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ThesisItem Open Access Shelf life of irradiated rabbit meat underaerobic and vacuum packaging(Department of Livestock Products Technology, College of Veterinary and Animal Sciences, Mannuthy, 2009) Sonika, S; KAU; Kuttinarayanan, PPrevention of Food Adulteration Act (1954) as amended in 1998 has permitted irradiation at a dose rate of 2.5 to 4.5 kGy to control pathogenic microorganisms and to extend the shelf life of meat and meat products including poultry products. A study was conducted to evaluate the shelf life of irradiated rabbit meat under aerobic and vacuum packaging. The rabbit meat was prepared by slaughtering locally purchased rabbits under hygienic conditions and was packed in HDPE and PAPE packages at a rate of 120 g each. Half of the packets of aerobic and vacuum packaged samples were subjected to gamma radiation at 2.5 kGy at melting ice temperature and kept immediately at chiller temperature (1 to 4oC) and domestic refrigerator freezer (-6 to -8oC). Samples were analyzed for physical, physicochemical, microbiological, and organoleptic qualities on the day of preparation and on days 3, 5, 10, 15, 20, 25, 30, 40, 45, 50, 60, and 70 of storage or until spoilage, whichever was earlier. The samples were also analyzed for proximate composition on the day of preparation. The dressing percentage of the rabbits subjected to the study was 49.35 per cent. The keeping quality of the rabbit meat was 15 to 18, 17 to 19, 5 to 7 and 7 to 9 days in HDPE IR, PAPE IR, HDPE NR and PAPE NR respectively at chiller temperature. In freezer temperature it was significantly (P < 0.05) increased to 45 to 47, 47 to 49, 25 to 27 and 27 to 29 days in HDPE IR, PAPE IR, HDPE NR and PAPE NR respectively. Irradiation or packaging did not significantly (P < 0.05) affect fat, protein and ash composition but higher moisture percentage was observed in irradiated samples. Swelling of the collagen fibres was noticed in irradiated samples on histological examination. The physicochemical parameter, pH of irradiated and non-irradiated samples varied significantly (P < 0.05) on the day of preparation. On storage the pH values decreased uniformly. The WHC was significantly (P < 0.05) reduced in irradiated samples. The ability of the rabbit meat to retain its water decreased gradually on storage and a reduction of about 40 per cent could be noticed from the initial level. Drip loss was not significantly (P < 0.05) different on the day of preparation. Irradiation had a significant (P < 0.05) effect in enhancing the cooking loss whereas packaging had little effect. Both drip loss and cooking loss was increased significantly (P < 0.05) due to storage under chiller and freezer temperature. Irradiation had a significant (P < 0.05) role in increasing the TBARS value of rabbit meat. As the days of storage enhanced, the TBARS values were increased. Irradiation had a significant (P < 0.05) effect in reducing the TV in both type of packaging. As storage period enhanced, TV increased with significant (P < 0.05) changes among treatments. Irradiation had a beneficial effect on microbiological qualities of rabbit meat. There was a significant (P < 0.05) reduction of nearly three log in APC of irradiated meat from that of control. The irradiation of the samples both in HDPE and PAPE packaging significantly (P < 0.05) reduced the PC of meat on the day of preparation where as storage had significant (P < 0.05) effect in enhancing the microbial load of meat. The colour score was non-significantly (P < 0.05) higher in irradiated samples. The juiciness, tenderness and overall acceptability scores were improved significantly (P < 0.05) due to irradiation, where as flavour score was reduced. The sensory attributes were significantly (P < 0.05) reduced due to storage in all the treatment groups. The keeping quality of rabbit meat was significantly (P < 0.05) increased by irradiation both in chiller and freezer under different packaging. In addition irradiation could effectively control food borne illness by destroying the major pathogenic organism without affecting the sensory and nutritional quality of the product. Considering these advantages it can be recommended that packaging the meat in PAPE packages followed by low dose gamma irradiation and maintaining the cold-chain contribute to extended storage life of rabbit meat.ThesisItem Open Access Waste management system evaluation in commercial dairy farms(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2009) Sany Thomas; KAU; Joseph MathewThe present study on “Waste management system evaluation in commercial dairy farms” was conducted to assess the usefulness of the different waste management methods adopted in dairy farms of Kerala. Study area comprised of Thrissur, Malappuram and Ernakulam districts and adjoining area of central and northern Kerala. Forty five dairy farms were identified and visited in and their profile of the study area .Data regarding general outlay of the farms selected management practices in the farm, livestock details and existing waste management methods in the farms were collected and studied. The dairy farms under study were classified based on the animal holding capacity as those with less than six animals (class 1), 6-20 animals (class 2), 21-50 animals (class 3)and 51-100 animals (class 4), and above 100 animals (class 5). Among the 45 farms under study, four per cent belonged to class 1, forty nine per cent belonged to class 2, thirty five belonged to class 3, six per cent farms came under the classes 4 and 5.There was no commercialization of waste management methods adopted by the farms. The majority farms had dung pit as waste disposal method and no regularity in dung removal from pit, it was upon demand. Regarding the soil quality, there was no significant difference in the soil nutrients quantity between different groups of farms. Regarding water quality, the water samples collected from the nearby water bodies were inferior in quality in terms of microbiology and BOD. Air quality stands below the permitted standards in all groups of farms. Intervention by the government, local bodies and scientific institutions must be made in this area supporting the farmers to develop awareness in this serious issue and also providing necessary technical and financial support for the farmers to construct proper waste management systems such as compost unit as well as biogas plants because they provide a support for the most efficient waste management system in a dairy farm proving that waste is wealthThesisItem Open Access Efficay of probiotic and ascorbic acid in alleviating summer stress in growing broiler rabbits(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2010) Smitha, S; KAU; Kannan, AThe research work was conducted to study the efficacy of probiotic (Lactobacillus casei) and ascorbic acid in alleviating summer stress in growing broiler rabbits. The study was done in the summer season from March to May. The growth performance of rabbits in summer, their physiological response to stress and the cost effectiveness of supplementing probiotic, Lactobacillus casei and ascorbic acid were studied. Twenty four weaned New Zealand White rabbits were randomly selected from Rabbit unit at Krishi Vigyan Kendra, Kerala Agricultural University, Vellanikkara were utilized for the study. They were divided into four groups of six animals each. The treatments were as follows: Treatment I (T1) - Ascorbic acid (Merck) at the rate of 200 mg per kg feed was given along with the basal diet, Treatment - 2 (T2) - Probiotic, Lactobacillus casei (Unique Biotech) containing 106 colony forming units per gram of feed was given along with the basal diet, Treatment - 3(T3) - Probiotic and Ascorbic acid at the same rate as in T2 and T3 were given along with the basal diet and Treatment - 4 (T4) - Rabbits fed with basal diet alone. The various climatic parameters studied were maximum and minimum temperature and relative humidity (morning and afternoon) in macro climate and maximum and minimum temperature, relative humidity (morning and afternoon), dry bulb temperature (morning and afternoon) and relative humidity (morning and afternoon) in micro climate. The production parameters recorded were weekly body weight, daily feed intake, average daily weight gain and feed efficiency. The physiological parameters studied were weekly respiration rate, weekly rectal temperature and monthly cortisol (faecal and serum) values (twice a month for three months). Disease incidence and mortality during the period was recorded. Cost effectiveness of supplementing probiotic and ascorbic acid was determined. The mean monthly temperature humidity index values suggested that animals were under moderate heat stress in the first (28.45±0.16) and the second month (28.50±0.22), while no stress prevailed in the third month (26.83±0.21). By one way Analysis of variance it was found that supplementation of probiotic, Lactobacillus casei and ascorbic acid had a significant effect in alleviating summer stress in rabbits. The animals in the T1 group showed significantly (P<0.05) higher overall mean daily body weight gain, overall mean feed efficiency and significantly (P<0.05) lower overall mean respiration rate and faecal cortisol level compared to T4 animals. The rabbits in T2 treatment showed significantly higher (P<0.05) final body weight, overall mean daily body weight gain, overall mean feed efficiency and significantly (P<0.05) lower overall mean respiration rate compared to T4. The rabbits in the T3 group showed significantly higher (P<0.05) final body weight, overall mean daily body weight gain, overall mean feed efficiency and significantly (P<0.05) lower overall mean faecal cortisol level compared to T4. There was no significant difference (P>0.05) between the treatments in mean feed intake, rectal temperature and serum cortisol. No disease incidence or mortality was observed during the experimental period. Supplementation of probiotic and ascorbic acid was found to be efficient in reducing the production economics. Hence it was concluded that supplementation of probiotic, Lactobacillus casei at the rate of 106 cfu per g of feed and ascorbic acid at the rate of 200 mg per kg feed in combination was found to be most effective and economic in alleviating summer stress in growing broiler rabbits.ThesisItem Unknown Androgen mediated behavior of sambar deer stags (Cervus unicolor) during rut season(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2010) Vishnu Savanth, V; KAU; Saseendran, P CThe study was carried out at the State Museum and Zoo, Thrissur, Kerala, India for a period of four months from 11th June to 11th October, 2009. There were a total of 70 sambar deer in the enclosure, during the commencement of the study, of which 22 were males including 16 adult stags. A total of 318 hours of observation was involved in the study spanning over four months and each animal received about 53 hours of observation. Top three stags in the rut stage, on a chronological order of dominance namely H1, H2 and H3 were selected. Three more superior stags in late stages of velvet growth were selected and were named V1, V2 and V3 as per descending order of dominance. Hence, a total of six animals were selected for the study. Behavioral scores were allotted on the basis of the observations recorded on the ethogram and with the help of a standard score chart. Stag H1 maintained a behavioral score of 42±11.73 during the hard antler phase whereas during the velvet phase it slipped to 9.66±2.25. The behavioral score of stag H2 was 42.85±12.58 during the hard antler stage and it reduced to 12.6±2.3 during the velvet period. Stag H3 had a behavioral score of 44±14 during the rut season; it became 12.6±3.13 during the velvet stage. The behavioral score of stag V1 was 23.16±12.84 during the velvet stage, but as it entered the rut season, its score shot up to 51.81±5.54. Stag V2 had a behavioral score of 24.85±13.83 during the velvet phase, as it shifted to hard antler stage, the score increased to 51.9±5.38. The behavioral score of V3 was 30.33±14.76 during the velvet antler stage, the shift to the rut took up the score to 53.75±4.83. Fecal testosterone level was measured by RIA on a weekly basis from all six stags. The stag H1 possessed the largest harem followed by H2 and H3 with a membership of 17, 13 and 07 individuals respectively till they retained the hard antlers. As V1, V2 and V3 entered the rut season, V1 collected the largest harem with 19, followed by V3 with 14, and V2 not lagging far behind with 13 members. The stag H1 maintained a testosterone level of 14.66±2.30 ng g-1 of dry feces when it was in rut stage. The testosterone level after the antler casting in the seventh week maintained a low profile of 7.85± 2.32 ng g-1 of dry. The testosterone level of stag H2 was 14.07±0.54 ng g-1 of dry feces during the hard antler phase and its decline by the eight week led to the antler casting. The testosterone level then was at a level of 9.12± 2.40 ng g-1 of dry feces. Stag H3 had testosterone levels of 14.85±1.17 ng g-1 of dry feces before casting the antler. The casting was followed by testosterone levels of 9.56±1.94 ng g-1 of dry feces. Stag V1 had testosterone levels of 13.52±1.82 ng g-1 of dry feces in the velvet stage. After velvet shedding, the stag had hormone levels of 18.65±1.20 ng g-1 of dry feces. V2 maintained testosterone levels of 12.45±0.91 ng g-1 of dry feces during the velvet stage. Velvet shedding was accompanied by an increase in testosterone level to 18.77±1.03 ng g-1 of dry feces. The stag V3 had the hormone levels at 12.32±1.18 ng g-1 of dry feces before casting the velvet. The testosterone level after the velvet casting was 18.72±0.79 ng g-1 of dry feces. The stags in the rut season were found to have a significantly higher testosterone concentration in comparison with the velvet growth stage stags. The study also reveals that, it is the sudden dip in the testosterone concentration which causes the antler casting. Velvet shedding was preceded by an increase in the testosterone levels emphasizing its role. The increasing behavioral scores were also accompanied by higher levels of testosterone. Statistical analysis to correlate the testosterone levels of all the stags during both hard antler and velvet stage to the behavioral scores showed a significant and positive correlation ( ρ = 0.875, p < 0.01 ). Statistical analysis of the testosterone level and behavioral score during the hard antler stage alone also showed positive and significant correlation ( ρ = 0.791, p < 0.01 ). The correlation between the testosterone level and the behavioral score was significant and positive during the velvet stage as well ( ρ = 0.805, p < 0.01 ).ThesisItem Unknown Evolving low cost ration for commercial broiler rabbit production(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2006) Biya Ann, Joseph; KAU; Kannan, AA study was conducted to document the present rabbit farming scenario in Thrissur district and to evolve a low cost ration for rabbits utilizing concentrates and vegetable cuttings in different ratios. The documentation was conducted on thirty rabbit farmers using a well-designed questionnaire and personal interview. The socio-economic status of the rabbit farmers and management practices were studied. The composite feed samples fed to rabbits were analysed. The rabbit farmers mostly lived in a nuclear family system and were employed either in public sector or owned business. The reason for engaging in rabbit rearing was for additional income generated and for the value of keeping rabbits as pets. Most of the farmers had only 1-6 months experience and the responsibility of rearing rested with wife and children. New Zealand White was the preferred breed and the rabbits were mostly obtained from local rabbit farmers. Most farmers maintained herd strength of less than 20. A mixture of concentrate and locally available leaves and grasses was the major feeding method with a feeding frequency of twice a day and most rabbits were fed 100-125 g ration daily. The crude protein, crude fibre and ether extract of composite rabbit feed was 15.48%, 14.98% and 1.74% respectively. Pan watering was the common method of watering. Cage system was the preferred type of housing with a cage size of 2-4 sq.ft. and 4-6 sq.ft. Majority of the cages were located adjoining the house. Most rabbits were sold at 2 months of age at Rs. 100-150 per pair. Digestive disorders were the frequently occurring disease and rabbits were mostly treated by veterinary doctors. Use of nest box, manure disposal and daily cage cleaning were the commonly adopted scientific management practices. Thirty weaned New Zealand White rabbits were selected and utilized for the study. They were divided into five groups of six animals each and allotted to one of the following treatments: T1 – Rabbits fed with the ration (100 per cent concentrate) provided by the Rabbit Research Station, Mannuthy (Control). T2 – Rabbits fed with 75 per cent concentrate and 25 per cent vegetable cuttings on Dry Matter (DM) basis. T3 - Rabbits fed with 50 per cent concentrate and 50 per cent vegetable cuttings on DM basis. T4 - Rabbits fed with 25 per cent concentrate and 75 per cent vegetable cuttings on DM basis. T5 - Rabbits fed with 100 per cent vegetable cuttings on DM basis. The parameters like fortnightly body weight, fortnightly body weight gain, average daily gain, average daily feed intake on dry and fresh matter basis, feed conversion efficiency, proximate analysis of concentrate and vegetable cuttings, occurrence of disease, carcass traits and economics of production showed significant difference between the treatments. T2 had the highest body weight among all treatments whereas T1 and T3 showed no significant weight difference and T5 had the least weight. A similar trend was seen in average fortnightly weight gain and average daily gain, with T2 having the highest gain and T5 the least gain. The daily feed intake had a linear increase from first to the sixth fortnight in all the treatment groups. The feed conversion efficiency (FCE) increases with increase in the percentage inclusion of vegetable cuttings on DM basis. Vegetable cuttings had a higher percentage moisture, crude fibre and total ash (79.63, 17.08 and 9.58) than concentrate (10.75, 4.09 and 3.57) but lower crude fat (1.75) and nitrogen free extract (50.64). The percentage crude protein content was almost similar in both concentrate (21.82) and vegetable cuttings (20.97). Digestive disorder was the commonly observed disease closely followed by mange. The dressing percentage varies from 45.31 in T5 to 52.27 in T2. The cost of production of one kg of rabbit meat ranges from Rs. 47.52 in T1 to Rs. 8.72 in T5. T2 has a higher weight and feed conversion efficiency, but the cost of production of Rs. 41.56 is high compared to T3, which is on par with T1 in weight gain and lower by Rs. 13.92 in its production cost. Hence rearing rabbits on a combination of 50% concentrate and 50% vegetable cuttings can be recommended for commercial broiler rabbit production.ThesisItem Open Access Management of captive young elephants in Kerala(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2006) Acty, George; KAU; Anil, K SThe present study was undertaken to suggest suitable recommendations in the management of captive young elephants of Kerala after assessing the existing management, feeding and training practices through survey method. The credibility of information collected was cross checked with physical verification. Data was collected from the owners’ house holds and offices and sites where animal was tethered by personal interviews during March to August 2006. The observations made were on routine, housing feeding, diseases and health management, behaviour and temperament and record keeping. From the study, it was evident that majority of the elephant owners, first and second mahouts belonged to middle age groups. Even though none of the owners received training, half of the owners were richly experienced in elephant management. Majority of the owners and mahouts selected elephant rearing as hereditary business or job. Injury was a major constraint in case of mahouts. The young animals were divided in to three groups based on their age as 0- 2yrs (Group I), 2-6yrs (Group II) and 6-15yrs (Group III). Group II and III received more watering, wetting and bathing. Frequency of wetting and watering was highest in summer season. Actual training starts at 6 years of age and train the elephants for various purposes. It was evident that young elephants are fed above the nutritional requirements and kept idle for most of the time in the year. Boredom was found to be a factor for behavioural anomalies. Major ailments in young ones was diarrhoea were as impaction in adults. There was record keeping only in organized elephant care facilities.ThesisItem Open Access Techno economic analysis of rabbit farming in Kamakshy panchayath of Idukki district(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2007) Chitra, R Nair; KAU; Saseendran, P CThe study was conducted to evaluate the success rate of rabbit farming in Idukki district, where the rabbit population is highest (38,433) in the state. Fifty rabbit units were selected at random and classified based on the number of doe units reared by them as small(1-10 doe unit), medium (11-20 doe unit) and large (>20 doe unit) to evaluate the farmers profile and managemental practices. Macro and microclimatological data of the study area were recorded during the study period. Monthly mortality of rabbits, breeding and monthly production of kits were also recorded. The feed ingredients fed to rabbits were analyzed. Mineral status, bio chemical parameters and lipid profile of serum was checked. Rainy season and transition period to rainy season found to have a pronounced impact on the mortality of rabbits in Kamakshy panchayath. The rabbit farmers here were not breeding their rabbits during the rainy season. The rabbit farmers mostly lived in nuclear family system and were having rabbit farming as their subsidiary occupation. Both middle aged (31-50 years) people and youngsters (<30 years) were mainly involved. In Kamakshy panchayath rabbit farming serve as an additional income for the small and medium farmers and as a means of livelihood security as far as the the large farmers are concerned. Majority of them were educated and having 5-10 years of experience.A combination of local grasses and kitchen waste were the predominant feeding pattern among small farmer groups. Cage system was the preferred type of housing with a cage size of 1-2 sq.ft. and 2-4 sq.ft. Cages were located adjoining the house, 1-15 ft to > 15 ft away from the house. Housing cost found to be very cheap in Kamakshy panchayath. Rabbits were sold predominantly on live weight basis, above two months of age in all the three farmer groups and were sold at less than Rs.100 per kg body weight. Pasteurellosis and Coccidiosis were the frequently occurring disease and this account for the major share of mortality among the rabbits. Majority of the small and medium farmers were doing treatment with the help of experienced farmers. The serum parameters like total proteins, albumin, globulin, calcium, phosphorous, triglycerides, cholesterol, HDL, LDL, proximate analysis of feed ingredients, slaughter studies, chemical analysis of meat and economic analysis of rabbit farming showed significant difference between groups. Azolla had a higher crude protein (18.68±0.32 Vs 12.62±0.32) and lower crude fibre (13.03±0.26 Vs 18.38±0.38) content compared to mixed grass. The protein content of azolla is comparable to the concentrate feed for rabbits and fibre content of mixed grass is good to prevent the digestive disorders. The dressing percentage varies from 41.11 in small to 51.3 in large farmer groups. Chemical analysis of meat revealed the highest value for crude protein content in Longissmus Dorsi (LD) muscle (20.79±0.50) and hind leg (20.47±0.49). The profit in rabbit farming from a single doe among the three farmer groups were calculated. Constraints in rabbit farming were studied and possible suggestions were made. Cogg- Douglas production function for rabbit farming in Kamakshy panchayath has given an average return to scale of 1.89. The profit per month from a single doe unit in small, medium and large farmers found to be Rs.117.13, Rs.133.73 and Rs.174.38 respectively. Cost for cages (Rs.38.87) and feed (Rs.449.93/year) which form the lions share in rabbit farming is very least among small farmer group. Thus this small scale backyard rabbitry can be a useful enterprise for providing supplementary income to improve the socio-economic conditions of the rural and urban poor and a large scale rabbitry can be recommended as an enterprise for livelihood security.ThesisItem Open Access Management protocol for averting drop of milk production in dairy cattle during summer(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2009) Nisanth, P; KAU; Kannan, AA study was conducted to assess the effect of feeding management on milk production and metabolic profile of crossbred cows in mid lactation. Twelve healthy crossbred cows having a peak yield of minimum eight litres in the previous lactation, were selected as the experimental animals. The animals were divided into two groups of six each as uniformly as possible with regard to age, milk yield and parity and were randomly allotted to two dietary treatments, T1 consisting of basal concentrate mixture and green grass as roughages and T2 consisting basal concentrate mixture, green grass as roughage of which 1/3rd of the concentrate and roughage is fed during the day time and rest in the evening and early morning with water availability at all times. Daily maximum temperature, minimum temperature and relative humidity were recorded inside the shed using maximum and minimum thermometer to quantify the microenvironment prevalent around the animals in both the treatment groups. Physiological parameters like respiration rate and rectal temperature were measured to give an immediate response to the climatic stress and consequently the level of comfort to the animal. Individual records of daily intakes of concentrate and roughage, daily milk production and fortnightly body weight were maintained through out the experiment. Milk samples were collected at fortnightly intervals and were analysed for milk fat, total solids and solids not fat. Blood was collected at fortnight intervals for estimating haemoglobin, erythrocyte sedimentation rate, packed cell volume, plasma glucose and cortisol. The ambient temperature prevailed during the study period was higher than what was reported as comfortable or ideal temperature for better livestock production and the excess humidity and temperature levels recorded in the present study at Mannuthy could be classified as humid and hot. The average rectal temperature during morning and afternoon between T1 and T2 did not differ significantly. The mean values clearly indicated that high ambient temperature increased the respiratory rate in the afternoon. The respiration rate for morning and afternoon recording was not found to be differed between T1 and T2. Average body weight of animals revealed no significant difference for the both groups during all the fortnight studied. The average daily dry matter intake linearly increased as the lactation progressed in both the groups. Dry matter intake by the animals of T2 was comparatively better than the animals of T1 with a significant increase (P<0.05) during the fourth and seventh fortnight. Scrutiny of the data on milk production during first 120 days of lactation further revealed that comparatively higher milk yield and better persistency was observed in T2. There was no significant difference in any of the milk composition parameters between the treatments. The haematological parameters such as haemoglobin, packed cell volume, erythrocyte sedimentation rate and plasma glucose estimated at the fortnight intervals were not significantly affected by the two dietary treatments. On statistical analysis a significant difference (P< 0.05) in plasma cortisol was obtained between the two groups during the first and second fortnights. The overall average plasma cortisol level was significantly higher in the T1 in comparison to T2. From the overall results obtained in the present study it could be concluded that the night time feeding of the animals during summer season has improved total milk production and helped to maintain the higher milk yield as well as persistency of milk production in lactating crossbred cows and found that there was some long-term effect on performance of evening fed cows.