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Subject: Clinical Veterinary Medicine, Ethics and Jurisprudence × Author: ANJU K. DANIEL × End date: 2023 × Start date: 2020 ×
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    ThesisItemOpen Access
    DIAGNOSIS AND THERAPEUTIC EVALUATION OF MALASSEZIOSIS IN DOGS
    (Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, 2021-04-20) ANJU K. DANIEL; Vinu David P.
    Dogs presented at TVCC, Pookode with clinical signs suggestive of malasseziosis were screened by cytological examination and included in the study group. The prevalence of malasseziosis among dogs with dermatological problems was found to be 14.7 per cent. The highest prevalence of 36.7 per cent of malasseziosis was recorded in the age group of 1-3 years. German shepherd breed was found to be most affected. Females were affected more when compared to males. Alopecia, scaly lesions, foul odour and hyperpigmentation were the predominant clinical signs observed in dogs with malasseziosis. Haematological examination revealed a highly significant increase of TLC count, a significant decrease of Hb and VPRC values in dogs with malasseziosis upon comparison with control group. Serum biochemical study revealed a highly significant decrease in total proteins and globulin values and a significant decrease in albumin values and a significant increase of ALP and a highly significant increase of total bilirubin in the study group when compared to control group. Impression smear examination revealed the presence of blue coloured and footprint shaped budding yeast cells in all the positive cases. The isolation rate of malassezia yeasts was found to be highest on MDA with 76.7 per cent. Total DNA extracted from the pure cultures of the isolates were subjected to PCR targeting the LSU rRNA gene of Malassezia spp. and the amplicons were sequenced. Twelve sequences showed similarity to sequences of Malassezia pachydermatis and one sequence to Malassezia japonica. Molecular typing grouped all these 12 isolates of Malassezia pachydermatis into Type I sequences isolated from dogs and one isolate of Malassezia japonica to sequences from isolates of humans from India and Brazil. On clinical and cytological examinations, orally itraconazole and topically neem oil were found to be very effective by 28th day of treatment.