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ThesisItem Open Access Divergence studies in pumpkin (Cucurbita moschata Pair)(Department of Olericulture, College of Horticulture, Vellanikkara, 1989) Suresh Babu, V; KAU; Gopalakrishnan, T RThe present investigation on "Divergence studies in pumpkin (Cucurbita moschat a Poir ) was conducted at the College of Horticulture , Vellanikkara, Trissur during June 1988 - Marc h 1989. Seventy one pumpkin genotypes collected from different part of India and abroad were utilized for the study. The extent of variability and divergence among 50 selected genotypes were assessed and grouped into 5 clusters based on Mahalanobis D2 statistic . Cluster I, II, III, I V and V contained 2, 7, 9, 12 and 20 genotypes respectively y . Intercluster distance was maximum between clusters I and II and was minimum between clusters III and V . Cluster I showed maximum average intercluster distance with any other cluster. Screening 71 genotypes for resistance/ tolerance to pumpkin mosaic and yellow vein mosaic diseases revealed that all genotypes except C M 214 were susceptible. Artificial inoculation studies confirmed immunity of C M 214 (Nigerian Iocal ) to pumpkin mosaic virus and yellow vein mosaic virus .ThesisItem Open Access Studies on fertility status of rubber growing soils of Palakkad district(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1991) Mohanan, V; KAU; Nazeem, P AThesisItem Open Access Standardization of growing media for dendrobiums (Qendkobium dpp.)(Department of Horticulture, Pomology & Floriculture and Landscaping, College of Horticulture, Vellanikkara, 1989) Anitha, Paul C; Rajeevan, P KThesisItem Open Access Studies on growth and bearing habits of certain varieties of bhendi (Abelmoschus esculentus (L.) Moench.)with special reference to fruit maturity and quality(Regional post-graduate training centre, Agricultural college and research institute, Coimbatore., 1964) Ravindra, K; KAUThesisItem Open Access Study on the effect of foliar application of urea and planofix on the growth, yield and quality of ginger varieties (Zingiber officinale Roscoe)(Department of Horticulture, College of Agriculture Bhubaneswar, 1975) Sreekandan, Nair; Das, R CThesisItem Open Access Investigations on intervarietal F2 hybrids in cowpea(Department of Agricultural Botany, College of Horticulture Vellanikkara, Trichur, 1981) Sumathikutty Amma, B; KAU; Narayanan Namboodiri, K MCcwpea (Vlgna ungiculata) Is the major pulse crop cultivated in Kerala where the average yield of pulses in general and Cowpea in particular is very low even under modern agronomic practices* This low productivity is mainly due to the absence of high yielding varieties suited for the different agro-climatic conditions. The varietal requirements in Cowpea, in the state vary because of the highly contrasting system of cultivation and this compels Cowpea breeders to evolve varieties of different combinations of plant, pod and seed characters, This is possible by producing different varieties combining the desirable attributes required for the diverse situations by suitable means. One of the ways by which, this can be achieved is through combination breeding in which desirable genes are pooled frcra different sources* In a previous study conducted in the Department of Agricultural Botany, the 202 genotypes v/ere grouped into 17 distinct clusters. Representing 15 clusters, 15 varieties were chosen for intervarietal hybridisation and their F^'s evaluated during 1979-80, Seeds collected from the plants of the 16 Intervariota] crosses along with their parents were used for the study. Two families In each of the 16 crosses were studied along with their respective parents for the pattern of inheritance of 15 economic characters. Most of the characters were found to be inherited as quantitative characters controlled by either polygenes or by a few major genes with their action being suitably modified by minor genes. The crosses 5 x 15 (g p .p l s . 139 x p .118) and 6 X 8 (Red Seeded Selection X Kolingipayar) were identified as suitable ones for grain - production and also for using as dual purpose Cowpea culture since they might throw segregants best suited to those purposes. For the specialised system of Cot/pea culture practised in summer rice fallows exclusively for vegetable purpose, the crosses 14 X 16 (Pannithodan-early X Kolingipayarwhite), 17 X 16 (Mancheri-black X Kolingipayar^hite) and 10 X 6 (IC. 20729 X Red Seeded Selection) appeared to be the Ideal ones*ThesisItem Open Access Molecular characterization of piper species usiing RAPD technique(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 2002) Murugan, C; KAU; Sujatha, V SThe study on "Molecular characterization of Piper species using RAPD techniques" was conducted in the Department of Plantation Crops and Spices and CPBMB, College of Horticulture, Vellanikkara, Thrissur from December, 1999 to August, 2001. Material included 13 species/accessions of the genus Piper including Piper nigrutn Linn. These were analysed with 20 selected oligomer decamer primers using PCR technique and agarose gel electrophoresis. The selection of ideal DNA isolation technique was done based on observations of purity of bands, quality of the DNA bands and quantity of DNA recovered. The protocol of Edwards et al. (1991) was selected for DNA isolation with additional step of chloroform-isoamyl alcohol treatment. Fifty decamer primers were screened using DNA. of P. nigrum and 20 were selected based on the number of bands produced, reproducibility of the bands and distinctness of the RAPD profile. RAPD profile of the 13 species of Piper were compared for genetic similarity index (using Jaccard's coefficient), with all 20 selected primers separately. Pooled similarity of the twenty primers put together was found out for 9 species which gave amplification for all the 20 selected primers. The results of pooled analysis was subjected to cluster analysis (SAl-IN) and phenetic dcndrogram was constructed employing UPGMA. Four clusters were obtained, comprising of two accessions of P. nigrum In the first, two accessions of P. longum in the second, P. colubrinutn and P. attenuatum forming the third cluster and P. chaba, P. belle and P. arboreum forming the fourth.ThesisItem Open Access Evaluation of curry leaf (Murraya koenigii L.) accessions for yield and quality(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 2019) Anila, Peter; KAU; Krishnakumary, KCurry leaf (Murraya koenigii L.) is an important perennial herbal spice widely used for culinary purpose in India for centuries and the leaves valued for its characteristics flavour and aroma. Leaves possess medicinal and neutraceutical properties due to the presence of vitamins, minerals and phytochemicals. In Kerala, cultivation is limited to landraces which are often reported to be low in quality. Identification of promising types with high yield and quality will pave way for promoting large scale cultivation in Kerala. The present study entitled “Evaluation of curry leaf (Murraya koenigii L.) accessions for yield and quality” was undertaken with objectives of morphological and biochemical characterization of available accessions and standardization of vegetative propagation. accessions of curry leaf maintained by the Department of Vegetable Science, College of Horticulture, Vellanikkara along with the released variety Suvasini were used as the experimental material. Among the accessions, wide variability was recorded for fifteen quantitative and five qualitative morphological characters studied. Cluster analysis was performed and superior accessions were selected and those were subjected to biochemical analysis. Among the accessions, 76 % were having green midrib and petiole whereas 24 % with light red pink. Leaf colour was found as dark green in 19 % of accessions and the remaining accessions were green in colour. Only two leaflet shape were noticed with 19 % having lanceolate shape. The plant height varied from 2.23 m (MK 107) to 5.21m (MK 103) whereas branching height ranged from 31.25 cm (MK 125) to 67.51 cm (MK 122). Accession MK 118 recorded highest canopy spread in N-S direction (4.57 m) while MK 142 recorded in E-W direction (4.78 m). Considerable variability was observed in number of primary, secondary and tertiary branches and more than 40 numbers of tertiary branches were recorded in five accessions namely MK 107, MK 118, MK 126, MK 142 and MK 160. The leaf length ranged from 14.71 cm (MK 122) to 21.81 cm (MK 126) whereas maximum number of leaflets (22 leaflets) were observed in three accessions viz., MK 126, MK 142 and Suvasini with the largest leaf area of 66.46 cm2 in MK 142. Curry leaf was harvested at four months interval from June to March. Harvesting during June- July months in the rainy season recorded the highest leaf yield per plant. Leaf yield of more than 5 kg per plant was obtained in four accessions namely, MK 107, MK 126, MK 142 and MK 160. Aroma and flavour are the important parameters in curry leaf and based on sensory evaluation, eight accessions were identified as superior with a mean sensory score of above 7, with the highest score of 7.40 and 7.33 for flavour and aroma in MK 126. Correlation analysis revealed significant and positive correlation of yield with number of primary, secondary and tertiary branches, canopy spread in N-S and E-W direction, number of leaflets, and leaf area. Based on cluster analysis, all the thirty accessions under the study were grouped into twelve clusters and ten accessions from superior clusters were selected for biochemical analysis. Oil recovery was higher in dry leaves than in fresh whereas antioxidant activity and beta carotene content were higher in fresh leaves. Highest essential oil recovery was recorded in dried samples of accession MK 186 (1.09 %) and maximum content of crude fibre was recorded in two accessions namely MK 118 (11.61 %) and MK 186 (11.65 %). Accession MK 126 recorded the highest content of carbohydrate (15.99 %) and protein (6.00 %). Highest content of total phenol (0.31 %) and antioxidant capacity (3.45) was recorded in MK 105. The beta carotene content was found higher (˃ 8.80 mg 100g-1) in three accessions namely, MK 105, MK 142 and MK 186. Considering yield and quality MK 142 and MK 126 were identified as most promising types followed by MK 105 and MK 186. Standardization of vegetative propagation was done in Suvasini with softwood, semihardwood and hardwood types of cuttings, two growth regulators (IBA and NAA) at different concentrations of 1000ppm, 2000ppm and their combinations during rainy, winter and summer season. Even though success rate of vegetative propagation through stem cuttings was very low, hardwood cuttings treated with highest concentration of growth regulator (2000ppm IBA+2000ppm NAA) resulted in 3.3 % of establishment during rainy seasonThesisItem Open Access Collection and evaluation of Wayanad turmeric (curcuma longa l.) for yield and quality(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 2019) Anu, T S; KAU; Jalaja Menon, STurmeric (Curcuma longa L.) is a rhizomatous perennial herb and an important sacred spice of India. It is used as a spice, colourant and an aromatic stimulant in several medicinal preparations. Wayanad local turmeric is reported to contain an average of 9.4 per cent curcumin. Due to the advent of improved varieties, the traditional cultivars of Wayanad are eroding. Keeping in view of this fact the study was undertaken to evaluate the local Wayanad accessions for yield and quality. Twenty six accessions of traditional ecotypes were collected from three taluks of Manathavady, Vythiri and Sultanbathery of Wayanad district through mass publicity. Seventeen accessions having sufficient planting materials for replicated trial were selected for field trial along with three improved varieties, Sona, Kanthi and Prathibha as checks. The crop was raised under the partial shade of 54.29 per cent in the coconut farm of the Department of Plantation crops and spices in randomized block design. Morphological observations were taken at 150 days after planting. Wayanad local turmeric accessions performed better than the check in all the biometric characters except length of the leaf. The plant height was highest for WCL 23 and girth was highest for WCL 3. The number of leaves on the main tiller was significantly high in WCL 15 and 16 while number of tillers per clump was highest in WCL 22. Leaf with maximum length and width were recorded in Kanthi and WCL 16 respectively. Horizontal rhizome branching was observed in the Prathibha , 3, 13, 16, 17, 24 and 25. The variety Prathibha and accessions 16, 17, 20, 21, 24 and 25 showed plumpy rhizomes. Compact rhizomes were observed in 8, 9, 13, 14,15, 16, 19, 20 and 25. The inner core colour of the rhizome was light yellow in accession WCL 24 and reddish yellow Colour was observed in Prathibha, Kanthi and WCL 3. All other accessions had orange inner core colour. The accessions and varieties were categorized based on percent disease index. The variety Prathibha and accessions 3, 13, 14, 15, 16 and 25 were highly resistant to leaf blotch. Accession WCL 5 was highly susceptible to leaf blotch. All the accessions were resistant in disease reaction to leaf spot except WCL 19, which was moderately resistant. The incidence of pests like lema beetle and shoot borer were observed in all accessions while leaf roller incidence was noticed only in Prathibha, WCL 5, WCL 17 and WCL 24. Fresh rhizome yield per plant was significantly high in accessions WCL 24 (350.33 g) and WCL 23 (350.08 g) and were on par with Kanthi (335.60 g) and WCL 25 (274.66 g). Accession WCL 25 was superior in fresh rhizome yield per plot and per hectare (32.32 t ha-1). Accessions WCL 23 and WCL 25 performed over and above the checks in terms of fresh yield, curcumin content and curcumin yield. Curcumin content was high for WCL 5 (10.18 %) followed by WCL 25 (9.27 %) and WCL 26 (9.31 %). The accession WCL 5 was highly susceptible to leaf blotch and curcumin yield (286.06 kg ha-1) was low. The fresh rhizome yield was highest in WCL 25 (32.32 t ha-1). Curcumin yield was highest for WCL 25 (551.40 kg ha-1) and accessions WCL 20, WCL 23, and WCL 26 were on par with WCL 25. Oleoresin content was significantly high in accessions WCL 3 (19.90 %) and WCL 22 (19.57 %). Essential oil content was highest for Sona (4.00 %) and Prathibha (4.00 %). Selection index was also calculated from fresh rhizome yield per clump curcumin content, dry rhizome recovery and disease reaction. Accessions 3, 13, 14, 15, 16, 23 and 25 had a highest score of 12 and these were identified as superior accessions which can be used for further crop improvement programme.
