Theses

Browse

Search Results

Now showing 1 - 9 of 117

Open Access
Biology of neoplastic cells of ethmoid carcinoma
(Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1985) Madan Sing, Karki; KAU; Rajan, A
Attempts were made to propagate the ethmoid carcinoma cells in vitro to study the biological behaviour of the neoplastic cells and their relationship with the host. Efforts were also made to transplant the neoplastic cells in laboratory animals and tumour bearing natural hosts so as to lay out a model system for further investigation. In vitro propagation was tried by obtaining the tumour tissues from nine cows and three goats. Cells were grown in Hank’s balanced salt solution (HBSS). TC – 199 and Dulbecco’s modified Eagle’s medium with 10 to 20 per cent calf serum. The cell suspension was seeded into tissue culture bottles, milk diluting bottles and test tubes and incubated at 370 C. No growth was observed in the cultures made from the tumour tissue of cows. The tumour cells from two goats grew in monolyerand were maintained for nine days. There was no difference in growth of cells in different media employed. Insulin supplementation in the media did not cause any difference in the growth of the cells. Subsequently the cells died even after subculturing and changing of the media. Absence of certain unknown factors required by the neoplastic cells for growth was considered responsible for failure of the growth. Transplantation studies were carried out in autologous, homologous and heterologous species of animals using the tumour tissue framgments, homogenate, filtrate and cell suspension. No autograft or heterotransplant were successful. Homologous transplantation resulted in a firm painless growth at the site of injection. The growth initiated a cell – mediated immune response at the site of implantation. Failure of neoplastic cells to grow in the other hosts was considered to be due to rejection of the transplants by the host’s immune system.
Open Access
Regional anaesthesia of the hind-limbs in oats using lignocaine hydrochloride
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1985) Prahlad, Sapkota; KAU; Raveendran, S
A study on intravenous regional anaesthesia (IVRA) of the hind – limbs was conducted in 18 bucks, weighing 15 to 25 kg body – weight (in four groups). Lignocaine hydrochloride 2% colution was used as the anaesthetic. Four dose levels viz., 4 – 5 mg; 5 - 6 mg; 6 – 7 mg and 7 – 9 mg/kg body – weight were employed. A tourniquet was applied around the limb above the stifle joint. After a 10 min period of pre – injection tourniquet ischaemic, the anaesthetic was administered through saphenous vein following exsanguination. Anaesthetic effect was ascertained by pin – pricking and pinching the interdigital space. In nine animals the effect of IVRA on wound healing was studies. The onset and duration of anaesthesia and the time for waning away of anaesthesia were also recorded. Onset of anaesthesia was noticed in 2.50 + 0.84; 2.0 + 0.82; 2.86 + 1.34 and 2.43 + 1.40 min. Duration of anaesthesia in the four groups was 25.0 + 7.77; 24.86 + 3.80 ; 21.43 + 6.08 and 30.71 + 4.92 min respectively including a short duration after the release of tourniquet. The complete disappearance of anaesthetic effect was noticed by 6.17 + 4.54; 4.28 + 1.38 ; 5.43 + 2.07 and 3.86 + 1.21 min after the release of tourniquet. The anaesthetic effects were first apparent at the phalangeal region and progressed gradually upwards to the level of tourniquet and waned away in the reverse order. The healing of wound was uneventful and the histological study revealed that there was no variation in the healing process between the experimental and control groups.
Open Access
Studies on the pathogenicity of tetrameres mohtedai and acuaria spiralis of fowl
(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1977) Ramaswamy, K; KAU; Sundaram, R K
Controlled experiments were conducted at three levels of monospecific and dispecific infections with two commonly occurring spirurid worms of fowls viz. Tetrameres mohtedai and Acuaria spiralis. The blood studies conducted in monospecific T. mohtedai infection revealed a normocytic normochromic anaemia and eosinophilia during the initial phase and later a macrocytic anaemia, heterophilia and slight monocytosis by 66th day post infection. The infected birds lost considerable amount of their body weight. In layers there was a great delay in sexual maturity and poor feed efficiency as indicated by greatly reduced egg production. The percentage of establishment of worms in these experiments were found to be inversely proportional to the number of juveniles administered. The pathological changes in the affected proventriculi indicated an acute inflammation during the invasive phase of juveniles, followed by a chronic reaction which by 55th day post infection was well established indicating the development of a host – parasite balance towards the later stages of infection. Among the blood changes in monospecific A. spiralis infection, eosinophilia was evident initially followed by a marked leucopenia and development of a macrocytic hypochromic anaemia. Other effects due to parasitism were, delayed maturity, reduced rate of egg production, poor feed efficiency, loss of weight, emaciation and a mortality rate of 25% in chicks. The gross pathological changes in Acuariasis included the appearance of an irregular whitish patch on the serosa and formation of nodular growths in the mucosa which in heavy infections occupy the whole of the mucous membrane. Juveniles of A. spiralis were found to invade the compound glands in very heavy infections. The microscopic changes indicate a severe acute inflammation during the initial stages. Later as the disease became chronic pedunculated fibro – adenamatoid growths were evident on the mucous membrane. In dispecific infections with T. mohtedai and A. spiralis the haematological changes observed were eosinophilia during the early stages followed by a l eucopenia and heterophilia. As the disease progressed a macrocytic hypochromic anaemia developed. There was a great decrease in the live body weight of the infected birds. A mortality of 33.33% were observed in chicks with heavy infection. In layers the sexual maturity was delayed and egg production was greatly suppressed with consequent poor feed utilization. The gross and microscopic changes found in the affected proventriculi indicated a combination of lesions observed under monospecific infections of both the parasites. The results obtained show that the loss due to morbidity (mainly as a result of decreased body weight and reduced rate of egg production) was considerable. These facts indicate that both T. mohtedai and A. spiralis are economically important.
Open Access
Chloral hydrate for general annaesthesia in goats
(Department of Surgery, College of Veterinary and Animal Sciences,Mannuthy, 1981) Viswanathan, R; KAU; George, P O
Thirtysix apparently healthy alpins – malabari cross- bred bucks aged from 17 to 30 months and weighing from 22 to 39.5 kg were used for the present study. They were divided into two groups viz., Group I and II, consisting of 18 animals in each. Each of these groups was further divided into three subgroups viz. A, B and C, consisting of six animals in each. Freshly prepared chloral hydrate six per cent solution was administered intravenously at the rate of 1.0, 1.5 and 2.0 ml per kg bodyweight, while triflupromazine hydrochloride (Siquil) at the rate of 0.2 mg per kg bodyweight was also administered intravenously as the premedicant , in group II. Following were the salient results obtained during the present study: When the dose of chloral hydrate was increased, 1) Disappearance of palpebral reflex was quicker and duration was longer, 2) Setting in of incoordination of movements was quicker and the time for assuming the normal gait was longer and 3) Recumbency was seen only in higher doses. Administration of triflupromazine hydrochloride (siquil) as premedicant , increased the efficiency of anaesthetic effect in these animals. Temperature variation consequent on the administration of chloral hydrate, with and without premedication , was not significant the variation being less than 10 P. There was an increase in the rate of respiration and pulse within five minutes of administration of the drug (s), which showed a gradual downward trend. There was reduction in the erythrocyte count, haemoglobin and packed cell volume, while there was slight increase in the leucocyte count. On the basis of the present study, intravenous administration of chloral hydrate, six per cent solution, at the rate of two ml per kg bodyweight, may be recommended in goats, for surgical procedures of about half an hour durations. Premedication with triflupromazine hydrochloride at the rate of 0.2 mg per kg bodyweight would give better results.,
Open Access
Bacteriological quality of goat milk
(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1985) Chakhesang, Kevesiei; KAU; Sukumaran, M V
A total of 376 aseptically – drawn milk and 46 farm pooled milk samples were collected from the AICRP on Goats for Milk, Mannuthy and subjected to various tests to determine the bacteriological quality. An increase of over 59 and 188 – fold in bacterial number from aseptically – drawn milk to production – and milk for the morning and the evening milkings respectively was noticed. In aseptically – drawn milk samples 61.17% and 64.83% gave a SPC of below 100/ml in the morning and evening respectively. In farm pooled milk samples the SPC exceeded 105/ml in 39.13% of the morning and 65.22% of the evening samples. No psychrophilic bacteria was detected in any of the aseptically – drawn or farm pooled milk samples. The mean thermophilic counts of aseptically – drawn as well as farm pooled milk samples was less than one/ml. Though the mean coliform count of aseptically – drawn milk was found to be less than one/ml, the farm pooled milk samples showed 3,413 and 2734/ml for the morning and the evening samples respectively. The mean MBRT of the morning and the evening samples was 14.91 and 8.93 h for aseptically – drawn milk and 4.57 and 3.83 h for farm pooled milk samples respectively. The correlation coefficient between MBR time and SPC of farm pooled milk of the morning and evening samples was not significant (- 0.21 and - 0.27). The keeping quality of the morning and the evening milk samples (280 C) obtained was respectively 50.48 and 44.70 h for aseptically – drawn milk and 12.87 and 12.04 h for farm pooled milk samples respectively. The correlation coefficient between SPC and keeping quality of farm pooled milk samples was significant (- 0.46) for morning sample while that of evening milk was not significant (+ 0.28). The correlation coefficient between MBRT and keeping quality of farm pooled milk samples was also not significant in both the morning and the evening (+ 0.31 and + 0.13).
Open Access
Utero-tubal insufflation technique for diagnosis of tubal impatency in cows
(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1981) Athman, K V; KAU; Neelakanta Iyer, C P
The objective of the present study was to design an apparatus for utero-tubal insufflation test and to standardize its use in both excised genitalia and in live animals for the diagnosis and treatment of tubal impotency. Perfect obturation of the cervix was obstained using a stainless steel catheter with 22 FR. Neo Foley’s latex catheter sleeved over. Unilateral and bilateral impotency were recorded in 8.70 and 19.25 per cent of the excised genitalia. The incidence of unilateral and bilateral impotency in 37 infertile animals were found to be 2.70 and 13.52 per cent respectively. The mean pressure at which air escaped from both the tubes in excised genitalia was 128.12 mm. Hg. In cows and 148.93 mm. Hg. In heifers. On the contrary in experimental animals the corresponding pressure in cows and heifers were 116.38 mm. Hg. And 127.50 mm. Hg. Respectively. It was observed that a higher pressure was required to open both the tubes in heifers than in cows. Blown up condition of the uterus took place in excised genitalia of cows and heifers between 100-280 mm. Hg. And 100-260 mm. Hg. Respectively. The pressure range for producing “blown up” condition in experimental animal was found to be 120-220 mm. Hg. In cows and 195-200 mm. Hg. In heifers. Since the safety margin was estimated to be 60.00 mm. Hg. In experimental cows, the test is considered to be a safe diagnostic test for tubal impotency. Although uterus might be “blown up” in a very few cases at a pressure lower than the higher pressure for escape of air from normally patent tubes, the test will not endanger the life of the animal. Since 69.56 percentage of cows with bilateral patency of salpinx on testing conceived after insemination, utero-tubal insufflation test could also be exploited for the treatment of temporary tubal blockage.
Open Access
Studies on the postnatal development of the epididymis in the Malabari goat (capra hircus)
(Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1975) Harshan, K R; KAU; Radhakrishnan, K
The postnatal development of epididymis was described and illustrated from studies in 39 Malabari goats, divided into 13 groups, ranging from day old to 180 days of age. The weight of epididymis was positively correlated to age and body weight. The epididymal weight was more significantly correlated to body weight than to age. The left epididymis was found to be significantly heavier than the right. The length and thickness of the head, the body and the tail of the epididymis increased with increase in the weight of the organ. The tunica albuginea was found to be fibro- muscular in structure. The interstitial tissue was fibrous. The diameter of the epididymal duct and its lumen increased with increase in age. Both these measurements were highest in the cauda, less in the corpus and least in the caput. The process of pseudostratification of the epithelial lining of lumen of the duct was completed at first in the cauda, secondly in the corpus and finally in the caput. Regional differences in the height of epithelium was noticed, the highest being in the caput, lower in the corpus and lowest in the cauda. The epithelial height increased with advance in age. Sperms were observed at 165 days of age in the lumen of the epididymal duct. The concentration of sperm was maximum in the cauda, slightly less in the corpus and least in the caput. The lumen of the epididymis of the 150 days and 165 days old goats showed some spherical cells with spherical nuclei. These were considered to be spermatogenic cells coming from the testis.
Open Access
Study on the influence of cattle keeping on the bacteriological quality of domestic well water
(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 1981) George Oommen, T; KAU; Soman, M
Wells are the main sources of water supply in Indian villages and towns as elsewhere in any of the rural areas of the developing tropical countries. The shallow, open, dug wells are liable to contamination from the surrounding sources by various means. Faecal contamination of water is considered as one of the major causes of health hazards resulting in innumerable diseases among people throughout the world. Therefore, the bacteriological analysis of water is aimed at the detection and enumeration of indicators of faecal pollution. The movement of animal excreta into surface and ground water is considered a major factor contributing to the bacteriological pollution of available water in many regions. In order to assess the influence of cattle keeping on the bacteriological quality of domestic well water in Mannuthy area, the present study was undertaken. Forty wells from households randomly selected, with and without cattle keeping, for the study spreading over for a period of six months covering the summer and south-west monsoon during 1981. Water samples were collected aseptically, once in each season. They were bacteriologically analysed for SPC, MPN of coliforms and the detection and enumeration of E.coli. FS and C1 perfringens. Bacterial counts in water from all wells far exceeded the standards recommended for open, shallow wells. Although an apparently higher SPC in water from households with cattle keeping than from those without was observed, it was not statistically significant. The seasonal variation in the counts showed a hundred-fold increase in the case of SPC during monsoon than summer. But the similar increase in the MPN was negligible. The indicator bacteria, especially E.Coli and C1.perfringens showed a significant reduction during monsoon. The sanitary survey of the wells conducted with the help of a ready made performa revealed that none of the wells could be considered satisfactory either in construction or maintenance. The SPC and FS counts were found to have a positive correlation with the depth of wells. The SPC was positively correlated with the type of latrine also. The MPN of coliform and the depth of lining and distance of cattle shed from well were negatively correlated; so also a negative correlation was observed in the case of FS count and the distance between latrine and well. The various characteristics of wells, cattle keeping and latrines were found to have a significant correlation with the SPC and MPN of coliforms. When these of wells and latrines were alone considered, they showed significant correlation only with FS count. But it was observed that the characteristics of wells and latrines alone could explain for the considerable variations in the SPC and MPN than those of cattle keeping, as a source of pollution. It is concluded that the type of construction of well do not have any direct bearing on the bacteriological quality of well water except in the case of Cl. perfringens counts. Cattle keeping has also no significant influence on the bacteriological quality of domestic well water in Mannuthy area though all wells under study showed evidence of recent or past faecal contamination.
Open Access
Experimental studies on rumeno-rectal fistula in calves
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1980) Sumangala, M; KAU; Muraleedharan Nair, K N
Chronic tympany of the rumen is common in cattle. Rumen fistulation has been reported to be successful in the treatment of chronic tympany. Left side external fistulation of rumen has been adopted by many workers. But this was accompanied by seepage of rumen contents and soiling of the flank. While screening the available literature, no reports on an internal rumen fistulation could be found. The effects of rumeno – rectal fistula in experimental calves were studied in the present work. The study was conducted on 12 male calves in two groups , each group consisting of six calves. In the animals of Group – I , laparotomy was performed on the right flank and the right dorsal sac of rumen was anastomosed to the anterior part of the rectum. In the animals of Group II, laparotomy was performed on the left flank and the left dorsal sac of rumen was anastomosed to the anterior part of the rectum. The PH of the rumen contents and the rectal washings, presence of rumen protozoa in the rectal contents, haemoglobin content, packed cell volume, total and differential leucocyte count, nature of dung and the body weight were noted before fistulation and at regular intervals after fistulation. The animals were kept under observation for a period upto 100 days. Closure of the fistula was performed 60 days after fistulation, in two calves, one from each group. The details as described above were noted in these animals also after closure of the fistula. The PH of rectal contents before surgery ranged from 7.3 to 7.5. From the second day after surgery, it ranged between 7.0 and 7.2 in both the groups. The erythrocyte count and haemoglobin content showed a progressive reduction in eight animals and packed cell volume showed a reduction in 10 animals. The variation in the total and differential leukocyte counts were within normal limits. Rumen protozoans were present in the rectal contents from the second day after surgery. Loss of body weight was noticed in all the six animals of Group –I, whereas an increase in body weight was noticed in four animals of Group – II. The feeding habits of the calves were normal. Dung was loose in consistency from the second day after surgery and it contained more fluid soon after drinking water. One animal of Group – I died on the 46th post – operative day, and another one was sacrificed on the 53rd post-operative day. Surgical closure of the fistula was performed in two animals, one from each group. All other animals were sacrificed between 60th and 100th post-operative day. At autopsy omental adhesions were noticed at the site of anastomosis in six calves. The fistula was patent and had a diameter of about 2 cm in 11 animals. Rumen contents were present in the rectum. The site of anastomosis was hard to touch and no other abnormalities could be detected in the rumen and rectum. Complete encapsulation of sutures at the site of anastomosis was noticed in four calves. The healing at the site of anastomosis was complete in all the animals. In the two calves in which closure of the fistula was performed, the healing was uneventful and there was progressive improvement in haemogram values and body weight. It is concluded that functional rumeno – rectal fistula could be established in calves and that it did not cause any untoward clinical manifestations. There was escape of rumen contents through the fistula to the rectum. Surgical closure of the fistula could also be done successfully.

Theses

Browse

Thesis Type

Type

Author

Date

Subject

Search Results