Tamil Nadu Veterinary and Animal Sciences University, Chennai
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Browsing Tamil Nadu Veterinary and Animal Sciences University, Chennai by Subject "Animal Reproduction and Gynaecology and Obstetrics"
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ThesisItem Open Access Androgen Deprivation Therapy of Benign Prostatic Hyperplasia in Dogs Using Osaterone Acetate(TANUVAS, 2010) Divya, T.R.; TANUVAS; Sridevi, P; Kulasekar, K; Kumarasamy, PThesisItem Open Access Comparison of Slow Freezing and Open Pulled Straw Vitrification Methods for Cryopreservation of Bovine Embryos(TANUVAS, 2008) Ajmal, C.H.; TANUVAS; Rajasundaram, R.C.; Subramanian, A.; Palanisamy, A.ThesisItem Open Access Conception Rate Following Oestrus Induction with CIDR in Buffaloes Treated for Retained Fetal Membranes(TANUVAS, 2013) Ganesh, K; TANUVAS; Selvaraju, M; Nepolean, R. Ezakial; Doraisamy, K.A.ThesisItem Open Access Conception Rate Following Ovsynch Protocol in Cows Treated for Retained Fetal Membranes(TANUVAS, 2013) Velladurai, C; TANUVAS; Nepolean, R. Ezakial; Selvaraju, M; Doraisamy, K.A.ThesisItem Open Access Development Competence and Gene Expression Pattern of In Vitro Fertilized and Parthenogenetically Activated Preimplantation Ovine Embryos(TANUVAS, 2011) Raja, S; TANUVAS; Balasubramanian, S; Veerapandian, C; Raj, G. DhinakarThesisItem Open Access Effect of Serum Free Media on In Vitro Maturation, Fertilization and Embryo culture of Ovine Oocytes(TANUVAS, 2010) Suresh, S; TANUVAS; Kulasekar, K; Devanathan, T.G.; Vairamuthu, SThesisItem Open Access Efficacy of Aglepristone and Prostaglandin F2α in Treatment of Open Cervix Pyometra in Bitches(TANUVAS, 2011) Thirumurugan, K; TANUVAS; Rajasundaram, R.C.; Veerapandian, C; Vairamuthu, SThesisItem Open Access Estrus Synchronization in Goats (Capra hircus) with Triu C® and Prostaglandin F2α(TANUVAS, 2012) Indhumathi, B; TANUVAS; Joseph, Cecilia; Kulasekar, K; Sivaselvam, S.N.ThesisItem Open Access Luteolytic Effect of Prostaglandin F2 α At Different Doses in Cyclic Crossbred Cows(TANUVAS, 2010) Umeshboopalan, G; TANUVAS; Asokan, S.A.; Subramanian, A; Subramanian, AThesisItem Open Access Supplementation of Nitric Oxide Inhibitor During In vitro Maturation and Culture on Subsequent Embryo Development in Ovine(TANUVAS, 2008) Priyadharsini, R.; TANUVAS; Devanathan, T.G.; Subramanian, A.; Thangavel, A.ThesisItem Open Access Synchronization of Ovulation in Postpartum Buffaloes during Peak and Low Breeding Seasons using Ovsynch with CIDR Protocols(Tamil Nadu Veterinary and Animal Sciences University, 2014) Ravikumar, K.; TANUVAS; Nepolean, R. EzakialSynchronization of ovulation is a recent reproductive tool to augment fertility in bovines. However, efficacies of various combinations of Ovsynch protocol with presynch or CIDR or mineral mixture during peak and low breeding seasons on conception rate in buffaloes have not been reported in detail. A total number of 140 graded Murrah buffaloes were randomly and equally divided into seven experimental groups viz. Groups I to VI (Treatment groups) and Group VII (Control group) during peak breeding season (PBS; between October and March) and low breeding season (LBS; between April and September). Each group comprised of 10 buffaloes. Buffaloes of groups II, IV, V, VI and VII were supplemented orally with TANUVAS mineral mixture at the dose rate of 50 gms per day per buffalo for 15 days from the day of selection. Buffaloes of group I and II were treated with Ovsynch protocol (10 μg of GnRH on day 0, 500 μg of PGF2α on day 7 and another 10 μg of GnRH 48 hours after (day 9) the PGF2α) and timed artificial insemination at 16 to 18 hours after (day 10) the second GnRH injection). In groups III and IV, in addition to the Ovsynch treatment, CIDR was inserted intra-vaginally for 7 days from day 0 to day 7 of Ovsynch in all the buffaloes. In group V buffaloes, 8 days before the start of Ovsynch treatment, 500 μg of PGF2α was injected intramuscularly to all the buffaloes (- d 8). Two days later (- d 6), each buffalo was given intramuscularly 10 μg of GnRH (I^st GnRH) and Ovsynch protocol was started 6 days after the first GnRH injection (d 0). The treatment procedures of group V were followed in the buffaloes of group VI. Additionally during the Ovsynch treatment from d 0 to d 7 all the buffaloes were treated with CIDR intra-vaginally. Group VII buffaloes (control) were observed for the signs of estrus for 30 days from the time of selection and artificial insemination was done during the observed estrus. Buffaloes belonging to groups I to VI was bred by timed artificial insemination (TAI) at 16-18 hours after the last GnRH injection and at observed estrus in group VII. Those animals which failed to conceive and returned to estrus following TAI were subjected to artificial insemination in the subsequent estrus. All the buffaloes (100 per cent) of groups III, IV and VI in PBS and LBS retained the CIDR from the time of insertion to withdrawal. In experimental groups (I to VI) all the buffaloes during PBS and LBS exhibited estrus (100 per cent) following the synchronization of ovulation protocols. The mean (±SE) interval to onset of induced estrus and duration of induced estrus in groups I to VII of PBS was significantly different (P≤0.05) from the corresponding group of LBS. The overall percentage of intense estrus intensity following synchronization of ovulation in buffaloes during PBS was high (20.00 per cent) when compared to the buffaloes treated during LBS (5.71 per cent). The overall percentage of weak estrus intensity in buffaloes during PBS was low (24.28) when compared to LBS (45.71 per cent). The percentages of ovulatory response following synchronization of ovulation on day 10 post AI in buffaloes were 80.00, 90.00, 100.00, 100.00, 100.00, 100.00 and 40.00 per cent, respectively during PBS and the values in LBS were 60.00, 70.00, 100.00, 100.00, 100.00, 100.00 and 30.00 per cent, in groups I, II, III, IV, V, VI and VII, respectively. The overall ovulatory response in experimental groups during PBS and LBS was 87.14 and 80.00 per cent, respectively. During PBS, the percentages of first service, second service and overall first and second service conception rates observed were 20.00, 30.00 and 50.00 in group I; 30.00, 20.00 and 50.00 in group II; 30.00, 30.00 and 60.00 in group III; 30.00, 40.00 and 70.00 in group IV; 40.00, 30.00 and 70.00 in group V; 50.00, 30.00 and 80.00 in group VI and 20.00, 10.00 and 30.00 per cent in group VII, respectively. The percentages of first service, second service and overall first and second service conception rates in groups I, II, III, IV, V, VI and VII during LBS were 10.00, 20.00 and 30.00; 20.00, 10.00 and 30.00; 20.00, 20.00 and 40.00; 30.00, 20.00 and 50.00; 20.00, 30.00 and 50.00; 30.00, 30.00 and 60.00 and 10.00, 10.00 and 20.00 per cent, respectively. Synchronization of ovulation programmes in postpartum buffaloes yielded 58.56 and 40.00 per cent overall conception rate during PBS and LBS, respectively. The mean (±SE) number of AI cases per month presented at Veterinary College and Research Institute Artificial Insemination Unit, Namakkal during PBS significantly differed from LBS every year. Further, there was a drastic reduction in number of buffaloes brought for AI from 2003 to 2013 both during PBS and LBS. THI during 2012 and 2013 indicated that buffaloes were exposed to heat stress not only during LBS but also during PBS. The mean blood glucose levels were higher in all the groups (I to VII) on all days of blood collections in LBS than PBS. The blood glucose and serum total protein, total cholesterol, triglycerides, calcium and phosphorus levels were increased from selection to 10 days post AI in all the groups of PBS and LBS. The serum total protein, cholesterol, calcium and phosphorus levels were higher in all the groups in PBS than in LBS. All the groups of PBS and LBS had more than 1 ng/ml of mean serum progesterone at the time of selection. In presynch groups, (V and VI) the mean serum progesterone levels on d -8, was more than 1.40±0.14 ng/ml. All the groups of PBS and LBS had below 0.57±0.06 ng/ml of mean blood progesterone on day 10 (induced estrus). The mean serum concentration of progesterone in buffaloes at 10 days post AI ranged from 2.13±0.05 to 4.72±0.32 ng/ml in PBS and LBS. The serum estradiol-17β concentrations ranged from 13.67±1.41 to 37.68±1.04 pg/ml during different phases of treatments in PBS and LBS. On d -6 and d 10 (induced estrus) the serum estradiol-17β was above 28 ng/ml. Serum estradiol-17β level was found to be lower in all the groups of LBS than PBS. The cost per overall first and second service conception in groups I, II, III, IV, V, VI and VII in PBS was Rs.7,410.00, 7,492.00, 6,425.00, 4,637.00, 3,708.00, 3,432.00 and 15,304.00 and in LBS, it was Rs.16,683.00, 16,821.00, 12,887.00, 9,092.00, 7,792.00, 6,744.00 and 26,206, respectively. The cost per conception was lower in group VI of PBS and LBS than other groups. Hence, it is concluded that presynch + Ovsynch + CIDR + mineral mixture protocol is recommended to achieve maximum conception rate in buffaloes under field conditions.