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  • ThesisItemOpen Access
    Efficiency of Ocimum basilicum L. (Sweet Basil) for the phytoremediation of Cd and NaCl stressed soil, with and without Jeevamrutha
    (DEPARTMENT OF BIOLOGICAL SCIENCES SAM HIGGINBOTTOM UNIVERSITY OF AGRICULTURE, TECHNOLOGY AND SCIENCES PRAYAGRAJ-211007 (U.P.) IN, 2019) Singh, Ankit Samuel; Lal, Dr. Eugenia P.
    The present study entitled “Efficiency of Ocimum basilicum L. (Sweet Basil) for the phytoremediation of Cd and NaCl stressed soil, with and without Jeevamrutha” was carried out at Department of Biological Sciences, SHUATS, Prayagraj (Allahabad) to access the potential of sweet basil, for phytoremediation of Cadmium (Cd) and sodium chloride (NaCl) stressed soil alone and in combination, with the treatment of organic liquid formulation, jeevamrutha. The results revealed that different growth, biochemical, enzymatic and non enzymatic antioxidant activities as well as essential oil percentage and cadmium accumulation in soil, roots and leaves were influenced by the treatments in both the trials. Growth attributes like germination percentage, shoot length, number of branches and number of leaves were not hindered by the least concentration of Cd and NaCl i.e. 20 ppm/kg soil and NaCl 25 mM/kg soil respectively. The combination of both the least concentrations of Cd and NaCl further influences the growth attributes of plants. Additionally, the growth attributes were negatively influenced by the higher concentrations of Cd and NaCl from 40 ppm to 80 ppm/kg soil and NaCl 50 mM to 100 mM/ kg soil and their combinations. Further, the growth attributes were slightly enhanced by the treatment of organic liquid formulation, jeevamrutha. Different biochemical attributes like chlorophyll ‘a’, chlorophyll ‘b’ and carbohydrate content were also affected by treatment of Cd, NaCl and their combinations. Chlorophyll ‘a’ as well as chlorophyll ‘b’ and carbohydrate content were reduced as the concentration of Cd and NaCl increased, both alone and in combinations. However, the plants treated with jeevamrutha showed significant enhancement in chlorophyll ‘a’, chlorophyll ‘b’ and in carbohydrate content in all the treatments. Various non-enzymatic antioxidants like carotenoid, proline and total phenol content were influenced by treatment of Cd, NaCl and their combinations. Carotenoid content showed linear decrement in different individual and combined treatments in comparison to control. However, the plants treated with jeevamrutha showed, slight enhancement in all the treatments. While proline accumulation increased with the increasing concentrations of Cd and NaCl in individual and combined treatments. Proline content was further increased by the treatment of organic liquid formulation, jeevamrutha; it shows the affirmative impact of jeevamrutha during stress 18 condition. Moreover total phenol content was negatively influenced by Cd, NaCl and their combinations, though the plants treated with jeevamrutha showed decrement in all the treatments. Furthermore, the activity of enzymatic antioxidants like catalase and peroxidase were influenced by treatments. Catalase activity decreased with the increasing concentration of NaCl and Cd in all the treatments in comparison to control. However the plants treated with jeevamrutha showed slight increment in catalase activity. Peroxidase activity was found to be higher with the rising concentration of NaCl and Cd in all the treatments. Moreover, the plants treated with jeevamrutha showed slight increase in peroxidase activity. The results for essential oil percentage demonstrated that NaCl treatments positively influenced the essential oil percentage and increment in essential oil percentage was observed with the increasing concentration of NaCl, even though Cd in low concentration could not hampered the essential oil percentage in 20 ppm/kg soil but further Cd treatments alone and in combination with NaCl causes gradual decrement in essential oil percentage of sweet basil. While, the plants treated with jeevamrutha showed slight increment in essential oil percentage in all the treatments. Accumulation of Cd, in soil was increased as the concentration of cadmium increases in the treatments. In case of roots cadmium accumulation was increased as the concentration of Cd increases in the treatments, it was further increased in combination with NaCl and jeevamrutha in all treatments. Similarly, in leaves cadmium accumulation increases with the increasing concentration in the treatments. Furthermore, the plants treated with, jeevamrutha showed slight increment in cadmium content among the treatments. It was also observed that the accumulation of cadmium was comparatively higher in roots in comparison to the leaves of sweet basil. The present study accomplished that sweet basil plants were cultivated only with the low concentrations of Cd and NaCl with organic liquid formulation, jeevamrutha in the contaminated soil, to get the better quality and yield of sweet basil crop. Relatively the higher concentration of Cd and NaCl alone and in combination reduced the growth of sweet basil plants.
  • ThesisItemOpen Access
    Efficiency of Ocimum basilicum L. (Sweet Basil) for the phytoremediation of Cd and NaCl stressed soil, with and without Jeevamrutha
    (DEPARTMENT OF BIOLOGICAL SCIENCES SAM HIGGINBOTTOM UNIVERSITY OF AGRICULTURE, TECHNOLOGY AND SCIENCES PRAYAGRAJ-211007 (U.P.) INDIA, 2019) Samuel Singh, Ankit; Lal, Dr. Eugenia P.
    The present study entitled “Efficiency of Ocimum basilicum L. (Sweet Basil) for the phytoremediation of Cd and NaCl stressed soil, with and without Jeevamrutha” was carried out at Department of Biological Sciences, SHUATS, Prayagraj (Allahabad) to access the potential of sweet basil, for phytoremediation of Cadmium (Cd) and sodium chloride (NaCl) stressed soil alone and in combination, with the treatment of organic liquid formulation, jeevamrutha. The results revealed that different growth, biochemical, enzymatic and non enzymatic antioxidant activities as well as essential oil percentage and cadmium accumulation in soil, roots and leaves were influenced by the treatments in both the trials. Growth attributes like germination percentage, shoot length, number of branches and number of leaves were not hindered by the least concentration of Cd and NaCl i.e. 20 ppm/kg soil and NaCl 25 mM/kg soil respectively. The combination of both the least concentrations of Cd and NaCl further influences the growth attributes of plants. Additionally, the growth attributes were negatively influenced by the higher concentrations of Cd and NaCl from 40 ppm to 80 ppm/kg soil and NaCl 50 mM to 100 mM/ kg soil and their combinations. Further, the growth attributes were slightly enhanced by the treatment of organic liquid formulation, jeevamrutha. Different biochemical attributes like chlorophyll ‘a’, chlorophyll ‘b’ and carbohydrate content were also affected by treatment of Cd, NaCl and their combinations. Chlorophyll ‘a’ as well as chlorophyll ‘b’ and carbohydrate content were reduced as the concentration of Cd and NaCl increased, both alone and in combinations. However, the plants treated with jeevamrutha showed significant enhancement in chlorophyll ‘a’, chlorophyll ‘b’ and in carbohydrate content in all the treatments. Various non-enzymatic antioxidants like carotenoid, proline and total phenol content were influenced by treatment of Cd, NaCl and their combinations. Carotenoid content showed linear decrement in different individual and combined treatments in comparison to control. However, the plants treated with jeevamrutha showed, slight enhancement in all the treatments. While proline accumulation increased with the increasing concentrations of Cd and NaCl in individual and combined treatments. Proline content was further increased by the treatment of organic liquid formulation, jeevamrutha; it shows the affirmative impact of jeevamrutha during stress 18 condition. Moreover total phenol content was negatively influenced by Cd, NaCl and their combinations, though the plants treated with jeevamrutha showed decrement in all the treatments. Furthermore, the activity of enzymatic antioxidants like catalase and peroxidase were influenced by treatments. Catalase activity decreased with the increasing concentration of NaCl and Cd in all the treatments in comparison to control. However the plants treated with jeevamrutha showed slight increment in catalase activity. Peroxidase activity was found to be higher with the rising concentration of NaCl and Cd in all the treatments. Moreover, the plants treated with jeevamrutha showed slight increase in peroxidase activity. The results for essential oil percentage demonstrated that NaCl treatments positively influenced the essential oil percentage and increment in essential oil percentage was observed with the increasing concentration of NaCl, even though Cd in low concentration could not hampered the essential oil percentage in 20 ppm/kg soil but further Cd treatments alone and in combination with NaCl causes gradual decrement in essential oil percentage of sweet basil. While, the plants treated with jeevamrutha showed slight increment in essential oil percentage in all the treatments. Accumulation of Cd, in soil was increased as the concentration of cadmium increases in the treatments. In case of roots cadmium accumulation was increased as the concentration of Cd increases in the treatments, it was further increased in combination with NaCl and jeevamrutha in all treatments. Similarly, in leaves cadmium accumulation increases with the increasing concentration in the treatments. Furthermore, the plants treated with, jeevamrutha showed slight increment in cadmium content among the treatments. It was also observed that the accumulation of cadmium was comparatively higher in roots in comparison to the leaves of sweet basil. The present study accomplished that sweet basil plants were cultivated only with the low concentrations of Cd and NaCl with organic liquid formulation, jeevamrutha in the contaminated soil, to get the better quality and yield of sweet basil crop. Relatively the higher concentration of Cd and NaCl alone and in combination reduced the growth of sweet basil plants.
  • ThesisItemOpen Access
    Assessment of growth, yield and oil quality of mustard varieties (Brassica juncea L.) as influenced by nutrient management system
    (Department of Biological Sciences, Faculty of Science, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (Allahabad), U.P., India-211007, 2019) BEENISH, OBIDA; Lal, Dr. Eugenia P.
    A field experiment was conducted at the Central Research Field of Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during 2016-17 and 2017-18 to study the “Assessment of growth, yield and oil quality of mustard varieties (Brassica juncea L.) as influenced by nutrient management system’’. The experiment was laid out in a Randomized block design with three replications, the factors being, organic manures and biofertilizers with 10 different treatment combinations and five different varieties of mustard. The result of the study revealed that the variety Rani with T6 (50% RDF + vermicompost + Azotobacter) exhibited significantly higher in plant growth, yield, oil yield and quality parameters viz., plant height (142.1, 143.3 and 142.70 cm), number of primary branches (6.98, 7.15 and 6.71 plant-1), the treatment T4 (50% RDF + FMY + Azotobacter) showed higher number of secondary branches (6.26, 6.86 and 6.16 plant-1). Flower appearance (DAS) was also recorded higher in treatment T6 (50% RDF + vermicompost + Azotobacter) number of siliquae (150.4, 151.5 and 150.67 plant-1), number of seeds (9.95, 10.09 and 9.88 siliquae-1), 1000-seed weight (4.46, 4.47 and 4.47 g), seed yield (12.16, 12.45 and 12.31 q ha-1), stover yield (32.92, 33.74 and 32.69 q ha-1). The harvest index showed maximum in Treatment T7 (50% RDF + Vermicompost + PSB) (27.61, 28.16 and 27.89 %). Treatment T6 (50% RDF + vermicompost + Azotobacter) showed higher Biological yield (45.08, 45.32 and 45.20), nitrogen (N) content in seed (3.24, 3.23 and 3.11 %), protein content in seed (20.25, 20.19 and 20.22 %), protein yield (246.24, 251.36 and 249.25 kg ha-1), oil content (38.66, 38.60 and 38.24 %).Oil yield was showed maximum in treatment T7 (50% RDF + Vermicompost + PSB) (437.63, 443.21 and 458.46 kg ha-1). Treatment T1 (RDF) showed higher concentration of palmitic acid (6.83, 6.87 and 6.15 %), stearic acid (4.46, 4.52 and 4.49 %). Treatment T3 (75% RDF + Vermicompost) showed higher concentration of oleic acid (38.60, 38.62 and 38.04 %). Linoleic acid was showed maximum in treatment T8 (25% RDF + FYM + Azotobacter + PSB) (49.33, 49.36 and 49.35 %). Treatment T6 (50% RDF + vermicompost + Azotobacter) showed maximum amount linolenic acid (0.33, 0.34 and 0.33 %), arachidic acid (0.93, 0.94 and 0.94 %), chlorophyll content (3.09, 3.10 and 3.10 mg g-1 f. wt), Total Soluble Sugar (11.92, 11.95 and 11.94 mg g-1 fw.t) and proline (11.40, 11.25 and 11.33 mg g-1 fw.t). It was also evident from the results that the plant growth, xvii yield, oil yield and quality parameters as recorded by different treatments during 2016-17 were comparatively lower than recorded during 2017-18.
  • ThesisItemOpen Access
    Sodium Chloride and Cadmium Stresses Mitigation by Application of Salicylic Acid and Indole Acetic Acid in Tomato (Lycopersicum esculentum L.) var., PKM-1 and
    (Department of Biological Sciences, Faculty of Science, Sam Higginbottom University of Agriculture, Technology & Sciences Prayagraj – 211007, 2019) Malik, Zahid Ahmad; Lal, Dr. Eugenia P.
  • ThesisItemOpen Access
    STUDIES ON ANTIFUNGAL PROPERTIES OF LICHENS WITH SPECIAL REFERENCE TO PLANT PATHOGENS
    (Department of Biological Sciences Sam Higginbottom University of Agriculture, Technology and Sciences (Formerly Allahabad Agricultural Institute) Allahabad-211007, U.P. (India, 2019) Babiah, Preeti Sarah; John, Dr. Suchit A.
    Lichens, a symbiotic phenotype of nutritionally specialized fungi with algae, are an outstandingly successful group, exploiting a wide range of habitat throughout the world. They have varied chemistry and produce unique secondary compounds most of which are not known from other groups of plants. They are highly significant and their utilization in folklore as medicine has been cited in different pharmacopies of the world. They have been recognized for their antibiotic, antimicrobial, antioxidant, cytotoxic activities. The biological activities of lichens is mostly due to the presence of peculiar secondary metabolites present in them. The present study focuses on the screening of twelve medicinally important lichen species for the investigation of their antifungal properties against five strains of plant pathogenic fungi causing rot and wilt diseases in most of the common edible crop plants. The compounds were extracted from lichen species in different polarity of solvents to determine the best extracting solvent for lichen species and the antifungal property was determined by discdiffusion method and broth tube dilution method. The potential species showing activity were subjected for establishment of mycobiont cultures. The mycobiont being the dominant partner in lichenization, and responsible for production of metabolites was investigated for its antifungal potential by isolating it from photobiont for the purpose of establishing axenic cultures and for obtaining desired metabolites in high amounts. The species Heterodermia diademata, Heterodermia leucomelos, Usnea ghattensis showed highest inhibitory fungicidal efficiency against the pathogens when extracted in acetone which was more than the synthetic control ketoconazole. The cultures of these three species were established successfully and high biomass production was estimated in modified malt yeast agar media with 10% additional sucrose and the production of similar compounds as natural thalli was observed. The antifungal activity by cultures was recorded but much less than the natural thalli and standard control. The observations reveal the remarkable antifungal properties found in lichen species and suggests their utilization as natural fungicidal agents in place of synthetic ones for the benefit of humans, plants and environment.
  • ThesisItemOpen Access
    Effect of different levels of NaCl and CaCl2 on tomato (Lycopersicon esculentum Mill.) variety GS-600
    (Department of Biological Sciences Sam Higginbottom University of Agriculture, Technology & Sciences (Formerly Allahabad Agricultural Institute) Allahabad, U.P, 211007, 2019) Ibrahim, Fouzi A; John, Dr. Suprit John
    Thе еxpеrimеnt еntitlеd ―Еffеct of diffеrеnt lеvеl of NaCl and CaCl2 on tomato (Lycopеrsicon еsculеntum Mill) variety GS-600‖ was carriеd out separately during the rabi season in 2014-2015 and 2015-16. The experiment was conducted with RBD design with 16 treatments and 3 replications. Different concentrations of NaCl and CaCl2 were used during the study. The result releaved that both the NаCl аnd CаCl2 had significant effect on growth and yield of tomato. The increasing levels of NаCl had negative effect on growth and yield whereas the different level of CаCl2 showed positive effect. Similar results have been observed for biochemical parameters. It was clear from the study that calcium chloride could be used to mitigate the stress of sodium chloride
  • ThesisItemOpen Access
    Impact of Eichhornia Treated Sewage Water on the Growth and Yield of Potato crop (Solanum tuberosum L.)
    (Department of Biological Sciences Sam Higginbottom University of Agriculture, Technology & Sciences Allahabad, U.P - 211007, 2018) Khare, Ani; Lal, Dr. (Mrs.) Eugenia. P.
    Due to constraint in availability of fresh water for irrigation, waste water especially sewage water is being increasingly used for irrigation of agricultural fields. However, Sewage water contains soluble salts and heavy metals that accumulate in the root zone with possible harmful effects on soil health and crop yield. A research study was conducted to analyze the impact of sewage water treated with Eichhornia crassipes on the growth and yield of potato crop (Solanum tuberosum L.) and on the soil health. For the purification of the sewage water, Eichhornia crassipes commonly known as water hyacinth was cultured in a tank of 5ft/4ft/2.5ft dimension. The physico-chemical parameters studied were pH, EC, DO, BOD, COD, Turbidity, TDS, N, P, K and heavy metals. Two years of investigation showed that there is marked reduction in BOD (64.17%), COD (81.47%), Turbidity (93.28%) and other physicochemical parameters. Heavy metal parameters were also significantly reduced. At the same time it was found that DO of sewage water was substantially increased (538%) after 21 days of treatment. . The growth parameters like plant height, no. of branches, no. of leaves etc. and nutrient and heavy metals concentration were found maximum in potato crop when irrigated with sewage water in comparison to Eichhornia treated sewage water while potato crop yield was found maximum in sewage water treated with water hyacinth. The soil samples were analyzed for pH, EC, % Organic carbon, N, P, K and heavy metals (Cd, Ni, Cr and Pb). An appreciable increase in organic carbon, available N, P, K and heavy metals was recorded in the sewage irrigated soil over that of biologically treated sewage water. The concentration of heavy metals was higher in sewage irrigated soil as compared to other treatments. The average contents of Ni, Cd, Cr and Pb in sewage irrigated soil were 0.697, 0.015, 6.160 and 0.115 in 2010-11 and 0.711, 0.025, 6.512 and 0.146 in 2011-12 respectively. It is concluded here that Eichhornia helps in increasing yield of potato crop without deteriorating crop & soil health thus it is justifiable to use Eichhornia plant as a low cost, ecofriendly and effective source of treatment of sewage water.
  • ThesisItemOpen Access
    Assessment of Plant Species for Rehabilitation of Waste Land in Meja Tehsil
    (Department of Biological Sciences Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211007 2018, 2018) LAL, SUKH
    Tree and forest were always considered as an integral part of Indian culture. This is amply supported by the ancient scriptures and historical records. Fertilizer and irrigation are important inputs to increase productivity of forest plantation. FYM in different proportion are generally used to increases the leaf area of plants due to the effect of available Nitrogen. This increases the photosynthetic surface and thus promotes growth. The experiments were conducted at village Bhasunder kala and Bagaha as two different sites I & II of Allahabad district in November 2011, to find out the effect of irrigation schedule and different organic manure on assessment of plant height, collar girth and survival percentage of seedling of Pongamia pinnata, Emblica officinalis, Carrisca carandus..The experiments consist of three different age group of plants, given with different treatments of organic manure and different time interval of irrigation to study the seedling growth and the survival of plant. Plantation was done on both sites Rocky and Usar. Twelve treatments and three different organic manure were applied with three irrigation regime (viz.10 Days irrigation, 20 Days irrigation, 30 Days irrigation.). Organic manure was given in quarterly dose in each treatment. All three replication with species Pongamia pinnata, Emblica officinalis, and Carrisca carandus, the best survival performance was given by species P. pinnata . In six month old seedling of P. pinnata best treatment T10 (rocky-117.10cm and usar-108.34cm) with best rocky site. In one year old seedling treatment T10 (rocky-170.60cm and usar-139.78cm) and two year old seedling treatment T10 (rocky-193.67cm and usar-156.33cm).In view on age group two year old seedling gave a best growth increment overall treatment and age group with organic manure and irrigation regime.
  • ThesisItemOpen Access
    EFFECT OF BIOFERTILIZERS ON GROWTH AND YIELD OF SUNFLOWER (Helianthus annuus L.)UNDER INDIA AND LIBYA NATURAL CONDITIONS
    (Department of Biological Science Faculty of science Sam Higginbottom University of Agriculture, Technology &Sciences Allahabad – 211007 (UP) 2018, 2018) ABDASLAM, SANA; Lal, Dr. (Mrs.) Eugenia P.
    The experiment was conducted at the Department of Biological Sciences, SHI ATS. Allahabad. Department of Botany. Faculty of Science (Zintan), University of El-Jabal Wl-Gharby, Libya during year 2013- 15. The experiment was laid out in Randomized Block Design (RBD). There were eleven treatments including control, replicated 3 times in which several fertilizer application control (FYM % 2.51 t/ha, Vermicompost @ 2.5 t kg, Trichoderma harizanum @10g;kg), In the view of present investigation the most effective growth treatment was T3-(FYM @ 12.5 t/ha + vermicompost @ 2.5 t„ha),compared to control and other treatments was found to be the best in terms of plant height, number of leaves, number flower, Number of filled seed per capita. Weight of dry flowers (g), and Oil content (%).As far as planting condition concerned. Indian emerged superior in compare to Libyan condition.