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2018 - 20193
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Start date: 2010 × End date: 2019 × Subject: Veterinary Anatomy ×
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    ThesisItemOpen Access
    GROSS MORPHOLOGICAL, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON PINEAL, PITUITARY, THYROID AND ADRENAL GLANDS OF JAFFARABADI BUFFALO DURING DIFFERENT SEASONS OF THE YEAR 3013
    (JAU, JUNAGADH, 2019-10) ANIL SHARMA; Y. L. VYAS
    The present study was conducted to evaluate the effect of seasons on gross morphological, histomorphological and histochemical changes in the pineal, pituitary, thyroid, and adrenal glands of Jaffarabadi buffaloes. The pineal, pituitary, thyroid and adrenal glands of 30 Jaffarabadi buffalo were collected from abattoir, during winter, summer and rainy seasons (10 samples during each season). The gross morphometric observation revealed that the pineal glands were heavier during the summer whereas the pituitary, thyroid and adrenal glands were heavier during the rainy season. The histomorphometric observations revealed that in the pineal gland, the nuclear diameter of pinealocyte, pinealocyte density, and number of Argyrophilic Nucleolar Organizer Region (AgNOR) was highest during the winter season. The brain sands were observed in all the pineal glands but occupied a larger area of pineal parenchyma during the summer as compared to winter season. In the pars distalis of the pituitary, larger diameter and a higher percentage of basophils were observed during winter and rainy seasons while the larger diameters and a higher percentage of acidophils were observed during the summer season. In pars tuberalis and pars intermedia, the nuclear diameter and cellular density were higher during summer. The thyroid glands in winter season were predominated by a higher number of small follicles lined by high cuboidal epithelium. However, the thyroid glands in summer and rainy seasons were predominated by a comparatively higher number of large follicles lined by flat epithelium. In the adrenal glands, thicker zona glomerulosa and medulla, with larger cells and nuclei were observed during summer, whereas the thicker zona fasciculata and zona reticularis with larger cells were observed during rainy and winter seasons. The present observations indicated that the pineal and thyroid glands of Jaffarabadi buffaloes were morphologically more active during winter season. In pituitary gland, the basophils showed higher activity during winter and rainy seasons, while the acidophils were more active during summer. The cells of pars tuberalis and pars intermedia showed higher activity during the summer season. In adrenal glands, the zona glomerulosa and medulla showed increased cellular activity during summer, whereas the cells of zona fasciculata and zona reticularis were more active during rainy and winter seasons. The present study clearly depicted the influence of seasons on cellular architecture of endocrine glands which is essential for physiological adjustment of Jaffarabadi buffalo to adapt in different environmental conditions.
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    ThesisItemOpen Access
    GROSS MORPHOLOGICAL, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON PINEAL, PITUITARY, THYROID AND ADRENAL GLANDS OF JAFFARABADI BUFFALO DURING DIFFERENT SEASONS OF THE YEAR 3013
    (JAU, JUNAGADH, 2019-10) ANIL SHARMA; Dr. Y. L. VYAS
    The present study was conducted to evaluate the effect of seasons on gross morphological, histomorphological and histochemical changes in the pineal, pituitary, thyroid, and adrenal glands of Jaffarabadi buffaloes. The pineal, pituitary, thyroid and adrenal glands of 30 Jaffarabadi buffalo were collected from abattoir, during winter, summer and rainy seasons (10 samples during each season). The gross morphometric observation revealed that the pineal glands were heavier during the summer whereas the pituitary, thyroid and adrenal glands were heavier during the rainy season. The histomorphometric observations revealed that in the pineal gland, the nuclear diameter of pinealocyte, pinealocyte density, and number of Argyrophilic Nucleolar Organizer Region (AgNOR) was highest during the winter season. The brain sands were observed in all the pineal glands but occupied a larger area of pineal parenchyma during the summer as compared to winter season. In the pars distalis of the pituitary, larger diameter and a higher percentage of basophils were observed during winter and rainy seasons while the larger diameters and a higher percentage of acidophils were observed during the summer season. In pars tuberalis and pars intermedia, the nuclear diameter and cellular density were higher during summer. The thyroid glands in winter season were predominated by a higher number of small follicles lined by high cuboidal epithelium. However, the thyroid glands in summer and rainy seasons were predominated by a comparatively higher number of large follicles lined by flat epithelium. In the adrenal glands, thicker zona glomerulosa and medulla, with larger cells and nuclei were observed during summer, whereas the thicker zona fasciculata and zona reticularis with larger cells were observed during rainy and winter seasons. The present observations indicated that the pineal and thyroid glands of Jaffarabadi buffaloes were morphologically more active during winter season. In pituitary gland, the basophils showed higher activity during winter and rainy seasons, while the acidophils were more active during summer. The cells of pars tuberalis and pars intermedia showed higher activity during the summer season. In adrenal glands, the zona glomerulosa and medulla showed increased cellular activity during summer, whereas the cells of zona fasciculata and zona reticularis were more active during rainy and winter seasons. The present study clearly depicted the influence of seasons on cellular architecture of endocrine glands which is essential for physiological adjustment of Jaffarabadi buffalo to adapt in different environmental conditions
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    ThesisItemOpen Access
    HISTOLOGICAL, HISTOCHEMICAL AND SCANNING ELECTRON MICROSCOPIC STUDIES ON THE OVIDUCT AND UTERUS OF JAFFARABADI BUFFALO (Bubalus bubalis) DURING FOLLICULAR AND LUTEAL PHASES 2514
    (JAU,JUNAGADH, 2018-04) VISHNUDEO KUMAR; Dr. K. N. VYAS
    The present study was conducted to characterize histomorphological, histochemical and scanning electron microscopic changes during follicular and luteal phases in oviduct and uterus of twenty adult Jaffarabadi buffaloes. Oviductal mucosa showed extensive anastomosing folds. The folds increased in number and height during follicular phase and decreased from infundibulum towards isthmus. Epithelium was pseudostratified columnar having ciliated and secretory cells. Height of epithelium was more in follicular phase. The thickness of tunica muscularis in isthmus increased significantly which has important role in sperm transportation. Endometrial epithelium was of pseudostratified columnar and glands were of simple coiled, branched tubular type. The height of lining and glandular epithelium were more in follicular phase. Glands were more coiled and branched during luteal phase. Cervical mucosa was thrown into longitudinal folds. Uterine glands were absent in cervical region. Surface epithelium was simple columnar type including goblet cells, and few ciliated cells. In follicular phase, oviductal epithelium showed strong reaction for neutral mucopolysaccharides whereas, stroma of the mucosal folds and tunica muscularis showed moderate reaction while tunica serosa showed weak to moderate reactions. Weak to moderate lipid reaction was observed in the apical part of oviductal epithelium. Uterine epithelium showed moderate reaction for neutral whereas, weak for acid mucopolysaccharides. Tunica muscularis was moderately positive for neutral and acidic mucopolysaccharides. In follicular phase, cervical epithelium showed intense AB-PAS reaction while moderate reaction observed during luteal phase. Cervical glands showed intense positive reaction for acid mucopolysaccharide. Scanning electron microscopic observations, revealed large number of longitudinal folds in oviductal mucosa lined by ciliated and nonciliated cells. In follicular phase, epithelium was densely ciliated with few non ciliated cells. In luteal phase, epithelial lining of infundibulum and ampulla had more number of secretory cells as compared to follicular phase. Isthmus showed lesser differentiation in ciliation between oestrous phases. Uterine epithelium consisted of ciliated and nonciliated cells. Ciliated cells were more in follicular phase whereas, secretory cell were more during luteal phase. Narrow cervical folds were separated by small crypts, lined by ciliated and nonciliated epithelial cells. In this study histomorphological, histochemical and scanning electron microscopic changes during follicular and luteal phases in oviduct and uterus of Jaffarabadi buffaloes were established