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Subject: Plant Breeding × End date: 2018 × Start date: 2018 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Evaluation of CMS based rice hybrids developed from rice varieties of Kerala identified as restorers
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2018) Nayana Jyothibas, KAU; Jayalekshmy, V G
    The present study entitled “Evaluation of CMS based rice hybrids developed from rice varieties of Kerala identified as restorers” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2016-2018, with an objective to evaluate CMS based rice hybrids developed from rice varieties of Kerala as restorers, for heterosis for yield and grain quality. The study was conducted in two experiments i.e., 1) Hybrid seed production 2) Evaluation of hybrids. The first experiment, on hybrid seed production was undertaken in College of Agriculture, Vellayani during May to August, 2017 (Virippu, 2017). Two CMS lines viz., CRMS31A and CRMS32A were crossed with nine restorers. Restorers were sown in three staggers, 10 days before the sowing date of CMS lines, at the sowing date of CMS lines and 10 days after the sowing date of CMS lines. Synchronized flowering dates of the CMS and restorer lines were recorded. CRMS31A was crossed with Remya, Jayathy, Swarnaprabha, Kanakom and Neeraja. CRMS32A was crossed with Annapoorna, Aiswarya, Mattatriveni and Kanakom. Six crosses i.e., CRMS31A x Jayathi (H1), CRMS31A x Kanakom (H2), CRMS31A x Remya (H3), CRMS32A x Annapoorna (H4), CRMS32A x Kanakom (H5), CRMS32A x Mattatriveni (H6) that had given more than 1500 viable seeds were carried forward for hybrid evaluation. Date of sowing of the restorers and maintainers for getting synchronization in flowering to undertake hybridization was standardized in this experiment. In the second experiment, hybrids along with the better parent and checks were evaluated in a Randomized Block Design (RBD) with three replications during October 2017 to February 2018 (Mundakan) in IFSRS (Integrated Farming System Research Station), Karamana. Observations on 12 yield contributing traits and 8 grain quality traits were recorded in the hybrids. Genetic parameters calculated from these observations showed that the GCV and PCV values for the characters studied did not vary greatly indicating low influence of the environment in these characters. The mean performance of hybrids showed that the yield plot -1 was the highest for H5 (CRMS32A x Kanakom) followed by H3 (CRMS31A x Remya), H1 (CRMS31A x Jayathi), H2 (CRMS31A x Kanakom) and H4 (CRMS32A x Annapoorna). The hybrid H5 (CRMS32A x Kanakom) showed the highest mean values for plant height, number of filled grains panicle-1, length breadth ratio of grain and grain yield (g) plant-1. Pollen fertility of the hybrid H3 (CRMS31A x Remya) was superior to the standard check. This shows that the variety Remya is a complete restorer for male sterile cytoplasm CRMS31A. Hybrid H5 also showed pollen fertility on par with the check showing that the variety Kanakom is restorer for CRMS32A. The other hybrids H4 (CRMS32A x Annapoorna) and H6 (CRMS32A x Mattatriveni) had pollen fertility per cent around 50. So the male parents Annapoorna and Mattatriveni for CRMS32A can be considered as only partial restorers. The low level of pollen fertility was the reason for low yield of hybrids H4 (CRMS32A x Annapoorna) and H6 (CRMS32A x Mattatriveni) despite high number of productive tillers. Five out of six hybrids were red kernelled. It was found that the hybrids inherited the kernel colour from the male parents. The kernel length by breadth ratio of all the hybrids was significantly higher than that of Uma. The hybrids had a medium to slender grain shape (kernel length by breadth ratio 2.1 to 3 and above). This may be the reason behind the phenomenon of curling noted in cooked rice in all the rice hybrids studied. Standard heterosis for yield plot-1of the hybrids calculated over the standard check variety Uma was the highest for H5 (CRMS32A x Kanakom) followed by H3 (CRMS31A x Remya) and H1 (CRMS31A x Jayathi). Heterobeltiosis for yield calculated over the better parent was maximum for H1 (CRMS31A x Jayathi) followed by H5 (CRMS32A x Kanakom). Amylose content of most of the hybrids fell into intermediate category along with that of the check variety Uma, showing the hard and non sticky nature of the cooked rice of the hybrids. Cooking time for hybrids was less compared to that of Uma (29 minutes). This evaluation of hybrids could identify two promising hybrids for Kerala H5 (CRMS32A x Kanakom) and H3 (CRMS31A x Remya) which could give a yield advantage of 17.91% and 16.37% over the popular rice variety Uma. These two hybrids had long slender grain shape with red kernel colour. The amylose content of these two hybrids was also intermediate and low. These hybrids had a lower cooking time and good head rice recovery. Hence the hybrids can be recommended for Kerala after trials over locations and seasons. A better seed production technique has to be standardized before commercial release.
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    ThesisItemOpen Access
    Identification of water stress tolerant amaranthus genotypes (Amaranthus tricolor L.) with high yield and quality
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2018) Shahiba, A M; KAU; Beena Thomas
    The present study entitled “Identification of water stress tolerant amaranthus genotypes (Amaranthus tricolor L.) with high yield and quality” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2016-2018, with an objective to identify high yielding genotypes of amaranthus with good quality and tolerance to water stress. The study was conducted under two experiments. In the first experiment thirty accessions of Amaranthus tricolor L. available in the Department of Plant Breeding and Genetics and collected from other sources were evaluated for yield under field condition and morphologically described using IBPGR descriptor for the amaranthus. Madhur local (A22) recorded highest yield plant-1(125.926g) followed by Kalliyoor local (A4), Ayyanthole local (A28), Haripad local (A7), Palakkadu local (A2), Anachal local (A6), Aryanadu local (A21), Poonkulam local (A20), Kazhakkuttom local (A9) and Kannara local (A29). In the second experiment, these ten genotypes selected based on the yield were evaluated in a Randomized Block Design (RBD) with three replications during November 2017- December 2017. Water stress was imposed after 5 days of irrigation to water holding capacity to the transplanted seedlings by scheduling the irrigation at a depth of 20mm at 20mm CPE (Cumulative Pan Evaporation). Analysis of variance was calculated for all the characters under study and was found to be significant for all the genotypes evaluated. The mean performance of the genotypes for the characters were studied. The maximum yield was observed for the genotype A22 (Madhur local) followed by the genotype A9 (Kazhakkuttom local), genotype A20 (Poonkulam local) and genotype A2 (Palakkadu local) and the minimum yield was recorded for genotype A4 (Kalliyoor local). The genotype A22 (Madhur local) showed the highest mean values for stem girth, number of branches, length of leaf lamina, leaf to stem ratio, membrane integrity, relative water content, proline content of leaves, vitamin A and lowest oxalate content. The character Vitamin A content registered the highest GCV (41.22%) and PCV (41.25%). High heritability coupled with high genetic advance was observed for leaf width, number of branches, yield plant-1, protein content, fibre content and vitamin A. The yield plant-1was found to be significantly and positively correlated with leaf width, number of branches, yield plot-1, membrane integrity, proline content of leaves and vitamin A both at genotypic and phenotypic levels. Petiole length and percentage leachate were found to be negatively correlated with yield plant-1. Path analysis revealed that number of branches, yield plot-1and proline content of leaves had the maximum positive direct effect on yield plant-1. The results of the present study showed that genotype A22 (Madhur local) was superior in yield performance under water stress condition followed by the genotype A9 (Kazhakkuttom local), genotype A20 (Poonkulam local) and the genotype A2 (Palakkadu local). The genotype A22 (Madhur local) also recorded the maximum stem girth, number of branches, length of leaf lamina, leaf to stem ratio, membrane integrity, relative water content and proline content of leaves with high Vitamin A and low oxalate content. Presence of proline in the leaves might be considered as an important water stress tolerance mechanism.
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    ThesisItemOpen Access
    Identification of saline tolerant rice hybrids for pokkali tract
    (Department of Plant Breeding and Genetics, Vellanikkara, 2018) Nikhil Narayanan; Biju, S
    Various abiotic stresses causing a decline in global rice production are drought, salinity and chillness. Among these, salinity is the major factor that affects crop productivity. The traditional rice varieties grown under Pokkali area show remarkable tolerance to salinity and floods making it unique. However, the yield potential of these varieties is low. In India, out of ninety three rice hybrids developed, a few viz. DRRH 28, KRH 4, PSD 3, etc. were reported to have tolerance to salinity. Considering the potentials of hybrids, identification of salt tolerant hybrids suitable for Pokkali tract of Kerala is an urgent need of the hour. Hence, the study was designed to explore the adaptability of eight rice hybrids to the unique tract of Pokkali and evaluation of traits associated with salinity tolerance. The study comprised of two experiments, the first experiment was Laboratory screening for salinity tolerance and the second experiment was Field evaluation of rice hybrids in Pokkali tract. The laboratory screening was done following a completely randomised design and the genotypes were grown in Yoshida's solution and evaluated for various seedling characters. High shoot length, root length, high root CEC and high root and shoot proline content were seen in Vytilla-6 followed by Ezhome-2. Low root and shoot proline content were observed in IR-29 confirming its susceptibility to salinity. Among the hybrids, a high value for shoot length, root length, total number of roots, root CEC, root proline, shoot proline and survival per cent was observed in the hybrids CORH-3 and KRH-4. Vigour index was high in Sahyadri 4 followed by KRH-4 and low in MRP- 5401. Vytilla-6 and Ezhome-2 showed the high survival per cent while low survival per cent was noticed in Sahyadri 2 and MRP-5401. Vytilla-6 exhibited high chlorophyll content and RWC while low RWC was recorded in MRP-5401, Sahyadri- 2 and Sahyadri-4. Ca in root and shoot along with Na content in shoot were high in IR-29 while Vytilla-6 had low Ca and Na content. High Na in root was seen in Sahyadri 4 followed by Sahyadri 3. High amount of root Cl2 was recorded in Sahyadri-3, Sahyadri-1, Sahyadri-4, IR-29 and Sahyadri-2 while, the lowest amount of root and shoot Cl2 was in Vytilla-6. High amount of shoot Cl2 was seen in Ezhome-2 and IR-29. High amount of root and shoot SO4 content was seen in IR-29, Sahyadri-3, Sahyadri-4, Sahyadri-2 and Sahyadri-1. The lowest root and shoot SO4 content was recorded in Ezhome-2, followed by Vytilla-6. Low visual score of 3 was recorded in Vytilla-6 and Ezhome-2; a score of 5 was recorded for CORH-3, MRP-5401, TNAU RH-4 and KRH-4. Sahyadri-2, Sahyadri-3 and Sahyadri-4 were observed to have scored more than seven. Score 9 was noticed in Sahyadri-1 and IR-29, indicating that these genotypes are more susceptible to salinity. Visual score under salinity was found to be negatively correlated with shoot and root length, number of roots, vigour, proline content and RWC, while it was positively correlated with content of Ca, Na, Cl2, SO4 and K in the plant tissue. Field evaluation of rice hybrids in Pokkali tract was done during Kharif season of 2016-17, following a randomised block design. Days to fifty per cent flowering and maturity showed that all hybrids became early under salinity. Tall plants were observed in Vyttila-6 and Ezhome-2 and short plants in IR-29. High number of productive tillers was observed in CORH-3 and low in Sahyadri-2. Ezhome-2, IR-29 and Vyttila-6 had high number of spikelets per panicle while seed setting percentage and long panicles were observed in Ezhome-2. Spikelet sterility was high in Sahyadri-2 and low Ezhome-2. High 1000 grain weight was observed in Ezhome-2 and low in IR-29. Based on visual scoring Vyttila-6 was classified as highly tolerant and IR-29 as highly susceptible. Correlation studies indicated that grain yield per plant was positively correlated with productive tillers per plant and seed setting per cent, while it was found to be negatively correlated with sterility per cent. Visual scoring was negatively correlated with plant height, uppermost internodal length, days to maturity and seed setting per cent. The hybrid CORH-3 was found to be moderately tolerant based on visual scoring and it produced highest yield per plant indicating that this hybrid is better than saline tolerant traditional varieties.
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    ThesisItemOpen Access
    Genetic evaluation of F2 generation for yield and water stress tolerence in upland rice
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2018) Ivy Mary, Rajan; KAU; Lekha Rani, C
    The present study entitled “Genetic evaluation of F2 generation for yield and water stress tolerance in upland rice” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2016-2018. The objective was to evaluate the pattern of variability in F2 for yield and yield contributing traits under upland and drought situations and to select superior segregants combining drought tolerance and high yield. The study material included four parents and three F2 populations selected from the Ph.D project entitled “Genetic analysis of drought tolerance in rice (Oryza sativa L.)”. The study comprised of two experiments .In experiment- I, the parents and F2 segregants were raised under rainfed upland condition exposed to natural stress. Among the F2 populations, Vaishak x Harsha (T6) recorded the highest mean grain yield plantˉ1 (38.45g – 48.15g) and lowest mean spikelet sterility (7.76%-21.18%). T6 recorded the highest mean values for number of productive tillers plantˉ1 (9 - 16) and leaf area index (mean: 1.67). Vaishak x Vyttila 6 (T5) recorded the highest mean values for number of spikelets panicleˉ1 (149 - 178), harvest index (44.88% – 48.15%) and proline content (mean: 0.29mg g−1). Thottacheera x Harsha (T7) recorded the highest mean values for chlorophyll(mean: 1.78mg g−1) and leaf soluble protein content (mean: 9.36mg g−1). The data obtained was subjected to transgressive segregation analysis and in T5 more than 50 percent positive segregants were observed for number of productive tillers plantˉ1, spikelet sterility, number of spikelets panicleˉ1 and panicle length. T6 recorded high percentage of positive segregants for number of productive tillersˉ1, grain yield plantˉ1, plant height and spikelet sterility. In T7 percentage of positive segregants were the highest for spikelet sterility and number of productive tillers plantˉ1. Dendrogram drawn on the basis of squared Euclidean distances classified the genotypes into four clusters. Cluster I -T1,T5,T6, cluster II- T4, cluster III -T2,T7 and cluster IV-T3. In experiment II, another set of parents and F2 segregants were grown under rainshelter imposing reproductive stage moisture stress. Irrigation was given at 20mm depth once in seven days from panicle initiation stage onwards. Among the F2 segregants, T5 recorded the highest mean values for grain yield plantˉ1 (11.80g – 18.88g), number of spikelets panicleˉ1 (128 - 174), grain weight panicleˉ1 (2.60g – 4.33g ), harvest index (42.44 % - 68.13%) and proline content (mean: 0.57) and the lowest for spikelet sterility (5.50% - 22.27%). T6 recorded the highest mean value for panicle length (19.60cm – 27.50cm). T7 marked the highest mean values for number of productive tillers plantˉ1 (3 – 7), chlorophyll (mean: 1.28mg g−1) and leaf soluble protein content(mean: 8.82mg g−1) and leaf area index (mean: 0.71). In T5, more than 50 percent of positive segregants were recorded for grain weight panicleˉ1, number of filled grains panicleˉ1 and number of spikelets panicleˉ1. T6 recorded higher percentage of positive segregants for straw yield plantˉ1 whereas T7 recorded the highest positive segregants for 1000 grain weight. On the basis of dendrogram drawn the genotypes were grouped into four clusters. Cluster I -T4 and T6, cluster II-T2,T7, cluster III - T3, T5 and cluster IV - T1. Among the F2 segregants, Vaishak x Harsha (T6) performed well under upland condition whereas Vaishak x Vyttila 6 (T5) performed well under stress. The high yielding variety Vaishak released for uplands might have transferred its superior yield characters to the progeny. The transgressive segregants with favourable traits thus obtained can be effectively utilised for selecting superior genotypes combining drought tolerance and high yield.
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    ThesisItemOpen Access
    Genetic evaluation of F2 generation for yield and water stress tolerence in upland rice
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2018) Ivy Mary, Rajan; KAU; Lekha Rani, C
    The present study entitled “Genetic evaluation of F2 generation for yield and water stress tolerance in upland rice” was carried out in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2016-2018. The objective was to evaluate the pattern of variability in F2 for yield and yield contributing traits under upland and drought situations and to select superior segregants combining drought tolerance and high yield. The study material included four parents and three F2 populations selected from the Ph.D project entitled “Genetic analysis of drought tolerance in rice (Oryza sativa L.)”. The study comprised of two experiments .In experiment- I, the parents and F2 segregants were raised under rainfed upland condition exposed to natural stress. Among the F2 populations, Vaishak x Harsha (T6) recorded the highest mean grain yield plantˉ1 (38.45g – 48.15g) and lowest mean spikelet sterility (7.76%-21.18%). T6 recorded the highest mean values for number of productive tillers plantˉ1 (9 - 16) and leaf area index (mean: 1.67). Vaishak x Vyttila 6 (T5) recorded the highest mean values for number of spikelets panicleˉ1 (149 - 178), harvest index (44.88% – 48.15%) and proline content (mean: 0.29mg g−1). Thottacheera x Harsha (T7) recorded the highest mean values for chlorophyll(mean: 1.78mg g−1) and leaf soluble protein content (mean: 9.36mg g−1). The data obtained was subjected to transgressive segregation analysis and in T5 more than 50 percent positive segregants were observed for number of productive tillers plantˉ1, spikelet sterility, number of spikelets panicleˉ1 and panicle length. T6 recorded high percentage of positive segregants for number of productive tillersˉ1, grain yield plantˉ1, plant height and spikelet sterility. In T7 percentage of positive segregants were the highest for spikelet sterility and number of productive tillers plantˉ1. Dendrogram drawn on the basis of squared Euclidean distances classified the genotypes into four clusters. Cluster I -T1,T5,T6, cluster II- T4, cluster III -T2,T7 and cluster IV-T3. In experiment II, another set of parents and F2 segregants were grown under rainshelter imposing reproductive stage moisture stress. Irrigation was given at 20mm depth once in seven days from panicle initiation stage onwards. Among the F2 segregants, T5 recorded the highest mean values for grain yield plantˉ1 (11.80g – 18.88g), number of spikelets panicleˉ1 (128 - 174), grain weight panicleˉ1 (2.60g – 4.33g ), harvest index (42.44 % - 68.13%) and proline content (mean: 0.57) and the lowest for spikelet sterility (5.50% - 22.27%). T6 recorded the highest mean value for panicle length (19.60cm – 27.50cm). T7 marked the highest mean values for number of productive tillers plantˉ1 (3 – 7), chlorophyll (mean: 1.28mg g−1) and leaf soluble protein content(mean: 8.82mg g−1) and leaf area index (mean: 0.71). In T5, more than 50 percent of positive segregants were recorded for grain weight panicleˉ1, number of filled grains panicleˉ1 and number of spikelets panicleˉ1. T6 recorded higher percentage of positive segregants for straw yield plantˉ1 whereas T7 recorded the highest positive segregants for 1000 grain weight. On the basis of dendrogram drawn the genotypes were grouped into four clusters. Cluster I -T4 and T6, cluster II-T2,T7, cluster III - T3, T5 and cluster IV - T1. Among the F2 segregants, Vaishak x Harsha (T6) performed well under upland condition whereas Vaishak x Vyttila 6 (T5) performed well under stress. The high yielding variety Vaishak released for uplands might have transferred its superior yield characters to the progeny. The transgressive segregants with favourable traits thus obtained can be effectively utilised for selecting superior genotypes combining drought tolerance and high yield.
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    ThesisItemOpen Access
    Characterization and hybridization of nymphaea spp.
    (Department of Plant Breeding and Genetics, College of Agriiculture, Padannakkad, 2018) Manju, A; KAU; Namboodiri Raji, Vasudevan
    The investigation on "Characterization and hybridization in Nymphaea spp. " was undertaken in College of Agriculture, Padannakkad in the academic year 2016-18 with the objective to study the morphology, reproductive biology and to hybridize the seed forming Nymphaea sp. Based on the detailed survey in Northern Kerala, fourteen accessions were collected, of which ten were selected for detailed study. These consisted of two night blooming (Acc. 5 and Acc. 6) and eight day blooming types. Based on flower colour variation, there were accessions with different shades of pink (Acc.1, Acc. 2, Acc. 6, Acc. 9 and Acc. 10), blue violet (Acc. 3 and Acc. 8), white (Acc. 4 and Acc. 5) and violet (Acc.7). Growth and development pattern of leaves and flowers were similar in all the accessions. However, the ten accessions were distinct based on the leaf and floral characters. The leaves in all the accessions were simple orbicular with subpeltate lamina deeply cleft near to the petiole base. There was considerable variability in the colour, margin and tip of leaf lamina. The biometric characteristics of leaf such as length and width of lamina, length of petiole and longevity of leaf showed variability among the accessions, with Acc. 2 showing maximum length and longevity of the leaf. Flowers were pedicellate and complete with all the floral whorls in a spiral fashion on the floral axis. The process of opening and closing of flower repeated for four consecutive days, except in Acc. 6 where it continued till fifth day. Significant variation observed in flower morphology, anthesis and periodicity of flowering in all accessions with critical difference between day and night bloomers. The night blooming accessions showed superiority over day bloomers in the flower bud characters viz., length and circumference of flower bud and diameter of fully opened flower. The day bloomers differed from night bloomers in having shorter stigmatic appendages and stamens with terminal appendages. The night blooming accessions showed maximum duration for flower opening but low frequency of flowering compared to day bloomers. In general, all the accessions displayed strong protogyny except Acc. 5, where the phases of stigma receptivity and anther dehiscence were found overlapping. The pattern of anther dehiscence, duration of stigma receptivity and fragrance were favoring cross pollination in day blooming accessions. The adaptations such as overlapping of stigma receptivity and anther dehiscence in Acc. 5, simultaneous dehiscence of all anthers and absence of fragrance in both the night bloomers were pointing towards autogamy in these accessions. Pollen grains of all the ten accessions showed regular staining with 1% safranin. The in vitro pollen germination was maximum in 10% sucrose medium with seven accessions (viz., Acc. 1, Acc. 3, Acc. 4, Acc. 5, Acc. 6, Acc. 7 and Acc. 9) showing a remarkable increase in the pollen tube length. Only five accessions (Acc.3, Acc. 4, Acc. 5, Acc. 6, and Acc. 7) produced fruit and seeds in open condition with and without emasculation and no seed set under protected condition. The germinability and speed of seed germination was low without any treatments. Chemical scarification with different concentrations of H2SO4, GA3 and Ethrel showed an enhancement in seed germination, with maximum germination at a concentration of 100 ppm GA3 and 5% H2SO4. Besides rhizome propagation, other methods like leaf proliferation (Acc. 1 and Acc.7) and root tip proliferation (Acc. 5) were also observed. These alternative modes of propagation showed earliness in the formation of leaf, root and flowers compared to rhizome. Hybridization among five seed forming accessions showed success only among day bloomers (Ac. 3, Acc. 4 and Acc. 7) and with seed set in three crosses. Only the seedlings of cross 1 (Acc. 4 x Acc. 3) established successfully and produced flower within five months of seed germination. The initial evaluation of the morphological characters of hybrid progeny showed similarity with either of the parent for some characters. The colour of flower, color and shape of leaf were intermediate to both the parents and number of petals, stamens and carpels were unique to the hybrid progeny.
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    ThesisItemOpen Access
    Genotype x environment interaction in hedge lucerne (desmanthus virgatus (L.) willd.) for yield and quality
    (Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2018) Arun Chacko; KAU; Mareen Abraham
    The present work on “Genotype×Environment interaction in hedge lucerne (Desmanthus virgatus (L.) Willd.) for yield and quality” was carried out across four locations in Kerala viz., College of Agriculture, Vellayani, College of Horticulture, Thrissur, Krishi Vigyan Kendra, Kottarakkara and Regional Agricultural Research Station, Ambalavayal, Wayanad, during 2016-2018 with an objective to identify stable genotypes of Desmanthus virgatus in varied environments with respect to yield and quality. The eight genotypes of hedge lucerne viz., T1 (IC 345276), T2 (IC 343710), T3 (IC 89910), T4 (IC 261839), T5 (IC 90934), T6 (IC 421199), T7 (TNDV 1) and T8 (Thumburmuzhi local) were evaluated in a Randomized Block Design (RBD) with four replications over four locations during 2017-2018. Eberhart and Russell model (1966) was used to analyze the stability and adaptability of yield and yield related characters of these genotypes. Based on the environmental indices, COA, Vellayani and RARS, Ambalavayal was found most favourable for all of the characters, while COH, Thrissur was unfavourable for all the characters studied. In the pooled analysis of variance for evaluation of hedge lucerne over locations, significant differences among the genotypes and environments were noticed for all the characters studied, suggesting that genotypes interacted significantly with environments. Stability analysis revealed that the genotype T1 (IC 345276) was stable over all locations for different characters such as plant height, number of branches, green fodder yield, dry fodder yield, dry matter production and crude fibre. The genotype T4 (IC 261839) was stable for the characters number of branches, leaf to stem ratio and crude protein across the locations. The genotype T6 (IC 421199) was stable over locations for the characters viz., plant height, length of branches and number of leaves, while the genotype T7 (TNDV 1) was stable for leaf to stem ratio, number of pods and crude fibre. The genotypes T2 (IC 343710) and T8 (Thumburmuzhi local) were identified as stable genotypes for favourable environments. The genotype T2 (IC 343710) showed stable performance for the characters such as leaf to stem ratio, green fodder yield, dry fodder yield and crude fibre. The genotype T8 (Thumburmuzhi local) was stable in favourable environment for length of branches, green fodder yield, dry fodder yield and dry matter production. The genotype T5 (IC 90934) was found to be stable for the characters plant height and crude fibre in unfavourable environments. The present study revealed that the genotypes T1 (IC 345276), T4 (IC 261839), T6 (IC 421199) and T7 (TNDV 1) were stable over the four different locations viz., College of Agriculture, Vellayani, College of Horticulture, Thrissur, Krishi Vigyan Kendra, Kottarakkara and Regional Agricultural Research Station, Ambalavayal, Wayanad. The genotypes T2 (IC 343710) and T8 (Thumburmuzhi local) showed stable performance under favourable environments viz., College of Agriculture, Vellayani and Regional Agricultural Research Station, Ambalavayal, Wayanad, while the genotype T5 (IC 90934) was suitable for unfavourable environment i.e., College of Horticulture, Thrissur.