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|Advisor:||V. K. Baranwal|
|Title:||Studies on virus(es) associated with grapevine leafroll dise ase in India|
|Abstract:||CHAPTER - I V RESEARCH PAPER - I Serological and polymerase chain reaction (PCR) based d etection of Grapevine leafroll - associated virus 3 (GLRaV - 3) and Grapevine leafroll - associated virus 1 (GLRaV - 1) in the vineyards of India 4.1. Abstract Survey conducted during 2009 - 2010 , 2010 - 2011 and 2011 - 2012 in the vineyards of Jammu and Kashmir, Delhi, Maharashtra and Karnataka states of India showed symptom s typical to leafroll disease of grapevine in the Nashik and Pune region s of Maharashtra and Delhi . DAS - ELISA (double antibody sandwich - enzyme linked immunosorbant assay) and RT - PCR (reverse transcriptase - polymerase chain reaction) based tests showed the presence of Grapevine leafroll - associated virus 3 (GLRaV - 3) in eight cult ivars and Grapevine leafroll - associated virus 1 (GLRaV - 1) in two cultivars of samples collected from the vineyards of Nashik a nd Pune regions of Maharashtra. In a vine of cultivar Shiraj from Nashik region , mixed infection of GLRaV - 3 and GLRaV - 1 was also f ound. However, both the test s for viruses w ere negative in the t wo leafroll symptomatic vines of Delhi . The vineyards of Jammu and Kashmir and Ko ppal district of Karnataka did not exhibit the symptoms of grapevine leafroll disease and serological and PCR b ased assay also confirmed the absence of this disease in these regions. The serology and PCR based detection method s are highly useful in the indexing of grapevine cultivars for the healthy cultivation of grapevine in the Indian viticultural industry. Addi tionally, these tests can also be employed for the quarantine certification programmes of the imported propagating materials.|
|Appears in Collections:||Theses|
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