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Start date: 2010 × End date: 2019 × Subject: Aquaculture ×
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    “EFFECT OF SEAWEED Caulerpa spp. AS DIETARY INGREDIENT ON GROWTH PERFORMANCE AND SURVIVAL OF Labeo rohita (Hamilton, 1822) FRY” 3019
    (JAU, JUNAGADH, 2019-10) SOLANKI RAVINA DHIRUBHAI; N. H. JOSHI
    The study was conducted to evaluate the efficacy of seaweed (Caulerpa spp.) as feed additive on specific growth rate, weight gain, survival and some biochemical parameters in rohu (Labeo rohita) fry for 60 days. The five experimental diets were prepared by adding Caulerpa spp. at 0% (control diet), 3% (T1), 6% (T2), 9% (T3) and 12% (T3) inclusion level. Total 150 numbers of rohu (Labeo rohita) fry were randomly distributed into five treatments (control + treatment), each in three replicate. Sampling was carried out at every 15 days to monitor growth performance and to adjust rations. The water quality parameters viz. DO, alkalinity, pH and temperature was found in the permissible range during entire experimental period. Based on the results, there was a significant difference (P>0.05) reported in weight and length gain of fry fed with the different feeds during a period of 60 days. Fishes fed with T2 (Treatment) diet showed the highest mean weight gain, specific growth rate (SGR) and protein efficiency ratio (PER) than those fed with other diets. Lowest feed conversion ratio (FCR) and highest specific growth rate (SGR) SGR was obtained in T2.In the proximate analyses of the fish carcass the protein content was significantly highest in T2 compared to other treatments. The fat content was significantly higher in T4. From the findings of the current study it can be concluded that seaweed Caulerpa spp. is a potential protein rich basal ingredient and can be used to as good feed additive if used 6% level in the diets of rohu (Labeo rohita) fry without compromising its growth performance.
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    ThesisItemOpen Access
    “EFFECT OF PARTIAL REPLACEMENT OF FISH MEAL WITH EARTHWORM (Eisenia fetida) MEAL IN THE DIET OF Labeo rohita FRY” 3018
    (JAU, JUNAGADH, 2019-09) SOSA KANAK JETHABHAI; K. H. Vadher
    The experiment on “Effect of partial replacement of fish meal with earthworm meal in the diet of Labeo rohita fry” was conducted during the September to November, 2018 in the wet laboratory of College of Fisheries Science, Junagadh Agricultural University, Veraval. The objective of the present study was to find out the effect of partial replacement of fish meal with earthworm meal in the diet of L. rohita fry. In this experiment, the data pertaining to growth, survival rate, feed conversion ratio and protein efficiency ratio of L. rohita fry at partial replacement of earthworm meal were evaluated. L. rohita fry (average weight 0.103gm) were stocked at a density of 10 nos. /aquarium in all 20 aquaria. Five experimental diets were formulated and prepared. Approximately 35% protein level was maintained in all the diets. Diet prepared without earthworm meal protein (control) and other four diets containing 5%, 10%, 15% and 20% earthworm meal protein were prepared and fed to L. rohita fry at 10% body weight two times in a day for 60 days. Highest mean weight gain, SGR, PER and lowest FCR were recorded in 10% (T3) earthworm meal protein diet. 100% Survival rate were recorded in treatment including control diet. The result of the present investigation revealed that partial replacement of earthworm meal significantly affected the mean weight gain, SGR, survival rate, FCR and PER of L. rohita fry.
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    ThesisItemOpen Access
    ADOPTION OF IMPROVED SHRIMP FARMING PRACTICES IN SAURASHTRA REGION OF GUJARAT 3002
    (JAU, JUNAGADH, 2019-07) TANK KETAN VALLABHDAS; S. I. Yusufza
    Litopenaeus vannamei farming is playing a pivotal role in the socio-economic development of coastal districts of India. India is exporting 4.4 lakh tonnes of shrimp products (2016-17) of which L. vannamei share is 3.30 lakh tonnes. The present study was carried out in all coastal districts of Saurashtra viz. Bhavnagar, Amreli, Gir-Somnath, Junagadh, Porbandar, Devbhumi Dwarka and Jamnagar and Union Territory of Diu. Compendium of act, rules, guidelines, regulations and other notifications (2014) by Coastal Aquaculture Authority of India, Chennai was used as a base for compilation of interview schedule. Descriptive statistical tools was employed wherever required. The work was done with five objectives viz. adoption, knowledge, constraints, GIS imagery and socio-economic profile of the shrimp farmer. Adoption of shrimp farming practices indicated that 54.88% respondents were found to be partial adopters. Major adoption by the farmers viz. jaggery, yeast, vegetable meals and rice was used for water enrichment (100%), SPF healthy seed (100%), DO (100%), nutritionally balance feed (100%), probiotics (99.53%) and HDPE liner (51.63%). If disease outbreak occurs then 2.79% of farmers knows about treatment of the disease. Farmers having enough knowledge about HDPE liner (78.14%), nutritionally balanced supplementary feed (100%), nutritional enrichment (100%), probiotics (99.53%), chain dragging and sludge management (44.19%). Major constraints farmers faced were lack of disease diagnose lab (97.21%), non-availability of land (89.30%), price fluctuation during harvest (88.84%), lack of experience (86.51%), high initial investment (82.33%) and lack of proper extension network (60.47%). Detailed mapping of Saurashtra shrimp farms using high resolution Google imagery was carried out using QGIS software. Socio-economic data revealed that 94.42% farmers having their private land, 75.35% farmers have education above secondary, 58.60% farmers had more than five members in family, 58.44% respondents were having farming experience upto five years and 51.63% partnership type firm. Education and experience were positive and highly significant correlated to the adoption of shrimp farming. The result of the present study was very promising that all the farmers were high adoption rate and follow the guidelines of Coastal Aquaculture Authority at great extent. They have also utilized this knowledge for better profit gaining.
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    ThesisItemOpen Access
    EVALUATION OF SELECTED MICROBIAL SYMBIONTS FROM GASTROINTESTINAL TRACT OF GIFT TILAPIA (Oreochromis niloticus) AS POTENTIAL PROBIOTICS IN Litopenaeus vannamei (BOONE, 1931) 3021
    (JAU, JUNAGADH, 2019-11) ANITA; S. I. YUSUFZAI
    Present study was designed for evaluation of selected microbial symbionts isolated from gastrointestinal tract of GIFT tilapia (Oreochromis niloticus) as potential probiotics in Litopenaeus vannamei (Boone, 1931). Total 40 colonies were isolated from the gastrointestinal tract and on the basis of gram’s staining 15 colonies were discarded on the basis of colony morphology study and 25 colonies were purified by quadrant streaking method. Out of 25 different colonies 10 best performing colonies were selected for further study. All the colonies were screened for extracellular enzyme production. The zone of hydrolysis was measured and enzymatic activity index was calculated for different colonies. Colony C5 showed highest enzymatic index for amylase production (7.5) while colonies C9 and C10 were negative to amylase. Colony C4 showed highest enzymatic index for cellulase production (5) while colonies C9 and C10 were negative to cellulase. For chitinase colony C2, C6 and C8 showed highest enzymatic index for chitinase production (6) while, colonies C9 and C10 were negative to chitinase. Colony C4 and C9 showed highest enzymatic index for lipase production (7) while colonies C1 and C8 showed lowest enzymatic activity index (5) to lipase. For protease Colony C9 showed highest enzymatic index for amylase production (8) while colonies C2 showed lowest (3.6). The quantitative analysis of protease showed highest protease production by C9 (21.49 µg/ml) while, colony C4 produced (17.698 µg/ml) protease. Molecular identification of colonies showed that C1 and C3 were found to be homologous with Bacillus sonorensis, C2, C5, C6 and C7 were found to be homologous with Pseudomonas, C4 was found to be homologous with Bacillus thuringiensis, C8 was found to be homologous with Pseudomonas fragi while C9 and C10 were found to be homologous with Lactobacillus fermentum. Colony C1 Bacillus sonorensis was ii selected for probiotic in diet of L. vannamei. The culture of B. sonorensis was lyophilized and added in the shrimp diet @ 0.5gm in 500gm of feed. The feeding trial was conducted for 60 days. The effect of B. sonorensis was evaluated on SGR, FCR, PER and GCE. Four treatment groups T1(100% fish meal), T2 (50% fish meal and 50% soybean meal), T3 (25% fish meal and 75% soybean meal supplement) and T4 (100% soybean meal) were without probiotic supplementation while treatments T5 (100% fish meal with probiotic supplement), T6 (50% fish meal and 50% soybean meal with probiotic supplement), T7 (25% fish meal and 75% soybean meal with probiotic supplement) and T8 100% (soybean meal with probiotic supplement). The highest final wet weight gain was observed in T6 (3.62±0.08) and lowest in T4 (2.10±0.06g) treatments (p>0.05).The highest net weight gain was found in treatment T6 (3.42gm), where as the lowest was found in T4 diet (1.9gm). The highest net length was observed in T6 (8.4±0.06 cm) and lowest in T3 (6.93±0.03 cm) treatments (p>0.05). Data for average net length gain of post larvae of L. vannamei fed without probiotic and with probiotic diets showed highest net length in treatment T6 (6.6 cm), whereas the lowest was found in T4 diet (5.17 cm). Highest SGR was found in T6 diet treatment followed by T2, T3, T7, T8, T5, T1 and lowest in T8. Highest FCR was found in T4 diet treatment followed by T1, T5, T3, T8, T7, T2 and lowest in T6. Thus, the lowest FCR was observed in T6 (50% FM and 50% SM with probiotic supplement). No significant difference (p>0.05) was FCR values observed among the treatment diets. Highest PER was found in T6 diet treatment followed by T2, T7, T8, T3, T5, T1 and lowest in T4. Highest GCE was found in T6 diet treatment followed by T2, T3, T8, T7, T5, T1 and lowest in T4. The findings of the above study indicates that the treatment T6 showed higher growth of post larvae of L. vannamei at the end of the experiment along with higher value of SGR (2.15 ± 0.19), FCR (0.35 ± 0.01), PER (7.13±0.11) and GCE (2.85 ± 0.01) for treatment T6 (50% of fish meal and 50% soybean meal with probiotic supplementation). From the findings of the above study it can be concluded that the inclusion of B. sonorensis probiotic strain isolated from gastrointestinal tract of GIFT tilapia in a shrimp feed can help in 50% of fish meal replacement by soybean meal in diets of shrimps without compromising with growth and survival of L. vannamei.
  • Thumbnail Image
    ThesisItemOpen Access
    ADOPTION OF IMPROVED SHRIMP FARMING PRACTICES IN SAURASHTRA REGION OF GUJARAT 3002
    (JAU, JUNAGADH, 2019-07) TANK KETAN VALLABHDAS; S. I. Yusufzai
    Litopenaeus vannamei farming is playing a pivotal role in the socio-economic development of coastal districts of India. India is exporting 4.4 lakh tonnes of shrimp products (2016-17) of which L. vannamei share is 3.30 lakh tonnes. The present study was carried out in all coastal districts of Saurashtra viz. Bhavnagar, Amreli, Gir-Somnath, Junagadh, Porbandar, Devbhumi Dwarka and Jamnagar and Union Territory of Diu. Compendium of act, rules, guidelines, regulations and other notifications (2014) by Coastal Aquaculture Authority of India, Chennai was used as a base for compilation of interview schedule. Descriptive statistical tools was employed wherever required. The work was done with five objectives viz. adoption, knowledge, constraints, GIS imagery and socio-economic profile of the shrimp farmer. Adoption of shrimp farming practices indicated that 54.88% respondents were found to be partial adopters. Major adoption by the farmers viz. jaggery, yeast, vegetable meals and rice was used for water enrichment (100%), SPF healthy seed (100%), DO (100%), nutritionally balance feed (100%), probiotics (99.53%) and HDPE liner (51.63%). If disease outbreak occurs then 2.79% of farmers knows about treatment of the disease. Farmers having enough knowledge about HDPE liner (78.14%), nutritionally balanced supplementary feed (100%), nutritional enrichment (100%), probiotics (99.53%), chain dragging and sludge management (44.19%). Major constraints farmers faced were lack of disease diagnose lab (97.21%), non-availability of land (89.30%), price fluctuation during harvest (88.84%), lack of experience (86.51%), high initial investment (82.33%) and lack of proper extension network (60.47%). Detailed mapping of Saurashtra shrimp farms using high resolution Google imagery was carried out using QGIS software. Socio-economic data revealed that 94.42% farmers having their private land, 75.35% farmers have education above secondary, 58.60% farmers had more than five members in family, 58.44% respondents were having farming experience upto five years and 51.63% partnership type firm. Education and experience were positive and highly significant correlated to the adoption of shrimp farming. The result of the present study was very promising that all the farmers were high adoption rate and follow the guidelines of Coastal Aquaculture Authority at great extent. They have also utilized this knowledge for better profit gaining.
  • Thumbnail Image
    ThesisItemOpen Access
    EVALUATION OF SELECTED MICROBIAL SYMBIONTS FROM GASTROINTESTINAL TRACT OF GIFT TILAPIA (Oreochromis niloticus) AS POTENTIAL PROBIOTICS IN Litopenaeus vannamei (BOONE, 1931)3021
    (JAU, JUNAGADH, 2019-11) ANITA; S. I. YUSUFZAI
    Present study was designed for evaluation of selected microbial symbionts isolated from gastrointestinal tract of GIFT tilapia (Oreochromis niloticus) as potential probiotics in Litopenaeus vannamei (Boone, 1931). Total 40 colonies were isolated from the gastrointestinal tract and on the basis of gram’s staining 15 colonies were discarded on the basis of colony morphology study and 25 colonies were purified by quadrant streaking method. Out of 25 different colonies 10 best performing colonies were selected for further study. All the colonies were screened for extracellular enzyme production. The zone of hydrolysis was measured and enzymatic activity index was calculated for different colonies. Colony C5 showed highest enzymatic index for amylase production (7.5) while colonies C9 and C10 were negative to amylase. Colony C4 showed highest enzymatic index for cellulase production (5) while colonies C9 and C10 were negative to cellulase. For chitinase colony C2, C6 and C8 showed highest enzymatic index for chitinase production (6) while, colonies C9 and C10 were negative to chitinase. Colony C4 and C9 showed highest enzymatic index for lipase production (7) while colonies C1 and C8 showed lowest enzymatic activity index (5) to lipase. For protease Colony C9 showed highest enzymatic index for amylase production (8) while colonies C2 showed lowest (3.6). The quantitative analysis of protease showed highest protease production by C9 (21.49 µg/ml) while, colony C4 produced (17.698 µg/ml) protease. Molecular identification of colonies showed that C1 and C3 were found to be homologous with Bacillus sonorensis, C2, C5, C6 and C7 were found to be homologous with Pseudomonas, C4 was found to be homologous with Bacillus thuringiensis, C8 was found to be homologous with Pseudomonas fragi while C9 and C10 were found to be homologous with Lactobacillus fermentum. Colony C1 Bacillus sonorensis was ii selected for probiotic in diet of L. vannamei. The culture of B. sonorensis was lyophilized and added in the shrimp diet @ 0.5gm in 500gm of feed. The feeding trial was conducted for 60 days. The effect of B. sonorensis was evaluated on SGR, FCR, PER and GCE. Four treatment groups T1(100% fish meal), T2 (50% fish meal and 50% soybean meal), T3 (25% fish meal and 75% soybean meal supplement) and T4 (100% soybean meal) were without probiotic supplementation while treatments T5 (100% fish meal with probiotic supplement), T6 (50% fish meal and 50% soybean meal with probiotic supplement), T7 (25% fish meal and 75% soybean meal with probiotic supplement) and T8 100% (soybean meal with probiotic supplement). The highest final wet weight gain was observed in T6 (3.62±0.08) and lowest in T4 (2.10±0.06g) treatments (p>0.05).The highest net weight gain was found in treatment T6 (3.42gm), where as the lowest was found in T4 diet (1.9gm). The highest net length was observed in T6 (8.4±0.06 cm) and lowest in T3 (6.93±0.03 cm) treatments (p>0.05). Data for average net length gain of post larvae of L. vannamei fed without probiotic and with probiotic diets showed highest net length in treatment T6 (6.6 cm), whereas the lowest was found in T4 diet (5.17 cm). Highest SGR was found in T6 diet treatment followed by T2, T3, T7, T8, T5, T1 and lowest in T8. Highest FCR was found in T4 diet treatment followed by T1, T5, T3, T8, T7, T2 and lowest in T6. Thus, the lowest FCR was observed in T6 (50% FM and 50% SM with probiotic supplement). No significant difference (p>0.05) was FCR values observed among the treatment diets. Highest PER was found in T6 diet treatment followed by T2, T7, T8, T3, T5, T1 and lowest in T4. Highest GCE was found in T6 diet treatment followed by T2, T3, T8, T7, T5, T1 and lowest in T4. The findings of the above study indicates that the treatment T6 showed higher growth of post larvae of L. vannamei at the end of the experiment along with higher value of SGR (2.15 ± 0.19), FCR (0.35 ± 0.01), PER (7.13±0.11) and GCE (2.85 ± 0.01) for treatment T6 (50% of fish meal and 50% soybean meal with probiotic supplementation). From the findings of the above study it can be concluded that the inclusion of B. sonorensis probiotic strain isolated from gastrointestinal tract of GIFT tilapia in a shrimp feed can help in 50% of fish meal replacement by soybean meal in diets of shrimps without compromising with growth and survival of L. vannamei
  • Thumbnail Image
    ThesisItemOpen Access
    “EFFECT OF SEAWEED Caulerpa spp. AS DIETARY INGREDIENT ON GROWTH PERFORMANCE AND SURVIVAL OF Labeo rohita (Hamilton, 1822) FRY” 3019
    (JAU, JUNAGADH, 2019-10) SOLANKI RAVINA D.; DR. N. H. JOSHI
    The study was conducted to evaluate the efficacy of seaweed (Caulerpa spp.) as feed additive on specific growth rate, weight gain, survival and some biochemical parameters in rohu (Labeo rohita) fry for 60 days. The five experimental diets were prepared by adding Caulerpa spp. at 0% (control diet), 3% (T1), 6% (T2), 9% (T3) and 12% (T3) inclusion level. Total 150 numbers of rohu (Labeo rohita) fry were randomly distributed into five treatments (control + treatment), each in three replicate. Sampling was carried out at every 15 days to monitor growth performance and to adjust rations. The water quality parameters viz. DO, alkalinity, pH and temperature was found in the permissible range during entire experimental period. Based on the results, there was a significant difference (P>0.05) reported in weight and length gain of fry fed with the different feeds during a period of 60 days. Fishes fed with T2 (Treatment) diet showed the highest mean weight gain, specific growth rate (SGR) and protein efficiency ratio (PER) than those fed with other diets. Lowest feed conversion ratio (FCR) and highest specific growth rate (SGR) SGR was obtained in T2.In the proximate analyses of the fish carcass the protein content was significantly highest in T2 compared to other treatments. The fat content was significantly higher in T4. From the findings of the current study it can be concluded that seaweed Caulerpa spp. is a potential protein rich basal ingredient and can be used to as good feed additive if used 6% level in the diets of rohu (Labeo rohita) fry without compromising its growth performance
  • Thumbnail Image
    ThesisItemOpen Access
    “EFFECT OF PARTIAL REPLACEMENT OF FISH MEAL WITH EARTHWORM (Eisenia fetida) MEAL IN THE DIET OF Labeo rohita FRY” 3018
    (JAU, JUNAGADH, 2019-09) “EFFECT OF PARTIAL REPLACEMENT OF FISH MEAL WITH EARTHWORM (Eisenia fetida) MEAL IN THE DIET OF Labeo rohita FRY” 3018; Dr. K. H. Vadher
    The experiment on “Effect of partial replacement of fish meal with earthworm meal in the diet of Labeo rohita fry” was conducted during the September to November, 2018 in the wet laboratory of College of Fisheries Science, Junagadh Agricultural University, Veraval. The objective of the present study was to find out the effect of partial replacement of fish meal with earthworm meal in the diet of L. rohita fry. In this experiment, the data pertaining to growth, survival rate, feed conversion ratio and protein efficiency ratio of L. rohita fry at partial replacement of earthworm meal were evaluated. L. rohita fry (average weight 0.103gm) were stocked at a density of 10 nos. /aquarium in all 20 aquaria. Five experimental diets were formulated and prepared. Approximately 35% protein level was maintained in all the diets. Diet prepared without earthworm meal protein (control) and other four diets containing 5%, 10%, 15% and 20% earthworm meal protein were prepared and fed to L. rohita fry at 10% body weight two times in a day for 60 days. Highest mean weight gain, SGR, PER and lowest FCR were recorded in 10% (T3) earthworm meal protein diet. 100% Survival rate were recorded in treatment including control diet. The result of the present investigation revealed that partial replacement of earthworm meal significantly affected the mean weight gain, SGR, survival rate, FCR and PER of L. rohita fry