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Subject: Veterinary Pharmacology and Toxicology × Author: KAU × End date: 2006 × Start date: 2006 × Type: Thesis ×
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    ThesisItemOpen Access
    Antipyretic and CNS activity of seeds from red and white types of lotus (nelumbo nucifera) in albino rats
    (Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, 2006) Deepa, P K; KAU; Usha, P T A
    The antipyretic and CNS activity of the extract of the seeds of red and white Nelumbo nucifera was studied in albino rats. Qualitiative tests for the detection of phytochemicals showed the presence of steroids, alkaloids, flavonoids, glycosides, phenolic compounds, diterpenes, triterpenes and saponins. Pyrexia was induced by subcutaneous injection of 20% Brewer’s yeast suspension. Group I served as pyrexia control, group II was administered aspirin at the dose rate of 100 mg/kg body weight, Group III and IV with extract of red lotus seed and group V and VI with extract of white lotus seed at the dose rate of 400 and 600 mg/kg body weight respectively. The body temperature was recorded from zeroth to fifth hour at one hour interval. Haematological parameters and Serum ALT, AST levels were estimated at peak of pyrexia and five hours after drug administration. For CNS study group I was kept as normal control, Group II was administered chlorpromazine at the dose rate of 7 mg/kg body weight. Group III , IV V and VI were treated as in the case of antipyretic study. Actaphotometer and Rotarod were used to assess the spontaneous and forced motor activities respectively. Haematological parameters and serum ALT, AST levels were estimated at peak of tranquillization. The extract of white lotus seed at dose rate of 600 mg/kg body weight showed maximum antipyretic effect followed by red lotus at 600 mg/kg body weight and white lotus at the rate of 400 mg/kg body weight among the treatment groups. By the fifth hour the effect of white lotus seed extract at the dose rate of 600 mg/kg body weight was comparable to that of standard drug aspirin. Eventhough the extract of red lotus at the dose rate of 600 mg/kg body weight inhibited the spontaneous motor activity to the maximum initially, the extracts were found to be equipotent after 120 minutes of drug administration. But the activity was less when compared with the standard drug chlorpromazine. Comparison of the treatment groups showed that group IV showed maximum inhibition of forced motor activity followed by group VI and group III. The haematological and biochemical parameters assessed were within the normal range in both studies. From the present study it can be concluded that the extracts of red and while lotus seeds possessed potent antipyretic and CNS effects. The extract from the seeds of white lotus showed more potent CNS activity where as both the extracts were showing equipotent tranquillizing property.
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    ThesisItemOpen Access
    Anti-inflamatory and analgestic actions of red and white lotus seeds (nelumbo nucifera) in albino rats
    (Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, 2006) Vikrama Chakravarthi, P; KAU; Gopakumar, N
    Anti-inflammatory and analgesic effects of Nelumbo nucifera (lotus) seeds, red and white types were assessed and compared in albino rats. The carrageenin induced paw oedema was one of the acute inflammatory models followed in anti-inflammatory screening. The acute and chronic type pain models viz., tail flick method and experimental neuropathy model of analgesic activity, respectively, were adopted for analgesic screening. Diclofenac potassium at the rate of 3 mg/kg was used as a standard drug for both the studies. In cyclooxygenase-2 enzyme inhibition assays the celecoxib @ 10mg/kg was used as a standard drug. Both red and white types of lotus seeds at the dose rate of 400 mg/kg and 600 mg/kg were taken for the anti-inflammatory and analgesic studies. Anti-inflammatory effect of red and white lotus seeds was found to be effective in all phases of carrageenin induced inflammation. The higher dose groups of lotus seed extracts were revealed more inhibition than their corresponding lower dose. While comparing all groups, the higher dose group of white lotus seed, exhibited more pronounced inhibition of paw oedema than others. Analgesic effect was found to be significant in both acute and chronic analgesic models. The analgesic activity was more in tail flick method when compared to experimental neuropathy model. The cyclooxygenase-2 (COX-2) enzyme inhibition assays in both ELISA and spectrophotometer showed significant effect than control. The percentage of inhibition of COX-2 was more evidenced in both lotus seed extract of higher dose groups than its lower dose. However, the higher dose groups of white lotus seed exhibited more control over inhibition of COX-2 enzyme than others. . In case of biochemical parameters in both anti-inflammatory and analgesic screening the serum cholesterol level was found to be decreased in treatment groups when compared to control. Even though, there was a reduction in serum cholesterol level, all the values were within the normal range. The rise in serum level of AST and ALT in both inflammation and analgesic models were noticed in lower dose treated group. Conversely, there was a reduction in level of enzyme was noticed in higher dose treated groups. However, the values were with in the normal level. Both the studies showed the haematological parameters viz., total leukocyte and differential counts were within the normal range in all groups, even though a non-significant increase in neutrophil count than lymphocyte was noticed in carrageenin induced inflammation and tail flick method in rats.