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ThesisItem Open Access Comparison of polymerase chain recation with conventional methods for the diagnosis of leptospirosis(Department of Microbiology, College of Veterinary and Animal Sciences,Mannuthy, 2003) Elaiyaraja, M; KAU; Jayaprakasan, VA study was undertaken to standardize the peR technique for the diagnosis of leptospirosis and to compare the efficacy of peR with DFM and culture in the diagnosis of leptospirosis. Two sets of primers, namely, primers A and B derived from Leptospira interrogans serovar canicola rrs (16S) gene and G I and G2 derived from genomic library of L. interrogans serovar icterohaemorrhagiae, were evaluated to amplify 12 reference strains of leptospires representing the serovars viz., australis, rachmati.; canicola, grippotyphosa, hardjo, hebdomadis, icterohaemorrhagiae, pomona, poi, pyrogenes, tarassovi and patoc. The primers A and B specifically amplified all the serovars tested, while the primers Gland G2 failed to amplify the serovar patoc. The primers A and B which amplified a 331 base pair fragment of leptospiral DNA were used for the routine detection of leptospires in clinical samples. Restriction enzyme digestion of the primer A and B amplified product with the enzymes Dde I and MnI I and direct sequencing established the identity of the amplified product. A total of 192 samples were collected from different sources like human, dogs and bovines with suspected history of leptospirosis and from rodents. All were tested by peR and the positivity ranged from 33.3 to 54.2 per cent. Of the samples collected 125 samples were tested by all the three techniques viz., peR, DFM and culture and the results were compared. The peR technique was found to be more sensitive, specific and rapid, over conventional methods as it detected 41.6 per cent, compared to 25.6 per cent by DFM and 2.4 per cent by culture, of the samples tested.ThesisItem Open Access Synergistic effect of alfatoxin B1 and ochratoxin A in chick embryos(Centre for Excellence in Pathology, College of Veterinary and Animal Sciences,Mannuthy, 2001) Rony Ray John, KAU; Lalithakunjmma, C RThe present study was taken up to investigate the synergistic effects of the mycotoxins AFBl and OA on chick embryos, the variations in the susceptibility to the toxins both individually and in combination. Embryomortality, teratogenicity and reduced embryonic weight were some of the deleterious effects observed in the inoculated chick embryos. The abnormalities seen were more in the combined toxin treated group and they included stunted embryos,' evisceration, herniation of yolk sac, beak abnormalities, cranioschisis and oedema of head and neck. Both the liver and kidney were equally damaged and extensi ve haemorrhage into the parenchyma of all the organs was the main histological lesion observed in addition to degenerative and necrotic changes. This study showed that mycotoxins deleteriously affect a number of parameters associated with embryonic growth.ThesisItem Open Access Studies on Lice Infesting Domestic Animals And Birds(Department of Parasitology, College of Veterinary and Animal Sciences,Mannuthy, 1979) Mohan, M C; KAU; Sathianesan, VIn a study on the lice of animals and birds in and around Trichur and their control, cattle was found to be infested with Haematopinus, quadripertuous and linognathu vituli. Buffaloes with haematopinus tuberoulatus, goats with linognathus africanus and damalinia cappre dogs with heterodoxus longitatsus. Elephatnts with haematomyzus elephantis and poultry with Menacanthus stramineus menopon gallinae, Goniodes gigas and Lipeurus caponis. Their incidence and intensity were maximum in winter and minimum in summer. Their morphology has been described in detail. For their control, Nuven, Malathion and sevin were tried and Nuven (0.05 percent) was found to be superior to the other two insecticides, with 100 percent efficacy.ThesisItem Open Access Knowledge of foot and mouth disease among dairy farmers in thrissur district and the constraints in adopting control measures(Department of Veterinary and Animal Husbandry Extension Education, College of Veterinary and Animal Sciences, Mannuthy, 2009) Subin K, Mohan; KAU; Rajkamal, P JDairy farmers generally had an ambivalent or neutral attitude towards FMD vaccination. Between FMD experienced and inexperienced there wasn’t a significant difference in the attitude. Attending seminars, group discussions and lecture classes on FMD was positively and significantly correlated with attitude. Awareness of symptoms and transmission, first aid and disease management and prevention and control measures were generally found to be either medium or low. However, FMD inexperienced had comparatively lower awareness. Media exposure, exposure to inter personal channels of communication, seminars and workshops attended were found significant in explaining variations in the general awareness of FMD. Regarding constraints faced by farmers in adopting control measures, the foremost one was farm families hiding or not reporting that their cattle was FMD affected. In the case of disease management, the foremost constraint reported was the much time consuming nursing process of affected animals. The most relevant constraints reported by the implementing officers of FMD vaccination programme were unwillingness of dairy farmers to vaccinate their animals due to reduction in milk yield, the laborious nature of door step vaccination and the possible chances of vaccination after effects like abscess formation, abortion and lameness.ThesisItem Open Access Adoption of indigenous and modern animal husbandry practices among the tribes of Attappaddy in Palakkad district(College of Veterinary and Animal Sciences, Mannuthy, 2010) Bimal P, Basheer; KAU; Rajkamal, P JDegree of belief and extent of adoption of selected indigenous and modern animal husbandry practices and the determinants of adoption were studied among the tribes of Attappady. Age and years of experience in animal husbandry were positively and significantly correlated with degree of belief in indigenous animal husbandry practices whereas, negatively and significantly correlated with degree of belief in modern animal husbandry practices. The psychological, communication and extent support variables studied were negatively and significantly correlated with degree of belief and extent of adoption of indigenous animal husbandry practices whereas, positively and significantly correlated with extent of adoption of modern animal husbandry practices. Significant differences between the deep and peripheral areas were observed in the case of both degree of belief and extent of adoption. A total of fifty three indigenous animal husbandry practices, under the domains viz:- selection, feeding, breeding, housing, management practices, diseases and treatment and other customary practices were studied. Their strength and rationality were also determined. Similarly a total of fifty one modern animal husbandry practices, under the domains viz:- selection, feeding, breeding, housing, management practices and disease and treatment were studied. Strength of these practices were also determined. Further, level of adoption of rational and irrational indigenous animal husbandry practices besides that of modern animal husbandry practices were determined. Highly adopted irrational indigenous animal husbandry practices and lowly adopted modern animal husbandry practices were identified. Similarly the extent of adoption of rational and irrational indigenous animal husbandry practices besides that of modern animal husbandry practices were studied. It was noticeable that, in the case of both rational and irrational indigenous animal husbandry practices the extent of adoption was medium to high. In the case of modern animal husbandry practices the extent of adoption was medium to low.