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  • ThesisItemOpen Access
    Physiological characterization of linseed (Linum usitatissimum L.) genotypes under salinity
    (CCSHAU, Hisar, 2023-10) Manisha; Anita kumari
    The present investigation entitled “Physiological characterization of linseed (Linum usistatissimum L.) genotypes under salinity stress” was investigated in the laboratory of Deptt. Of Botany and Plant Physiology. Salinity is one of the most important factors that restricts plant growth in many regions of the world. In order to assess how salt stress effects germination, growth and some physiological characteristics of flax, two separate experiments were carried out in the laboratory. The first experiment included fifty genotypes and three salinity levels (control, 8dS/m and 10dS/m). The results showed that with increasing salinity, germination percentage, seedling length, seedling fresh and dry weight and seedling vigour index decreased as compared to control. Similarly, the relative water content of shoot and root decreased with increasing salinity level from control to 10dS/m. At 10dS/m salinity level osmotic potential (ψs) of shoot and root also declined. Total chlorophyll content, chlorophyll stability index and total carotenoid content of shoot also decreased from control to 10dS/m salinity level. The level of osmolyte i.e. proline increased with increasing salinity level. A marked increase in hydrogen peroxide (H2O2) and lipid peroxidation (MDA) was noticed in both shoot and root of linseed genotypes. Enzymatic activity of antioxidative enzymes (SOD, CAT, POX, APX and GR) increased under salinity (10 dS m-1) stress. Despite, the increase in the activity of these enzymes ascorbate (AsA) and glutathione content decreased at 10dS/m respective to their control. The above observations suggest that salinity had a negative effect on morpho-physiological and biochemical parameters, ultimately resulting in a decrease in the growth of plants. Overall it is concluded that accession no. IC0268344, IC0499192 performed better under salinity stress so, these accessions may be considered as relatively tolerant.
  • ThesisItemOpen Access
    Evaluation of promising desi cotton (Gossypium arboreum L.) genotype and insecticidal management of bollworm complex
    (CCSHAU, Hisar, 2023-04) Manisha; Rolania, Krishna
    The present study was conducted at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana (India). Effect of different sowing time, varietal screening, insecticidal management and avoidable yield loss etc. were studied against bollworms in cotton during 2018 and 2019. In the variety, HD 432 (desi) incidence of bollworms namely, Earias spp (Earias insulana & Earias vittella), and Pectinophora gossypiella Saunders was high in May sown cotton (14th May) as compared April sown cotton (12th and 27th April). Weather parameters like minimum temperature, evening relative humidity and wind speed showed significant negative correlation with spotted bollworm and pink bollworm damage. Twenty one genotypes were screened during 2017 and 2018 and green bolls damage was recorded minimum in genotype IET (2) 5 against spotted bollworm (8.87 and 9.93%, respectively) and pink bollworm (0.67 and 0.80%, respectively) bollworm. While genotype CISAA 6 was observed with maximum incidence of spotted bollworm (15.91 &18.16%) and pink bollworm (4.13 & 4.80%) during 2018 and 2019, respectively. Bollworms incidence showed negative significant correlation with rind thickness, total soluble sugar and tannin content while positive significant correlation was observed with total phenol content and crude protein in seeds. During 2018 and 2019 twenty insecticidal treatments with control was evaluated against bollworms damage. Treatment T17 (spray of spinosad 45 EC initiated at 25% square formation) was found highly effective with minimum SBW damage (6.74 & 7.36 %) in green bolls that was statistically at par with T18 (spray of Spinosad 45 EC initiated at initiation of boll formation) (7.23 & 7.97%), T13 (spray of Thiodicarb 75 WP initiated at 25 % square formation) (7.33 & 7.95%) and T5 ( Spray of Bifenthrin 10 EC initiated at 25 % square formation) (7.37 & 8.03%) during 2018 and 2019, respectively. Pink bollworm damage on locule basis was also recorded minimum in T17 (spray of spinosad 45 EC initiated at 25% square formation) with 6.16 and 8.40 per cent damage during 2018 and 2019, respectively. Significant difference was observed between yield of protected and unprotected fields. Yield obtained in protected condition was 1750 and 1628 kg/ha while in unprotected condition it was 2593 and 2356 kg/ha during 2018 and 2019, respectively. Avoidable yield loss was calculated 32.52 and 30.91 percent during 2018 and 2019, respectively.