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Open Access
Effect of processing and freezing procedures on the acrosome morphology of buck spermatozoa
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1998) Ranjini, A; KAU; Prabhakaran Nair, K
Six pooled semen samples (two ejaculates) of good quality from five Malabari crossbred bucks were processed and frozen in two different protocols to evaluate the effect of processing and freezing procedures on the acrosome morphology of buck spermatozoa. In protocol I, the samples were diluted 10 fold in Tris buffer before centrifuging twice and the final pellet was re-suspended in the non glycerolated fraction of Tris yolk diluent. The sample was glycerolated (six per cent), equilibrated (four hours), frozen (eight minutes), and thawed (250 C for 30 seconds). In protocol 11, centrifugation was done only once, after 15 fold dilution in Tris buffer. The re suspended pellet was glycerolated (seven per cent), equilibrated (three hours), frozen (10 minutes) and thawed (60° C for 10 seconds). The semen characters such as motility, live sperm, sperm abnormalities and acrosome abnormalities were evaluated at the end of washing and initial extension (stage I), cooling to 5° C (stage II), glycerolisation and equilibration (stage Ill) and freezing and thawing (stage IV). The results were compiled to evaluate the effect of different processing and freezing procedures on the semen characters in general and acrosome morphology in particular. The semen sample used for split sample dilution had a mean volume of 1.3282± 0.067 ml, creamy in colour, DDDD density, ++++ mass activity, pH of 7.275 2± 0.040 and a concentration of 2972 2± 293 millions per ml. No significant difference in the above semen characters were found between bucks. The initial sperm motility of 82.000 2± 0.606 was found to drop significantly during processing and freezing and the final post thaw motility obtained was 44.000 2± 0.790 in protocol I. Similarly in protocol II the initial motility dropped from 81.375 2± 1.089 to 44.750 2± 1.075 at the end of stage IV. Even though there was significant drop in motility between stages in both the protocols, there was no significant difference in the corresponding stages of the two protocols. It could be inferred that good post thaw motility was obtained in both the protocols. The fact that a single washing and centrifugation was only adopted in protocol II makes it a more acceptable procedure for buck semen freezing. The mean live sperm percentage of fresh semen was evaluated using both NE and NEG staining technique. The percentage of live sperms of 90.050 2± 0.801 was found to decrease to 54.250 2± 0.593 after freezing and thawing in protocol by NE staining. Similarly in protocol 11, the mean percentage of live sperms was found to reduce to 53.125 2± 0.793 with the same staining. Even though there was significant difference in the live sperm percentage between stages within protocol I and II no significant difference in the live sperm percentage between the corresponding stages of protocol I and I I . With NEG staining the initial live sperm percentage of 80.850 ± 1.494 was found to drop to 54.875 ± 0.677 in protocol I as against 53.400 ± 0.730 in protocol II. While there was significant difference in the live sperm percentage between stages within protocol I and II there was no variation between corresponding stages of the two protocols. A significantly lower percentage of live sperms was recorded with NEG staining when compared with NE staining probably on account of the fact that the differentiation of live and dead sperm was difficult in the former staining method as live sperms were stained light blue instead of colourless. The mean percentage of abnormal sperms of 3.050 ± 0.245 in fresh semen did not register any significant increase during processing. However, there was significant increase in the percentage of sperm abnormalities during freezing and thawing with the final abnormality percentage of 7.125± 0.706 in protocol I and 6.300± 0.36 in protocol II. The initial acrosomal abnormality of 8.825 in the fresh semen steadily rose to 23.375 in protocol I as against 19.825 in protocol II at the end of stage IV. There was no significant difference in the percentage of various acrosomal abnormalities between corresponding stages of the two protocols. However, there was significant increase in the acrosomal abnormalities during glycerolisation, equilibration, freezing and thawing under both the protocols. It was concluded that the processing and freezing under two different protocols did not significantly alter the post thaw motility, percentage abnormal and dead sperms and acrosomal abnormalities. A good post thaw motility and low acrosomal abnormality was obtained with a single washing of buck semen with 15 fold Tris buffer which was comparable with double washing with 10 fold Tris buffer.
Open Access
Evaluation of multi x bi bivoltine hybrids of silkworm
(1998) Rajeni, Narayanan; KAU; Ranjith, A M
In a study undertaken to evaluate the region and season specific multi x bi and bivoltine hybrids of silkworm Bombyx mori, for commercial exploitation in Kerala, six hybrids of silkworm were reared using K-2 and S-36 mulberry varieties under Vellanikkara condition. The crosses used were PM x NB18, PM X NB4D2 and their reciprocals, NB4D2 x NB18 and its reciprocal and PM as check. The evaluation was carried out based on characters like leaf consumption, larval duration and weight, cocoon and shell weights, shell ratio percentage, average filament length, denier, reelability and renditta. The climatic factors were also correlated with major economic characters of silkworm. The present study confirms the superiority of bivoItine silkworm hybrids like NB4D2 x NBI8 and NB18 x NB4D2 justifying their wide acceptance in states other than Kerala. It is evident that if rearing is done in a systematic way, by strictly adhering to the brushing period in correlation with the seasons and if the use of high quality mulberry varieties is advocated these crosses will yield good returns in Veil ani kkara. Though the various economic parameters reflected a variable trend, ultimately when the average filament length/g cocoon, shell ratio percentage and cocoon weight were considered, the bivoltine hybrids performed superior to the others.
Open Access
Land evaluation and suitability rating of the major soils of Onattukara region
(Department of soil science and agricultural chemistry, College of Agriculture, Vellayani, 1998) Premachandran, P N; KAU; Subramonia Iyer, M
The need for a scientific approach in inventorying and utilizing land resources most economically and efficiently is now universally accepted. Agricultural prosperity, to a great extent, depends on judicious use of soils and rational application of soils data. A reconnaissance soil survey of Onattukara region was undertaken to study the extent and distribution of the different soils for soil classification, land capability and irrigability classification and for subsequent land evaluation. The study reveals that Onattukara region extends over an area of 40,948 ha. The soil map showing the distribution of the identified twenty soil series has been prepared. The climatological data reveals that the soil moisture regime is , ustic and the soil temperature regime is isohyperthermic. The soils have been classified as per Soil Taxonomy (U.S. Soil Survey Staff, 1975) and Keys to Soil Taxonomy( U.S. Soil Survey Staff, 1994 and 1996). Ten series were classified under Entisols, SIX under Inceptisols, three under Ultisols and one under Alfisols. Based on the inherent soil characteristics and landscape features, the soils have been grouped into three land capability classes, nine capability subclasses and land capability map prepared. The soils were classified into five land irrigability classes, eight land irrigability subclasses and land irrigability map prepared. The soils were evaluated based on principles of land evaluation and rating of productivity parameters. The productivity rating of the soil propertIes agamst the productivity index for nee, coconut, sesamum ,cassava and banana were made and conclusions arrived at. Productivity rating shows that Kottakakam series is best suited for rice, Palamel, best for coconut and banana, Artuva and Mahadevikad, for sesamum and Palamel and Vallikunnam, for cassava. On the basis of the studies carried out, a land use is proposed for the twenty soil series of Onattukara region based on crop suitability ratings and crop suitability map prepared for the five crops taken for study. A proper soil survey interpretation provides information on soil potential, productivity and limitations in their sustained use. Soil survey information forms the major basis for land evaluation. A thorough knowledge of the potentialities and limitations of every piece of land is a prerequisite in its efficient utilization. A systematic survey is essential for the evaluation and classification of the soils based on their inherent soil characteristics, land capability, land irrigability and land suitability. A systematic survey and evaluation of the soils of Onattukara region was taken up to study, interpret, classify and to show their location and extent on base maps. It is hoped that the present study would open up avenues for further investigations on land evaluation, crop suitability and other management aspects for sustained use of soil resource data to the best advantage. This will also form the basis for microlevel planning for integrated and sustainable development of the region under Panchayat raj.
Open Access
Management of foot rot of black pepper (piper nigrum L.) with va mycorrhiza and antagonists
(Department of Plant Pathology, College of Agriculture, Vellayani, 1998) Christin Robert, P; KAU; Sivaprasad, P
Extensive investigation was carried out to develop a native microbial inoculant based technology involving Arbuscular Mycorrhizal Fungi (AMF) and fungal antagonists for the foot rot disease management and growth improvement of black pepper in the nursery and field. Phytophthora capsici Leonian emend A. Alizadeh and P.H. Tsao, the foot rot pathogen isolated from Peringammala, Thiruvananthapuram district was found most virulent isolate. Seven native AMF cultures and fifty fungal antagonists were isolated from Kerala soils. AMF isolates were screened in the green house for plant growth improvement and disease tolerance in comparison with identified species-Glomus fasciculatum, G.clarum and Gigaspora margarita. Of the ten AMF tested isolates Is - 6, Pi - 11, Pi - 9, G. fasciculatum and Gigaspora margarita were very effective in stimulating growth and nutrient (P, K, Ca, Mg, Cu, Fe, Mn and Zn) uptake of black pepper. Regarding the ability of AMF in reducing the foot rot incidence, Glomus fasciculatum recorded the lowest plant mortality and root rot index (53.35% and 62.50%) followed by Is - 6 (60.00% and 64.77%) and Pi - 11 (60.64% and 68.18%) as against 100 per cent mortality and 98.60 per cent root rot index noticed in control. The above five cultures were subjected for further studies. Characterisation of AMF associated with different genotypes of black pepper grown in various soil types indicated the definite influence of soil type on AMF colonization. Sandy soil (oxyaquic quartpsamment) harboured maximum root colonization while forest soil (haplic argiustoll) had the lowest. Species of Glomus particularly G. fasciculatum was the predominant AMF associated with black pepper irrespective of soil type. As an exception Acaulospora and Gigaspora species were frequently noticed in sandy soils. Based on the ability of the fungal antagonists to suppress P. capsici in vitro either through mycoparasitism, antibiosis or soil fungistasis, 24 isolates were selected for green house studies. In the further testing isolates A1, A13, A21, A22and A35 significantly reduced the foot rot infection and increased the plant growth. They showed better population build up in the soil and suppressed the P. capsici population considerably. These native antagonists were further tested in combination with selected AMF in the green house and field. Under green house condition, combination of G. fasciculatum x A1 or A21 showed significant influence on growth stimulation, while Is - 6 x A22 recorded lowest mortality of 32.90 per cent due to foot rot incidence as against 97 per cent in control. The dual inoculation of Is - 6 x A21 and Pi - 11 x A1 was highly effective in plant growth stimulation and disease suppression. Both the combination recorded less than 60 per cent infection and mortality due to the disease, while control showed 95.66 per cent infection and plant mortality. Bordeaux mixture and copper oxychloride recorded 66.67 and 59.68 per cent mortality respectively. AMF colonization and multiplication of antagonists were also favoured by dual inoculation. The potential AMF isolates Is - 6 and Pi - 11 were identified as species of Glomus while, the antagonistic isolates A1, A13, A21, A22, and A35 were confirmed as aspergillus fumigatus Fres., Fusarium oxysporum Schlecht. Ex Fr. Aspergillus sydowii (Bain. & Sart.) Thom. & Church, Trichoderma viride Pers. Ex Gray. And Gliomastix murorum (Corda) Hughes respectively. A technique for AMF inoculation to established pepper vines was developed using ‘carrier plants’. Raising sorghum with AMF inoculation around the pepper vines was found effective to achieve intense colonization in pepper roots by the introduced AMF in the field. This technique developed for the pepper vines may be tried for extending to other perennial crops for AMF inoculation. Promising AMF cultures Pi - 11, Is - 6, G. fasciculatum and antagonists Aspergillus fumigatus, A. sydowii, Trichoderma viride were further tested on eight year old established pepper vines following ‘carrier plant’ based AMF inoculation and cowdung - neem cake based antagonist inoculation. The treatment Pi - 11 x A. Sydowii was most effective with no symptom development, followed by Is- 6 x T. Viride or A. sydowii with disease score of 2.0 as against 7.0 recorded for control. The disease score for bordeaux mixture and copper oxychloride application was 3.5 and 3 respectively. Neem cake-cowdung food base was highly favourable for multiplication and activity of fungal antagonists. The amino acids, total sugar and reducing sugar and total phenols and orthodihydroxy phenol content and activity of cellulose and chitinase were influenced by AMF colonization particularly by Is - 6 and Pi - 11. The positive change could be related with the relative disease tolerance recorded for various AMF isolates. The development of native AMF and antagonists through extensive testing in the green house and field and also the technology of AMF inoculation for established pepper vines are the first record of work.
Open Access
Effect of eyestalk ablation on androgenic gland and male secondary sexual characters in Macrobrachium Idella (Hilgendorf)
(Department of Aquaculture, College of Fisheries,Panangad, 1998) Sherine Sonia, Cubelio; KAU; Thampy, D M
The present study in the prawn, Macrobrachium idella was taken up to find out morphotypic variation among the males, the endocrine basis for these variations and also to know the role if any, the eyestalk endocrines have, on the control of androgenic gland, which in turn is controlling the development of male primary and secondary sex characters. The study conducted to find the relationship between development of sex characters with that of androgenic gland conducted using 30 male prawns of different sizes has ShO\\-l1 that these sex characters are under the control of androgenic gland and have no relationship with that of the body size. Of the 30, 22 individuals were found to have a bodylength to cheliped length ratio below 1: 1.5, their androgenic gland and appendix masculina, not fully developed indicating that they are reproductively inactive. Remaining 8 individuals have a body length to cheliped length ratio above I: 1.5. They have well developed androgenic gland and appendix masculina showing that there is positive correlation between the biomass of androgenic gland and the cheliped length. An experiment conducted to find out the effect of unilateral eyestalk ablation on growth and development of primary and seconadry sexual characters as well as on the androgenic gland, had shown that destalking brings about a positive change in the development of androgenic gland and sex characters. The increase in the size of the androgenic gland and consequent hypertrophy of primary and secondary sexual characters brought about as a result of unilateral eyestalk ablation do indicate that the GlH, a hormone produced in the eyestalk has got an inhibitory effect on androgenic gland as well as on the growth of the prawn, which when removed by " destalking" , results in the enlargement of the androgenic gland and other sexual characters.
Open Access
Effect of depuration on the meat quality of the marine clam Sunetta scripta (Linne)
(Department of Processing Technology, College of Fisheries,Panangad, 1998) Nishi Dharan, S; Damodaran Nambudiri, D
Sunetta Scripta harvested from Munabam area were subjected to depuration studies to find out whether purging had any effect on the meat quality of the clam. Result of biochemical analysis shows that the clam is a good source of various nutrients. Meat yield of clam was found to be 9.5%. Pathogenic bacteria was absent except E. coli ,. which is within the permissible limits. Depuration of clam did not significantly change the protein and glycogen content. But sand content was reduced to insignificant level within 24 hr depuration. When clams were exposed to a concentration of 109 ceUS/ml of Vibrio parahaemolyticus, there was progressive accumulation of the micro-organism and it accumulated on an average of 2.6xl05 cells/g within 6 hrs exposure. Though accumulated organism was not completely eliminated from the clams during purification in a laboratory depuration unit using U. V for sterilising the water, the number had reduced to more than 1000 fold during 48 hrs depuration. There was remarkable change in the sensory attributes and grittiness character of the clam after depuration
Open Access
Pathology of the Harderian Gland in chicken and duck
(Centre of Excellence in Pathology, College of Veterinary and Animal Sciences,Mannuthy, 1998) Mohan S; Valsala K V
The lymphoid tissue of the upper respiratory tract includes paraocular and paranasallymphoid structures as well as some lymphoid accumulations in the pharynx and larynx. However, of all these paraocular lymphoid tissue, the Harderian gland is highly immunopotent. The role of the Harderian gland in the immunopathological response was evaluated taking chicken and duck as models and RD, IBD and Duck plague vaccines as antigens. The sequential cellular response following ocular antigenic stimulation was clarified. Significant increase in the plasma cell number, enlargement of the lymphoid foci and Russell body formation following ocular antigenic stimulation were the hall marks of the immune response of the Harderian gland of the chicken but this was not so much significant in the case of ducks. Harderian gland was highly reactive especially in the chicken but not so much in the case of ducks. Intraocular vaccination was found to be comparatively more effective in immunological protection in chicken. It would therefore, appear that through local administration of the antigen at the Harderian gland the duck may not respond in the same way as in the case of chicken. It was pointed out that the local antibody produced by the Harderian gland contributed in a major way to the immunological defence at the oculonasal and oropharyngeal areas and it has an obvious relevance to the epidemiology and the control of respiratory viral diseases in the avian species.
Open Access
Bio farming in vegetables : effect of bio fertilizers in amaranth
(Department of Agronomy, College of Agriculture, Vellayani, 1998) Niranjana, N S; KAU; Meerabai, M
An experiment was conducted at the Instruction farm attached to the College of Agriculture, Vellayani, during April - Sept 1996 with objectives of finding out the impact of biofertilizers Arospirillum , AMF and dual inoculation under varying and varied levels of fertilizer on amaranthus. Study also aimed to note the economics of the integrated approach and to identify the best economic combinations which improve yield and quality of amaranth varieties Arun and Kannara local. The study had three controls and two number of replications. The results of the study revealed that yield attributing characters like no: of harvests, yield harvest", marketable yield and dry weight were highest in plots applied with dual inoculation of Azospirillum and AMF with 75 per cent dose of p.op. Azospirillum with 50 per cent POP also gave good results. Both these treatments were on par and was better than our state recommendation. Growth characters viz: Seedling growth rate, plant height, number of leaves per plant, number of branches per plant, LAI were highest. for Azospirillum inoculation at early stages. While AMF inoculation gave notable results at later stages. Physiological parameters namely Biomass, NAR, Root dry weight were significantly improved with dual inoculation and higher dose of chemical fertilizer. Leaf dry weight, RGR and CGR responded most to inoculation with Azospirillum at early stages, while at later stage AMF performed well. Kannara local was superior to 148 Arun at early stages. But Arun performed superior to Kannara local with the progress of time. Stem dry weight was significantly influenced by application of AMF. Vermicompost gave high biomass till 45 DAT. Quality of amaranth improved when biofertilizer was given with higher dose of chemical fertilizer in almost all parameters discussed. Dual inoculation with 75 per cent POP gave maximum content of total mineral and moisture. Ascorbic acid content was highest for the treatment dual inoculation with 25 per cent POP. AMF with 75 per cent POP recorded high protein content, the same bioagent with 50 per cent POP gave lowest content of oxalates. Less fiber flush of amaranth w.as got from plots given Azospirillum + 75 per cent POP. Uptake of major nutrients namely Nand P was influenced by inoculation of Azospirillum along with higher dose of chemical and AMF with lower inorganics respectively. Major nutrient status in the soil after the experiment was more in case N,P,K for dual inoculation, Arospirillum and AMF respectively. Irrespective of biofertilizer higher dose of inorganic gave greater residual amount of nutrients. Dual inoculation with 75 per cent dose and Azospirillum along with 50 per cent dose gave maximum l?:C ratio and net returns. Vermicompost recorded minimum disease score. Lower levels of chemical fertilizers recorded less infection.
Open Access
Nodulation Behaviour Of Acacia Mangium Wikd.In Response TO Rhizobium Inoculation
(Department of Tree Physiology and breeding, College of forestry,Vellanikkara, 1998) Dhaneshkumar, P; KAU; Ashokan, P K
An investigation was carried out during April 1996 to December 1997 at the College of Forestry, Kerala Agricultural University, Vellanikkara to study the nodulation characteristics of Acacia mangium Willd. plantation and to isolate and compare the efficiency of rhizobia from different agroclimatic regions of Kerala. The efficiency of these rhizobial isolates under varying conditions of soil fertility and acidity were also evaluated. Survey conducted throughout the state showed rhizobial association and nodulation in all mangium plantations. The soil of these plantations were acidic with pH values ranging from 4.9 to 6.2. Experiments conducted to study the rhizobial characteristics indicated that, the fast growers (Rhizobium spp.) as well as the slow growers tBradyrhizobium spp.) are involved in nodulation of A. mangium in Kerala. The efficiencies of different rhizobial isolates were studied in polypots using sterilized and unsterilized soil - sand mixture. Two other polypot experiments were conducted to study the response of the rhizboial isolates to liming and Nand P application. The experiments were laid out in C.R.D with three replications. Seedlings raised in unsterilized soil performed better as compared to seedlings in sterilized soil. However, the response to inoculation was more prominent in sterilized soil. In sterilized soil, seedlings inoculated with Rhizobium isolates from mangium ;MRh-3 (Mananthavadi), MRh- 2(Perambra), MRh-13 (Trivandrum), MRh-6 (Vellanikkara) and MRh-lO (Mavelikkara) and commercial mangium culture (" Agroforester - Group A") showed superiority in nodulation and growth characteristics while the isolates from A. auriculijonnis and commercial culture of A. auriculijonnis, ("Agroforester - Group C") were not much effective. Seedling - response to inoculation varied with rhizobial isolates, and mangium showed its specificity in Rhiozbium requirement for nitrogen fixation. The application of lime adversely affected nodulation capacity of the rhizobial isolates of mangium from Kerala, indicating the adaptations of these isolates to acidic soils. The nodulation efficiency of commercial culture "Agroforester - Group A" was improved by lime application, indicating that this strain is adapted to soils of higher pH. The nodulation and nitrogen fixing efficiency of the isolates were improved by supplementating fertilized N and P. The need for a starter dose of Nand P for effective nodulation and efficient Ny-fixation in mangium seedlings. MRh-6 (Vellanikkara) performed poorly in unfertilized soil, but showed superiority when applied with N and P fertilizers, indicating the sensitivity of this isolate to site conditions. As the rhizobial isolates from different agroclimatic regions of Kerala responded differently under varying soil pH and doses of Nand P, inoculation of selected strains of rhizobia depending on site conditions may be needed for successful establishment of mangium seedlings.

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