSTRACT Name : Akanksha Singh Id. No : 2874 Year of admission : 2018 Degree : M.Tech (Biotechnology) College : College of Biotechnology Thesis title : Assessment of molecular markers to detect the DNA damage caused by Parthenium plant extract The aim of the present study was to evaluate the phytotoxicity, cytotoxicity and DNA damage caused by Parthenium plant a invasive weed on selected crop plants. Three different concentrations of Parthenium plant extract i.e. 2.5 %, 5.0 % and 7.5 % were used as test material. Phytotoxic effects of Parthenium plant on seedling traits in four crop plants viz. V. faba, H. vulgare, C. arietinum and S. lycopersicum showed significant reduction in all seedling traits using three extract concentrations as compared to control. Parthenium plant extract concentration at 7.5 % has maximum negative effect on seedling traits. V. faba has least effect of Parthenium extracts treatments on seedling traits as compared to other crops whereas S. lycopersicum was highly affected by Parthenium extracts. Cytotoxic effects of Parthenium plant on mitotic index in roots of A. cepa showed reduction in mitotic activity using three extract concentrations both at 24 and 48 hours. Where relative abnormality rate (RAR) increased with increase in concentration as well as treatment duration. Several chromosomal aberrations were recorded i.e. early prophase, sticky metaphase, C- metaphase, disturbed metaphase and anaphase, forward metaphase anaphase and telophase chromosomes, laggards in anaphase and telophase, bridges at anaphase and telophase, micronucleus at inter-phase. SSR markers were assayed to assess the DNA damage caused by Parthenium plant extract in V. faba and H. vulgare with 15, 30 and 45 days treated samples revealed some newly induced bands and some normal bands disappeared which shows change in genomic DNA. In V. faba GTS value varies from 42.90 % (7.5 % of 15 days treated sample) to 75 % (5.0 % of 30 days treated sample). In H. vulgare GTS values varies from 16.67 % (5.0 % and 7.5 % of 30 and 45 days treated samples) to 83.33 % (2.5 % of 15 and 45 days treated). Marker analysis shows that Parthenium plant extract was capable of inducing DNA damage as lower estimated GTS values reflects high to medium genetic variability, although its suggested more studies on this aspect to save field crops from damage on DNA. (Shalani Gupta) (Akanksha Singh) Name and Sign of Advisor Name and Sign of AuthorThesisItem Open Access “Identification and Molecular Characterization of Potyvirus Infecting garlic (Allium sativum L.)”(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021-11) RAHUL; Dr. Jitender SinghAbstract Name : Rahul Id No. : 3305 Degree : M. Tech (Biotechnology) Department : Immunology and Defence Mechanism Thesis title : “Identification and molecular characterization of potyvirus infecting garlic (Allium sativum)” Garlic, (Allium sativum) is second largest cultivated species in India. It plays significant role in flavouring food and medicine. However, due to vegetative propagation it susceptible to virus infection as well as virus co-infection which causes symptoms such as yellowing, mosaic, chlorotic, yellow striping on the leaves and which reduce the quality and quantity of the garlic. Therefore, to control the viral disease molecular level identification is very important. In the present study, the presence of the pathogen was confirmed by reverse transcription-polymerase chain reaction (RT-PCR). Leek yellow stripe virus (LYSV) and Onion yellow dwarf virus (OYDV) ~860bp and ~1111 bp amplicons were obtained. Blast analysis showed that the samples were infected with LYSV and OYDV of genus Potyvirus, family Potyviridae. The BLASTn showed the sequences obtained with LYSV (OL312049 and OL312050) shared maximum sequence identity with LYSV; 96.19% garlic, India (KP168262). The BLASTn analysis of OYDV sequences (OL312046, OL312047 and OL312048) shared maximum sequence identity with OYDV; 93.84–92.88% onion, India (KP862052 and KP862053) and 84.46% garlic-China (AJ510223). Phylogenetic analysis showed a close relationship of LYSV and OYDV isolates grouped in same clade. On the basis of the above results, it can be concluded that LYSV and OYDV were present in the garlic samples. This will help in understanding the evolutionary patterns of potyviruses and is also important in developing potyvirus disease management strategies.ThesisItem Open Access MORPHO-PHYSIO-BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SELECTED POTATO (Solanum tuberosum L.) VARIETIES UNDER DROUGHT STRESS(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2020-12) VYANKATESH DHANRAJ BAGUL; Dr. R. S. SengarABSTRACT Name: Vyankatesh Dhanraj Bagul Id. No.: PG/4357/18-19 Degree: M.Sc. (Ag.) Major: Agriculture Biotechnology College: College of Agriculture Minor: Genetics and Plant Breeding Advisor: Dr. R. S. Sengar Thesis title: “Morpho-physio-biochemical and molecular characterization of selected potato (Solanum tuberosum L.) varieties under drought stress”. Potato (Solanum tuberosum L.) is considered as an important cash crop of the world and also known as apple of soil. It provides food for millions of people across the globe with being the 4th largest produced crop. Drought is one of the major stress factors affecting the growth and development of potato plants. In this study, we have selected 5 Indian potato varieties and supplemented them with 2 different drought treatments viz., allowed them to undergo heavy drought stress and application of aquasorb at the time of sowing at the rate of 30Kg/ha. Both the treatments were subjected for 15 days of drought stress by water withholding at tuber initiation stage which is known to be the most vulnerable stage due to drought stress. The experiment was conducted in CRD pattern using experimental pots at the rain out shelter of Department of Agriculture Biotechnology. Various morpho-physiobiochemical parameters regarding drought stress responses of plant were selected, measured and compared within the varieties after the drought stress period was completed. Also, seven drought related gene specific marker were synthesized using bioinformatics tools and were used for molecular characterization of selected potato varieties. The study suggests that, drought has negatively impacted on all the varieties used in the experiments regardless of their stress response nature. The impact of drought stress varied among the plant varieties, where K. Thar-1, a drought tolerant variety under drought stress was found significantly superior over K. Pukhraj, K. Bahar, K. Chipsona-1 and K. Chipsona-1 in the morpho-physio-biochemical parameters namely no. of compound leaves per plant (10.3), no. of tubers per plant (18.67), weight of tubers per plant (238.89gm), top biomass weight per plant (336.83gm), root biomass weight (20.7gm), average stolon length (10.8cm), chlorophyll content(31.80), leaf area index (1.52), relative water content (56.45%), sucrose content (381.49mg), reducing sugar content (73.87mg), proline content (28.83μmols), catalase activity (24.57U), peroxidase activity (61.45ΔA/min) and abscisic acid content (256.49pmol). In aquasorb treated drought stressed conditions, all the varieties shown great resilience and were found to be significantly superior over drought stressed conditions. This experiment suggest that Aquasorb can help to minimize the losses occurred in crop production. Molecular characterization of these selected potato varieties gave three polymorphic bands and seven monomorphic bands at an average pf 1.43 bands per primer. The dendrogram using bioinformatics tools explains inter-varietal hierarchical relationship. (R. S. Sengar) (Vyankatesh Bagul) Advisor AuthorThesisItem Open Access A study on phytochemical profiling and antimicrobial activity of Cymbopogon citratus (Lemon grass(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021-10) Saumya Jaiswal; Dr. Pankaj ChauhanABSTRACT Name: Saumya Jaiswal Id. No: 4890 Degree: M.Sc. Biotechnology Department: Department of Cell Biology Thesis title: “A study on phytochemical profiling and antimicrobial activity of Cymbopogon citratus (Lemon grass)” In recent years researchers have been paid attention to find out new alternative sources of antimicrobial agents especially from plant sources. Cymbopogon citratus (Lemon grass) is an aromatic medicinal plant in the family Poaceae. The main chemical components of lemon grass are: myrcene, geranial, limonene, citronellol, neral, caffeic acid, citral have been used extensively for many years in soap, perfumery, and detergents and pharmaceuticals. Phytochemical screening of the plant leaf reveals that the presence of saponins, alkaloids, flavonoids, terpenoids, phenols, tannins and glycosides. Different leaf extracts of Cymbopogon citratus leaves were prepared and its antimicrobial activity were evaluated by agar well diffusion method against both Bacterial species such as: Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli as well as Fungi pathogens such as: Aspergillus fumigatus, Candida albicans, Penicillium Chrysogenum. The antimicrobial activity of crude methanolic, ethanolic, acetone and aqueous extract of C. citratus was evaluated to find the zone of inhibition, so that Antimicrobial activity of various extract of leaves of Cymbopogon citratus was carried in attempt to develop a new pharmaceutical drug from natural origin for prevention of pathogenic microbes. Key words: Cymbopogon citratus, Lemon grass, Phytochemicals, Zone of Inhibition, Extract, Microbes (Saumya Jaiswal) (Pankaj Chauhan) Id.No.4890 Advisor Curriculum Vitae Saumya Jaiswal Contact Address:ThesisItem Open Access Studies on isolation and characterization of rhizosperic fungi for phosphate solubilisation activity under in vitro condition(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021) DEEKSHA CHAUDHARY; Dr.Akash TomarABSTRACT Name: DEEKSHA CHAUDHARY Id. No. : 4889 Semester and year of admission: 1 sem. 2019-2020 Degree: M. Tech/M.sc Major: Biotechnology College: Biotechnology Advisor: Dr. Akash Tomar Thesis Title: “Studies on isolation and characterization of rhizospheric fungi for phospahte solubilisation activity under invitro condition.” Plant require phosphorus, a vital plant nutrient, it is found in fixed forms in most soil. Thus, poor availability of P in soil and consequent P-deficiency represents a major constraint to crop production globally. This has led to a search for environment-friendly and economically feasible alternative strategies for improving crop production in low or P deficient soils, PSM are known to bring about mobilisation of insoluble phosphates and stimulate plant development even under the condition of phosphorus deficiency. Mineral solubilization is the mechanism of soil microorganisms releasing bound nutrients and making them available for plant uptake by one of three mechanisms: acidification of the medium, formation of chelating metabolites, or redox activity. The nutrient-solubilizing bacteria produce a halo zone around their colony, which was used to determine their solubilization capacity. The phosphate solubilizing fungus (PSF) secretes some kinds of organic acids which act on insoluble phosphates and convert the same into soluble form, thus providing phosphorous to plant. Ninteen of the rhizospheric soil sample were collected from the different region of the district Meerut and Muzaffarnagar and were sceened on PDA and PVK plates to obtain variety of fungal isolates. 17 fungal cultures were isolated from the 19 soil samples and 03 isolates were obtained from different studies. These 20 isolates were screened and selected on the basis of its ability to form halo (zone of solubilisation) zone on PVK medium plates. Out of 20 isolates 15 isolates were found to solubilise the TCPs. Further, colony morphology and microscopic studies shows that the 12 isolates (S2,S3.A,S5,S7.AS8,S10,S12,SS6.A,SS6.B ISOL.1,S4 and S7.B) belong to the genera Aspergillus, 04 isolate (S11,S13,S3.B,S7.C) belongs to the Trichoderma, 03 Penicillium (S1,S15 and ISOL.2) and 01 of Fusarium spp. 15 isolates (Aspergillus and Penicillium) showed the zone for P solubilisation while the other 05 fungal isolates (Trichoderma 04 no and Fusarium 01 no) did not show any zone of solublization. However, Trichoderma isolates showed production of acid in PVK medium which was evident by appearance of red colour zone surrounding the culture colony. Among all of the Aspergillus isolates maximum solubilisation efficiency (SE) was shown by S2 of 2.62 followed by SS6.B(2.33), S8(2.25), S3.A(2.08) and S7.A(2.00), the minimum (SE) was shown by isolate S12 of 0.43. While considering about the SI value isolate SS6.A had the maximum value of 1.19. Among all three Penicillium isolates tested for P solubilisation, ISOL.2 was recorded to have more efficiency (SE) of 1.08 than other isolates followed by S1(1.00) and S15 (0.58) on the twelfth day of observation. If considering about the solubilisation index (SI) then, ISOL.2 shows maximum SI value of 1.13 followed by S1(1.10) and S15(1.05). Comparatively among all the Aspergillus spp. S3.A, S10 and S2 can be considered as the best solubilisers while S15 and ISOL.2 were better among the Penicillium species. (Akash Tomar) (Deeksha Chaudhary) ADVISORThesisItem Open Access ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF SALMONELLA FROM UNPASTEURIZED SUGARCANE JUICE(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021) RUCHI BALIYAN; (Purushottam)ABSTRACT NAME RUCHI BALIYAN ID. NO. 4888 DEGREE MSc. BIOTECHNOLOGY Title “ Isolation and biochemical characterization of Salmonella from unpasteurized sugarcane juice.” Advisor Dr. Purushottam Fruit juice are extremely popular because of their nutritional worth, mineral richness and vitamin content as well as their affordibility. A lack of knowledge about hygienic practices procedures among vendors leaves goods such as fruit juices susceptible to higher bacterial contamination levels. Increased intake of fruit juice has a direct positive impact on economic status, but it may have detrimental impact as foodborne illness, outbreaks and spoilage issues occur. Globally there are instances of people becoming sick from fruit juices owing to foodborne illness. About 150-177 million people are expected to be affected by this illness by 2030 based on the no. of cases recorded in United States in 2001. Pathogens like enterohaemorrhagic E.coli, Salmonella, etc have all been found in juices. Salmonella is a Gram- negative, oxidase negative, rod- shaped bacterium belongs to the family enterobacteriaceae. It is a intracellular pathogen which can grow and reproduce inside the host cell. Salmonella leads to systemic disease in human body. The role of Salmonella in human illness has been recognized virtually since its discovery in 1885. Therefore, this study is planned out to evaluvate the presence of Salmonella in unpasteurized sugarcane juice. Which were collected from different regions of Shamli and Meerut. The samples were inoculated into T.T broth and incubated at 37° C for 24 hrs followed by streaking on MacConkey agar plates and then BSA plates followed by biochemical characterization. Total 15 samples out of 34 samples found to be positive for Salmonella as they display non-lactose fermenting white colony on BSA, and blue- green colour colony on HEA. A series of biochemical tests was performed, results of these test confirms the presence of Salmonellain the samples. The isolates showed sensitivity to levofloxacin, netillin, and norfloxacin. The zone of inhibition was 20 mm, 10 mm, and 20 mm respectively. And the isolates showed resistance to a no. of antibiotics like ampicillin, imipenam, ceftazidime, cefixime, etc. Antimicrobial effect of Haldi was also tested and Haldi have a significant effect on Salmonella, Hence, it is suggested that natural antimicrobials can be used as an alternate to antibiotics. The Salmonella isolates were resistant to many antibiotics. Therefore there is a need of measuring hygienic protocols and prescribed and limited use of antibiotics to prevent the transfer of resistance gene among the pathogen population. (Purushottam) Ruchi Baliyan Advisor CURRICULUMThesisItem Open Access EFFECT OF SUPPLEMENTATION OF TULSI (Ocimum sanctum) LEAF POWDER ON GROWTH PERFORMANCE IN COMMERCIAL BROILER(SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),, 2021-12) SURAJ GAJANAN MADAVI; Dr. RajkumarABSTRACT Name : Suraj Gajanan Madavi I.d. No.: 4825 Advisor : Dr. Raj Kumar Degree : M.Sc. (Ag.) A. H. Department : Animal Husbandry Thesis Title : Effect of supplementation of tulsi (Ocimum sanctum) leaf powder on growth performance in commercial broiler The present study was aimed to explore the effect of supplementation of tulsi leaf powder on growth performance in commercial broilers. A total of 160, day-old chicks (Vencobb-430 strain) were used for this study purpose and divided into four groups, and each group was divided into four replications, 10 chicks in each. The group first was offered a basal diet without any supplementation, which acted as a control and the second, third and fourth group was supplemented with dried tulsi leaf powder @ 0.5, 10.0, 15 g/kg feed, respectively for the 42 days of the study period. Bodyweight and feed consumption were recorded at weekly intervals. Bodyweight was significantly (P<0.05) higher in tulsi leaf powder supplemented groups as compared to the control group at 1st and 3rd weeks of the trial period. During the 2nd and 6th weeks of study, body weight was significantly higher in T1 as compared to control but no statistical differences were observed with T2 and T3 groups. However, body weight was reported significantly (P<0.05) higher in T1 and T2 groups in comparison to the control group at 4th and 5th weeks of the experimental period. Body weight gain was statistically (P<0.05) higher in the T1 group as compared to the control group from 1st to 6th weeks expect at 3rd week. Overall body weight gain at the 42nd day of experimental trail was statistically (P<0.05) higher in the T1 and T2 group as compared to control group. Feed consumption did not show any statistical differences among Control, T1, T2 and T3 groups at 1st week. However, feed consumption was reported statistically (P<0.05) different for the control, T1, T2 and T3 groups at 6th week. Feed conversion ratio was statistically (P<0.05) improved in tulsi leaf powder supplemented groups as compared to the control group during 42 days of tail period. The study was concluded that supplementation of dried tulsi leaf powder at 5g/kg feed in the broiler’s diets showed better body weight, body weight gain and improves feed conversion ratio compared to other treatment groups. Dr. Rajkumar Suraj Gajanan Madavi Advisor Author Suraj Gajanan Madavi Vill:- Sawalkheda Post:- Sawalkheda Tehsil:- Kurkheda Distt:- Gadchiroli (M.H.) Pin:- 441217 +918605034162 Suraj.madavi.336@gmail.com ACADEMIC QUALIFICATION
