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20122
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Subject: Animal Reproduction and Gynecology × End date: 2012 × Start date: 2012 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    EFFECT OF GnRH ON CONCEPTION RATE IN REPEAT BREEDER GRADED MURRAH BUFFALOES
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-06) VENKAT RAO, B; CHANDRA SHEKAR REDDY, K(MAJOR); SADASIVA RAO, K; PURUSHOTHAM, G
    ABSTRACT: The present investigation “Effect of GnRH on conception rate in repeat breeder graded Murrah buffaloes” was taken up on 86 repeat breeder buffaloes to study the incidence of repeat breeding and various aspects of estrous cycle. The efficacy of GnRH protocols (10μg or 2.5 ml, 20μg or 5.0 ml given 8 hours before AI and on day 12 post AI either once or twice in different treatment groups) for enhancement of fertility was studied in 70 repeat breeding graded Murrah buffaloes. The estrous cycle length in six treatment groups was 21.6±0.69, 20.1±0.43, 20.3±0.58, 21.5±0.34, 21.3±0.54, and 20.2±0.47. The estrus duration in six treatment groups were 23.8±0.36, 24.5±0.31, 23.4±0.48, 24.2±0.47, 23.6±0.40, and 24.3±0.30 and the estrus intensity were 13.2±0.36, 14.1±0.28, 13.7±0.21, 14.1±0.31, 13.1±0.31, 13.2±0.25 days in group 1, group 2, group 3,group 4, group 5, group 6, respectively. In control groups the length of estrous cycle, duration of estrus and intensity of estrus were 21.5±0.60 days, 22.2±0.36 hours and 12.9±0.18 points, respectively. Normal estrus pattern was observed in 87.20 per cent of repeat breeder buffaloes. The repeat breeder buffaloes were randomly divided into seven groups (each group consisting of 10 buffaloes). The repeat breeding buffaloes in group 1 and 2 were treated with 10 μg or 2.5 ml and 20 μg or 5 ml of GnRH (Buserlin) 8 hours before AI, respectively. In group 3 and 4 were treated with 10 μg or 2.5 ml and 20 μg or 5 ml of GnRH on day 12 post AI respectively. Group 5 were treated with 10 μg or 2.5 ml of GnRH (Buserlin) 8 hours before AI and on day 12 of post AI. Group 6 were treated with 20 μg or 5 ml of GnRH (Buserlin) 8 hours before AI and on day 12 of post AI. Group 7 were consisted of untreated control buffaloes. The conception rates of repeat breeding graded Murrah buffaloes in group 1, 2, 3, 4, 5, 6 and control group were found to be 50.00, 60.00, 40.00, 40.00, 50.00, 60.00 and 30.00 per cent, respectively. It was concluded that all treatments significantly (P < 0.05) enhanced the conception in repeat breeder buffaloes when compared to control buffaloes. The GnRH could be effectively used for higher conception rates in repeat breeding buffaloes.
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    ThesisItemOpen Access
    EFFECT OF MINERAL SUPPLEMENTATION IN POST PARTUM ANOESTRUS GRADED MURRAH BUFFALOES
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-07) GOPALA KRISHNA, M.V.; RAM CHANDRA REDDY, K(MAJOR); SADASIVA RAO, K; GIREESH KUMAR, V; KISHAN KUMAR, M
    ABSTRACT: The present investigation ―Effect of mineral supplementation in post partum anoestrus graded Murrah buffaloes‖ was undertaken in post partum pluriparous true anoestrus buffaloes (above 2 months) after calving, having a body condition score ranging from 3.0 to 4.0 screened clinically for reproductive disorders. 60 animals free from reproductive problems and having completely involuted uterus were selected for the study. Animals were divided into 4 groups comprising 15 in each group and subjected to different mineral supplementation (Group-I: Cyclomin-7 at the dose rate of 2boli/week/animal and Dicalcium phosphate at the dose rate of 30 gm/day/animal, Group-II: Non-chelated minerals at the dose rate of 50 gm/day/animal, Group-III: Chelated minerals at the dose rate of 50 gm/day/animal, Group-IV: Control group, without any mineral supplementation) protocols for 30 days and were fed with dry fodder, green fodder and concentrates as per the production requirements in intensive feeding system. During the course of treatment estrus symptoms were monitored with the help of teaser bulls twice or thrice per day and by visual observation 5 to 6 times per day. Animals which were not showing estrus symptoms were examined once in a week per rectally to know the ovarian activity. Animals which showed estrus symptoms have been inseminated twice during late estrus period. After breeding, animals were examined for subsequent estrus cycle and inseminated if required during subsequent cycles. Pregnancy Diagnosis was done per rectally after 50 days of breeding to know the conception status. Blood samples have been collected on zero, 15th, 30th days of treatment for estimation of serum minerals and haematological parameters. Estrus intensity and duration of estrus has been recorded based on the physiological symptoms. The mean haemoglobin levels on zero, 15th and 30th day in group I were 11.05 ± 0.26, 11.31 ± 0.27 and 11.55 ± 0.27, in group II were 11.01 ± 0.22, 11.32 ± 0.23 and 11.63 ± 0.22, in group III were 11.03 ± 0.18, 11.46 ± 0.26, and 11.89 ± 0.27 and in group IV were 11.01 ± 0.16, 11.03 ± 0.17 and 11.06 ± 0.17 g% respectively. Haemoglobin levels on 15th and 30th day of treatment group III was significantly (p < 0.05) higher. In between groups there was no significant difference in haemoglobin levels on zero, 15th day. But on 30th day three treatment groups were having statistically similar concentrations which were significantly (p < 0.05) higher than the control group. The mean Serum calcium levels on zero, 15th and 30th day were 7.20 ± 0.23, 7.69 ± 0.23 and 8.08 ± 0.22 in group I, 7.15 ± 0.21, 7.85 ± 0.12 and 8.63 ± 0.16 in group II, 7.24 ± 0.23, 7.97 ± 0.12 and 8.79 ± 0.07 in group III and 7.31 ± 0.10, 7.34 ± 0.06 and 7.35 ± 0.07 mg/dl in group IV respectively. On 30th day of treatment the serum calcium levels were higher in group III followed by group II, group I and lowest in group IV. The mean Serum phosphorus levels on zero, 15th and 30th day in group I were 5.46 ± 0.29, 5.79 ± 0.32 and 6.24 ± 0.18 mg/dl, in group II were 5.78 ± 0.18, 5.87 ± 0.28 and 6.30 ± 0.25 mg/dl, in group III were 5.56 ± 0.07, 5.96 ± 0.17 and 6.94 ± 0.05 mg/dl and in group IV were 5.56 ± 0.07, 5.83 ± 0.11 and 5.80 ± 0.10 mg/dl respectively. On 30th day of treatment the serum phosphorus level was significantly (p < 0.05) higher in group III followed by group II, I and IV. The mean Serum iron levels on zero, 15th and 30th day in group I were 2.716 ± 0.112, 3.122 ± 0.084 and 3.180 ± 0.110, in group II were 2.656 ± 0.058, 3.147 ± 0.068 and 3.246 ± 0.140, in group III were 2.689 ± 0.047, 3.174 ± 0.194, and 3.680 ± 0.081 and in group IV were 2.679 ± 0.039, 2.690 ± 0.061 and 2.702 ± 0.060 ppm respectively. Serum iron levels in group I, II were significantly (p < 0.05) lower on zero day when compare to 15th and 30th day. In between four groups, on 30th day group III was having significantly (p < 0.05) higher Iron levels. The mean Serum copper levels on zero, 15th and 30th day were 0.047 ± 0.003, 0.097 ± 0.003 and 0.142 ± 0.006 ppm in group I, in group II were 0.049 ± 0.005, 0.127 ± 0.005and 0.163 ± 0.005, in group III were 0.052 ± 0.002, 0.149 ± 0.003 and 0.184 ± 0.005 and in group IV were 0.049 ± 0.002, 0.052 ± 0.002 and 0.054 ± 0.003 ppm respectively. The serum copper levels were significantly (p < 0.05) higher on 30th day followed by 15th day and lowest on zero day in all three treatment groups. In between four groups there was significant difference (p < 0.05) on 15th and 30th day and group III was having significantly (p < 0.05) higher serum copper levels. The mean serum manganese levels on zero, 15th and 30th day in group I were 0.056 ± 0.003, 0.076 ± 0.003 and 0.151 ± 0.007 ppm, in group II were 0.058 ± 0.002, 0.112 ± 0.002 and 0.165 ± 0.006 ppm, in group III were 0.054 ± 0.002, 0.148 ± 0.002 and 0.182 ± 0.002 ppm and in group IV were 0.056 ± 0.002, 0.056 ± 0.003 and 0.055 ± 0.003 ppm respectively. The serum manganese levels were significantly (p < 0.05) different in all three treatment groups on zero, 15th, 30th day. In between four groups there was significant (p < 0.05) difference on 15th and 30th day and group III was having higher serum manganese levels. The mean serum zinc levels on zero, 15th and 30th day in group I were 1.062 ± 0.023, 1.162 ± 0.033 and 2.639 ± 0.061, in group II were 1.089 ± 0.008, 1.248 ± 0.018 and 3.038 ± 0.017, in group III were 1.075 ± 0.010, 1.427 ± 0.039 and 3.767 ± 0.042 and in group IV were 1.032 ± 0.012, 1.050 ± 0.010 and 1.054 ± 0.010 ppm respectively. On 15th and 30th day group III was having significantly (p < 0.05) higher serum zinc level followed by II, I and IV. The percentage of estrus response was 46.66 per cent, 66.66 per cent, 86.66 per cent and 33 per cent; mean duration of estrus was 22.13 ± 0.83, 26.46 ± 0.98, 32.93 ± 0.81 and 15.80 ± 0.65 hours; the intensity of estrus was 10.57 ± 0.69, 12.30 ± 0.76, 13.15 ± 0.72 and 11.4 ± 1.36 points; the interval between treatments to the estrus exhibition 46.67 ± 0.95, 35.20 ± 1.06, 25.0.98 and 70.13 ± 1.79 days and conception rates were 20 per cent, 40 per cent, 73.33 per cent and 13.33 per cent in group I, II, III and IV respectively. Regarding estrus response, duration of estrus and estrus intensity there was significant (p < 0.05) difference in between all four groups and group III was having significantly (p < 0.05) higher and followed by group II, I and lowest in IV. The interval between treatments to the estrus exhibition there was significant (p < 0.05) difference in between all four groups, group III was having significantly (p < 0.05) lesser interval next higher in group II, I and highest in group IV. The conception rates were significantly (p < 0.05) higher in group III and followed by group II and I lowest in IV. Based on the results of this study come to the concluded that due to the supplementation of chelated mineral mixture, the mean serum mineral levels and bioavailability increased more than non chelated mineral mixture and Di Calcium phosphate plus Cyclomin – 7. Consequently it enhanced the estrus response, duration of estrus and estrus intensity and decreased the interval between the treatment to the estrus exhibition and increased the conception rate in post partum anoestrus graded Murrah buffaloes.