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ThesisItem Open Access Induction of genetic variability through gamma rays and assessment of variants by molecular markers in rice (Oryza sativa L.)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-07-28) Jadhav Akshay Hanamant; Desai S. S.; Bhave S. G.; Mane A. V.; Sawardekar S. V.; Dalvi V. V.The present investigation entitled “Induction of genetic variability through gamma rays and assessment of variants by molecular markers in rice (Oryza sativa L.)” was carried with aim to create genetic variability by using different mutagenic treatments of gamma rays in rice cultivars, Chakhao, Bangalya and Ghansal. The trials were carried out during kharif 2021 and Summer 2022 at Botany farm, Department of Agricultural Botany, College of Agriculture and molecular analysis was carried out in the laboratory of Plant Biotechnology Centre, Dr. B. S. Konkan Krishi Vidyapeeth, Dapoli. Decreasing trend was observed for per cent germination in all mutagenic treatments with increased dose of mutagen under laboratory as well as under field condition. Similar trend was also recorded in germination per cent, shoot length, pollen fertility and spikelet fertility. Considering laboratory and field observation on per cent germination and other related parameters, LD50 dose was optimized. For chakhao and bangalya cultivars mutagenic treatment 300Gy and in case of ghansal cultivar 100 Gy doses were optimized as a LD50 dose. During M1 generation, early flowering, late flowering, sterile spikelet mutants were recorded. Four types of chlorophyll mutations viz. Albina, Xantha, Chlorina and Striata, were noticed in all mutagenic treatments. Mutation frequency, mutagenic efficiency and mutagenic effectiveness were reduced as per the increased dose of mutagen. Wide range of variation was observed in quantitative characters during M2 generation viz., days to fifty per cent of flowering, maturity duration, plant height, number of tillers per plant, number of spikelets per panicle and grain yield per plant. During M2 generation various phenotypic variants were recorded. Early maturing, dwarf stature, increased tillers and higher grain yield mutants were observed. Spikelet fertility decreases with increased dose of gamma radiation in all cultivars under study. Grain yield and straw yield also showed reduction over the control because of reduced height, reduced panicle length, increased spikelet sterility. The per cent polymorphism was estimated by using 10 SSR primers, almost all the primers showed higher PIC values. The higher the PIC value, the more informative is the SSR marker. Hence, RM-484, RM-154, RM-8225, RM- 5926, RM-152 and RM-474 were found to be more informative. Clustering pattern of dendrogram generated by using pooled molecular data of 10 primers of 39 mutants of each cultivar separately and it produced two main clusters Viz. I and II.ThesisItem Open Access GENETIC DIVERSITY STUDIES IN COWPEA (Vignaunguiculata(L.)Walp)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2020-09-30) TAMBITKAR, NARESH BHIVA; Pethe, U. B.The present experiment entitled “Genetic diversity studies in cowpea (Vignaunguiculata (L.) Walp)”was undertaken to assess the genetic variability, genetic divergence and path analysis in forty-one genotypes of cowpea in randomized block design with two replications at educational and research farm, Department of Agril. Botany, College of Agriculture, Dapoli, Dist. Ratnagiri, Maharashtra during rabi 2019-2020. The estimates of phenotypic, genotypic and environmental variance revealed that phenotypic variance were higher in magnitude than genotypic variance for all the characters. The magnitude of phenotypic and genotypic variance was closer to each other for majority of the characters thus indicating lesser role of environment in the expression of these characters. In general, phenotypic coefficient of variation (PCV) was higher in magnitude over genotypic coefficient of variation (GCV) for all the characters. Different characters showed varying per cent of coefficient of variation both genotypic and phenotypic levels. High genotypic and phenotypic coefficient of variation was observed for the characters iron content (ppm) , plant height at maturity (cm), seed yield per plant (%), days to maturity, dry matter yield per plant (g), while this were low for the characters days to fifty percent flowering. In present investigation, the genotypic correlation coefficient was higher in magnitude than their phenotypic counter parts for most of characters. Dry matter yield per plant and harvest index exhibited highly significant positive correlation with seed yield per plant both phenotypic and genotypic levels. The characters number of primary branches per plant, hundred seed weight, number of pods per plant, iron content, days to 50 % flowering, number of seeds per pod and protein content had non-significant positive correlation with seed yield per plant at both phenotypic and genotypic levels. Forty-one genotypes were grouped into 6 different clusters on the basis of magnitude of D2 values evaluated by Mahanlanobis D2 analysis.Among forty one genotypes, cluster I was comprising 12 genotypes followed by cluster II with 14 genotypes, cluster III with 5 genotypes, cluster IV with 4 genotypes, cluster V with 5 genotypes and cluster VI with 1 genotype. Inter cluster distance i.e. the maximum intra cluster distance was observed in cluster V (37.17) while the maximum inter cluster distance (117.49) was observed in cluster IV andclusters V. The seed yield is complex character and each character had its own effect for establishing correlation with yield. The path coefficient revealed that the character days to 50% flowering, number of pods per plant, plant height at maturity, dry matter yield per plant and harvest index had positive direct effect on seed yield at both phenotypic and genotypic levels, while days to initiation of flowering, days to maturity, number of primary branches per plant, pod length, hundred seed weight had negative direct effect on seed yield per plant at genotypic and phenotypic levels. It is concluded that IC-614758 followed by AKP-1264 and PGCP-27 are observed as best performance in the genotypes studied. These had highest seed yield per plant and also maximum yield attributing characters. The genotype CPD-31 had maximum seed weight; CP-13 had maximum number of seed per pod, while CP-25 had the maximum number of pods per plant. The genotype Phulevithai had highest protein content, CPD-219 had highest iron content and CPD-220 had minimum plant height. The genotype Konkansadabahar had early maturating. These genotypes can be used as promising genetic material in future breeding program. There was significant diversity reported in present study among all the genotypes.ThesisItem Open Access VARIABILITY AND CORRELATION STUDIES IN COWPEA (Vigna unguiculata (L.) Walp)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli , Dist : Ratnagiri, 2018) WAGHMARE, PANDURANG DILIP; PETHE, U. B.The present experiment entitled “Variability and correlation studies in cowpea (Vignaungiculata(L.) Walp)” was undertaken to assess the genetic variability, correlation and path analysis in thirty genotypes of cowpea in randomized block design with two replications at educational and research farm, Department of Agril. Botany, College of Agriculture,Dapoli, Dist. Ratnagiri, Maharashtra during Rabi 2016-2017. The estimates of phenotypic, genotypic and environmental variance revealed that phenotypic variance were higher in magnitude than genotypic variances for all the characters. The magnitude of phenotypic and genotypic variances was closer to each other for majority of the characters this indicating lesser role of environment in the expression of these characters. However plant height, dry matter yield per plant and days to maturity showed comparatively higher estimates of environmental variance indicating the influence of environment on these characters. Title of thesis : Variability and correlation studies in cowpea (Vignaunguiculata (L.) Walp) Name of the student : Mr. PandurangDilipWaghmare Regd. No. : 2488 Degree Discipline : : M. Sc. (Ag.) Genetics and Plant Breeding Name of the Research Guide : Dr. U. B. Pethe Jr. Spice Breeder AICRP on Spices, Dept. of Horticulture, College of Agriculture, Dr. B. S. K. K. V. Dapoli. Year of thesis submission : 2018 In general, phenotypic coefficient of variation (PCV) was higher in magnitude over genotypic coefficient of variation (GCV) for all the characters. Different characters showed varying per cent of coefficient of variation at both genotypic and phenotypic levels. High genotypic and phenotypic coefficient of variation was observed for the characters number of pods per plant followed by seed yield per plant, hundred seed weight and number of seeds per pod, while these were low for the characters days to maturity, days to fifty per cent flowering and days to first flowering. In the present investigation, the genotypic correlation coefficients were higher in magnitude than their phenotypic counterparts for most of the characters. The characters dry matter yield per plant, harvest index, hundred seed weight, number of pods per plant, number of seeds per pod exhibited highly significant positive correlation with seed yield per plant at both phenotypic and genotypic levels. The character, plant height showed significant negative phenotypic correlation with seed yield per plant. The seed yield is complex character and each character had its own effect for establishing correlation with yield. The path coefficient analysis revealed that thecharacter days to fifty per cent flowering, plant height, number of seeds per pod, dry matter yield per plant, hundred seed weight and harvest index had positive direct effect on seed yield per plant, while number of primary branches per plant and days to maturity had negative direct effect on seed yield per plant at genotypic and phenotypic level. It is concluded that genotype PCB-9796 followed by CPD-118 and RC-101 were the promising among all the genotypes studied on the basis of seed yield per plant by considering all yield attributing characters. The genotype PCB-9796 is bold seeded with maximum hundred seed weight, KBC-9 had maximum number of seeds per pod, whileMrugraj had the maximum number of pods per plant. These genotypes can be used as promising genetic material in future breeding program.ThesisItem Open Access “MORPHO-PHYSIOLOGICAL AND BIOCHEMICAL TRAITS ASSOCIATED WITH GROWTH, YIELD AND QUALITY OF LABLAB BEAN (Lablab purpureus (L.) Sweet)GENOTYPES UNDER VARYING RABI CLIMATIC CONDITIONS IN KONKAN REGION”(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli , Dist : Ratnagiri, 2018) JADHAV, SOMNATH MARUTI; Burondkar, M. M.The present investigation entitled “Morpho-physiological and biochemical traits associated with growth yield and quality of lablab bean (Lablab pupureus (L.) Sweet) genotypes under varying rabi climatic conditions in Konkan region”was conducted at Educational and Research Farm, Department of Agricultural Botany, College of Agriculture, Dapoli, during rabiseason 2015-2016 and 2016-2017. The investigation was aimed at morphological, physiological and biochemical characterization of five lablab bean genotypes (viz. genotype 63, genotype 83, genotype 54, genotype 84 and a check Konkan Wal-2) in three sowing dates (viz. 5th Nov, 15th Nov and 25th Nov)under Konkanrabi condition; with an object to study extent of thermo and photosensitivity of different lablab bean genotypes; study the effect of different sowing dates on yield and quality of lablab bean genotypes and identifying the morpho-physiologically efficient and consistent lablab bean genotype for higher yield under Konkanrabi conditions. The whole experiment was analyzed in split plot design in four replications. Results indicated that, among the three sowing dates, sowing date S1(5th Nov)owing to high accumulation of GDD (1913.2) and heliothermal units (8116.4), exhibited better performance than delayed sowing and recorded significantly higher plant height (2.97 %), leaf area (44.60 %),LAI(25.60 %), higher absolute growth rate (27.51 %),relative growth rate (28.57 %), net assimilation rate (12.75 %) specific leaf weight (42.18 %)along withhigher photosynthesis (9.28 %),chlorophyll content (23.81 %),chlorophyll stability index (38.57 %),water use efficiency (17.26%), carbon use efficiency (8.20 %), higher enzyme activities as higher α-amylase(6.10 %),β-amylase (2.66 %) and nitrate reductase (39.85 %). Whereas reduction of 23.91 % in catalase activitiy,8.20 %in stomatal conductance and56.56 % inproline content was evident in sowing time S1over late sowing S3.Among the five genotypes tested, genotype G3 was found to be significantly superior to all the other genotypes,recording significantlyhigher leaf area (33.45 %), LAI (7.46 %),higher absolute growth rate(12.51 %),relative growth rate(11.33 %),net assimilation rate(5.20 %),specific leaf weight(5.69 %),rate of photosynthesis(2.39 %),chlorophyll content(18.57 %),chlorophyll stability index(17.30 %),proline content(56.56 %),higher α- amylase (13.91 %),β-amylase (15.38 %), catalase(29.31 %) and nitrate reductase(25.00 %) than check genotype Konkan Wal-2. Whereas plant height was maximum and significantly 4.97 % higher in genotype G1over check genotype Konkan Wal-2.Among three sowing dates, S1 (5th Nov.), which was at par with S2 (15th Nov.), registered significantly 6.91% and 5.35 % more yield over late sowing S3 (25th Nov.), respectively.Genotype G3, proving to be significantly superior to all the other genotypes, recorded higher No. of pods per plant (11.00), higher No. of seeds per pod (4.04), 100-seed weight (20.65 g) and there by recorded higher seed yield per plant(9.08 g/plant).Highest straw yield per plant (36.66 gm)along with higher harvest index (23.64 %) was also observed in Genotype G3. Among the 5 genotypes of lablab bean tried in 3 windows of sowing, genotype G1 was found most consistent registering minimum deviation in seed yield from S1(5th Nov.) sowing time to S2(0.89 %) and S3(4.80 %) as compared to other genotypes and proved to be comparatively more thermo and photo- insensitive genotype. A strong positive correlation of seed yield/plant was observed with net assimilation rate (0.773),followed by harvest index (0.394). Therefore, these traits could be positively considered for yield improvement under climatic changes.ThesisItem Open Access EFFECT OF BIO-CONTROL AGENTS ON PHYSIO-CHEMICAL ASPECTS OF WAL (Lablab purpureus L.) (Accession No. T06466)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli , Dist : Ratnagiri, 2018-05) DHANSING, ADSUL VISHAL; Mane, A.V.A field experiment was conducted at education and research farm, Dept. of Agril. Botany, College of Agriculture, Dapoli during rabi 2017-2018 to study effect of biocontrol agents on physiochemical aspects of Wal (Lablab purpureus L). Different bio control agents were applied through foliar spray at 2st and 4th week after sowing. The experiment laid out in randomized block design with seven treatments randomized inthree replications. The apllied seven treatment VIZ T0 RDF only, T1 Rhizobium treatment, T2 RDF+ T1, T3 Tricoderma viride (4ml/lit)+ T1, T4 Pseudomonas fluorescence (4ml/lit)+ T1, T5 Bacillus subtilis (4ml/lit)+ T1, T6 Paceilomyces lilacinus (4ml/lit)+ T1 respectively. Beside yield and yield attributes data were collected on plant height, number of branches, number of leaves, days to 50% flowering, days to maturity, chlorophyll content, nitrogen content, phenol content, proline content, relative water content, Dry matter accumulation, leaf area, LAI, AGR, RGR, and NAR at the interval of 30, 60, 90 DAS and at physiological maturity. Result indicated that most of the yield and yield determining attributes recorded significant difference due to treatment effects. Among the treatments T6 (Paceilomyces lilacinus) recorded significant difference on yield and yield attribute, morphological, growth, physiological and biochemical parameter. The plant height was increased significantly in treatment T6. The significant increase in leaf dry weight, stem dry weight and total dry weight were observed in treatment T6 (Paceilomyces lilacinus). The number of days required for days to 50% flowering and days to maturity significantly reduced with the application of Paceilomyces lilacinus. Growth parameters viz. leaf area, leaf area index, RGR, AGR, LAI and NAR were significantly lower in control and the application of Paceilomyces lilacinus have been found effective in increasing parameters. The application of biocontrol agent enhanced physiological and biochemical parameters i.e. photosynthesis, stomatal conductance, chlorophyll content, phenol content, nitrogen content and relative water content the effect was more with application of Paceilomyces lilacinus. All biocontrol agent increases the accumulation of phenol but among them Paceilomyces lilacinus is superior rest of treatment. There was no any significant difference in proline accumulation among the treatments. The application of bio control agent enhanced the yield and yield attributing character i.e number of pods, pod yield per plant), Pod yield per plot, seed yield per plot, harvest index and seed yield per plot. The effect was more with application of Paceilomyces lilacinus followed by Pseudomonas fluorescence treatment. Seed yield per plot ranged between 772.45g and 510.17g maximum and minimum value being in T6 and T0 respectively. It shows variation between treatments. Among all treatments T6 (Paceilomyces lilacinus) was significantly superior treatments in all characters.ThesisItem Open Access STUDIES ON HETEROSIS AND COMBINING ABILITY FOR YIELD AND YIELD ATTRIBUTING TRAITS IN GREEN GRAM (Vigna radiata L. Wilczek) (Accession No. T06464)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli , Dist : Ratnagiri, 2018-05) BICHUKALE, MAHESH SHAMRAO; Devmore, J. P.The present investigation was carried out with a view to study the magnitude of heterosis, combining ability and gene action through Line x Tester analysis including ten parents, including one check and their twenty four F1 in green gram for Rabi 2017-18 conditions. The experimental material was planted in randomized block design with three replications at the research and educational farm, Department of Agriculture Botany, College of Agriculture, Dapoli. The analysis of variance for all the characters revealed that parents were found to be highly significant for all the character studied except branches per plant, pods per cluster and pods per plant indicating presence of considerable amount of genetic variability in the parental material tested. Also, all the yield contributing characters showed highly significant mean square due to hybrids vs parents‟ and 133 hybrids were highly significant for all the characters, Parents vs. hybrids comparison was found to be highly significant for all the characters studied except hundred seed weight indicating presence of heterotic combination. Among the Parents, TARM-2, Ml-2056, TARM-1, and Karjat local were recorded as the best performing parents for grain yield per plant. High heterotic effects were observed for days to 50 per cent flowering, days to maturity, number of branches per plant, number of clusters per plant, number of pods per cluster, number of pods per plant, pod length and grain yield per plant. It was observed from the present study that heterotic response for seed yield per plant was mainly due to high heterotic response observed for number of branches per plant, number of pods per plant, pod length and number of clusters per plant, number of pods per cluster. Combining ability analysis revealed that the mean squares due to general combining ability and specific combining ability were highly significant for almost all the characters indicating importance of both additive as well as non-additive gene effects involved in the expression of all the characters. However, the variances due to general combining ability in general were lower than specific combining ability for days to maturity, plant height, branches per plant, clusters per plant, pods per plant, grains per pod, hundred seed weight and grain yield per plant thus on the basis of ratio of gca and sca results pointing out the preponderance of non-additive gene effects for the characters studied. The general combining ability effects revealed thatML-2333, ML-2056, TARM-1 and Karjat localwere the good general combiner for grain yieldand other yield related traits. The crosses ML-2333 x TARM-1,NVL-641 x TARM-1 andNVL-641 x Karjat local were found to be good 134 in respect of sca effects for yieldrelated characters. This might be due to effect of alleles of combining parents. Out of these best specific cross combinations, majority of the crosses showed high per se performance along with higher heterotic effects and higher sca effects for seed yield per plant indicating more reliability of per se performance. The per se performance of parents were more or less related to their gca effects. On the basis of per se performance, heterosis, combining ability, and gene action of the hybrids viz., ML-2333 x TARM-1, NVL-641 x TARM-1, PUSA-1477 x TARM-1, ML-2056 x TARM-1andML-2056 x Karjat local was found to be the most promising combination for most of the yield contributing traits viz., grain yield per plant (g), number of pod per plant, number of clusters per plant, number of pods per cluster, hundred seed weight (g), plant height, number of pods per plant and pod length (cm).ThesisItem Open Access EFFECT OF BIO-CONTROL AGENTS ON PHYSIOCHEMICAL ASPECTS OF COWPEA (VignaunguiculataL.)(DBSKKV DAPOLI, 2018-05-20) LAGAD, SANKET KAILAS; Deshpande, R. S.A field experiment was conducted at education and research farm, Dept. of Agril. Botany, College of Agriculture, Dapoliduring rabi 2017-18to study the effect of different biocontrol agents viz.Trichodermaviride(Fungal strain), Pseudomonas fluorescens(Bacterial strain), Bacillus subtils(Bacterial strain),Paecilomyceslilacinus(Fungal strain), Rhizobiumleguminosarum(Bacterial strain) onphysio-chemical aspects of cowpea (VignaunguiculataL). The experiment was laid out in randomized block design with seven treatment randomized in three replications. The treatment effects were studied for various parameters viz. plant height, no of branches,number of leaves, days to 50 percent flowering, days to physiological maturity, dry matteraccumulation, leaf area per plant, leaf area index, absolute growth rate, relative growth rate, net assimilation rate, photosynthesis rate, transpiration rate, stomatal conductance, relative water content, total chlorophyll content of leaves, proline content of leaves, phenol content of leaves, nitrogen content of leaves, number of pods per plant, number of seeds per pod, pod yield per plant, pod yield per plot, seed yield per plot and harvest index. Data collected were subjected to analysis of variance. Most of the yield and yield determining attributes recorded significant difference due to treatment effects. Maximum (57.44cm) plant height was recorded in treatment T6(Paecilomyceslilacinus)while maximum (11.66) branching was recorded in treatment T6(Paecilomyceslilacinus). Number of leaves were ranged from 31.44 to 36.89.Early flowering (39.67 days) and maturity(76.16 days)was recorded in treatment T6(Paecilomyceslilacinus). The maximum (13.436 g per plant) and minimum (9.142 g per plant) total dry matter was recorded in treatmentsT6(Paecilomyceslilacinus) and T0 (recommended dose of fertilizeronly) respectively. Maximum (48.98 dm² plant-1) and minimum (33.50 dm² plant1) leaf area was recorded in treatmentsT6(Paecilomyceslilacinus) and T0 (recommended dose of fertilizeronly) respectively. Similarly, treatmentsT6(Paecilomyceslilacinus) and T0 (recommended dose of fertilizeronly) recorded highest leaf area index. The maximum AGR was recorded in 30 to 45 DAS facilitating the highest vegetative growth of a crop. In 30 to 45 DAS it ranged between 0.262g day-1 and 0.385g day-1. RGR was recorded highest in between 15 to 30 DAS and ranged between 0.0369 g g-1day-1and 0.0462 g g-1day1. NAR was higher for the period of 15 to 30 DASand it ranged between 0.0000246 g dm-2 day1 and 0.000230 gdm-2day-1.At 60 DAS, rate of photosynthesiswas ranged between 15.33 µmol CO2 m2s-1and 20.83 µmol CO2 m-2s1while stomatal conductance wasranged between 0.227 µmol m-2 s-1 and 0.497 µmol m-2 s-1.Transpiration rate increased with advancing age of the crop andranged between 5.17 µmol H2O m2s-1 and 7.50 µmol H2O m-2 s-1. RWC recorded highest at 15 DAS while lowest at 60 DAS. During 60 DASmaximum chlorophyll(1.70mg/g), phenol (24.97mg/g)and nitrogen (2.03%)content recorded in treatmentsT6(Paecilomyceslilacinus).Treatment differences were non-significant for the proline at all growth stages. Although the proline levels elevated concomitantly with growth stages. At 60 DAS proline ranged between 0.53μmol/g and 0.55 μmol/g. Maximumnumber of pods (14.50), number of grains per pod(10.13),pod yield per plant(29.00 g),pod yield per plot(1507.33 gm), seed yield per plot(1076.67g) and harvest index(43%) was posted bytreatmentsT6(Paecilomyceslilacinus).Among all treatments, treatment T6(Paecilomyceslilacinus) was recorded superior for yield and all yield contributing characters.ThesisItem Open Access PHYSIOLOGICAL APPROACHES FOR DROUGHT MITIGATION IN GROUNDNUT (Arachis hypogeaL.) CROP (Accession No. T06271)(DBSKKV DAPOLI, 2017-05-17) THOMBARE, ASHWINI DADASO; Mane, A. V.A field experiment was conducted at agricultural research botany farm, College of Agriculture, Dapoli to study the physiological approaches for drought mitigation in groundnut (Arachis hypogea L.) crop during Rabi 2016-17 seasons. Stress mitigating compound at different concentration were applied through foliar spray after 24 DAS and spraying were imposed immediately after irrigation for stressed plots and observations were taken before every irrigation. The experiment consisted of 12 treatment laid out in randomized block design with three replications. The experiment consisted of 12 treatment comprising KCl (1.0%), triacontanol (2ml/l), Methanol (2%), Alachlor (20ppm), Atrazine (100ppm), Kaolin (6%), CCC (100ppm), Nitrobenzene (20ppm) and salicylic acid (500ppm) and Water were imposed at 24 DAS in stressed treatments and applied at leaves (foliar spray). Among the treatments, control (regular watering at 7 days interval) and foliar application of triacontanol (2ml/l) recorded significantly higher in morphological, physiological, biochemical parameters and in yield and yield components over all the treatments, whereas, stress plot (T2)recorded lower values for these parameters as compared to other treatments. After control, foliar application of triacontanol recorded high values for morphological, physiological, biochemical parameters and in yield and yield components over all the treatments. Application of stress mitigating compound have been found effective in increasing these parameter. The LAD, relative water content, found to be maximum in control and increased bythe foliar application of triacontanol (2ml/l). The increased rate of photosynthesis and chlorophyll content has been achieved by the foliar application Triacontanol (2ml/l). Chlorophyll stability index was found to be increased by the foliar application of Triacontanol (2ml/l). The transpirational loss is reduced by application of Kaolin (6%).Days required for maturity was on time for control and foliar application of triacontanol (2ml/l).Proline content at different stages indicated that control had significantly lower proline content as compared to all other treatments whereas stressed plot (T2) had the maximum proline content. Leaf temperature and canopy temperature were found to be maximum in stress as well as foliar application of A Other yield and yield attributing characters viz. Days required for 50% flowering, number of pods, peg to pod ratio, harvest index, number of shrivelled pod and yield per plant was significantly increased by the foliar application of triacontanol (2ml/l). The oil and protein content was significantly higher in control and lower in stress plot as compared to other treatments.ThesisItem Open Access INFLUENCE OF PLANT GROWTH REGULATORS ON PHYSIOLOGICAL BEHAVIOR OF RICE (Oryza Sativa L.) UNDER KHARIF KONKANCONDITIONS. (Accession No. T05511)(DBSKKV DAPOLI, 2014-06-18) JADHAV, SOMNATH MARUTI; Burondkar, M. M.A field experiment was conducted at agricultural research botany farm, College of Agriculture, Dapolito study Influence of plant growth regulators on physiological behavior of rice (Oryza sativa L.) under kharif Konkan conditionduring Kharif 2013-14 seasons. Plant growth regulators at different concentration were applied through foliar spray at 30, 60, 90 and 120 DAS. The experiment consisted of 11 treatment laid out in randomized block design with three replications.The experiment consisted of 11 treatment comprising Triacontanol (10, 15and 15 ppm), CPPU (1, 2 and 3 ppm), salicyclic acid (15, 20 and 25 ppm) and paclobutrazol (50 ppm)treatment were imposed at 30, 60, 90 and 120 DAS. Among the treatments foliar application of salicylic acid @ 15 ppm and foliar application of triacontanol @ 15ppmrecorded significant difference on morphological, growth, physiological and yield parameters as compared to other treatments.Morphological characters such as plant height, number of leaves and leaf area significantly increased by the foliar application of triacontanol @ 15 ppm, whereas number of tillers and leaf area index was significantly increased by the foliar application of salicylic acid @ 15 ppm. Growth parameters were significantly lower in control. Application of plant growth regulators have been found effective in increasing these parameter. The foliar application of triacontanol @ 15 ppmincreased AGR, RGR and LAR, whereas NAR and LAD found to be increased by the foliar application of salicylic acid @ 15 ppm. The increased rate of photosynthesis and stomata conductance and reduced rate of transpiration have been achieved by the foliar application of salicylic acid @ 15 ppm. Chlorophyll content was found to be increased by the foliar application of triacontanol @ 15 ppm. Days required for maturity was reduced and straw yield was increased by the foliar application of tricontanol @ 15 ppm. Other yield and yield attributing characters viz. Days required for 50% flowering, number of panicles, length of panicles, number of fertile grains, 1000 grain weight and yield per plant was significantly increased by the foliar application of salicylic acid @ 15 ppm.
