Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/96286
Authors: Divya Bhagat
Title: haracterization of potential Mesorhizobium sp. for functionality traits in chickpea (Cicer arietinum L.)
Publisher: Punjab Agricultural University
Language: en
Type: Thesis
Agrotags: planting
root nodulation
Abstract: The present investigation was carried out to characterize native Mesorhizobium sp. for functional and antagonistic traits in chickpea (Cicer arietinum L.). Sixteen Mesorhizobium sp. were isolated from chickpea growing areas of Punjab. All the isolates of Mesorhizobium sp. were characterized on the basis of morphological, biochemical, functional traits and plant infectivity test. Significantly high IAA was produced with isolates of Mesorhizobium sp. LGR 16, LGR 15 and reference Mesorhizobium sp. LGR 33 in the range of 44.49 – 48.69 µg mL-1. Of 17 isolates, 14 mesorhizobia including reference Mesorhizobium sp. LGR 33 indicated yellow halo zone on NBRIP medium. Significantly high P was solubilized by LGR 16, LGR 15 and LGR 33 In the range of 5.383 – 4.664 mg 100mL-1. Intrinsic antibiotic spectra revealed that Mesorhizobium sp. LGR 4 and LGR 5 showed maximum resistance for different antibiotics (72% and 63%), respectively. Out of 17 Mesorhizobium sp. 88%, 64% and 94% were able to produce siderophore, HCN and ACC, respectively. Salt tolerance ability was reduced with increasing salt concentration, 64% and 58% of the isolates were able to grow at 4% and 5% NaCl concentrations. At low temperatures (5 and 10°C) 15 isolates of mesorhizobia were able to grow on YEMA medium. Two Mesorhizobium sp. LGR 16 (46.3%) and LGR 15 (44.2%) were most effective in controlling Fusarium wilt through production of volatile antifungal compounds in sealed plate technique. All the isolates of Mesorhizobium sp. and reference LGR 33 were evaluated for plant infectivity test with chickpea (GPF 2). Isolates of Mesorhizobium sp. LGR 16 and LGR 15 registered significantly high dry weight of nodules (252.3 and 207.3 mg), N content of nodules (3.36 and 2.95 %) and grain yield (6.3 and 5.8 g) as compared to reference LGR 33 (141.3 mg, 2.34% and 3.6g), respectively. LGR 16 and LGR 15 emerged as effective strains for growth, N2 fixation as well as yield in chickpea. In future these can be explored as potential biofertilizers for extensive testing under field conditions in chickpea.
Research Problem: haracterization of potential Mesorhizobium sp. for functionality traits in chickpea (Cicer arietinum L.)
Issue Date: 2013
Appears in Collections:Thesis

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