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Open Access
Survival of Xanthomonas campestris pv. Oryzae and its Control in Kuttanad
(Department of Plant Pathology, College of Agriculture, Vellayani, 1996) Mary, C A; KAU; Sasikumar Nair
The present investigation was taken up to understand the factors responsible for the recurrence of bacterial blight disease in a severe from only during the additional crop season in Kuttanad. The mode of survival of the pathogen during and in between the two major cropping seasons of Kuttanad region were also studied in detail. An extensive survey was also conducted among 115 farmers in 12 Krishibhavans of Kuttanad taluk for this purpose to collect specific informations on existing cultural practices, crop variety, nature and distribution of weed flora and self sown rice plants in and around rice fields and on wether data from June 1992 to March 1994. The efficacy of two different methods of spraying, prophylactic and curative using streptocycline, mixture of streptomycin and oxytetracycline in the proportion 1:9, Bactrinol – 100 cowdung extract on the control of bacterial blight disease was tested under field condition at Nedumudi in Kuttanad. The survey showed that there was considerable variation in the incidence of bacterial blight in Kuttanad taluk. Among the 12 Krishibhavan areas the disease incidence was maximum in Ramankari and Nedumudi and minimum in Kavalam, Kainakary and Muttar. In Neelamperoor and Thalavadi areas there was no incidence of this disease during the period of survey. Between the two major cropping seasons the disease incidence was more during the additional crop season than during Punja season. Red Triveni and Jyothy were the most popular varieties cultivated in the area and more than 50% of the farmers cultivate Red Triveni. It was observed that the variety Red Triveni as highly susceptible to bacterial blight disease. The isolate of the pathogen Xanthomonas oryzae pv. Oryzae from the rice variety Red Triveni was capable of both gelatin liquefaction and starch hydrolysis. The pathogen X. oryzae pv. oryzae was found to survive for a maximum period of 42 days in infected seed, 105 days in infected straw, 56 days in infected stubbles at room temperature, 24 days in infected stubbles under dry land condition and 14 days under wet land condition. The pathogen did not survive in soil and water. Weeds like Oryza sativa var. fatua and Paspalum conjugatum served as alternate host for the pathogen. Bacterial blight infected self sown rice plants could be seen in Kuttanad during the cropping and non cropping seasons. Due to certain specific reasons, the cultivation practices were often found to extend beyond the normal cropping seasons in the region resulting in the chances of survival of bacterial blight pathogen in the host plant itself. The specific weather conditions during the additional crop season played an important role for the severity of bacterial blight desease in this season in Kuttanad. The pathogen X. oryzae pv. oryzae was tested for sensitivity to antibiotics, Bactrinol – 100 and cowding extract under in vitro conditions. The maximum growth inhibition was obtained with oxytetracycline followed by chloramphen icol which was statistically on par with oxytetracycline. The effect of increasing concentrations of oxytetracycline in combination with streptomycin on growth of X. oryzae pv. oryzae was studied with 100, 250 and 500 ppm concentrations. The growth inhibition increased not only with the concentrations of antibiotic from 100–500 ppm but also with increasing concentration of oxytetracycline. The maximum zone of growth inhibition was obtained with 1:9 proportion of streptomycin and oxytetracycline. The five treatments selected for field evaluation trial included streptocycline at 500 ppm, streptomycin + oxytetracycline (1:9) at 250 ppm and 500 ppm, Bactrinol -100 at 500 ppm and fresh cowdung extract at 20g/1. Two different spraying methods, prophylactic and curative were evaluated in two rice varieties, T(N) 1 and jyothy. The reduction in disease index by prophylactic and curative sprayings was maximum after spraying with cowdung extract 20g/1. As regards to two methods of spraying, significant reduction in per cent disease index was obtained with curative spraying. The maximum per cent increase in grain yield over control was obtained after curative spraying with 500 ppm streptomycin and oxytetracycline mixture in jyothy followed by cowdung extract 20 g/l. In T(N) 1 and jyothy both by prophylactic and curative spraying, the thousand grain weight was maximum with cowdung extract 20 g/1. As regards to two method of spraying, significant increase in grain yield and thousand grain weight was obtained after curative spraying. In T(N) 1, both by prophylactic and curative spraying the per cent increase in straw yield was maximum with a mixture of streptomycin and oxytetracycline at 500 ppm and jyothy with cowdung extract 20g/1. In T(N)1 significant reduction in chaff per cent was recorded by prophylactic spraying while in Jyothy no significant difference was obtained by the two methods of spraying. In both these varieties the reduction in chaff per cent was maximum by spraying with cowdung extract (20g/1). It was observed that two prophylactic spraying with selected bactericidal agents, neither resulted in any significant reduction in disease index nor increase in yield as compared to curative spraying. This could be due to the fact that in Kuttanad bacterial blight disease usually occurred only around the panicle initiation stage or even later. Therefore a need based curative spraying schedule would be most effective for the control of bacterial blight disease in Kuttanad. On working out the economic benefits of controlling bacterial blight it was observed that there will be economic return only from spraying infected plants of both (T(N) 1 and jyothy with cowdung extract 20g/1. The return from plants sprayed with all other treatments in the investigation was low when compared to unsprayed control plants. Thus it will be economically advantageous to use cowdung extract to control bacterial blight of rice.
Open Access
Differential adoption of plant protection technology by farmers of Kerala - a critical analysis
(Department Of Agricultural Extension, College Of Agriculture, Vellayani, 1995) Meera, B; KAU; Thyagarajan Nair, G
Indian agriculture has undergone intensive farming with the introduction of high yielding varieties of crops. Adoption of improved technology and management practices for boosting up production of food grains have resulted in serious pest and disease complexes warranting intensive and extensive use of plant protection chemicals. A number of limitations and adverse side effects such as pest resistance to pesticides, pesticide residues, health hazards, environmental pollution and ecological imbalance have been identified recently. The components of plant protection technology were taken to study the differential adoption in important crops like paddy and vegetables by the farmers and explained the variations in their cognitive, affective and connative components of behaviour with a selected set of independent variables. The study analysed the indigenous practices of plant protection being followed by farmers and also their perception about the impact of pesticides on environmental aspects. The major constraints experienced by the farmers in the adoption of plant protection technology along with the suggestions to overcome the same were also studied. The study was undertaken in two districts of Kerala viz., Thiruvananthapuram and Alappuzha. A sample of 120 farmers each from the two districts was selected and the total sample size for the study was 240.The data were collected using an interview schedule and analysed using suitable statistical techniques. There was significant difference among the farmers of Thiruvananthapuram and Alappuzha districts with regard to their knowledge about chemical and cultural methods of plant protection. Majority of farmers of both the districts belonged to low knowledge group. The farmers of both the districts were ignorant about biological, physical and integrated methods of plant protection methods. Majority of the farmers of both the districts possessed favourable attitude towards chemical method of plant protection. The farmers of Alappuzha district were significantly higher adopters of plant protection methods than the farmers of Thiruvananthapuram district. Majority of the farmers of both the districts belonged to low perception category with regard to their perception about the utility and practicability of plant protection methods. Crops yield index and scientific orientation emerged as significant independent variables in the correlation and multiple regression analysis with regard to the farmers of both the districts. The practices, viz., controlled application of nutrients for reducing pest and disease attack in paddy and use of ‘thulsikeni’ in ‘pandals’ to trap and kill fruit flies in vegetables were judged as the most effective and scientifically rational practices adopted by farmers. The farmer’s perception about the impact of pesticides on environmental aspects was very low and majority of them belonged to low perception category. The constraint ‘untimely supply and high cost of imputs’ was the most important constraint experienced by the farmers of Thiruvananthapuram district, while ‘lack of proper drainage facilities’ was the most serious constraint experienced by the farmers of Alappuzha District. The most important suggestion given by the farmers to overcome the constraints was to ‘develop simple and more compatible plant protection technologies’ and the most important suggestion given by the experts to overcome the constraints experienced by the farmers was to ‘impart adequate training to farmers as well as extension workers on IPM practices’. The strategic model developed by integrating the salient findings of the present study, emphasized the importance of popularizing effective plant protection technology among the farmers in ecologically sensitive rice and vegetable production systems in Kerala.
Open Access
Effectiveness of soil solarization for the control of soft rot disease in ginger
(Department of Plant Pathology, College of Horticulture, Vellanikkara, 1996) Vilasini, T N; KAU; Peethambran, C K
The effectiveness of soil solarisation for the control of soft rot disease in ginger was studied at the College of Horticulture, Vellanikkara, Thrissur during March 1992 to December 1993. The beds were inoculated with Pythium aphanidermatum, five days before the solarisation. Transparent, 150 guage polyethylene sheets were used for solarizing the beds. Maximum soil temperatures recorded were 63.00, 59.00 and 46.50 at 5, 10 and 15 cm depths in solarized soil, while, that in non-solarized soils were 49.50, 43.00 and 40.00C, respectively, at 5,10 and 15 cm depths. Temperature in the solarized soil at 5 cm depth was above 50.00 C for the entire solarisation period and above 55.00 C for 38 days, while, at 10 cm depth the temperature was above 50.00 C for 35 days and above 55.00 for five days. The soil temperature at 15 cm depth never reached 50.00 C during the solarisation period. Based on the soil and air temperature recorded, two simple regression equations at 5 and 10 cm depths, one simple equation at 15 cm depth and one multiple regression equation at 10 cm depth were developed for predicting soil temperature under polyethylene mulch. Rate of germination in ginger was enhanced by solarisation. Significant effect of solarisation was observed in controlling the pre and post-emergence rotting in ginger. Increasing the period of solarisation from 30 to 45 days did not result in a corresponding reduction in the pre-emergence rotting. Trichoderma incorporated neem cake amended 30 day solarized treatment was highly effective and recorded cent percent control of the soft rot disease, while, maximum disease incidence (90.67%) was in Trichoderma incorporated neem leaves amended 45 days solarized plots. Reduction in Pythium population ranging from 79.49 to 99.1 per cent was observed in solarized plots immediately after the removal of polyethylene sheets. Solarization reduced the total fungal, bacterial, actinomycetal and Pseudomonas sp. population in the field. Plants grown in solarized plots showed better colonization of VAM and Azospirillum. Significant reduction in the nematode population was recorded by solarisation. Solarization had a profound suppressive effect on the weed population and it lasted till harvest. Solarization effect was more pronounced in dicots. Eventhough, solarisation substantially reduced weed population, its effect was less in the edges. Bulbostylis barbata, Cynodon dactylon and Cyperus rotundus survived the solarisation effect. Increased growth response of ginger plants was observed as a result of solarization. Growth parameters like height, number of leaves/plant, number of tillers, number of roots, leaf length, leaf breadth, fresh weight of shoots and rhizomes were influenced by solarisation. Significant increase in the yield was obtained through solarisation. Trichoderma incorporated and neem cake amended 30 days solarized treatment gave the maximum yield/plant (623.23 g) and also per plot yield (10159.57 g), which was 5361 per cent more than that of control. Availability of nitrogen, phosphorus and potassium was improved by solarisation. The initial cost of solarization is comparatively high, an amount of Rs. 52,500/- is required for solarizing one hectare of ginger field. An additional profit generated from this technique was Rs. 40,136/ha for 30 days solarisation.
Open Access
Nutritional status of women engaged in fishvending in Thivandurm district
(Department of Home Science, College of Agriculture, Vellayani, 1993) Karuna, M S; KAU; Prema, L
The study on the “Nutritional status of women engaged in fishvending in Trivandrum District” comprises of the socio-economic and food consumption pattern of the fisherwomen families with special reference to their actual food intake, energy expenditure and nutritional status. Families surveyed were nuclear type belonging to under privileged communities. Main occupation of the families was fishing and fishvending. Most of the families belonged to the income groups of Rs.1000 to 2000. In these families as the income increased the percentage of income spent on food items decreased. Expenditure on education and health was very low. Male members spent much money for their faulty habits like alcoholism and playing cards. Most of the families were in debts and only women were responsible for the economic management of the families. Index developed from various socio-economic variables revealed that all the families lived below the poverty line. All the families were non-vegetarians and fish was the main nonvegetarian food in their diet. However, consumption of vegetables, pulses, eggs and green leafy vegetables was less due to lack of knowledge about nutritious food. Preparation of food is not upto the standard and this results in the poor quality of the food. Unbalanced, monotonous meal pattern was observed in the community. Compared to other stages of life, special attention was given only during infancy and pre-school period. Besides fishvending women were responsible for household tasks and child care practices. The women got employment for 7 months in a year. Most of the women sold the fish either in the markets in the city or by home to home delivery. Income getting from fishvending was not in proportion to the distance covered. Time spent for fishvending activity was more than the time they spent for other activities. Most of the household activities were combined with fishvending activities. It was found that total time spent for fishvending and household activities was more than 10 hours in a day. Time spent for fishvending activities was positively associated with family income, age of the fisherwomen and income of the fisherwomen. Compared to recommended daily allowance, higher energy expenditure was noticed in all the fisherwomen studied. Majority of the women were below the normal weight. Body Mass Index of the women showed that 33.33 percent suffered from energy deficiency. Nutritional status index was developed from height, weight and body mass index. The index developed ranged from 14.04 to 18.58. A highly significant positive association was observed in between nutritional status index of the women and their height, weight, BMI, Index developed from socio-economic factors and their energy expenditure. Clinical examination of the fisherwomen revealed that 95.33 percent of the women were suffering from any one of the nutritional deficiency disorders. Consumption of food quantity and availability of nutrients from the food were not satisfactory. The nutrients like energy, calcium, iron , retinol, thiamine , niacin and riboflavin and Vitamin C were deficient in their diets. As a result nutritional deficiency symptoms were more prevalent among them. Energy expenditure was more than the intake of energy. Blood constituents like haemoglobin, total protein albumin and Vitamin A were deficient in many of the women studied. From the above findings it was concluded that the nutritional status of the fisherwomen was found to be affected by geographic, environmental and disease factors.
Open Access
Genetic divergence, prepotency and inbreeding depression in para rubber (Hevea brasiliensis Muell. Arg.)
(Department of Plant Breeding, College of Agriculture, Vellayani, 1992) Kavitha Mydin, K; KAU; Gopinathan Nair, V
A study on genetic divergence, prepotency and inbreeding depression in rubber was undertaken in an effort to identify clones for use as components of polyclonal seed gardens. Forty clones of Indian, Indonesian, Malaysian and Sri Lankan origin were evaluated in a replicated trial at the Rubber Research Institute of India. Genetic variability, correlations and the cause and effect relationships of dry rubber yield and its components were worked out. Genetic divergence was estimated employing the Mahalanobis' D2 techinique. The factors of divergence were identified through principal factor analysis. Twenty promising clones from genetically divergent clusters were subjected to seedling progeny analyses for the estimation of propotency based on performance of their open pollinated seedling progenies and inbreeding depression in the first generation of selfing. Significant clonal variation was revealed in respect of all the physiological, morphological and structural attributes studied as mean values for the fourth year of tapping for the stress period and for the peak yield period. High genetic variability for volume of latex under stress, plugging index under stress, annual mean dry rubber yield and dry rubber yield during the stress and peak periods was indicated by the high estimates of genotypic coefficient of variation. Additive gene effects offering scope for improvement through selection was indicated for dry rubber yield, latex flow rate and volume of latex during the three periods, girth increment rate, annual mean plugging index and plugging index under stress, by the moderate to high heritability estimates along with high genetic advance for these traits. Non- additive gene action was indicated by the high heritability and low genetic advance for dry rubber content during the three periods, girth and bark thickness. At both genotypic and phenotypic levels, annual mean dry rubber yield showed moderate to high positive correlations with dry rubber content and latex flow rate during the three periods, girth, girth increment rate, length of the tapping panel and bark thickness and negative correlations with yield depression under stress and plugging index during the three periods. Dry rubber yield under stress emerged as a more important component than peak dry rubber yield by its higher magnitude of positive direct effect on annual mean dry rubber yield. Latex flow rate during the stress and peak periods and annual mean volume of latex exhibited high positive direct effects on annual mean dry rubber yield while plugging index during the peak yield period, volume of latex under stress and girth increment rate had negative direct effects on annual mean dry rubber yield. The magnitude and direction of the effects of the components on dry rubber yield during the three periods varied indicating these relationships to be under different genetic control. Selection for a high dry rubber yield under stress, annual mean volume of latex and latex flow rate during the stress and peak periods and against a high plugging index during the peak period, volume of latex under stress and girth increment rate would help achieve improvement in annual mean dry rubber yield. Considerable genetic diversity was revealed by the wide range of D2 values and intra and inter cluster distances. The forty clones were grouped into eight genetically divergent clusters irrespective of their country of origin indicating the absence of any relationship between geographical diversity and genetic divergence. Volume of latex, plugging index, latex flow rate, dry rubber content and dry rubber yield contributed more towards divergence than the morphological and structural attributes. Supporting evidence was obtained from principal factor analysis which revealed the yield factor to be the main factor of divergence with respect to the clusters studied. Junveile rubber yield on test tapping, number of latex vessel rows and number of leaf flushes in seedling progenies exhibited high heritability and genetic advance indicating scope for their use as early selection parameters, while girth exhibited high heritability and low genetic advance. These three traits showed significant positive correlations with juvenile rubber yield, of which girth exhibited the strongest association. Juvenile rubber yield, number of latex vessel rows, girth and number of leaf flushes were identified as important traits for being accorded simultaneous emphasis in the computation of performance index and index scores for the determination of recovery of superior seedlings as estimates of prepotency. Nine clones were identified as likely preopotents on the basis of seedling progeny analysis at the age of two years. Selfing resulted in a lower fruit set than open pollination in the clones in general. No significant inbreeding depression was recorded for juvenile vegetative traits and rubber yield in seedlings. Clones PB 28/83, PB 215, RRII 105, AVT 73, PB 217, PB 252, Ch 26, PB 242 and PB 5/51 were identified as likely prepotents from three genetically divergent clusters. They recorded superiority for yield and various yield components. These clones exhibited synchrony in flowering and are suggested as components of a nine parent polyclonal seed garden. For a seven parent seed garden the clones suggested to be excluded are PB 5/51 and PB 242. A polyclonal seed garden comprising these nine or seven clones as components could generate good quality polycross seed material. Appropriate seed garden layouts have been suggested.
Open Access
Effect of different inputs on productivity and quality relations in njavara (Oryza sativa)
(Department of Agronomy, College of Horticulture, Vellanikkara, 1996) Meera Menon, V; Kau; Potty, N N
Productivity characteristics of Njavara (Oryza sativa), a medicinal rice variety were investigated based on the results of four separate experiments conducted at the Regional Agricultural Research Station, Pilicode, during 1994-96. The experiments were to study (i) growth and development characteristics (ii) crop weather relations (iii) response to nitrogen, phosphorus and potassic fertilizers and (iv) the effect of integrated nutrient management. Two biotypes of Njavara, the black glumed and golden yellow glumed, were tested in the first two experiments. The black glumed biotype alone was studed in the third and fourth experiments. The first experiment, consisting of the two biotypes as treatments, was conducted in wetland, open upland, heavily shaded upland and partially shaded upland, and pooled analyses of the data were conducted. Treatments of the second experiment which studied the effect of date of sowing on productivity of Njavara included 10 dates of sowing at fortnightly intervals starting from the 15th of May. Combinations of three levels of nitrogen, phosphorus and potassium, each at levels of 15, 30 and 45 kg ha-1 along with a standard and absolute control constituted the treatments of Experiment III. The last trial consisted of two treatments, one exclusively with farmyard manure and another with fertilizer alone, both at 30 kg nitrogen equivalent, two treatments with organic and inorganic manure in different ratios and three combinations of Azospirillum with 25, 50 and 75 per cent of the full farmyard manure dose. The second experiment was conducted in open upland and the third and fourth experiment in heavily shaded coconut gardens. Biometric, nutritional and quality criteria estimated through accepted methodology were used for the evaluation of treatments. An abstract of the results obtained has been presented in the following paragraphs. The results showed that three phases could be distinguished in the growth and development of Njavara. They are a primary phase of absorption and accumulation of nutrients, a grand growth phase of rapid accumulation of dry matter and a diversion phase when the seed and quality develop. Continued growth till harvest gave high yield and dry matter decline in the final phase led to superior quality characteristics. Golden yellow glumed biotype exhibited continuous growth and higher yield of grain. Black glumed biotype manifested dry matter decline in the final phase, leading to lower yield of grain and higher free amino acid content in the grain. Profound influence of cropping situation on yield and quality of grain was observed. Lowest yield of 684 kg ha-1 and highest amino acid content of 0.492 mg g-1 were observed in the uplands and highest yield of 2401 kg ha-1 and lowest amino acid content of 0.203 mg g-1 were observed when Njavara was sown in the wetlands. Viewed on the basis of variation in nutrition in the different situations, the results showed that yield limiting influences were not the deficiency of any element but the excess contents of Mg and Mn at maximum tillering and of P, K, S, Zn and Cu at panicle initiation stages in the plant. This appeared to be a highly significant result in the context of stagnant productivity of crops like rice. Content of free amino acids in the grain appeared to be unique characteristic of Njavara rice. Among the amino acids, sulphur containing amino acids, methionine and cysteine were also present. Possibly, these amino acids are related to the medicinal value of Njavara in the treatment of rheumatic complaints, the symptoms of which resemble thiamin deficiency. Inherently high yield potential of Njavara was expressed in the date of sowing trial. Both the biotypes recorded grain yields of 6000 kg ha-1 when sown on most favourable dates. Weather influence contributed to be highest yield through two ways, firstly, by increasing the total dry matter yield and secondly, by improving the grain-straw ratio. The golden yellow glumed biotype produced higher grain yield than the black glumed biotype. Application of N, P and K fertilizers increased the uptake of all the elements studied. Positive interaction of fertilizer elements on content and uptake of elements was also evident. However, application of fertilizers could influence only the straw yield significantly. One possible reason for the absence of significant improvement of grain yield by fertilizer application appeared to be due to the high levels of application. Even 15 kg ha-1 of N, P and K were found to be excess in shaded situations. Results of the integrated nutrient management studies showed that level of application was more important than the source in affecting the ultimate yield. Farmyard manaure application led to a more balanced development of the components of yield whereas fertilizer source improved only the floret number per panicle. Nutritional management at levels below 15 kg ha-1 N equivalent significantly reduced the yield. Azospirillum did not have any effect in increasing the yield of Njavara. The results of the different experiment brought out the role of soil and atmosphere environment as well as the biotype influence on the yield and contributed to the development of the concept of soil-plant-atmospheric continuum on the technical side. On the pracical side they showed that Njavara is an ideal crop for growing in the first crop season in the uplands in North Malabar when no other crop can be grown and that growing in heavily shaded uplands will give high quality grain. Advantageously, the crop will not require intense management.
Open Access
Genetic analysis of biological nitrogen fixation traits and yield components in cowpea (Vigna unguiculata (Linn). walp)
(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 1995) Sreekumar, K; KAU; Manikantan Nair, P
A study on the parameters of variability, correlations, path-coefficients, combining ability, gene action and heterosis in cowpea was undertaken at the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 1991 to 1993. Fifty three genotypes of cowpea collected from different sources were planted in a field experiment for the estimation of variability, correlation and path coefficient. Eleven biological nitrogen fixation characters, viz., number of days to flower, length of primary root, number of secondary roots, number of nodules in the primary root, number of nodules in the secondary roots, total number of nodules, weight of effective nodules in the primary root, weight of nodules in the secondary roots, total weight of nodules, nitrogen content in the plant at 50 per cent flowering and plant dry weight and six yield characters, viz., grain yield per plant, length of pods, number of pods per plant, number of seeds per pod, weight of 100 seeds and seed protein content were considered for this study. The ten selected varieties/types from the initial evaluation trial were crossed in a line x tester model, keeping the three high nitrogen fixing types and three high yielding types as lines (total six lines) and two low nitrogen fixing and two low yielding types as testers (total four testers). The F1’s along with their parents were compared in a field experiment and combining ability, gene action and heterosis were estimated. The study of combining ability and gene action were confined to six biological nitrogen fixation characters, viz., number of days to 50 per cent flowering, weight of nodules in the primary root, total weight of nodules, weight of effective nodules, dry weight of the plant and nitrogen content per plant and six yield characters, viz., length of pod, number of seeds per pod, number of pods per plant, hundred seed weight, seed protein content and grain yield per plant. The analysis of variance revealed that a considerable amount of variation among the varieties was present with respect to the characters under study. Characters like number of days to flower, total nodule weight, nitrogen content per plant, weight of 100 seeds and seed protein content had recorded narrow relative magnitude of difference of phenotypic and genotypic coefficient of variation along with high heritability estimate. Moderate magnitude of difference of PCV and GCV along with moderate heritability was recorded for the characters viz., number of nodules in the primary root, number of nodules in the secondary roots, total number of nodules, weight of effective nodules in the primary root, weight nodules in the secondary root, length of pods and number of pods per plant. Plant dry weight and grain yield registered a wider difference of PCV and GCV along with low heritability indication the greater influence of environment over these two characters. Genetic advance as percentage of mean was found to be high for the characters like number of nodules in the secondary roots, weight of effective nodules in the primary root, weight of nodules in the secondary roots, total nodule weight, number of pods per plant and 100 seed weight and moderate for number of nodules in the primary root, total number of nodules and grain yield. Low genetic advance was recorded by number of days to flower, plant dry weight, nitrogen content per plant, length of pod and seed protein content. Hence characters such as number and weight of nodules in the primary root, number and weight of nodules in the secondary roots, total number and weight of nodules, number of pods per plant and weight of 100 seeds may be controlled by additive genes whereas days to 50 per cent flowering, nitrogen content in plant, length of pod and seed protein content may be controlled by non-additive genes. Correlation coefficients were workedout at the genotypic and phenotypic levels. Based on the genetic correlation of characters studied, it was understood that high nitrogen fixing genotypes may not be high yielders because of the antagonistic relationship between grain yield and total nitrogen per plant. Weight of nodules in the primary root and total nodule weight were positively correlated with the nitrogen content in plant. Hence genotypes which was able to form effective large nodules on the primary root system seems to be a better nitrogen fixer. Number of days to 50 per cent flowering had negative genotypic correlation with grain yield. Hence an early flowering genotype may be better yielder than a late flowering type. Weight of hundred seeds and seed protein content exhibited very strong negative correlation indicating that small seeded genotypes may be better with respect to protein content. Grain yield recorded positive phenotypic and genotypic correlation with number of pods per plant. Path coefficient analysis at the genotypic level revealed that total number of nodules had the highest positive direct effect on nitrogen content per plant followed by weight of effective nodules in the primary root and weight of effective nodules in the secondary root. Highest positive direct effect was recorded for length of pod with grain yield. The combining ability analysis revealed that both additive and non-additive gene actions were important for all the characters under study. However GCA and SCA variance ratio which was less than unity for all the traits under study indicated the predominance of non-additive gene action in the inheritance of these traits. Considering the combining ability effects, VCP 4 was found to be the best general combiner for most of the biological nitrogen fixing characters and V 322 was the best general combiner for the grain yield. The cross combination of V 27 x C 152 and V 271 x Co Vu 85020 showed the best performance with respect to sea for the character number of days to 50 per cent flowering while VCP 4 x C 152 for weight of nodules in the primary root and nitrogen content in plant. DPLC 210 x PTB 2 recorded high sea for total weight of nodules, weight of effective nodules, dry weight of the plant and number of pods per plant, on the other hand Co Vu 358 x C 190 recorded high sca for length of pod, number of seeds per pod and seed protein content. The cross combination V 322 x C 190 exhibited high sca for hundred seed weight and V 27 X C 152 for grain yield per plant. Marked heterosis was observed in many cross combinations for most of the characters studied and pronounced heterotic expression was obtained for weight of nodules in the primary root, total weight of nodules, weight of effective nodules, dry weight of the plant at 50 per cent flowering, number of pods per plant and grain yield. It was already established that these characters are predominantly governed by the non – additive gene action. Hence the heterotic vigour expressed by the hybrid combination with respect to these characters are justified. Since the biological feasibility for the exploitation of heterosis is not economical as a plant improvement programme in this crop, genetic improvement of these trait can be brought about more effectively through combination breeding involving genetically diverse and high combining parants.
Open Access
Incorporation of resistance to fruit cracking in a bacterial wilt resistant genetic background in tomato
(Department of Olericulture, College of Horticulture, Vellanikkara, 1995) Sadhan Kumar, P G; KAU; Rajan, S
An investigation on “Incorporation of resistance to fruit cracking in a bacterial wilt resistant genetic background in tomato” was undertaken in the Department of Olericulture, College of Horticulture, Vellanikkara during the period from January, 1991 to March, 1994. The findings are succinctly mentioned below. Evaluation for bacterial wilt resistance revealed that Sakthi and LE 79 – 5 are consistently resistant to bacterial wilt. Four addition sources of bacterial wilt resistance were identified viz., LE 214, CAV – 5, LE 415 and LE 382 – 1. Resistances to bacterial wilt in these lines was governed by recessive genes. Screening for resistances to fruit cracking resulted in the identification of fifteen tomato genotypes which were found to be resistant to both radial and concentric cracking. Resistances to concentric fruit cracking in these lines were found to be dominant. All the bacterial wilt resistant genotypes had a higher content of total phenols, O.D. phenol and ascorbic acid than the susceptible line pusa Ruby. The crack resistant varieties had a higher content of insoluble solids and pectin, lower content of acidity, total sugar and reducing sugar in fruits, thick fruit skin and pericarb as compared to susceptible variety. The elasticity of skin was also higher in crack resistant genotypes. Crack resistant varieties had a compact arrangement of parenchymatous cells when compared with crack susceptible variety. The resistant lines had a thicker cuticle also. The F1 S developed by line x tester crossing were susceptible to bacterial wilt. All the same, they were resistant to both radial and concentric fruit cracking indicating dominant gene action for crack resistance. The F2 segregants with combined resistance to both bacterial wilt and fruit cracking were selected for further improvement.
Open Access
Characterisation and evaluation of the dwarf cattle of Kerala
(Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences, Mannuthy, 1994) Girija, C R; KAU; Sosamma, Iype
The native cattle of Kerala have been treated as non descript animals always eventhough they possess some special features. The dwarf cattle often called as Vechur were very popular in Central Travancore until 35 years back. With the emergence of the crossbred population of cattle the traditionally reared local cattle have gradually suffered genetic erosion. Under this circumstance, the present work was undertaken to characterize and evaluate the germplasm of local dwarf cattle of Kerala by studying (a) the karyotype and morphology of chromosomes using G-banding (b) the population structure by means of gene frequencies of different blood proteins (c) the growth and production performance. The characterization and the evaluation would help in finding out the genetic differences of the dwarf cattle which will help in deciding about the conservation of their germplasm as a reserve for the future. The dwarf cattle maintained under the ICAR scheme on “Conservation of germplasm of Vechur cattle of the coastal area and the dwarf cattle of the high ranges of Kerala” formed the material for the study. The characterization and evaluation was carried out through the cytogenetic, immunogenetic and polymorphism studies as well as through the description of the growth and production traits. Karyotype analysis was carried out using peripheral blood leukocyte culture technique described by Halnan (1977) and Halnan (1989) with suitable modifications. G-banding of chromosomes were done by the method described by Thiagarajan (1993). Blood protein polymorphism systems such as Haemoglobin and transferring were studied by poly acrylamide gel electrophoresis in horizontal dimension. (Gahne et. al. 1977) with suitable modifications. The statistical analysis of the growth and production data were done as suggested by Snedecor and cochran (1967). The diploid chromosome number of the dwarf cattle was found to be 60, with 29 pairs of autosomes and one pair of sex chromosomes. All the autosomes and the ‘Y’ chrosome were acrocentric. The X chromosome was submetacentric. The relative length of the autosomes ranged from 1.757 to 5.431 per cent. The relative length of the X and Y chromosomes were found to be 5.591 per cent and 2.875 per cent respectively. In the karyological array, the X chromosome occupied the first position. The X chromosome was biarmed and the arm ratio and centromere index obtained were 2.182 and 0.314 respectively. The karyotype and morphometric measurements resembled the finding in Bos indicus group of cattle. The G-banding pattern of chromosomes revealed 72 regions and 314 G-bands. The Y chromosome had 7 G-bands in the ‘q’ arm which resembled the ‘q’ arm of Bos taurus described in the international system for cytogenetic nomenclature of domestic animals. There were two haemoglobin variants HbA and HbB and three phenotypes viz. HbAA, ,HbAB and HbBB , in the population. The heterozygocity was found to be 0.4815. The population was found to be in genetic equilibrium with respect to the Haemoglobin locus. Six transferring phenotypes controlled by three alleles TfA, TfD and TfE were observed. The frequency of TfE (0.359) allele in the dwarf cattle was as high as the frequency of the allele reported in the zebu cattle. The absence of transferring variants like TfF, TfH, TfN and TfG and higher frequency of TfE allele are probably indicative of the genetic isolation of the population from exotic breeds. The absence of TfB and TfF allele which is present in Gir, Hariana, Kankrej, Kangayam, Ongole, Red Sindhi, Sahiwal and Tharparkar also indicates that the dwarf cattle has not inherited genes from the above cattle breeds. The body weights and measurements of calves at birth studied showed that the male calves had a higher body weight (12.55 ± 0.31 kg with a CV of 7.86 per cent) than female calves (10.78 ± 0.40 kg with a CV of 15.02 per cent). The same trend was observed with regard to the birth body measurements also. The heart girth measurement and body weight showed a positive correlation from birth to the 24th fortnight. There is a 100 per cent increase in the birth weight by the 5th fortnight and a three-fold increase by the 10th fortnight. The average daily gain in weight for the four periods I e., fortnights 0-6, 7-12, 13-18 and 19-24 were 0.160 ± 0.011, 0.167 ± 0.018, 0.212 ± 0.011 and 0.139 ± 0.015 respectively for female calves and 0.188 ± 0.023, 0.145 ± 0.016, 0.116 ± 0.025, 0.242 ± 0.049 kg respectively in male calves. During the period from birth to 6th fortnight the growth rates in males and females were similar. The gain in body weight per day during the periods from 7 to 12th and 13 to 18th fortnight was comparatively less for males but the trend reversed during the period of fortnights for 19 to 24th. The average body weights of adult females and males were 126.90 ± 3.56 kg (CV 16.39%) and 210 ± 15.75 kg (CV 14.95%) respectively. The body measurements such as length, heart girth and height (in cms) in females were 97.5 ± 1.12 (CV 5.85%), 115.60 ± 1.32 (CV 5.82%) and 87.53 ± 0.82 (CV 4.82%) respectively. The corresponding figures in males were 111.5 ± 3.77 (CV 6.76%), 146.0 ± 2.92 (CV 3.99%) and 107.5 ± 1.35 (CV 2.50%) respectively. The average body weights and measurements were lesser than those reported in other Indian breeds and crossbred cattle. The total lactation milk production performance of the dwarf cattle was 471.68 ± 38.72 kg (CV 45.29%) in an average lactation length of 217 ± 16.50 days (CV 32.20%). The average daily yield was 2.17 ± 0.11 kg (CV 29.48%). The dwarf cattle attained a peak yield of 3.71 ± 0.16 kg (CV 21.5%) in 23.23 ± 1.703 days (CV 37.38%). The milk production performance eventhough was lesser than crossbreds or some recognized Indian breeds, the milk production in comparison with the body size was reasonable. Considering the morphology of the Y chromosome, the Hb as well as Tf polymorphism and their allelic frequencies, it is to be summarized that the stock of dwarf cattle of Kerala maintained at Kerala Agricultural University is genetically isolated from the other cattle breeds of the country and world. The body size and milk production of the cow indicates its suitability for a farmer who requires milk just for home consumption. The study strongly confirms the necessity of conservation of the dwarf cattle of Kerala which is the smallest variety available in India and perhaps in the world itself.

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