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Subject: Biotechnology and molecular Biology × Author: Prexha × End date: 2023 × Start date: 2023 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Molecular characterization of wheat-Aegilops addition/substitution lines for grain β-glucan content
    (CCSHAU, Hisar, 2023) Prexha; Upendra Kumar
    β-glucan is one of the most important types of soluble dietary fiber proven beneficial for human health, including lowering serum cholesterol and ameliorating type-II diabetes. The 2% or higher β-glucan content in a daily diet is considered adequate for good health. In India, wheat is a staple food crop. To find out the best source of β-glucan, we explored a panel of 37 wild relatives of wheat, 209 accessions of tetraploid and hexaploid wheat, and 17 wheat-Aegilops addition/substitution lines with other associated quality traits. Among all these, high β-glucan content was found in Aegilops species (Aegilops kotschyi and Aegilops peregrina) ranged from 1.67-3.73%, in tetra and hexaploid wheat ranged from 0.30-1.31%, whereas in wheat-Aegilops addition/substitution lines ranged from 0.74-1.76%. The physical mapping of the putative gene(s) for β-glucan synthesis is still unclear. Therefore, one of the putative genes, TaCslF6 has been mapped on the long arm of chromosome 7D of the wheat genome. The physical mapping of TaCslF6 will be helpful for the genetic improvement of wheat for high grain β-glucan. The results of expression analysis of TaCslF6 and TaCslH (performed on different grain developmental stages i.e. 10, 20, 30 and 40 days after anthesis) revealed that gene TaCslF6 was relatively abundant during all the stages of development except 20 DAA. Whereas, a peak of TaCslH transcripts was found at 10 DAA in the selected wheat-Aegilops addition/substitution line (UPHAU-3). A wide genetic diversity found in the panel of 263 wheat genotypes gives ample scope to utilize its variability for crop improvement in population by applying specific and directed breeding approaches to create a “Magic Wheat” for global food and nutritional security.