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ThesisItem Open Access Fungal diseases of selected medicinal plants of Kerala(Department of Plant Pathology, College of Horticulture, Vellanikkara, 1991) Sukumara, Varma A; KAU; ; Abi, CheeranThesisItem Open Access Qualitative changes of yoghurt prepared from milk preserved by different methods(Department of Dairy Science, College of Veterinary and Animal Sciences,Mannuthy, 1990) Prasad, V; KAU; Sukumaran, M VAn experiment was conducted to study the qualitative changes of yoghurt prepared from milk preserved by different methods. An attempt was also made to study the efficiency of LP system in controlling psychrotrops in refrigerated milk thereby extending the keeping quality of such stored milk. The efficacy of the system was compared with pasteurized milk and raw milk stored under similar conditions. An exhaustive review of literature has been presented on the use of various preservation methods employed in milk, quality of yoghurt and other related aspects. The methods of analyses of some important components of milk and yoghurt has been detailed. The milk after collection were divided into three portions and used for various treatments A (raw milk as such), B (laboratory pasteurized) and C )LP activated). Samples in each treatment were again divided into four parts and stored under refrigeration for 0, 24, 48 and 72 hours for further analysis and yoghurt preparation. When raw milk was stored under refrigeration, acidity developed to a significantly high level (P < 0.05) at the end of 72 hours of storage whereas no significant increase in activity was noticed in treatments B and C. This result was corroborated by a decrease in both psychrotrophic and total bacterial counts in milk under treatments B and C. So it was concluded that the development of acidity in treatment A could be due to bultiplication of microbes particularly psychrotrophs. In B and C its growth was arrested by the treatments. Hydrogen peroxide and thiocyanate were detected in all raw milk samples at a level of 3.78 and 7.33 ppm respectively. The level of H2 O2 and SCN – used for the activation of LP system for the study were found to be adequate, since the psychrotrophic count in milk at the end of 72 hours in treatment C was less than that found in treatment B. The residual H2 O2 and SCN – contents at the end of 72 hours were found to be within the normal level found in milk thus having no health hazards, by this method of preservation. When yoghurt samples were prepared from stored milk no significant differences between treatments were noticed in the values of fermentation period, acidity, pH, total proteins and total solids. A significant increase in NPN and tyrosine values were noticed in yoghurt prepared from milk under treatment A indicating extensive proteolysis in milk during storage under this treatment. However, no difference was noticed between treatments B and C. Diacetyl and acetaldehyde were produced at a desirable level in yoghurt samples under all the three treatments. A proper ratio of Str. Thermophiles – 6 and L. bulgaricus – 4 was found to be maintained in all yoghurt samples. On organoleptic evaluation the yoghurt prepared from milk under treatment C could not be distinguished from other two treatments. In fact, yoghurt under C got a higher total score on organoleptic evaluation than A and B. Low score for body and texture, and falvour was observed under treatment A. This may be attributed to the effect of growth of psychrotrophs in raw milk during refrigeration. From the results, it was concluded that good quality yoghurt can be prepared from milk stored under refrigeration following pasteurization or LP activation. Yoghurt samples prepared from milk stored under treatment A was found to be of inferior quality when compared to B and C. The result also confirmed that LP system can be recommended as a safe preservative for extending storage life of refrigerated milk. When such stored milk was used for yoghurt production, no significant difference in the quality was noticed when compared to yoghurt under treatment B. Suggesting that LP activated milk can be conveniently and economically used for the production of fermented milk products like yoghurt without any apparent harmful effect.ThesisItem Open Access Survival of Xanthomonas campestris pv. Oryzae and its Control in Kuttanad(Department of Plant Pathology, College of Agriculture, Vellayani, 1996) Mary, C A; KAU; Sasikumar NairThe present investigation was taken up to understand the factors responsible for the recurrence of bacterial blight disease in a severe from only during the additional crop season in Kuttanad. The mode of survival of the pathogen during and in between the two major cropping seasons of Kuttanad region were also studied in detail. An extensive survey was also conducted among 115 farmers in 12 Krishibhavans of Kuttanad taluk for this purpose to collect specific informations on existing cultural practices, crop variety, nature and distribution of weed flora and self sown rice plants in and around rice fields and on wether data from June 1992 to March 1994. The efficacy of two different methods of spraying, prophylactic and curative using streptocycline, mixture of streptomycin and oxytetracycline in the proportion 1:9, Bactrinol – 100 cowdung extract on the control of bacterial blight disease was tested under field condition at Nedumudi in Kuttanad. The survey showed that there was considerable variation in the incidence of bacterial blight in Kuttanad taluk. Among the 12 Krishibhavan areas the disease incidence was maximum in Ramankari and Nedumudi and minimum in Kavalam, Kainakary and Muttar. In Neelamperoor and Thalavadi areas there was no incidence of this disease during the period of survey. Between the two major cropping seasons the disease incidence was more during the additional crop season than during Punja season. Red Triveni and Jyothy were the most popular varieties cultivated in the area and more than 50% of the farmers cultivate Red Triveni. It was observed that the variety Red Triveni as highly susceptible to bacterial blight disease. The isolate of the pathogen Xanthomonas oryzae pv. Oryzae from the rice variety Red Triveni was capable of both gelatin liquefaction and starch hydrolysis. The pathogen X. oryzae pv. oryzae was found to survive for a maximum period of 42 days in infected seed, 105 days in infected straw, 56 days in infected stubbles at room temperature, 24 days in infected stubbles under dry land condition and 14 days under wet land condition. The pathogen did not survive in soil and water. Weeds like Oryza sativa var. fatua and Paspalum conjugatum served as alternate host for the pathogen. Bacterial blight infected self sown rice plants could be seen in Kuttanad during the cropping and non cropping seasons. Due to certain specific reasons, the cultivation practices were often found to extend beyond the normal cropping seasons in the region resulting in the chances of survival of bacterial blight pathogen in the host plant itself. The specific weather conditions during the additional crop season played an important role for the severity of bacterial blight desease in this season in Kuttanad. The pathogen X. oryzae pv. oryzae was tested for sensitivity to antibiotics, Bactrinol – 100 and cowding extract under in vitro conditions. The maximum growth inhibition was obtained with oxytetracycline followed by chloramphen icol which was statistically on par with oxytetracycline. The effect of increasing concentrations of oxytetracycline in combination with streptomycin on growth of X. oryzae pv. oryzae was studied with 100, 250 and 500 ppm concentrations. The growth inhibition increased not only with the concentrations of antibiotic from 100–500 ppm but also with increasing concentration of oxytetracycline. The maximum zone of growth inhibition was obtained with 1:9 proportion of streptomycin and oxytetracycline. The five treatments selected for field evaluation trial included streptocycline at 500 ppm, streptomycin + oxytetracycline (1:9) at 250 ppm and 500 ppm, Bactrinol -100 at 500 ppm and fresh cowdung extract at 20g/1. Two different spraying methods, prophylactic and curative were evaluated in two rice varieties, T(N) 1 and jyothy. The reduction in disease index by prophylactic and curative sprayings was maximum after spraying with cowdung extract 20g/1. As regards to two methods of spraying, significant reduction in per cent disease index was obtained with curative spraying. The maximum per cent increase in grain yield over control was obtained after curative spraying with 500 ppm streptomycin and oxytetracycline mixture in jyothy followed by cowdung extract 20 g/l. In T(N) 1 and jyothy both by prophylactic and curative spraying, the thousand grain weight was maximum with cowdung extract 20 g/1. As regards to two method of spraying, significant increase in grain yield and thousand grain weight was obtained after curative spraying. In T(N) 1, both by prophylactic and curative spraying the per cent increase in straw yield was maximum with a mixture of streptomycin and oxytetracycline at 500 ppm and jyothy with cowdung extract 20g/1. In T(N)1 significant reduction in chaff per cent was recorded by prophylactic spraying while in Jyothy no significant difference was obtained by the two methods of spraying. In both these varieties the reduction in chaff per cent was maximum by spraying with cowdung extract (20g/1). It was observed that two prophylactic spraying with selected bactericidal agents, neither resulted in any significant reduction in disease index nor increase in yield as compared to curative spraying. This could be due to the fact that in Kuttanad bacterial blight disease usually occurred only around the panicle initiation stage or even later. Therefore a need based curative spraying schedule would be most effective for the control of bacterial blight disease in Kuttanad. On working out the economic benefits of controlling bacterial blight it was observed that there will be economic return only from spraying infected plants of both (T(N) 1 and jyothy with cowdung extract 20g/1. The return from plants sprayed with all other treatments in the investigation was low when compared to unsprayed control plants. Thus it will be economically advantageous to use cowdung extract to control bacterial blight of rice.ThesisItem Open Access Land evaluation and suitability rating of the major soils of Onattukara region(Department of soil science and agricultural chemistry, College of Agriculture, Vellayani, 1998) Premachandran, P N; KAU; Subramonia Iyer, MThe need for a scientific approach in inventorying and utilizing land resources most economically and efficiently is now universally accepted. Agricultural prosperity, to a great extent, depends on judicious use of soils and rational application of soils data. A reconnaissance soil survey of Onattukara region was undertaken to study the extent and distribution of the different soils for soil classification, land capability and irrigability classification and for subsequent land evaluation. The study reveals that Onattukara region extends over an area of 40,948 ha. The soil map showing the distribution of the identified twenty soil series has been prepared. The climatological data reveals that the soil moisture regime is , ustic and the soil temperature regime is isohyperthermic. The soils have been classified as per Soil Taxonomy (U.S. Soil Survey Staff, 1975) and Keys to Soil Taxonomy( U.S. Soil Survey Staff, 1994 and 1996). Ten series were classified under Entisols, SIX under Inceptisols, three under Ultisols and one under Alfisols. Based on the inherent soil characteristics and landscape features, the soils have been grouped into three land capability classes, nine capability subclasses and land capability map prepared. The soils were classified into five land irrigability classes, eight land irrigability subclasses and land irrigability map prepared. The soils were evaluated based on principles of land evaluation and rating of productivity parameters. The productivity rating of the soil propertIes agamst the productivity index for nee, coconut, sesamum ,cassava and banana were made and conclusions arrived at. Productivity rating shows that Kottakakam series is best suited for rice, Palamel, best for coconut and banana, Artuva and Mahadevikad, for sesamum and Palamel and Vallikunnam, for cassava. On the basis of the studies carried out, a land use is proposed for the twenty soil series of Onattukara region based on crop suitability ratings and crop suitability map prepared for the five crops taken for study. A proper soil survey interpretation provides information on soil potential, productivity and limitations in their sustained use. Soil survey information forms the major basis for land evaluation. A thorough knowledge of the potentialities and limitations of every piece of land is a prerequisite in its efficient utilization. A systematic survey is essential for the evaluation and classification of the soils based on their inherent soil characteristics, land capability, land irrigability and land suitability. A systematic survey and evaluation of the soils of Onattukara region was taken up to study, interpret, classify and to show their location and extent on base maps. It is hoped that the present study would open up avenues for further investigations on land evaluation, crop suitability and other management aspects for sustained use of soil resource data to the best advantage. This will also form the basis for microlevel planning for integrated and sustainable development of the region under Panchayat raj.ThesisItem Open Access Management of foot rot of black pepper (piper nigrum L.) with va mycorrhiza and antagonists(Department of Plant Pathology, College of Agriculture, Vellayani, 1998) Christin Robert, P; KAU; Sivaprasad, PExtensive investigation was carried out to develop a native microbial inoculant based technology involving Arbuscular Mycorrhizal Fungi (AMF) and fungal antagonists for the foot rot disease management and growth improvement of black pepper in the nursery and field. Phytophthora capsici Leonian emend A. Alizadeh and P.H. Tsao, the foot rot pathogen isolated from Peringammala, Thiruvananthapuram district was found most virulent isolate. Seven native AMF cultures and fifty fungal antagonists were isolated from Kerala soils. AMF isolates were screened in the green house for plant growth improvement and disease tolerance in comparison with identified species-Glomus fasciculatum, G.clarum and Gigaspora margarita. Of the ten AMF tested isolates Is - 6, Pi - 11, Pi - 9, G. fasciculatum and Gigaspora margarita were very effective in stimulating growth and nutrient (P, K, Ca, Mg, Cu, Fe, Mn and Zn) uptake of black pepper. Regarding the ability of AMF in reducing the foot rot incidence, Glomus fasciculatum recorded the lowest plant mortality and root rot index (53.35% and 62.50%) followed by Is - 6 (60.00% and 64.77%) and Pi - 11 (60.64% and 68.18%) as against 100 per cent mortality and 98.60 per cent root rot index noticed in control. The above five cultures were subjected for further studies. Characterisation of AMF associated with different genotypes of black pepper grown in various soil types indicated the definite influence of soil type on AMF colonization. Sandy soil (oxyaquic quartpsamment) harboured maximum root colonization while forest soil (haplic argiustoll) had the lowest. Species of Glomus particularly G. fasciculatum was the predominant AMF associated with black pepper irrespective of soil type. As an exception Acaulospora and Gigaspora species were frequently noticed in sandy soils. Based on the ability of the fungal antagonists to suppress P. capsici in vitro either through mycoparasitism, antibiosis or soil fungistasis, 24 isolates were selected for green house studies. In the further testing isolates A1, A13, A21, A22and A35 significantly reduced the foot rot infection and increased the plant growth. They showed better population build up in the soil and suppressed the P. capsici population considerably. These native antagonists were further tested in combination with selected AMF in the green house and field. Under green house condition, combination of G. fasciculatum x A1 or A21 showed significant influence on growth stimulation, while Is - 6 x A22 recorded lowest mortality of 32.90 per cent due to foot rot incidence as against 97 per cent in control. The dual inoculation of Is - 6 x A21 and Pi - 11 x A1 was highly effective in plant growth stimulation and disease suppression. Both the combination recorded less than 60 per cent infection and mortality due to the disease, while control showed 95.66 per cent infection and plant mortality. Bordeaux mixture and copper oxychloride recorded 66.67 and 59.68 per cent mortality respectively. AMF colonization and multiplication of antagonists were also favoured by dual inoculation. The potential AMF isolates Is - 6 and Pi - 11 were identified as species of Glomus while, the antagonistic isolates A1, A13, A21, A22, and A35 were confirmed as aspergillus fumigatus Fres., Fusarium oxysporum Schlecht. Ex Fr. Aspergillus sydowii (Bain. & Sart.) Thom. & Church, Trichoderma viride Pers. Ex Gray. And Gliomastix murorum (Corda) Hughes respectively. A technique for AMF inoculation to established pepper vines was developed using ‘carrier plants’. Raising sorghum with AMF inoculation around the pepper vines was found effective to achieve intense colonization in pepper roots by the introduced AMF in the field. This technique developed for the pepper vines may be tried for extending to other perennial crops for AMF inoculation. Promising AMF cultures Pi - 11, Is - 6, G. fasciculatum and antagonists Aspergillus fumigatus, A. sydowii, Trichoderma viride were further tested on eight year old established pepper vines following ‘carrier plant’ based AMF inoculation and cowdung - neem cake based antagonist inoculation. The treatment Pi - 11 x A. Sydowii was most effective with no symptom development, followed by Is- 6 x T. Viride or A. sydowii with disease score of 2.0 as against 7.0 recorded for control. The disease score for bordeaux mixture and copper oxychloride application was 3.5 and 3 respectively. Neem cake-cowdung food base was highly favourable for multiplication and activity of fungal antagonists. The amino acids, total sugar and reducing sugar and total phenols and orthodihydroxy phenol content and activity of cellulose and chitinase were influenced by AMF colonization particularly by Is - 6 and Pi - 11. The positive change could be related with the relative disease tolerance recorded for various AMF isolates. The development of native AMF and antagonists through extensive testing in the green house and field and also the technology of AMF inoculation for established pepper vines are the first record of work.ThesisItem Open Access Optimization of shade, nutrients and growth regulators for cut-flower production in anthurium(Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 1997) Salvy, B R; KAU; Valsalakumari, P KExperiments were conducted at the Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, during 1995-1997 to optimize the shade, nutrients and growth regulators for cut flower production in Anthurium andreanum var. 'Hawaiian Red'. The effects of three growth regulators, viz., GA, BA and Ethephon with two concentration each, 750 ppm and 1500 ppm, in combination with four nutrients, viz., 1 % fertilizer complex, Hoagland solution, Knop's solution and Ohio solution, were assessed at four levels of shade, viz., 80, 70, 60 and 50 per cent. Post harvest studies were also carried out with an objective to prolong the longevity of cut blooms, following different stage of harvest and giving different pulsing and hold- ing treatments. Results revealed that the different levels of shade and growth regulators significantly influenced all the morphological characters of the plant, viz., height, spread, number of leaves, leaf area, petiole length, number of branches and suckers. Plant height was significantly superior under 80 per cent shade (5.68 cm), 1 per cent fertilizer complex sprays (5.46 cm) and BA 750 ppm sprays (6.11 cm), were superior individually. The superior treatment combination was 70 per cent shade + Knop's solution + 750 ppm BA (7.60 cm). The maximum plant spread (EW - 20.13 cm and NS - 19.80 cm) was under 80 per cent shade. Eighty per cent shade + Ohio solution + 1500 ppm BA recorded the maximum EW spread (26 cm), while NS spread was maximum (28.70 cm) in the treatment combination involving 80 per cent shade + 1 per cent fertilizer complex + 750 ppm BA. The best values of growth parameters were recorded under 80 per cent shade and 750 ppm BA, viz., 14.03 and 14.02, respectively, for number of leaves (9.29 and 10.79 cm, respectively) for petiole length, leaf length (7.76 and 8.33 cm, respectively), leaf breadth (5.93 cm and 6.49 cm, respectively), index leaf area (50.75 cm- and 59.60 cm-, respectively). Total leaf area (423.25 cm? and 517.56 cm>, respectively) and LAI (0.43 and 0.51, respectively) were maximum under 70 per cent shade and BA 750 ppm. The superior combinations for these parameters were 80 per cent shade + Ohio solution + 750 ppm BA, for highest number of leaves (26.20); 80 per cent shade + Ohio solution + 1500 ppm BA for highest petiole length (14.00 cm); 60 per cent shade + Hoagland solution + 750 ppm BA for maximum leaf length (10.50 cm) and 60 per cent shade + 1 per cent fertilizer complex + 750 ppm BA for maximum leaf breadth (8.0 cm). The treatment combination involving 70 per cent shade + 1500 ppm BA with Hoagland solution was the best for maximum index leaf area (90.90 cm-) while same with 1 per cent fertilizer complex was the best for total leaf area (872.00 cm-) and LAl (0.89). Branching started only after 6 months from the commencement of the experiment. It was one month earlier under 80 per cent and 70 per cent shade levels. Significantly superior branching was observed under 80 per cent shade (2.29), fertilizer complex (2.13) and GA 750 ppm (2.37), individually. Treatment combination involving 80 per cent shade + 1 per cent fertilizer complex + 1500 ppm GA recorded the maximum branching (3.80). .' . Sucker production was the best under 80 per cent shade (1.35) and 750 ppm BA (1.35). Treatment combination involving 80 per cent shade + Ohio solution + 750 ppm BA produced the maximum number of suckers (2.5) per plant. Growth behaviour with respect to height, number of leaves, leaf area, number of suckers and branches showed a linear trend. The linear growth rate was consistent and positive under the treatment combination of 80 per cent shade + Ohio solution + 750 ppm BA. Dry matter production was significantly superior under higher shade intensity and lower concentration of BA. The dry weight ratio of shoot:root was the highest (2.15) under the treatment combination of 80 per cent shade + Knop's solu- tion + 750 ppm Ethephon. Leaf longevity was significantly highest (161 days) in the treatment combination, 70 per cent shade + 1 per cent fertilizer complex + 750 ppm BA. Stomatal distribution and size were not influenced significantly by the treatments. Leaf senescence, though was influenced significantly, no particular trend was observed. The aerial root production decreased significantly with decrease in shade intensity. Primary and secondary roots and root spread were the highest under 80 per cent shade and BA 1500 ppm. Maximum root length was observed under 60 per cent shade. Flowering with small stalkless flowers was first observed under 50 per cent shade with GA 750 ppm, at the age of 8 months. Inflorescence with normal size were produced at the age of 13 months, first under 80 per cent shade followed by 70 per cent, 60 per cent and 50 per cent. The time taken for various stages of flower development and the number of inflorescence produced per plant were not influenced significantly. Chlorophyll content in leaf was significantly influenced by shade levels. There was a decrease in chlorophyll content with decrease in intensity of shade. Eighty per cent shade and 1500 ppm Ethephon was superior with respect to the content of green pigments. The highest total chlorophyll content was observed in the treatment combination involving 60 per cent shade + Ohio solution + 750 ppm Ethephon (8.8 mg g -1 fresh weight). Anthocyanin content was not influenced by any of the treatments or combinations. The leaf nutrient content and uptake of nutrients was maximum under 80 per cent shade and BA 750 ppm, individually. Post harvest studies showed that the right stage of harvest of inflorescence was when 1/3 flowers were open on the spadix. Inflorescence harvested at this stage with medium to large size spathe lasted for a longer period (23.33 days). Among the pulsing treatments, BA 50 ppm for 12 hrs, recorded maximum vase life (20.00 days). Among the different holding solutions tried, 8-HQ 30 ppm, BA 20 ppm and Triadimefon 30 ppm recorded the optimum values of PL W, uptake of vase solution, change in pH and EC, and recorded late spathe blueing, late spadix necrosis and high gloss retention. Highest vase life period (27.00 days) was recorded in 8-HQ 30 ppm (25.00 days) and Triadimefon 30 ppm (25.00 days). Among the combinations tried, BA 20 ppm + Bavistin 0.1 per cent showed longest vase life (23.67 days). Maximum net profit per pot (Rs.204.60) was recorded by the treatment combination involving 80 per cent shade + Ohio solution + 750 ppm BA.ThesisItem Open Access Leaf litter dynamics in acacia and eucalyptus plantations(Department of soil science and agricultural chemistry, College of Agriculture, Vellayani, 1997) Moossa, P P; KAU; Venugopal, V KAn investigation entitled “Leaf litter dynamics in Acacia and Eucalyptus plantations” was undertaken to study the leaf litter production, litter decomposition and nutrient release characteristics, and impact of Eucalyptus and Acacia monoculture plantations on the soil physico-chemical and biological characteristics during 1994-97. One hectare each of Acacia auriculiformis, Eucalyptus tereticornis and a moist deciduous forest coming under the Kulathupuzha range of Kerala Forest Department were selected for the study. Field experiment were conducted to determine the leaf litter production and decomposition characteristics of plantation and natural forest. Leaf litter collected at monthly intervals showed that highest litter production was in Acacia (9.4 t ha-1 year-1) followed by natural forest (6.67 t ha-1 year-1) and Eucalyptus (4.68 t ha-1 yaer-1). Pattern of litter production was unimodal in Acacia and natural forest while in Eucalyptus it was bimodal. Chemical analysis of litter samples of plantations and natural forest revealed that with respect to nutrient content fresh and leaf litter of Acacia and Eucalyptus is superior to natural forest whereas litter quality in terms of water soluble components, polyphenols, lignin and cellulose were superior in natural forest. Annual dry matter loss of leaf litter by decomposition followed the order natural forest > Acacia > Eucalyptus. Nutrient release pattern of major and micro nutrients were also worked out for the leaf litter during the process of decomposition and natural forest litter recorded the highest mobility for all the nutrients on decomposition. Improvement in soil physico-chemical properties were noticed due to leaf litter decomposition in the monoculture plantation and natural forest. Biological activity in terms of earthworm and nematode under monoculture plantation was significantly lower than that of adjacent natural forest. Microbial content of soil below the litter bag during different months of decomposition were low in Eucalyptus and Acacia. Chemical analysis of the profile samples of various plantations revealed lower nutrient status in respect of major and micronutrients compared to natural forest. The physical properties of the soil also recorded as unfavourable change in the monoculture plantations as compared to the adjacent natural forest. Proximate anlysis of soil organic matter and humus characterization of the soil organic matter separated from various plantations indicated a low rate of humification and condensation under Eucalyptus. Low humic acid and fulvic acid content in humus and dominance of fulvic acid over humic acid was also noticed in Eucalyptus plantations. Functional group analysis of humic acid and fulvic acid showed that humic material separated from natural forest contained higher quantity of total acidity, contributed by COOH and phenolic group compared to plantation soils. UV, IR, DTA and TGA analysis of humic acid and fulvic acid separated from various plantations showed no variation with respect to natural forest. Allelopathic effect of fresh leaf and leaf litter extract of various plantations on the germination and growth of rice and cowpea followed the order Eucalyptus > Acacia > natural forest and as the concentration decreased from 1: 2 to 1: 10, the allelopathic inhibition also decreased significantly. Allelopathic effect of soil extract also followed the order Eucalyptus > Acacia > natural forest. Thus it can computed that though the leaf litter production and litter quality of monoculture plantations are comparable with that of natural forest, leaf litter decomposition and nutrient released were significantly lower than that of natural forest. Soil physico-chemical properties, soil fertility and biological activities were also adversely affected by monoculture plantations.ThesisItem Open Access Response of cucumber (Cucumis melo L.) to drip irrigation under varying levels of nitrogen and potash(Department of Agronomy, College of Agriculture, Vellayani, 1997) Lakshmi, S; KAU; Sasidhar, V KTwo field experiments and one observational trial were conducted in the Instructional Farm, College of Agriculture, Vellayani during 1992 and 1993 to study the effect of drip irrigation and application of N and K fertilizers on the growth and yield of cucumber. In the preliminary observation trial (Experiment I a) three levels of drip irrigation, (2, 3 and 4 I plant-1 day-1) four timings of irrigation (1, 2, 3 and 4 hours) and two number of drippers per plant (1 and 2 dripper plant-1) were evaluated and based on the results of this experiment, the number of drippers per plant and duration of drip irrigation was standardised. Experiment 1 b was conducted to standardise the method of application of fertilizers in Experiment 2 and 3 for which cucumber plants raised under different levels of drip irrigation (2, 3 and 4 I plant-1 day-1). This was conducted during April 1992. The results of these experiments indicated the duration for drip irrigation as 3 hours per day and one number of dripper per plant to be the best. The spread and depth of root system of cucumber plants raised under drip irrigation pointed out that the fertilizers as a ring around the base of the plant at a distance of 20 cm will be within the root zone of the plant. The experiments 2 and 3 were laid out in the field with three levels of drip irrigation (2, 3, and 4 I plant-1 day-1), three levels of nitrogen (35, 70 and 105 kg ha-1) with three levels of potassium (25, 50 and 75 kg ha-1) with three drip irrigation controls (2, 3 and 4 I plant-1 day-1) and farmer's practice. The yield of cucumber was highest at the drip irrigation level of 31 planr-1 day-1. Irrigation at this level favourably influenced the vegetative characters, yield attributes and yield. But root dry matter was not influenced by the different drip irrigation level while the root spread and root depth were highest at the drip irrigation level of 41 plant-1 day-1. When different levels of nitrogen applied were observed, the vegetative characters yield attributes and yield were found to be favourably influenced at 70 kg N ha-1. The root spread of cucumber was highest at the highest level of N applied (l05 kg ha-1). Potassium at 50 kg ha-1 produced the highest yield due to its favourable influence on the vegetative and yield characters. There was no influence of potassium on my of the root characters studied. The soil moisture status was highest up to 30 cm depth in all drip irrigated treatments while in pot watered plots, the soil moisture was higher in the lower layers below 30 cm. The field water use efficiency was highest at the drip irrigation level of 3 I plant-1 day-1, 70 kg nitrogen ha-1 and 50 kg potassium ha-1. The nitrogen, phosphorus and potassium uptake by the plants and fruits were highest at the drip irrigation level of 31 plant day I, 70 kg N ha-I and 50 kg K ha-I. The physical optium levels of drip irrigation was 31 plant -1 day -1, 93 kg N ha-1 and 65 kg K ha-1 in the first season. When another crop is repeatedly grown in the same field, the Nand K levels can be reduced to 75 kg ha-1 and 60. kg ha-1 but drip irrigation is required at the rate of 31 plant-1 day-1. This resulted in higher benefit cost ratio of 2.83 and internal rate of returns of 23%. The payback period of this project worked out to 1.13 years.ThesisItem Open Access Soil test crop response studies in cassava in laterite soils of Kerala(Department of Agronomy, College of Agriculture, Vellayani, 1997) Kumari Swadija, O; KAU; Sreedharan, CAn investigation was undertaken at the College of Agriculture, Vellayani to establish soil test crop response correlation for soil test based balanced fertilizer prescription for cassava var. M-4 in laterite soils of Kerala. The field investigation consisted of fertility gradient experiment, STCR experiment and technology verification trial. The fertility gradient experiment was conducted during April- May 1994 in the Instructional farm, Vellayani. The desired gradient in soil fertility was created in one and the same field by applying graded doses of N, P and K fertilizers and raising fodder maize var. African Tall. The STCR experiment was conducted in the same field during June '94-March '95 using the test crop, cassava var. M-4. The treatments consisted of factorial combinations of four levels of N (0, 50, 100 and 150 kg ha-1), three levels of P (0, 50 and 100 kg P2O5 ha-1) and five levels of K (0, 50, 100, 150 and 200 kg K20 ha-1) along with three levels of farmyard manure (0, 6.25 and 12.50 t ha-1) fitted in a response surface design. Using multiple regression model, the fertilizer adjustment equation for N at varying soil test values for available N for maximum tuber yield (t ha-1) of cassava in laterite soil was derived as FN = 136.6 - 0.2 SN where FN is fertilizer N (kg ha-1) and SN is soil available N (kg ha-1). The equation becomes FN = 136.6 - 0.2 SN - R for economic tuber production where R is the ratio of cost of one kg of fertilizer N to price of one kg of tuber. At varying soil test values for organic carbon% (OC) the above equations become FN = 226.13 - 378.13 OC for maximum tuber yield and FN = 226.13 - 378.13 OC - 1.25 R for economic tuber yield. The behaviour of fertilizer P and K was found to produce responses other than 'normal' and hence optimisation could not be done for fertilizer P and K for maximum and economic tuber yield at varying soil test values. The nutrient requirements of cassava var. M-4 were estimated to be 6.58, 2.37 and 6.28 kg N, P2O5 and K2O respectively to produce one ton of tuber. In the laterite soil, the efficiencies of contribution of nutrients from the soil for cassava were calculated as 40.17, 41. 3 3 and 48.60% N, P2O5 and K2O respectively. The fertilizer efficiencies were worked out as 54.38, 47 and 52.650% N, P2O5 and K2O respectively. The efficiencies of contribution of nutrients from farmyard manure were calculated as 78.24, 57.33 and 69.66%) N, P2O5 and K2O respectively. From the above basic data, fertilizer prescription equations for specific yield targets of cassava var. M-4 in the laterite soil were derived as given below. Without FYM FN = 12.10 T - 0.74 SN FP2O5 - 5.04 T - 2.02 SP FK2O = 11.93 T - 1.10 SK With FYM FN = 12.10 T - 0.74 SN - 1.44 ON F P2O5 - 5.04 T - 2.02 SP - 2.79 OP FK2O = 11.93 T - 1.10 SK - 1.58 OK where FN, F P2O5 and FK2O are fertilizer N, P2O5 and K2O respectively in kg ha-1, T is the target of tuber yield in t ha-1, SN, SP and SK are soil available N, P and K in kg ha-1 respectively and ON, OP and OK are quantities of N, P and K supplied through organic manure in kg ha-1 The technology verification trial was undertaken during June '96-March. '97 in the laterite soil in three farmers' fields in Thiruvananthapuram district and also in the Instructional Farm, Vellayani. The treatments consisted of Package of Practices recommendation for fertilizer, fertilizer recommendation by the Soil Testing Laboratory and fertilizer doses for the yield targets of 15, 20 and 25 t ha-1 along with a uniform dose of 6.25 t ha-1 of farmyard manure for all treatments. The fertilizer doses based on targeted yield equations recorded higher tuber yield and net income, benefit cost ratio and net returns per rupee invested over the fertilizer recommendation by the Soil Testing Laboratory and Package of Practices recommendation emphasising the need for site specific recommendation based on soil tests. The yield targets of 15 and 20 t ha-1 recorded more than cent per cent achievement and the yield target of 25 t ha-1 recorded about 90% achievement. Higher tuber yield, benefit cost ratio and net returns per rupee invested could be achieved for the yield target of 20 t ha-1. The fertilizer dose for the yield target of 15 t ha-1 recorded the highest returns per rupee invested on fertilizers. The study has revealed the superiority of fertilizer application based on targeted yield approach over the semi-quantitative approach employed in the soil testing laboratories and generalised state level Package of Practices recommendation for the crop. In this approach, the fertilizer dose can be adjusted in accordance with the specific objective and available resources of the farmer.