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ThesisItem Open Access Bio-fortification of Fe and Zn in rice (Oryza sativa L.)(CCSHAU, 2019) Jangir, Chetan Kumar; Sangwan, P.S.For the present investigation, fifteen surface soil samples from each North-Eastern district (10) of Haryana under paddy-wheat cropping system were collected using GPS to study the distribution of micronutrients (Fe and Zn), their contribution in paddy, release behaviour and finally the effect of Fe and Zn on their bio-fortification in rice. To achieve the objectives, a laboratory, screen house and field study was carried out. Laboratory and screen house study was conducted at CCS HAU, Hisar while field study was carried out at CCS HAU Rice Research Station, Kaul during kharif 2017 on loamy soil testing slightly alkaline in pH, low EC, low in available N, low to medium in available P, medium to high in available K. Field experiment in a RBD with five replications was laid out in a plot size of (5m x 4m) having five levels each of Zn [(0, 12.5 , 25.0 kg ha-1 as basal, foliar spray (0.5%) ZnSO4 at Panicle initiation & Heading stages and 25 kg ha-1 as basal + foliar spray (0.5% ) ZnSO4 at Panicle initiation & Heading stages] and Fe [0,50,100 kg ha-1 as basal, Foliar spray (0.5%) FeSO4 at 45 DAS and 100 kg ha-1 as basal + foliar spray (0.5%) FeSO4 at 45 DAS]. Results showed significant differences due to various treatments of Fe and Zn over their respective controls. The highest grain yield was recorded with combined application of 25 kg Zn ha-1 as soil application + foliar spray of Zn (0.5%) at Panicle initiation and heading stages which was significantly superior to (Zn 0.5% foliar spray @ 45 days after sowing which in turn on a almost par with the treatment of 25 kg Zn ha-1 as soil application alone. Likewise, with Fe fertilization grain yield also differed significantly with the treatments over control. The same was true for straw. The data on harvest index of Fe & Zn, and protein content revealed that their values differed significantly with various treatments of Zn or Fe over their respective controls. A screen house study also supported these findings. The laboratory study on fractionation of Zn and Fe showed that the different forms of Zn and Fe were mainly associated in the residual fraction and the order of preponderance of Zn fractions was Ex-Zn