Browsing by Author "Bhoyar, P.B."

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    Studies on miRNA Expression in Peste des-petits ruminants virus and Genes Associated with Immune Response and Immunity
    (NVC, Nagpur, 2019-08-19) Bhoyar, P.B.; Tembhurne, P. A.
    PPR is an acute viral infection of small ruminants viz, sheep and goat, caused by Small Ruminant Morbillivirus (SRMV) also knows as a Peste-des-petitis ruminant virus (PPRV) belonging to morbillivirus genus of family Paramyxovirdae. During PPR disease, virus modulates host transcriptome as well as small RNA population for it’s replication or overcome immunity of host resulting in setting up the disease and thus affecting host. So, host-virus interaction needs to be explored. Hence, the present study was planned for profiling of miRNAs and the genes associated with innate immunity/ immune response of PPRV infected animals. The PPRV was confirmed from infected and vaccinated animals by M gene based RTPCR. The expression of miRNA’s miR-486-5p, mir-211 and genes IL18RAP, ISG20, were analyzed in the PBMC samples by qRT-PCR in infected, vaccinated and PPR negative apparently healthy goats. The study showed that Chi-miR-486-5p was up regulated upto 5.34 fold in infected group and also in vaccinated group upto 3.07 fold in comparison with control group. The Chi-miR-211 was found to be down regulated upto 0.39 in infected group and was slightly up regulated upto 1.39 in vaccinated group. On the other hand, the target genes associated with innate immunity and immune responses IL18RAP was up regulated upto 4.29 fold (in 3 infected) and 1.83 fold (in 2 infected) in infected animals, similarly ISG20 was found to up regulated upto 3.49 fold (in 3 infected) and 1.47 fold (in 2 infected) whereas in vaccinated animals the both gene were down regulated, IL18RAP was down regulated upto 0.23 fold (in 3 infected) and 0.64 fold (in 2 infected) and ISG20 gene was down regulated upto 0.64 fold (in 3 infected) and 0.60 fold (in 2 infected) as compared to PPRV negative apparently control group. So, current study concludes that, Chi-miR-486-5p, chi-mir-211 and IL18RAP, ISG20 plays the significant role in pathogenesis and host antiviral immune response in PPRV infection in goats and could be used as future biomarkers for disease prognosis.