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Subject: Biotechnology and molecular Biology × Author: Nikita × End date: 2023 × Start date: 2022 ×
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    ThesisItemOpen Access
    Identification and characterization of Meta-QTLs, ortho-MQTLs, and candidate genes for the traits associated with lodging tolerance in common wheat (Triticum aestivum L.)
    (CCSHAU, Hisar, 2023-05) Nikita; Upendra Kumar
    Lodging is an abiotic stress greatly affected by environmental conditions. It is a state of permanent displacement of stem from its vertical position which can reduce yield up to 80% in crops. A meta analysis of QTLs related to lodging and its associated traits was conducted in the present study to find the accurate and robust genomic positions concerning lodging tolerance in wheat. 86 MQTLs were identified using 1269 initial QTLs collected from 107 earlier studies. A high-density consensus map was generated containing 1,51,945 markers spanning a total length of 11,277.087 cM with an average marker density of 13.47 markers per cM length of map. The marker density for individual sub genome was 29.58 for A, 17.46 for B and 5.55 for D. In the resulting MQTLs there was a 4.13 fold reduction in confidence interval than the initial QTLs. 12 Breeders’ MQTLs were selected based on high PVE value, large LOD score, the greater number of initial QTLs involved and low confidence interval. 10 ortho-MQTLs were found using the Breeders’ MQTLs based on synteny and collinearity among wheat and maize. 678 gene models were found in the Breeders’ MQTL regions out of which 53 genes had an expression value of greater than 5 TPM. The most likely candidate genes belonged to the following families: F-box protein, SANT/Myb domain transcription factor, Pectinesterase inhibitor domain, glycoside hydrolase family, protein kinase domain, zinc finger domain, cytochrome P450, UDP-glucuronosyl/UDP-glucosyltransferase, glycosyltransferase 61 etc. 34 genes were found in wheat which orthologous of already reported genes encoding for lodging tolerance in rice, maize and Arabidopsis. This meta-analysis defines the most stable and consistent genomic locations associated with reliable molecular markers and candidate genes for lodging tolerance in wheat.