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Comparative studies of free and immobilized Aspergillus phytase
(CCSHAU, Hisar, 2021-09) Mallesh, Gampa; Nagar, Sushil
In this investigation the phytase from Aspergillus oryzae SBS50 was immobilized over activated aluminum oxide beads. The immobilization yield and efficiency were 47.4 and 71.2 %, respectively. Morphology and functional groups of activated beads and immobilized phytase were determined through SEM and FTIR analysis. The immobilized phytase retained 51.5 % of its initial activity even after 5 cycles of reusability. The optimum pH and temperature were shifted from 5.5 to 4.0 and 50 to 60 C after immobilization. The Km value was 2.61 and 3.09 mM, whereas as Vmax value was 32.25 and 43.29 IU/ml for free and immobilized phytase, respectively. The pH stability, thermal stability, storage stability and thermodynamic parameters (H, G, and S) were enhanced after immobilization The increase in Half-life (t1/2) and D-value whereas decrease in Kd value after immobilization indicates that thermal stability of phytase was enhanced. Thus, the investigation concludes that immobilization of phytase causes in improvement of many properties, so it is highly beneficial to immobilize the phytase.