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Subject: Genetics and Plant Breeding × Author: Deepak Kumar × Start date: 2017 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Combining ability and molecular diversity analysis in bread wheat (Triticum aestivum L.)
    (CCSHAU, 2019) Deepak Kumar; Panwar, I.S.
    The present study was carried out to draw out information on the nature of gene effects for yield and its components, genetic variability and assesment of genotypic variability among the parents using SSRs markers in wheat (Triticum aestivum L. em. Thell). The experimental material comprised nine genotypes viz. HD2967, HD3086, HD3059, Raj3765, WH711, WH283, WH1184, WH1105 and WH1124. A diallel set of 9 x 9 was prepared by crossing these genotypes in all possible combinations excluding reciprocals. Parents and their F1 generations were planted during rabi, 2017-18 in RBD (randomized block design) with three replications. The observations were recorded on five randomly selected plants in parents and F1s for thirteen traits. Combining ability analysis was performed by utilizeing method 2, Model-I of Griffing (1956) which indicated that the mean squares due to GCA and SCA were significant for all the thirteen traits. The magnitude of GCA component (gi2) was higher than SCA component (sij2) for plant height and peduncle length, indicating that these traits were mainly under the control of additive genetic variance. An overall appraisal of GCA effects revealed that HD3059and HD 2967 were good combiners for the most of traits. High GCA effects are related to additive gene effects or additive x additive interaction effect which represent the fixable genetic component of variation. For grain yield the crosses, HD2967 × Raj3765, HD2967 × WH711, HD2967 × WH1184, HD3086 × Raj3765, HD3086 × WH711were identified as promising on the basis of their high per se performance, average heterosis and heterobeltiosis and with high significant SCA effects. A total of 56 alleles were detected in and number of alleles per locus ranged from 1-4 with an average of 2 alleles per locus. NTSYS-pc based two dimensional PCA analysis showed scattering of the parents. Similarity coefficient among all the nine genotypes ranged from 0.62 to 0.85. Similarity coefficient revealed WH711 and HD3059 were the most diverse parents among the nine with low similarity index 0.62. WH1124 and WH1184 were showing highest similarity among the nine and highest similarity index 0.85. The findings of genetic similarity coefficient analysis illustrated that wide genetic diversity was present among parental genotypes which was used for making crosses.