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ThesisItem Open Access Physiological and molecular studies on genera of loranthaceae and their management(Department of Plant Physiology, College of Agriculture, Vellanikkara, 2021) Garggi, C; KAU; Girija, THemiparasitic plants belonging to the family Loranthaceae are major tree parasites. Dendrophthoe falcata, Helicanthus elastica, Macrosolen capitellatus are major hemiparasites infecting perennial crops of the tropics. Helixanthera wallichiana and Taxillus tomentosus are two important hemiparasites infecting the fruit and timber trees of the high ranges. Apart from being a troublesome parasite they also serve as a linchpin reservoir supporting an extensive ecosystem. The prevailing investigation namely “Physiological and molecular studies on genera of Loranthaceae and their management” was carried out at Department of Plant Physiology, College of Agriculture, Vellanikkara, Thrissur, during the period from 2015 to 2020. Morphological characters of representative species viz., Dendrophthoe falcata, Helicanthus elastica, Macrosolen capitellatus, Helixanthera wallichiana and Taxillus tomentosus from five selected. Samples of these were collected from various locations from the plains and highranges of Thrissur. In all the selected species, leaves were oppositely arranged, D. falcata and H. elastica had oblong shaped leaves, leaves of M. capitellatus was lanceolate in shape, and H. wallichiana and T. tomentosus had narrow ovate shaped leaves. Fruit was berry in all the genera. Hemiparasites possess a physiological structure called haustoria through which they abstract water and minerals from the host. Haustorial branching pattern in all the genera was found to be basal epicortical root (ber). Haustorial portions of the collected samples were treated and prepared to permanent slides. Anatomical sections of the haustoria of the five selected genera of Loranthaceae revealed that there exists a transition zone between the host- parasite interphase region. This was observed as undifferentiated parenchymatous cells which aid in translocation of molecules. Presence of xylem elements were observed at the haustorial region. Haustorial anatomy of M. capitellatus was unique as there was complete merging of cells of host and parasite at the interphase region. The lineage of five selected genera belonging to Loranthaceae family were studied by molecular assay. The results from the ISSR assay revealed that, T. tomentosus and M. capitellatus had maximum similarity compared to all the other genera, since it formed a separate cluster. Morphological characterization indicated similarity between the two genera in the haustorial attachment pattern. Both the species have a single point of attachment to the host. H. wallichiana was observed to be more similar to the first cluster. D. falcata formed another branch close to H. wallichiana, where all these four genera formed the main group. H. elastica formed a separate lone group, which indicated genetical variance from other genera studied. Morphological observations supporting such a uniqueness in this parasite was noted in haustorial branching type, where basal epicortical root with lateral tendril like structures from nodes and internodes were observed. The parasite also showed other phylogenetically advanced characters according to Angiosperm Phylogeny Group (APG system) of classification like fused corolla and calyx. To study the host parasite interaction, root feeding studies with labelled 32P were undertaken. Cocoa plants infected with both parasites D. falcata and H. elastica was selected for the study. 32P in carrier solution of 1000ppm orthophosphoric acid @ 2 mCi per plant was fed to the surface running roots of cocoa. Leaf samples of the host and parasite were collected at 7 and 14 days after treatment and assayed for radio activity. To understand the translocation from parasite to host, leaves of the parasitic species both D. falcate and H. elastica were also smeared with labelled 32P and leaf samples were analysed from different parts of both the host and the parasite. These experiments were done on different trees. Leaf samples of the host and parasite were collected at 2, 7 and 14 DAT and assayed for radio activity. Results from the radio assay indicated that there is bidirectional movement of nutrients from host to parasite and parasite to host. There exists a prioritized partitioning pattern for nutrient transport (phosphorous) based on demand by the sink, regardless of the parent plant. The host and parasite were found to act as a single system indicating their coexistence. Physiological parameters of the host and parasites were also observed using InfraRed Gas Analyser (IRGA) for a period of six months. It was observed that stomatal conductivity of D. falcata and H. elastica was significantly higher than the host cocoa. Even though there was no much variation in the photosynthetically active radiation (PAR) received by the host and parasite during the period of observation, D. falcata and H. elastica had significantly higher rate of photosynthesis than cocoa. Transpiration rate of both parasites were significantly higher than their host. Carboxylation efficiency and light use efficiency of H. elastica were observed to be significantly higher than the host, cocoa. Field experiment was conducted to improve the management strategy and control the spread of the parasite. Use of surfactant was found to improve the efficacy of ethephon in controlling the regrowth of parasite on host plant. Non- ionic surfactants viz., Nonyl phenol ethoxylate (NPE) and Organosilicone (OS) were selected for the study. A combination of OS (0.5 ml/L) surfactant with ethephon (25ml/L) was successful in suppressing the regrowth of both D. falcata and H. elastica.ThesisItem Unknown Evaluation of CO2 enrichment on growth, development and soft rot tolerance in ginger (Zingiber officinale Rosc.)(Department of Plant Physiology, College of Agriculture, Vellayani, 2021) Manasa, R; KAU; Manju, R VThe present investigation entitled “Evaluation of CO 2 enrichment on growth, development and soft rot tolerance in ginger (Zingiber officinale Rosc.)” was undertaken with the objective to assess the impact of elevated CO 2 on growth, yield, quality and tolerance to soft rot in ginger through morphological, physiological, biochemical and molecular analysis. Two sets of pot culture experiments were conducted during 2017- 2019. The technology utilized for CO 2 enrichment was Open Top Chamber (OTC) system established under Department of Plant Physiology, College of Agriculture, Vellayani. In the first experiment three varieties of ginger Aswathy, Athira and Maran were utilized to assess the impact of elevated CO 2 on growth, yield and quality of ginger. One set of three month old plants were shifted to OTC and another set was retained under ambient CO 2. The plants were harvested at 4 th , 5 th , 7 th and 8 th months after planting (MAP). Observations on growth, physiological and biochemical parameters were recorded at each harvest. Yield, yield parameters, tissue nutrient status and quality parameters were assessed at 8 MAP. Second experiment was conducted to assess the impact of elevated CO 2 on tolerance to soft rot in three ginger varieties Aswathy, Athira, Maran and wild ginger congener Zingiber zerumbet. One set of three and a half month old plants were shifted to OTC and another set retained at ambient CO 2 and were challenged with Pythium aphanidermatum at 4 MAP. The CO 2 concentration of 500 ppm was maintained in the OTC. The results of the first experiment indicated an improvement in growth parameters like plant height, number of leaves, root weight, shoot weight, stem diameter and number of tillers, physiological and biochemical parameters like total chlorophyll, photosynthetic rate and total sugars in the rhizome by recording significantly higher values upon CO 2 enrichment when compared to ambient CO 2 condition. Significant reduction in transpiration rate, stomatal conductance and specific leaf area were recorded upon CO 2 enrichment. The per cent increase in yield parameters like number of fingers (38.19%), rhizome spread (11.32%), thickness (42.72%) and dry weight of rhizomes (31.43%) recordedhigher values upon CO 2 enrichment, based on average performance of all the selected varieties, leading to greater yield. The per cent increase in the fresh weight of rhizome recorded higher for variety Aswathy (38.34%), followed by Maran (20.00%) and Athira (12.12%) with a total fresh rhizome yield of 165.35 g plant -1 under ambient and 228.75 g plant -1 under elevated CO 2 condition in the case of Aswathy. Upon CO 2 enrichment the quality of rhizome was modified with increase in fiber (60.26%), gingerol (9.06%), phenol (19.25%), total oleoresin (11.52%), starch (44.84%) and essential oils (31.10%). Significant reduction in protein and nutritional status in the rhizomes were also recorded. Nitrogen, potassium, iron and zinc contents decreased by 15.78%, 3.22%, 11.78% and 18.57% respectively. Phosphorous and copper showed decrease in trend though not significant. Among the three varieties of ginger, Aswathy was found to be performing better compared to Athira and Maran not only in terms of yield, also for majority of quality parameters assessed upon exposure to elevated CO 2 condition. In the case of impact of elevated CO 2 on soft rot development, there was no variation in the per cent disease incidence. However, lesser number of days was taken for development of symptom. The per cent disease index was reduced by 11.10% upon CO 2 enrichment. The reduction in the fresh weight of rhizome upon pathogen inoculation was also less under elevated CO 2 (14.34%) when compared to ambient CO 2 (17.60%) showing that increasing CO 2 levels might bring down the disease severity. Upon P. aphanidermatum inoculation, the retention of photosynthetic pigments like chlorophyll a (0.90 mg g -1 FW) chlorophyll b (0.74 mg g -1 FW) total chlorophyll (1.64 mg g -1 FW) carotenoids (0.58 mg g -1 FW), xanthophyll (0.40 mg g -1 FW) and photosynthetic rate (5.92 μmol CO 2 m -2 s -1 ) were higher under elevated CO 2 when compared to ambient CO 2 . Higher membrane integrity values and lower malondialdehyde (0.75 μg g -1 FW) content in all the selected varieties were evident upon pathogen inoculation under CO 2 enrichment. Enhanced activity of ascorbate peroxidase (4.86 activity Units mg -1 total protein), PAL (Phenylalanine ammonia lyase) (20.91 Units mg -1 total protein), and glucanase (4.57Units mg -1 total protein) activity, as well as higher accumulation of phenols (32.40 mg g -1 DW) and ascorbic acid (7.14 mg 100 g -1 FW) were found in the inoculated plants under CO 2 enrichment compared to the ambient CO 2 . The catalase activity was found to reduce upon pathogen inoculation and the reduction was higher (0.11 Units mg -1 total protein) under ambient CO 2 as compared to elevated CO 2 (0.09 Units mg -1 total protein). Highest levels of hydrogen peroxide, superoxide and cell death were observed upon pathogen inoculation under ambient CO 2 through staining techniques and it was highest for variety Aswathy and least for Z. zerumbet. After pathogen inoculation maximum values for variable to maximum fluorescence Fv/Fm (0.63), photosynthetic electron transport rate (82.60 micro equiv m -2 s -1 ) and quantum yield of PS II photochemistry (0.29) were recorded under elevated CO 2. Protein profiling performed in varieties Athira and Aswathy revealed lower expressions of 20-25 kDa and 25-35 kDa protein under pathogen inoculation in variety Aswathy. The expression of 55 kDa (Rubisco Large Sub- Unit) was down regulated to a greater extent under pathogen inoculation in both the varieties. Higher expression levels of the defense responsive genes Phenylalanine ammonium-lyase (PAL), Cinnamyl alcohol dehydrogenase (CAD) and Chalcone synthase (CSH) were also observed upon pathogen inoculation under CO 2 enrichment in both the varieties. Between the varieties, Athira had higher relative fold changes of PAL, CAD and CSH under both ambient and elevated CO 2 upon pathogen inoculation. The present study revealed that CO 2 enrichment can improve the performance of ginger in terms of growth, yield and quality. It also indicates changes in photosynthetic allocation pattern and phytochemical profiles with enhanced contents of oleoresins and essential oils. Ginger plants showed better tolerance against P. aphanidermatum upon CO 2 enrichment. This was apparent in the form of reduced disease severity, oxidative stress level and less reduction in yield upon pathogen infestation under elevated CO 2 condition. This was evidently achieved through the activation of multiple mechanisms, including enhanced production of anti-oxidants and up regulation of pathogen resistance proteins. This is one of the first studies to comprehend on possible effects of elevated CO 2 on the susceptibility of ginger to soft rot. The study also demonstrated the varietal variations existing in the response to CO 2 enrichment which will help in selection ofsuitable varieties for changing climatic scenario. The information generated indicates the possibility of enhancing both qualitative and quantitative yield of ginger through low cost CO 2 enrichment technologies. The insights obtained on the tolerance mechanisms involved will help to restructure the soft rot disease management strategies in the coming future.