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ThesisItem Open Access Evaluation of bacteriological quality of beef carcasses in meat processing plant(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 2003) Sethulekshmi, C; KAU; Nanu, EDuring the present study, 40 beef carcasses were randomly selected from a meat processing plant located at Kochi in Kerala. The plant procures beef carcasses from two slaughtering units, viz., Source A and B, located in Tamil Nadu. From each carcass surface 500-cm2 area was swabbed which consisted of 100 cm2 each from neck, brisket, loin, flank and outer round. The samples from each carcass were examined for the bacterial quality by estimating the total viable count (TVC), coliforms count (CC), Escherichia coli count (ECC) and faecal streptococcal count (FSC). All samples were also subjected to the isolation and identification of Escherichia coli, Staphylococcus aureus, Salmonella and Listeria monocytogenes. The samples of air, water, equipment and hand wash of personnel were also collected and estimated the various bacterial loads of these samples. Analysis of variance test of the data did not reveal significant difference between the mean total viable count of the samples from two sources. The samples had an overall mean total viable count of 7.40 ± 0.17 10glO cfu/cm/. The mean count of samples from source B was slightly higher than that from source A. The count of the samples belonging to source A ranged from 106 to 109 cfu/cm2 while the count of the samples from source B varied between 106 and 108 cfu/cm/. Of the 40 carcass samples examined, 45 per cent had count at the level of 106 cfu/crrr', The counts in 30 per cent and 22.5 per cent were at the level of 108 cfulcm2 and 107 cfu/cnr', resp~ctively. Analysis of variance' test of the data revealed a significant (P<0.05) difference between the mean coliforms count of the samples from the two sources. The samples from source A had a higher mean count. The overall mean coliforms count of the samples was 3.41 ± 0.13 10glO cfu/cm". The count of the samples from both the sources varied from 102 to 104 cfu/cm/. The count in 21 (52.5 per cent) carcass samples was at the level of 103 cfu/crn", In 17.5 per cent carcasses the count was at the level of 104 cfu/crrr' and in 30 per cent carcass samples the count was at the level of 102 cfu/crrr'. Analysis of variance test of the Escherichia coli count revealed a significant (P<0.05) difference between the mean count of samples from the two sources. The samples belonging to source A had a higher mean count. The overall mean Escherichia coli count of the samples was 1.83 ± 0.22 10glO cfu/crrr'. The Escherichia coli count of the samples from both the sources varied from 101 to 103 cfu/cm2. The count in 16 (40 per cent) of carcass samples was at the level of 102 cfu/cm", In 17.5 per cent samples each had count at the levels of 101 and 103 cfu/cm", Analysis of variance test of the faecal streptococcal count revealed significant (P<0.05) difference between the mean counts of samples from the two sources. Source A had higher mean count. The overall mean count of samples was 3.27 ± 0.10 10glO cfu/cm". The count on the carcasses from the source A ranged between 102 to 104 cfu/crrr' whereas the count on the carcasses belonging to source B varied between 102 to 103 cfu/crrr'. Out of 40 carcasses, 18 (45 per cent) had count at the level of 103 cfu/crrr'. The counts on 40 and 15 per cent of the carcasses were at the levels of 102 to 104 cfu/cm/, respectively. A significant (P<0.05) positive correlation was observed between the mean CC and FSC of the carcasses belonging to source A. The association between the mean TVC and FSC in source B was significant (P<0.05). Escherichia coli was isolated from 10 carcasses belonging to source A and 5 carcasses from source B. Out of the 15 isolates, 14 were serotyped and were grouped under nine serotypes and one isolate was untypeable. The serotype 0157 was isolated from two of the carcass samples belonging to source A. Four isolates from this source belonged to serotype 036. The serotypes 036, 0156, 0157 and 0172 were isolated only from the samples obtained from the source A. The serotype 08 was isolated from both the sources. However, the serotypes 013,065,069 and 075 were isolated only from samples of source B. Staphylococcus aureus was isolated from two (5 per cent) carcasses belonging to source A and one (2.5 per cent) of the carcasses belonging to the source B. Of-the 40 carcass samples tested, salmonellae were isolated from one sample each from the source A and B. Listeria monocytogenes could not be isolated from any of the samples belonging to both the sources. The mean total viable counts on meat-cutting board and meat-cutting table were 4.94 ± 0.87 and 4.28 ± 0.87 10glO cfu/cm/, respectively. The mean coliforms count on the former was 1.24 ± 0.94 10glO cfu/crrr' and the latter was at the level of 1.26 ± 0.83 10glO cfu/crrr'. The mean faecal streptococcal count was at the level of 1.38 and 1.29 10glO cfulcm2 on meat cutting board and meat cutting table, respectively. Ice samples had a mean total viable count of 3.20 ± 0.11 10glO cfu/ml. The coli forms count in ice, pond, and tap water were 2.30 ± 0.08, 1.39 ± 0.77 and 0.50 ± 0.21 10glO cfu/ml, respectively. The mean total viable count, coliforms count, Escherichia coli count and faecal streptococcal count observed per ml of the hand wash of the personnel engaged in various operations were 4.96 ± 0.82, 1.26 ± 0.78, 0.80 ± 0.24 and 1.48 ± 0.77 IOglO cfu/ml, respectively. The mean bacterial load in the air samples of slaughter hall and chilling room obtained in the present study was 100.7 ± 8.17 and 8.75 ± 1.19 cfu/min, respectively.ThesisItem Open Access Evaluation of bacteriological quality of processed chicken(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 2003) Raji Rose, Jacob; Nanu, EAn investigation was carried out to evaluate the bacterial quality of chicken carcasses collected at two steps on the dressing line of a meat processing plant. During the study a total of 60 dressed chicken carcasses consisting of 3 0 each were randomly collected after the removal of head and feet (ARHF) and after evisceration (AB) from a meat processing plant located at Cochin. The bacterial quality was evaluated by estimating the total viable count (TVC), coliforms count (CC), Escherichia coli count (ECC) and faecal streptococcal count (FSC). All the samples were examined for the presence of Escherichia coli, salmonellae, Staphylococcus aureus and Listeria monocytogenes. Bacterial quality of environmental samples and processing equipmerits were also evaluated. The study revealed a significant (P<0.05) difference between the mean total viable count of the samples taken ARHF and AB. A higher mean count (5.88 ± 0.13 10glO cfulml) was observed in the former group of carcasses. The count of the carcasses taken after the removal of head and feet ranged between 104 and 107 cfulml while that of eviscerated carcasses vary from 104 to 106 cfulml. A higher mean coliforms count (3.92 ± 0.12 10glO cfulml) was observed in the samples taken from the carcasses after evisceration. The count of the carcasses in both groups varied between 102 and 1 O~ cfulml. Analysis of variance test of ' the mean Escherichia coli count revealed significant (P<0.05) difference between the count of the samples taken from the carcasses ARHF and AB. The eviscerated carcasses had higher count (2.15 ± 0.24 10glO cfulml) .. The mean Escherichia coli count of the carcasses taken ARHF was 0.89 ± 0.23 10glO cfulml. In both groups, the counts ranged from 102 to 103 cfulml. Escherichia coli was not detected in 63.33 per cent of carcasses taken after the removal of head and feet and 23.33 per cent of eviscerated carcasses. All samples collected from the carcasses after the removal of head and feet and after evisceration had faecal streptococci but the mean count of the samples belong to both groups did not differ significantly. !\ higher mean count (3. I) I :l.: 0.07 10glO cfu/ml) was obtained in the samples collected from the eviscerated carcasses. The samples taken after the removal of head and feet had a mean count of3.89 ± 0.0610glO cfulml. Correlation coefficient of the data revealed a significant (P<0.05) and positive association between the mean TVC and FSC, CC and ECC, CC and FSC and ECC and FSC of the samples taken from the carcasses after the removal of head and feet. A similar relationship was observed between the mean CC and ECC \ and ECC and FSC of the eviscerated carcasses. Escherichia coli was isolated from 36.67 per cent of carcasses taken after the removal of head and feet and 76.67 per cent of eviscerated carcasses. A total of 40 Escherichia coli isolated from both the groups were serotyped and grouped under 15 serotypes, two rough strains and one untypable. The serotypes consisted of 02, 05, 08, 016, 021, 051, 060, 064, 069, 078, 081, 0101, 0119, 0123 and 0140. Salmonella enteritidis was isolated from three (10 per cent) carcasses taken after the removal of head and feet. Eleven (36.67 per cent) samples taken from the carcasses after evisceration revealed the presence of Staphylococcus aureus. Listeria monocytogenes could not be isolated from both the groups of carcasses. The mean total viable count of air samples from the processing hall was high (1.99 ± 0.83 10g1O cfu/min) followed by sticking and bleeding area (1.63 ± 0.98 10glO cfu/min) and scalding area (1.54 ± 0.01 10g1O cfu/min). The water samples from the scalding tank had TVC, CC, ECC and FSC but pond and tap water samples, defeathered carcass wash and fmal carcass wash did not reveal the presence of Escherichia coli and faecal streptococci. Among the equipments, samples from the eviscerating table had TVC, CC and FSC but the samples from the defeathering machine and knife swab had only TVC and Cc.ThesisItem Open Access Evaluation of bacteriological quality of pork carcasses in a processing plant(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 2003) Shiny John; KAU; Nanu, EDuring the present study, 25 pork carcasses were randomly selected from the dressing line of a pork processing plant, in order to evaluate the hygienic status of the carcasses produced in the plant. From each carcass, after evisceration and also after final washing, 500 Clll2 area was swabbed, which consisted of 100 cm2 each from jowl, shoulder, bacon, loin and ham areas. Samples collected, after evisceration were pooled into 500 1111 diluent, which formed the initial test samples. Similarly, the initial test sample was also prepared with the samples collected from the finally washed carcasses. This sample was tested to evaluate the bacterial quality of carcasses by estimating total viable count, coliforms count, Escherichia coli count and faecal streptocoecal count. The sample was also used for the isolation and identification Escherichia coli, Staphylococcus aureus, Salmonella and Listeria monocytogenes. Samples from environment, processing equipment, hand wash of personnel, scalding tank water and HOPE pouches were also tested to estimate the bacterial load. Analysis of variance test revealed signi ficunt (Peviscerated and finally washed carcasses. The mean count in the former and latter groups or carcasses was 5.14 L O.I() and 5.31 :L 0.16 loglo cfu/cm", respectively. In ()4,20 and le) per cent or the eviscerated carcasses the count was at levels 105, 104 and 10.1 cfu/cm", respectively. III tile latter group or carcasses, eight, 56,32 and four per cent had the count at the level of 10(1, 105, 104 and io' cfu/crn", respectively. The mean coli forms count of eviscerated carcasses was 1.89 ± 0.17 10g\O cfu/cm ' and the count in the finally washed carcasses was 1.99 ± 0.23 10glO cfu/crn". night and 24 per cent ni' samples bclongiru; to both groups had tile count at the level of 102 cfu/cm". The count ill 52 ami 24 per cent or eviscerated and finally washed carcass samples was at the level or lolcru/C\\l2. Eviscerated carcasses had a mean Escherichia coli count of 1.19 ± 0.08 loglo cfu/cm ' and the count ill finnl ly washed carcasses was 1.29 :I: 0.12 loglo cfu/cm", The organism was not detected in RO and R4 per cent or eviscerated and finally washed carcasses, respectively. Tile count in I () and four per cent of the former group or carcasses was at levels I {)2 and I ()I Cl'U/CI1l2. respectively. In eight per cent each of finally washed carcasses, the count was at the level of 102 and 101 cfu/cm2. '~he eviscerated and finally wash cd carcasses had a mean faecal streptococcal count of 3.19 ± 0.20 and 3.28 ±O.20 loglo cfu/cm, respectively. The count on 12 per cent of carcasses in each of the above groups, did not reveal the organism. However, in both the groups 01' carcasses, 20 pCI' cent or samples each had the count at 103 cfu/crrr'. The count in 52 pCI' cent of the eviscerated and 60 pCI' cent of the finally washed carcasses was ut the level or 102 cf'U/CIl12. The count at the level of 101 cfu/cm2 was observed in I () pCI' cent or the eviscerated and eight pCI' cent of the finally washed carcasses. Analysis 01' the data revealed positive and significant (P<0.05) association between CC and ECC in eviscerated and finally washed carcasses. However, a negative but signi ficant (P 0.05) correlation was observed between TVC and CC and TVC and ECC in the finally washed carcasses. Escherichia coli was isolated from 20 per cent of eviscerated carcasses and 16 per cent of finally washed carcasses. Out of 18 isolates recovered from both the groups, 15 fell into 12 scrotypcs and three were untypablc. The serotypes include 05,08,011, 025,060,064,078,088, OlOl, 0119, 0123 and 0140. Staphylococcus aureus was isolated from eight and 24 per cent of samples belonging to eviscerated and finally washed carcasses, respectively. None of the carcass samples in both the groups revealed the presence of Salmonella and Listeria monocytogenes. Air samples at the evisceration area had the highest bacterial count and the lowest was observed at the chilling room. Pond water samples had the highest mean total viable count and coli forms population was seen highest in scalding tank water. Escherichia coli count was highest in hand washing of personnel. None of the pond and tank water samples revealed presence of the organism. Samples collected from the bell semper had the highest mean total viable count and least in packaging material. Samples obtained from carcass splitter had the highest coliforms count. None of the processing cquipmcnts revealed the presence of Escherichia coli. Cutting board samples showed the highest faecal streptococcal, count.ThesisItem Open Access Bacteria of public health significance in broiler dressed chicken(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 2002) Bindu Raj, R; KAU; Nanu, EIn the present investigation, 60 chicken carcasses, consisting of 20 each, collected from retail shops selling dressed birds which were brought from outside the state (A), shops selling locally reared dressed birds (B) and dressed chicken from the Kerala Agricultural University Poultry Farm (C). The bacterial quality of each carcass was evaluated by estimating the total viable count (TVC), coliforms count (CC), Escherichia coli count (ECC) and faecal streptococcal count (FSC). All samples were also tested for the presence of bacterial pathogens such as Escherichia coli, salmonellae, Staphylococcus aureus and Listeria monocytogenes. Analysis of variance test of the mean TVC revealed significant (P<0.05) difference between the count of the samples from source Band C. The overall mean TVC of the samples was 7.89 ± 0.07 10glO cfulml of the carcass rinse. The samples from source B had the highest mean TVC (8.06 ± 0.11 log., cfulml) and lowest mean count was seen in the samples belonging to the source C (7.69 ± 0.13 log., cfulml). The samples from source A had a mean TVC of7.94 ± 0.08 log., cfulml. The count at the level of 109 cfulml was observed in one of the samples belonging to the source B. The count in three (5%), 34 (56.67%) and 22 (36.67%) samples was at the level of 106, 107 and 108 cfulml, respectively. Analysis of variance test showed significant (P<0.05) difference between the mean CC of the samples from source A and C and Band C. The overall mean CC of the carcass rinse was 4.97 ± 0.1 0 log., cfulml. The samples from the source A had the highest mean count (5.30 ± 0.10 log}o cfulml) and lowest in the samples from source C (4.37 ± 0.17 log., cfulml). The mean CC of samples from source B was 5.23 ± 0.13 log}o cfulml. The count in l.67 per cent and five per cent of the samples was at the level of 102 and 106 cfulml, respectively. The count in 1l.67, 3l.67 and 50 per cent samples was at the level of 103, 104 and 105 cfulml, respectively. Analysis of variance test revealed significant (P<0.05) difference between mean ECC of the samples belonging to the source A and C and Band C. The overall mean ECC of samples from the three sources was 2.20 ± 0.27 log}o cfulml. The mean count of the samples belonging to the source A, Band C was 2.83 ± 0.48, 2.66 ± 0.46, 1.08 ± 0.35 10glO cfulml, respectively. The count in five, 20 and 26.67 per cent of the carcasses was at the level of 102, 103 and 104 cfulml, respectively. Analysis of variance test revealed highly significant (Pdifference between FSC of samples from source A and B, A and C and B and C. The overall mean FSC of the samples was 4.32 ± 0.09 log., cfulml. The highest mean count was observed in samples from source A (4.99 ± 0.10 log., cfulml) and the lowest mean count in samples from source C (3.75 ± 0.l0 10glO cfulml). The mean FSC in samples from source B was 4.20 ± 0.10 10glO cfulml. The count in 38.33, 46.67 and 15 per cent samples were at the level of 103, 104 and 105 cfu/ml, respectively. Correlation coefficient test of the data revealed significant (P<0.05) association between the mean CC and FSC. A positive and non- significant correlation existed between mean TVC and CC, TVC and ECC, TVC and FSC, CC and ECC and ECC and FSC. Escherichia coli was isolated from 51.67% of the samples. The 44 E. coli isolated from the samples were serotyped and fell into 21 serotypes, six rough strains and two .untypables. The serotypes consisted of 05,08, 014, 025,033,041,049,066,078,081,084,085,091,0116, 0121, 0131, 0132, 0146, 0150, 0157 and 0161. Salmonellae were isolated from two samples. Staphylococci were isolated from 30% of the samples, but none of them were coagulase positive. All samples were found free from Listeria monocytogenes.ThesisItem Open Access Bacterial profile of market beef and It`s public health significance(Department of Veterinary Public Health,College of Veterinary and Animal Sciences, Mannuthy, 2000) Deepa, Jolly; KAU; Nanu, ETbe bacterial quality of75 market beef samples, collected from four different areas, in and around Thrissur was evaluated. The samples were collected from East Fort (EF), Mannuthy (MN), Shakthanthampuran market (SM) and West Fort (WF), areas. Of the samples, 25 belonged to EF, 21 to MN, 17 to SM arid 12 to WF, areas. All samples were subjected to estimate the total viable-count, coliform, Escherichia coli. faecal streptococcal and Staphylococcus aureus counts and efforts were also made to isolate and identify Escherichia coli, Salmonellae and Staphylococcus aureus. Analysis of variance test of the data en total viable count revealed no significant difference between the mean count of the samples from the four areas. The overall mean total viable count was 7.39 ± 0.06 log to cfu/g. Samples from the MN area had the highest mean count (7.57 ± 0.11 log to cfu/g) , while samples from WF area had the lowest mean count (7.19 ± 0.15 log-Io cfu/g). The mean count of the samples from EF and SM areas were 7.29 ± 0.10 Iogto cfu/g and 7.45 ± 0.12 log to cfu/g, respectively. Of the total samples examined, 60.00,25.33 and 14.67 per cent of samples had the count at the level of 107, 106 and 108 cfu/g, respectively. A highly significant (p<0.01) difference was observed between the mean coliform count of samples from the four areas. Critical difference test of the count revealed significant difference between the mean count of samples from EF and MN, EF and SM, and WF and SM, areas. The overall mean coliform count of the samples was 4.62 ±0.06 logto cfu/g. The mean coliform count of the samples from EF, WF, SM and MN, areas was 4.35 ± 0.10, 4.50 ± 0.15~ 4.95 ± 0.12 and 4.76 ± 0.11 logto cfu/g, respectively. The level of coliform count in 54.67 per cent of samples was 10" cfu/g. The count in 29.33 per cent samples was 105 cfu/g and in 16 per cent it was 103 cfu/g. No significant difference was observed between the mean Escherichia coli count of samples from the four areas. The overall mean E. coli count of the samples , was 3.52 ± 0.09 10glO cfu/g. The highest mean count (3.67 ± 0.19 10glO cfu/g) was observed in samples from the SM area, while the lowest (3.28 ± 0.23 10glO cfu/g) was observed in samples from the WF area. The mean counts of samples from EF and MN areas were 3.41 ± 0.16 10glO cfu/g and 3.65 ± 0.17 10glO cfu/g, respectively. Of the , samples examined, 53.33 per cent had a count at the level of 103 cfu/g. In 26.67 per cent samples, the count was at the level of 104 cfu/g and in 16 per cent samples, the count was at the level of 102 cfu/g. Highly significant (p<0.01) difference was observed between the mean faecal streptococcal count of samples from the four areas. Critical difference test of the data revealed significant difference between the mean count of samples of EF and MN, WF and MN, and WF and SM, areas. The overall mean count of the samples was 4.11 , ± 0.07 10glO cfu/g. The highest mean count (4.38 ± 0.12 10glO cfu/g) was observed in the samples from MN area, whereas the lowest (3.75 ± 0.16 10glO cfu/g) was s-een in the samples belonging to WF area. The mean count of samples from EF and.Slvl areas was 3.93 ± 0.11 10glO cfu/g and 4.28 ± 0.13 10glO cfuig, respectively. The count in 49.33 per cent of the samples was at the level of 103 cfu/g. The count in 42.67 per ceru and 8 per cent samples was at the level of 104 &.i1d 105 cfuig, respectively. A significant (pviable count and faecal streptococcal count, coliform and Escherichia coli counts and coliform and faecal streptococcal counts. A positive but non-significant correlation was observed between total viable count and coliform count, and between E. coli and faecal streptococcal counts. The correlation between total viable count and E. coli count was negative and non-significant. Of the 75 samples, 98.67 per cent showed the presence of Escherichia coli. From these samples, a total of 185 isolates were identified as E. coli. Of these, 91.35 per cent were identified as E. coli biotype I and 8.65 per cent as biotype 11. Salmonella and Staphylococcus aureus could not be isolated from any of the samples. Cent per cent of the samples examined in the study, conformed to the standards prescribed for Salmonella, by the International Corrunission on , Microbiological Specifications for Foods, while only 25.33 per cent met the total viable count limit.ThesisItem Open Access Isolation and identification of certain bacteria of public health importance from market beef(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 2001) Binsy, Mathew; KAU; Nanu, EIn the present investigation, 100 retail beef samples were collected from retail shops located at East Fort (EF), West Fort (WF) Sakthanthampuran Market (SM) and Mannuthy (MN) areas. From each area 25 samples were collected and were brought to the laboratory in a thermocool container. All samples were subjected to estimate Total Viable Count (TVC), Coliforms count (CC), Faecal streptococcal count (FSC) and Escherichia coli count (ECC) and also for the isolation and identification of Escherichia coli, salmonellae Staphylococcus aureus, and Listeria monocytogenes. The overall mean total viable count of the samples belonging to four areas was 6.66 ± 0.08 10glOcfu/g. The mean total viable count of EF, WF, MN and SM areas was 6.61 ± 0.15, 6.68 ± 0.17, 6.49 ± 0.15 and 6.84 ± 0.1910glOcfu/g respectively. One, 13 and 23 per cent had counts at the level of 109, 108 and 107 cfu/g respectively and 46 per cent and 17 per cent of the samples had counts at the level of 106 and 105 cfu/g. The overall mean coliforms count was 2.73 ± 0.09 log.scfu/ g. The mean coliforms counts obtained from the samples of EF, WF, MN and SM areas were 2.78 ± 0.16, 2.84 ± 0.14, 2.62 ± 0.22 and 2.70 ± 0.18 10glOcfu/g respectively. The percentage of sample that had counts at the level of 104, 103 and 102 were two, 46 and 45 respectively. The overall mean Escherichia coli count was 3.56 ± 0.17 10glOcfu/g. The mean Escherichia coli counts of EF, WF, MN and SM areas were 3.11 ± 0.38,4.00 ± 0.27; 3.52 ± 0.37 and 3.60 ± 0.34 10glOcfu/g respectively. One, 19, 45 and 17 per cent of samples had counts at the level of 104, 103, 102 and 10 cfu/g respectively. The overall faecal streptococcal count was 3.04 ± 0.09 10gIOcfu/g. The mean faecal streptococcal counts of EF, WF, MN and SM areas were 3.06 ± 0.16, 3.16 ± 0.08, 2.77 ± 0.28 and 3.18 ± 0.17 10gIOcfu/g respectively. Eleven, 53 and 30 per cent samples had counts at the level of 104, 103 and 102 cfu/g respectively. There was no significant difference in all the four counts between the four areas. The overall correlation between the 100 samples collected from the four areas showed a highly significant (P~O.O I) and positive correlation between TVC and FSC, CC and ECC, CC and FSC and ECC and FSC. A significant (P~0.05) and positive correlation was observed between TVC and CC. Only 18 per cent samples met the standards prescribed by Government of India and 63 and 61 per cent samples met the standards prescribed by ICMSF and Oregon state respectively for total viable count. Twenty and 34 per cent samples met the standards prescribed by Government of India .and Oregon state for Escherichia coli count respecti vely. None of the samples yielded Listeria monocytogenes and salmonellae, but 82 per cent samples had Escherichia coli. The serotypes obtained were 03, 019, 022, 025, 029, 034, 036, 042, 050, 051, 053, 055, 065, 066, 073,079,0105,0109,0115,0139,0140,0147,0152,0163, 0164 and 0173. Two per cent of the samples had coagulase positive staphylococci.