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Subject: Animal Reproduction, Gynaecology and Obstetrics × Author: Sharma, Pururava × Start date: 2020 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Ultrasonographic evaluation of pre- and post-pubertal follicular dynamics in Sahiwal and Crossbred heifers
    (Palampur, 2022-12-07) Sharma, Pururava; Kumar, Pravesh
    The study was conducted to compare the pre-pubertal versus post-pubertal follicular dynamics in Sahiwal (n=10) and Jersey crossbred (n=10) heifers by using trans rectal ultrasonography. The investigation was carried out in the Livestock Farm Complex of the University from March, 2021 to August, 2022. Irrespective of the pre- or post-pubertal stage, the ultrasonography was done every other day in the both groups of heifers. In pre pubertal heifersthe day of emergence of follicular wave was considered as day 1. Thereafter, the observations were made for 21 days. Absence of luteal tissue was used as confirmation of pre-pubertal stage in both the groups of heifers. All the animals were re-examined for the initiation of puberty after every 20 days and monitored regularly for the exhibition of signs of estrus. In Sahiwal and Jersey crossbred heifers a significant variation (p<0.01) was observed in the body weight at birth (21.2±0.28 and 27.0±0.16 kg), body weight at puberty (335.9±5.75 and 366.5±4.52 kg) and age at puberty (1132.7±53.22 and 932.9±20.95 days), respectively. ROC curve revealed that pubertal weight in Sahiwal and Jersey crossbred heifers was ≥ 308.11 and ≥ 337.17 kg, respectively, with a specificity of 90 and 100 per cent. A significant variation (p<0.01) was recorded during post-pubertal period for back fat thickness in Sahiwal and Jersey crossbred heifers which were 12.86±0.05 and 12.69±0.01 mm, respectively. Follicular dynamics in pre- and post-pubertal stages occurred in a wave like manner. In Sahiwal and Jersey crossbred heifers, the preponderance of two- or three follicular waves were 40 or 60 per cent; 70 or 30 per cent, respectively. The inter-estrus interval in two- and three-wave Sahiwal and Jersey crossbred was 17.50±0.28, 17.85±0.34 and 20.83±0.40, 20.33±0.66 days, respectively which differed significantly (p<0.01) among different waves but not between the breeds. The size of pre-ovulatory follicle in two-wave Sahiwal and Jersey crossbred heifers differ significantly (p<0.01) but not in three wave cycles.