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Browsing Thesis by Author "Ade, M. J."

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    Molecular Characterization of Bovine Rotaviruses from Amravati Region of Maharashtra
    (MAFSU, Nagpur, 2018) Ade, M. J.; Ingle, V. C.
    The present research work was aimed to study incidence of rotaviruses among cattle and buffalo calves in Amravati Region of Maharashtra state using conventional methods and to characterize using molecular tools. In the present study 288 fecal samples from diarrheic and non-diarrheic bovine calves, (0-6 months and above 6 months) were screened for the presence of Rota viruses by latex agglutination test. A total of 35 fecal samples found positive by latex agglutination test from bovine calves, were subjected to Ribonucleic acid-polyacrylamide gel electrophoresis (RNA-PAGE) analysis and further screened for the detection of group A Rotavirus by VP4 gene based RT–PCR assay. Of total 288 fecal samples screened 35 samples were found positive by latex agglutination test indicating overall per cent positivity of 12.15% where 14.14% positivity was observed in cattle calves and 7.77% was in buffalo calves. From the 198 fecal samples of cattle calves, an incidence of 23.47% (27/115) was recorded in diarrheic and of 1.20% (1/83) in non-diarrheic animals. Similarly an incidence of 14.28% (7/49) was recorded in diarrheic buffalo calves indicating overall positivity of 20.73% in diarrheic animals. None of the non-diarrheic buffalo calves could show presence of the Rota virus in fecal samples by Latex Agglutination test. The district wise incidence indicated highest positivity in Yavatmal district 20.63% (13/63) followed by Amravati 16.00% (8/50), Akola 13.33% (8/60) Buldhana 6.77% (4/59) and Washim 3.57% (2/56). In the present study the incidence of 14.50% and 8.04%was recorded in the cattle and buffalo calves of 0 to 6 months age respectively. Whereas, no positivity was recorded in calves of age of above 6 months. An incidence of 22.61% and 13.51%was recorded in the cattle and buffalo male calves and 7.89% and 3.77% in female cattle and buffalocalvesrespectively. One out of 35 fecal samples (2.85%) of cattle calves of 0-6 month found to be positive for rotavirus typical RNA-PAGE with 11 segments having typical migration pattern 4:2:3:2. In this study one diarrheic fecal sample from cattle calf found positive in RNA-PAGE was also found positive by VP4 gene based RT–PCR indicating presence of group A rota virus. From the present study it is concluded that the latex agglutination test could be useful for the preliminary screening of Rota virus in the fecal samples. The incidence was more in male diarrheic calves of below 6 months of age.