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Open Access
Evaluvation of China aster [Callistephus chinensis (L.) Nees.] for cultivation in tropical plains
(Department of Floriculture and Landscaping, College of Horticulture, Vellanikkara, 2019) Alfin, Santhosh; KAU; Anupama, T V
China aster [Callistephus chinenesis (L.) Nees.] is one of the most important annual flower crops grown in India for cut flower as well as loose flower purposes. It ranks third next to chrysanthemum and marigold among the annual flowers and has gained popularity due to ease of cultivation, diversity of colours and good vase life. It is also used for bedding purpose in landscapes and as pot plants. In Kerala, the demand for annual flower crops is on the rise, however the cultivation is limited. China aster due to its hardy nature can be grown as a pure crop as well as intercrop in coconut gardens. The investigation entitled ‘Evaluation of China aster [Callistephus chinenesis (L.) Nees.] for cultivation in tropical plains’ was conducted at the Department of Floriculture and Landscaping during 2018-2019 with an objective to evaluate the performance of China aster for cultivation in tropical plains. Ten varieties of China aster viz. Arka Kamini, Arka Shashank, Arka Archana, Phule Ganesh White, Phule Ganesh Violet, Phule Ganesh Pink, Phule Ganesh Purple, AAC-1, Local Pink and Mat White were evaluated for vegetative, floral and post harvest characters. The varieties showed significant variation for vegetative characters. In all the varieties, leaf dentation was present and leaf shape was ovate for all varieties except Arka Shashank which had linear leaf shape. Stem colouration and leaf mid rib colouration were absent in varieties such as Arka Shashank, Arka Archana, Phule Ganesh White and Mat White and the rest of the six varieties showed the presence of purplish stem and mid rib colouration. The variety Phule Ganesh Pink was superior in plant height (68.86 cm) at 3 months after planting. The characters like plant spread (50.08 cm), number of leaves (56.48), number of primary branches (14.25) and stem girth (11.09 cm) were also highest in variety Phule Ganesh Pink. Leaf area was the highest in variety Phule Ganesh White (34.31 cm2). The variety Mat White was significantly inferior for all the vegetative characters observed. Floral characters showed significant variation among the varieties. Powderpuff flower type was observed in Arka Shashank and all the other varieties had semi-double type flower. Days to bud initiation was minimum in variety Arka Shashank (44.40 days) and maximum in Phule Ganesh White (73.67 days). The same trend was observed for days to complete flower opening and days to 50 per cent flowering. The variety Local Pink had the highest duration of flowering (62.40 days) and variety Mat White (45.20 days) had the lowest duration of flowering. The variety Arka Shashank produced highest number of flowers per plant (20.20). The flower stalk length (21.13 cm), flower weight (6.94 g) and flower diameter (7.07 cm) were the highest in the variety Phule Ganesh Pink. The variety Phule Ganesh White was superior in terms of number of disc florets per head (251.00) whereas Phule Ganesh Pink had the highest number of ray florets per head (201.20). The flower yield per plant was highest in variety Phule Ganesh Pink (55.99 g). The variety Phule Ganesh White recorded the highest seed yield per flower (1.27 g) and seed germination (65.27 %). Longest shelf life was observed in variety Phule Ganesh Pink (3.67 days) which was on par with Phule Ganesh White (3.33 days) and physiological loss of weight was recorded highest in Phule Ganesh White (35.70 %). The variety Phule Ganesh Pink had the longest vase life (13.93 days) and total water uptake was also maximum in variety Phule Ganesh Pink (20.27 ml). Anthocyanin content of flowers was recorded highest in the variety Phule Ganesh Violet (51.87 mg/g) followed by Arka Kamini (48.62 mg/g). Dendrogram based on D2 statistics considering qualitative and quantitative characters indicated that high amount of variability is present among the varieties. Correlation and path analysis revealed that the characters such as number of primary branches (0.978), plant spread (0.894), leaf area (0.796) and flower weight (0.933) were having significant positive correlation with flower yield per plant with a direct effect of 0.150, 0.199, 0.281 and 0.498 respectively. Ranking of varieties was done individually for loose flower and cut flower types. In ranking for loose flower types, variety Phule Ganesh Pink scored first position which was followed by Phule Ganesh White and Local Pink. When varieties were ranked for cut flower types, first position was scored by Phule Ganesh Pink while second position was shared by Phule Ganesh White, Phule Ganesh Purple and Local Pink. In overall ranking the variety Phule Ganesh Pink occupied first position followed by Phule Ganesh White and Local Pink and these can be recommended for commercial cultivation in tropical plains of Kerala during winter season.
Open Access
Standardization of growing media and growth regulators for rose (Rosa ssp.) under top ventilated rain shelter
(Department of Floriculture and Landscaping, College of Horticulture, Vellanikkara, 2019) Sijo, John; KAU; Mini, Sankar
Rose is acclaimed as ‘Queen of flowers’ because of its colour, fragrance and elegance. Cut roses are having very high demand in domestic as well as international market due to versatile uses. Roses can be successfully cultivated under top ventilated rain shelters in Kerala. Soilless media consist of vermiculite, perlite, and rockwool are popularly used for commercial production of rose under protected condition. These are costly and not easily available to farmers. Thus, there is a need to develop a cost effective growing media with locally available materials. Exogenous application of plant growth regulators improves the yield and quality of cut flowers by manipulating hormonal regulations in the plant system. Even though commercial formulations of different growth regulators are available in the market, the type and precise concentration of growth regulators are to be standardised. Hence the present study entitled “Standardization of growing media and growth regulators for rose (Rosa spp.) under top ventilated rain shelter” was carried out in the Department of Floriculture and Landscaping, College of Horticulture, Vellanikkara during 2018-2019. Objective of the study was to standardize growing media and growth regulators for commercial production of cut roses in agroclimatic conditions of Kerala. The experiment consisted of twelve treatments, which included different combinations of three growing media viz; soil + M-sand + cocopeat + FYM (M1), soil + M-sand + cocopeat + vermicompost (M2) and soil + M-sand + cocopeat + poultry manure (M3) in 2:1:1:1 ratio and two levels each of two growth regulators viz; Benzyl adenine (BA) and Gibberellic acid (GA) at 200 and 250 ppm. Six months old grafts of rose variety ‘Tajmahal’ was used for the study. Observations on growth characters, yield, post harvest aspects as well as nutrient status of the growing media were recorded. With regard to vegetative characters, growing media, growth regulators and interaction between growing media and growth regulators had significant influence during the period of observation. Growing media containing FYM (M1) recorded the greatest plant height and was on par with growing media containing vermicompost (M2). Among growth regulators, GA 250 ppm was found to be superior in terms of plant height. With respect to interaction effect, greatest plant height was observed under the media containing FYM (M1) and GA at 250 ppm. Plant spread was highest under medium containing FYM (M1). Growth regulators had significant influence on plant spread and BA at 200 and 250 ppm were superior and on par in terms of this parameter. Regarding interaction, growing media containing FYM (M1) along with 200 ppm BA was observed to have the highest plant spread during the growth period. Media containing FYM (M1) as well as vermicompost (M2) were found to produce more number of branches and among growth regulators, BA at 200 and 250 ppm were on par and superior with respect to this parameter. Regarding interaction effect, highest number of branches was observed under growing media containing FYM (M1) and BA at 200 ppm. Significant effects of growing media could be observed on floral characters and media containing FYM (M1) was found to be superior with respect to floral characters viz; length of flower bud (3.37 cm), diameter of flower bud (2.16 cm), number of flowers /plant (5.04), number of petals/ flower (51.92), stalk length (47.94 cm) and flower diameter at fully opened stage (8.44 cm). Growing media containing FYM (M1) and growing media containing vermicompost (M2) were on par in terms of other floral characters viz; length of flower shoot, neck girth and stalk girth of flower. With respect to the influence of growth regulators on floral characters, a greater number of flowers/plant (5.38 and 5.10) as well as largest flowers at fully opened stage (8.33 and 7.99 cm) were observed under BA at 200 and 250 ppm. Stalk length of the flower was found to be maximum under GA at 200 ppm (47.92 cm). Highest length of flower shoot was observed in GA at 200 ppm and BA at 200 ppm which were on par with each other (38.79 and 36.94 cm respectively). Growing media and growth regulator interaction had significant influence on floral parameters viz; stalk length and number of petals per flower. Media containing FYM (M1) with GA 200 as well as 250 ppm were found to have maximum stalk length (52.48 and 50.09 cm respectively). Highest number of petals were observed under media containing FYM (M1) along with BA 200 and 250 ppm, media containing FYM (M1) along with GA 200 and 250 ppm, media containing vermicompost (M2) along with BA 200 ppm and GA 200 ppm as well as media containing poultry manure (M3) along with BA 200 ppm. Early initiation of flower bud was observed under media containing FYM (M1) along with BA 250 ppm (119.22 days). Regarding post harvest characters, media containing FYM (M1) was superior in terms of fresh weight of flower (16.36 g), total water uptake (49.48 ml) and vase life (8.46 days). Among growth regulators BA 200 ppm had longest vase life which was on par with GA at 200 ppm. Maximum fresh weight was observed under BA 200 ppm (15.17g). With regard to interaction effect, treatment combination of FYM + BA at 200 was found to have maximum vase life and fresh weight of flower (9.38 days and 18.71g respectively). Considering overall performance, growing media combination of soil + M-sand + cocopeat + FYM (2:1:1:1) along with monthly application of BA at 200 ppm can be recommended for commercial cultivation of rose under protected condition in Kerala.
Open Access
Response of Papaya (Carica Papaya L.) to Major Mineral Nutrients
(Department of Pomology and Floriculture, College of Agriculture , Vellayani, 2003) Bindu, B; KAU; Jayachandran Nair, C S
An experiment was conducted in the Department of Pomology and Floriculture, College of Agriculture, Vellayani during 2001-2002, to study the response of major plant nutrients viz., nitrogen, phosphorus and potassium on growth, yield and quality of papaya under Kerala conditions and to standardise the optimum dose of these nutrients. The experiment was conducted in 33 confounded factorial RBD, confounding NPK in replication 1 and Np2K2 in replication 2. The present study revealed that application of nitrogen, phosphorus and potassium increased plant height, girth and number of leaves. The highest plant height was obtained with the combined application of 200 g N, 300 g P and 500 g K plan' year-', while the highest plant girth was obtained with application of 250 g N, 300 g P and 500 g K. Combined application of 300 g N, 250 g P and 500 g K p lant' year-' resulted in the maximum number of leaves. Plants receiving a dose of nitrogen at 250 g, 300 g phosphorus and 500 g potassium plant" took the shortest time for flowering. Combined application of nitrogen at 250 g plant.", phosphorus at 250 g plant" and potassium at 500 g planr' considerably shortened the time for harvesting the first fruit. Fruit weight, number of fruits plant", yield planr' and papain yield increased by application of nitrogen, phosphorus and potassium. Application of 250 g N, 250 g P and 500 g K planr' year-' gave highest yield. Maximum fruit girth was obtained from 200 g N, 200 g P and 500 g K, whereas maximum fruit volume and pulp percentage was obtained from 250 g N, 250 g P and 500 g K. Levels of nitrogen and phosphorus tried had no significant influence on TSS and ascorbic acid content of fruits. Nitrogen at 200 g, phosphorus at 250 g planr' produced. fruits of low acidity, while potassium had no significant influence. The combination of 200 g nitrogen, 300 g phosphorus and 500 g potassium plane 1 year" increased carotenoids, total sugars, reducing sugars and organoleptic qualities of fruits. Most of the treatments had light yellow peel colour, orange pulp colour and firm flesh. Nitrogen at 200 g, phosphorus at 250 g and potassium at 500 g plant" was found to increase the shelf life of fruits. Application of nitrogen, phosphorus and potassium increased soil and leaf petiole content of the respective elements. Highest benefit: cost ratio was obtained from the combination of 250 g N, 250 g P and 500 g K I -I -I P ant year . Over all assessment indicated that application of N, P and K at the rate of 250 : 250 : 500 g plant" year-I in six equal splits was economically viable and improved growth, yield and quality of papaya.
Open Access
Micropropagation and crop improvement of Cordyline (Cordyline terminalis (L.) Kunth)
(Department of pomology and floriculture, College of horticulture,Vellanikkara, 2000) Lavanya, R; KAU; Rajeevan, P K
Investigations on micropropagation and crop improvement of cordyline were carried out in the Department of Pomology and Floriculture, College of Horticulture, Vellanikkara during 1998-2000. The main objectives were to study the response of various explants and to identify the most suitable explant and media combination for in vitro propagation. Attempts were also made to find out the optimum dose of y - irradiation, for inducing variation. The shoot tips and nodal segments of cordyline were found to be ideal explants for the enhanced release of axillary buds and for indirect organogenesis. The best sterilization treatment was wiping with 70 per cent ethyl alcohol, followed by a dip in 0.1 per cent mercuric chloride for 18 min. for shoots, nodal segments and axillary buds. For leaf segments, treatment with 0.1 per cent mercuric chloride for ten minutes alone was enough. The nodal segment explants collected in the drier months, namely; January, March, November and December showed no contamination. Survival percentage was the highest in the months of November and December. The shoot tip explants showed least contamination when collected during August to April. Early release of buds (4.4 days after inoculation) and further growth of buds was better in MS medium supplemented with BAP 3.0 mg r'. Maximum shoot proliferation was observed in MS medium with 2.0 mg rI BAP. In the case of shoot tips MS medium having Kin 3.0 mg r' showed less time (5.5 days) for bud emergence. Maximum number of shoots (2.5) was also obtained when MS medium was supplemented with 3.0 mg rl KIN. MS medium supplemented with BAP in combination with NAA also proved better for culture establishment of nodal segments and shoot tips. Maximum number of shoots was observed in MS medium with NAA 0.5 mg r' + BAP 1.0 mg l". Of the different media tried, MS medium was found to be the best for early release of buds in nodal segments and shoot tips. Number of shoots produced did not differ significantly in all the three media. The elongated buds from Stage 1 showed very high rate of axillary bud production when inoculated in MS medium containing BAP 1.0 mg r' + NAA 0.5 mg r' and BAP 2.0 mg r' + NAA 0.5 mg r'. Among MS, SH and WPM media, full strength MS medium gave very high rate of axillary bud production within a short time (12.2 days). High rate of callus production was observed in MS medium having 1.0 mg r' BAP + 1.0 mg rl NAA and 2.0 mg rl BAP + 1.0 mg rl NAA. The rate of axillary bud production and callusing was less with KIN in combination with NAA. Irrespective of the media, rhizogenesis was observed when higher levels of 2ip was incorporated in to the media. Elongation of the multiple axillary buds with normal shoot and root growth was recorded in full strength MS medium devoid of growth regulators. None of the media supplements (activated charcoal, adenine sulphate, coconut water and casein hydrolysate) induced multiple axillary bud elongation. Among the three basal media tried, MS medium was superior with respect to the number of lengthy roots and the time taken for root initiation. Among the auxins, iliA was superior for root induction. Callus formation was observed at the base when NAA was incorporated in the media. Maximum percentage of rooting, early rooting and more number of lengthy roots were obtained in full strength MS basal medium. Addition of activated charcoal in the medium did not affect root number and root length, but reduced the number of days taken for root initiation. Triadimefon in the rooting medium slightly increased the number of days taken for root initiation and decreased the number of roots/culture and length of roots. It also increased the survival percentage during hardening. Maximum survival percentage of the plantlets was obtained when the plantlets rooted in the medium containing Triadimefon 1.0 mg r' + iliA 5.0 mg l' were treated with 0.1 per cent Bavistin for 30 minutes soon after removal from the culture vessels and subjected to a post planting treatment with triadimefon (20 mg rl) drenching at weekly intervals and kept in a net house having 50 per cent shade. Plant height was the maximum when the plantlets after planting out were supplied with 0.75 g 17: 17: 17 NPK mixture per week as soil drench. Among the various explants tried for somatic organogenesis, nodal segments and shoot tips were the most ideal for callus initiation, growth and differentiation. Callus intensity was maximum in the leaf segments when bottom portions of the immature leaves with midrib were cultured. Best response to callusing and callus intensity was obtained with the adaxial surface of the leaf touching the medium. Direct organogenesis could not be obtained in different treatment combinations or explants. Shoot tips and nodal segments produced higher percentage of callus and showed maximum callus index when MS medium was supplemented with lower levels of 2, 4 D (0.5 mg rl) and BAP (0.5 mg r'j, both in 12 h photoperiod. No positive response was observed with the addition of coconut water on callus induction and differentiation. Earliest differentiation of callus derived from nodal segment and shoot tip explants was possible in MS medium supplemented with BAP 1.0 mg r' + KIN 2.0 mg r': Very high rate of shoot production was observed in the medium supplemented with BAP 1.0 mg rl + KIN 2.0 mg r'. A combination of BAP + KIN gave high rate of shoot production, compared to BAP + 2ip combination. Various treatment combinations failed to induce morphogenesis in leaf derived callus, but additon of activated charcoal prevented browning of callus upon subculturing. Higher concentration of cytokinins prevented rhizogenesis. In vitro mutagenesis was attempted to know the optimum dose and correct stage for induction of variation. Of all the doses of y-irradiation, a dose of 10 Gy at culture establishment stage produced plants having narrow yellow leaves with green line at the centre, but after planting out the plantlets reverted to normal behaviour.
Open Access
Somatic Embryogenesis in Musa (AAB) Nendran
(Department of pomology and floriculture, College of horticulture,Vellanikkara, 2002) Beena, R; KAU; Aravindakshan, K
Open Access
Response of Ascocenda orchid to growth regulator and micronutrients
(Department of Floriculture and Landscaping, College of Horticulture, Vellanikkara, 2019) Jesabel George; KAU; Shobhana, A
A study entitled ‘Response of Ascocenda orchid to growth regulator and micronutrients’ was carried out at Department of Floriculture and Landscaping, College of Horticulture Vellanikkara, from May 2018 to May 2019. Ascocenda is a monopodial, epiphytic, bigeneric hybrid, which is mainly grown as pot plant in hanging baskets using bricks, charcoal, coconut husk pieces etc. as growing media. The objective of the study was to evaluate the influence of foliar application of different micronutrient treatments on growth and yield of Ascocenda orchid. The experiment was conducted with eleven treatments viz., 0.01% zinc + 150 ppm benzyl adenine + PoP (T1), 0.025% zinc + 150 ppm benzyl adenine + PoP (T2), 0.01% manganese + 150 ppm benzyl adenine + PoP (T3), 0.025% manganese + 150 ppm benzyl adenine + PoP (T4), 0.01% boron + 150 ppm benzyl adenine + PoP (T5), 0.025% boron + 150 ppm benzyl adenine + PoP (T6), 0.01% iron + 150 ppm benzyl adenine + PoP (T7), 0.025% iron + 150 ppm benzyl adenine + PoP (T8), 0.01% molybdenum + 150 ppm benzyl adenine + PoP (T9), 0.025% molybdenum + 150 ppm benzyl adenine + PoP (T10), 150 ppm benzyl adenine + PoP (T11 – control). Three month old tissue cultured plants of Ascocenda var. Big Suksamran were used for the study. The micronutrients were applied at fortnightly intervals and benzyl adenine was applied at monthly intervals. Application of NPK (3:1:1) weekly twice @ 0.2% and cow dung slurry (1:5) at monthly intervals was given to all treatments as per PoP recommendation of KAU. Observations were taken at monthly intervals. The results indicated that foliar application of 0.025% manganese along with 150 ppm BA and recommended dose of NPK (T4) was best for improving plant height. The maximum plant height obtained at 12MAP was 8.86 cm. This was followed by T5 (8.81 cm) and T3 (8.63 cm) which were statistically on par with T4. The maximum shoot diameter was observed in T5 (10.20 mm) at 12 MAP which was on par with T4 and T3 (9.96 mm and 9.84 mm respectively). The treatment T3 was superior in terms of leaf characters like leaf length and leaf area up to 7 MAP and thereafter these parameters were highest in treatment T5. However, there was no significant difference between T5 and T3 in terms of leaf length at 12 MAP (16.70 cm and 16.48 cm respectively). The highest leaf area at 12 MAP was observed in T5 (23.17 cm2) followed by T3 (22.73 cm2). Number of leaves and leaf breadth were found highest with the application of 0.01% boron along with 150 ppm BA and recommended dose of NPK. A maximum of 13.69 leaves were observed in T5 at 12MAP. The maximum leaf breadth observed in T5 after 12 months of planting was 1.52 cm, which was closely followed by T3 and T4 (1.51 cm each), and no significant difference between these three treatments could be noticed. Regarding interval of leaf production, only 4 treatments (T3, T4, T5, and T11) could produce the highest number of eight leaves, within a period of 386 days. Among these, T4 took the shortest period of 337.45 days to produce the 8th leaf. T10 produced only five leaves within a period of 386 days. Among the root parameters, highest root length was observed in T3 (0.01% manganese + POP + 150 ppm BA) at 12 MAP (26.59 cm) whereas the treatment T4 (0.025% Mn + PoP + 150 ppm BA) was superior in terms of number of roots and root diameter. The best treatment with respect to number of roots varied during initial months, even though, from 6 MAP onwards, highest number of roots was observed in T4 with a value of 10.28 at 12 MAP. In the case of root diameter, a highest of 2.91 mm was recorded in T4 at 12 MAP, which was on par with T8 (2.86 mm), T3 (2.84 mm) and T5 (2.82 mm). Among the eleven treatments, T3 (Mn 0.01% + PoP + 150ppm BA), T4 (Mn 0.025% + PoP + 150ppm BA), and T5 (B 0.01% + PoP + 150ppm BA)were found to be best for improving the vegetative characters of Ascocenda orchid, while application of Mo @ 0.025% (T10) at fortnightly intervals was inhibitory to the plants in terms of all the vegetative characters studied. The objective of studying the floral and postharvest characters could not be achieved since the plant did not bloom within the period of study.
Open Access
Evaluation of selected underutilized flowers of Kerala for commercial exploitation
(Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 2018) Sameera Sharief; KAU; Sudhadevi, P K
Floriculture industry is unique among agricultural industries where novelty is an important attribute. World floriculture is expanding rapidly and new innovations and introductions are in great demand to feed the ever hungry market needs. It is in this interest that neglected or underutilized flower crop species (NUS) comes to the picture from which we could identify and develop diversified uses of floriculture. Even the present day top charactered crops of the industry are nothing but just derived and developed only from wild germplasm resourses, the most prominent among them being rose, chrysanthemum, carnation, gerbera and what not, the orchids and anthurium. Thirteen underutilized plants of Kerala were evaluated for use as cut flowers, dry flower, for essential oil extraction and pigment extraction and identification of components in their essential oils and pigments using GC-MS by conducting both field studies as well as postharvest studies. Out of the 10 plants selected for studying their use as cut flower, none were found suitable. Five plants were selected for their suitability for dry flower production. Among them, Cassia fistula got the highest cumulative score followed byAntigonon leptopus,Calicopteris floribunda and Barleria obtusa. Least cumulative score was obtained for Clerodendrum paniculatum. In Antigonon leptopus and Clerodendrum paniculatum best method of drying was microwave oven drying. Press drying was selected as the best method for Barleria obtusa and Calicopteris floribunda. Embedded drying was found the most suitable method of drying in Cassia fistula. The fragrant flowers selected for extraction of essential oils were Gardenia jasminoides, Plumeria spp and Quisqualis indica.Maximum essential oil yield was observed in Gardenia jasminoides (0.61%). The components in the essential oils were identified by gas chromatography and mass spectrometry. In Gardenia jasminoides the components identified were Ascalbin (0.7%), Nonadecane (0.80%), Dendaralasine (0.96%), Alpha famesene (1.12%), Linalool (1.38%), Beta famesene (2.75%), Famesene (3.04%), Henecosane (5.26 %), n-Tricosane (6.91%), n-octacosane (10.43%), Pentacosane (13.19%), Monoethylhexyl phthalate (44.74 %). The volatile components identified in Plumeria were α-Farnesene (1.08%), Benzoic acid, [(E,E)-3,7,11-trimethyl- 2,6,10-dodecatrien-1-yl] ester (1.08%), 1,6,10-Dodecatriene, 7, 11- dimethyl- 3- methylene-E(1.17%), Cyclohexasiloxane, dodeca methyl (1.92%). Phenyl ethyl alcohol (2.20%), 1,3,6,10-Dodecatetraene, 3,7,11-trimethyl-(Z,E)(2.93%), Oxalic acid, decyl 2-phenyl ethyl ester (4.3%), Triphenyl phosphare (4.3%), Heptacosane (6.32%), Nonacosane(6.40%) and Z-14-Nonacosane (6.40%) and Z-14- Nonacosane(11.65%). The components responsible for fragrance in Quisqualis indica were 2 H- Pyran-3-ol, 6-ethenyl tetra hydro-2,2,6-trimethyl(1.24%), Heptacosane (1.44%), Cyclotetra siloxane, octamethyl (1.95), Triphenyl phosphate (2.30%), Nonacosane(2.89%) and 5-Isoquinoline carbonitr (12.5%). The flowers selected for extraction of pigments were Caesalpinia pulcherrima, Cassia fistula, Clerodendrum paniculatum and Delonix regia. Two methods of extraction selected were solvent extraction after fermentation and solvent extraction after pretreatment with NaOH. In the entire species pigment yield was higher for solvent extraction after pretreatment. Highest oleoresin yield was observed in Clerodendrum paniculatum (0.60g), which was followed by Delonix regia (0.5g) and Cassia fistula(0.39). The lowest yield was observed in Caesalpinia pulcherrima (0.38g). After fermentation, Clerodendrum paniculatum gave highest oleoresin yield (0.43g). This was followed by Delonix regia(0.4g), Cassia fistula(0.3g) and Caesalpinia pulcherrima(0.28g). Highest carotenoid yield was observed in Cassia fistula (70.04mg/ 100 g) and highest anthocyanin yield was in Clerodendrum paniculatum (574.76mg/100g)). In Caesalpinia pulcherima carotenoid content was recorded as 15.35mg/100g and anthocyanin 488.75mg/100g. Anthocyanin yield of Cassia fistula was 0.35mg. Clerodendrum paniculatum recorded 2.98mg of carotenoid. Delonix regia recorded carotenoid and anthocyanin yield of 60.2 mg and 510 mg respectively. In the present study none of flowers were found suitable for use as cut flower. Out of the 5 plants selected for studying for use as dry flower, Cassia fistulawas the most suitable one. All the species selected for essential oil extraction were suitable for the purpose .In pigment extraction, highest oleoresin yield was observed in Clerodendrum paniculatum (0.60g), which is followed by Delonix regia (0.5g) and Cassia fistula(0.39). Future line of work suggested in this aspect based on the light of results are evaluation of more underutilized ornamental flowers available in our locality with a view of their commercialisation for specific traits and further evaluation of extracted pigments for their use in food industry.
Open Access
Variability studies in rambutan (Nephelium lappaceum L.)
(Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 2016) Sameer Muhamed; KAU; Sajan Kurien
Rambutan ( Nephelium lappaceum L.) or ‘hairy litchi’ is an exotic fruit crop with tremendous potential for cultivation in the whole state of Kerala. There exists great variability in rambutan because of the natural cross pollination and seed propagation, which in turn provides great scope for selection. It is in this context that the hundred variable types of rambutan collected from important rambutan growing tracts of Kerala (Pathanamthitta-40, Kottayam-37, Thrissur-18 and Idukki-5) were evaluated for various morpho-physiochemical traits in addition to sensorial perception. The study was taken up with the prime objectives of recording the existing variability and genetic wealth available in order to select and conserve the elite/promising types and thereby, to serve as a basis for promotion of cultivation of this exotic fruit crop in Kerala. The study confirmed that rambutan fruits varied widely in their morpho-physical and biochemical traits such as tree characters, inflorescence characters, fruit characters and quality parameters, which were recorded as per IPGRI crop descriptor. Flowering and fruiting were characters more observed to be a function of the location. The study on phenological growth stages of rambutan according to BBCH scale in different locations opens out a new area on the possibility of staggered fruit production of rambutan in Kerala. The fruits are available from May to October (if cultivated in the humid tropical plains as in Thrissur, Kottayam and Pathanamthitta as well as in the humid sub-tropical belts as in the high ranges of Wayanad), thus making the availability of fresh fruits for nearly half period of a calendar year. With regard to the individual fruit weight, highest fruit weight of 61.5 g was recorded in Col.052 and the lowest fruit weight of 12 g was observed in Col.012 and Col.013. The largest proportionate edible part was found in Col.072 (52%) and the lowest in Col.008 (15%). Free seed aril was found in 19.39 per cent of the collections, while the adherence of aril to seed was medium in 16.33 per cent of the collections. Only 8.16 per cent of the collections produced fruits which could be characterized as very juicy types and 47.99 per cent were grouped as juicy type. About half of the collections (48.97%) had a TSS above 20° Brix. The collections having TSS above 25° Brix (Col.015 with 26° Brix and Col.061 with 27.5° Brix) were classified under the category ‘very sweet’. The titrable acidity and total sugar content ranged from 0.12 to 1.4% and 13 to 18.4% respectively. The loading plot based on tree characters revealed significant positive correlations between rachis length and leaflet width; leaflet width and leaflet length and between tree age and number of leaflets per leaf. Strong positive associations were also observed among fruit characters such as fruit weight, fruit diameter, rind weight, aril weight, aril thickness and spine length. The principal component analysis performed based on tree characters revealed the major contribution of leaflet length, leaflet width and petiole length towards the genetic divergence in rambutan and about 75 per cent of the total variance in fruit characters was mainly determined by the traits such as fruit weight, aril weight, aril thickness, seed weight, seed width, titrable acidity, TSS and sugar content. Clustering of all the three categories of rambutan collections namely elite, export and industrial use types using score plot confirmed the superiority of six collections (Col.021, Col.022, Col.023, Col.052, Col.55 and Col.061) over others, which were selected further for developing a selection criteria in rambutan. In the sensory analysis, Col.061, Col.021 and Col.042 were most preferred/accepted by panelists because of their better fruit weight, taste, juiciness and easy detachment of aril from seed. Two new pests of economic importance (fruit borer, Conogethes punctiferalis and fruit webber, Eublemma anguilifera) have been identified from the rambutan growing tracts of Kerala from fruit set to fruit ripening stage. Their nature of damage and symptoms of infestation have been clearly studied and being reported for the first time in rambutan from India. The study revealed that rambutan selections from Kerala are not only comparable with the best known cultivars in the world, but even excell them. There is an urgent need to standardize the management practices including the varietal wealth in rambutan by using these genetic resources as this crop has already become one of the most treasured fruit particularly as a courtyard crop in Kerala.
Open Access
Nutrient management in gerbera (Gerbera jamesonii Bolus.) grown under naturally ventilated polyhouse
(Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2018) Anaswara, S J; KAU; Sheela, V L
The thesis work entitled “Nutrient management in gerbera (Gerbera jamesonii Bolus.) grown under naturally ventilated poly house” was carried out at the Department of Pomology and Floriculture, College of Agriculture, Vellayani during 2016-2018. The study was to assess the performance of five gerbera varieties to different doses of fertilizers with a view to find out the best fertilizer dose for each variety. This study also aimed for the standardization of nutrient management schedule for Gerbera jamesoniiBolus. Five varieties were selected for the study (V1- Beaudine, V2- Double date, V3- Mammut, V4- Esmara, V5- Aquamelon). Different nutrient treatments (N1- 10:10:10 N, P2O5, K2O g m-2, N2- 15:15:15 N, P2O5, K2O g m-2, N3- 20:20:20 N, P2O5, K2O g m-2) were applied to the varieties at monthly and fortnightly interval. The experiment was laid out in completely randomised design consisting of thirty treatment combinations and three replications. The study revealed that variety Beaudine was significantly superior in terms of vegetative parameters viz., plant spread (156.19cm) at 4 MAP, number of leaves (13.31) at 8 MAP and leaf length (37.09cm) at 4MAP. Variety Mammut was significantly superior in leaf breadth (11.03cm) at 10 MAP and number of suckers plantˉ1(2.59) at 6 MAP. The nutrient treatment 10:10:10 N, P2O5, K2O g mˉ2 produced significantly higher plant spread (171.29cm) at 10 MAP, number of leaves (12.54) at 6 MAP, leaf length (34.04cm) at 4 MAP, leaf breadth (12.35cm) at 4 MAP and number of suckers plant-1 (2.97) at 6 MAP. The level of split application of nutrients s1 showed significantly superior values for plant spread (148.86cm) at 4 MAP, number of leaves (10.95) at 8MAP, leaf length (31.67cm) at 4 MAP, leaf breadth (10.79cm) at 4MAP and number of suckers plantˉ1 (2.42) at 6MAP. The variety Esmara when treated with 10:10:10 N, P2O5, K2O g mˉ2 at monthly interval of split application of nutrients recorded significantly highest value for plant spread (183.38cm) at 10 MAP. Variety Beaudine treated with 10:10:10 N, P2O5, K2O g mˉ2 (1.6: 1.6: 1.6 N, P2O5, K2O g plantˉ1)at monthly interval of split application of nutrients recorded significantly higher values for number of leaves (15.23) at 6MAP and leaf length (46cm) at 4 MAP. Variety Mammut treated with 10:10:10 N, P2O5, K2O g mˉ2 split application of nutrients at monthly intervals recorded significantly higher values for leaf breadth (15.74cm) at 4 MAP and number of suckers plantˉ1 (3.89) at 6 MAP. Regarding the flowering characters, Variety Esmara treated with 10:10:10 N, P2O5, K2O g mˉ2 of split application of nutrients at monthly intervals recorded the lowest value for number of days taken for flowering (17) and variety Double date treated with 15:15:15 N, P2O5, K2O g mˉ2 at fortnightly interval of split application of nutrients recorded lowest value for number of days taken from bud initiation to harvest (7.9) and there was no significant difference on flower longevity on the plant. In flower characters, Mammut variety supplied with a combination of 10:10:10 N, P2O5, K2O g mˉ2 fertilizers at monthly interval of split application of nutrients recorded highest flower diameter (11.24cm) and width of ray florets (1.00cm).There was no significant difference observed for flower disc diameter and length of the ray florets. Esmara variety with a combination of 10:10:10 N, P2O5, K2O g mˉ2 fertilizers at monthly interval of split application of nutrients recorded the highest number of ray florets (65.01).Beaudine variety with a combination of 10:10:10 N, P2O5, K2O g mˉ2 fertilizers at monthly interval of split application of nutrients recorded the highest stalk girth (2.39cm) and stalk length (79.34cm). In yield parameters, highest number of flowers produced plant-1year-1(25.75) and higher yield of flowers in relation to season (15.25) at rainy season was recorded for variety Esmara treated with a combination of 10:10:10 N, P2O5, and K2O g mˉ2 with a split application of nutrients at monthly intervals. The vase life of flowers and water uptake showed no significant difference among the treatment combinations All the five varieties while treated with 10:10:10 N, P2O5, K2O g mˉ2 at monthly intervals resulted high values for vegetative, flower and flowering characters. The best performance for Beaudine followed by Esmara and Aquamelon. BC ratio was highest for Beaudine. Future line of work may be standardisation of agrotechniques for gerbera under Kerala conditions.

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