Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/94069
Authors: THIRUMAL, G
Advisor: Subhash Reddy, R
Title: STUDY OF THE DIFFERENT FACTORS INFLUENCING THE SHELF LIFE OF CARRIER BASED BIOFERTILIZERS
Publisher: PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY. HYDERABAD
Language: en
Type: Thesis
Series/Report no.: D10023;
Abstract: In the present study a total of ten Rhizobial and fifteen Pseudomonas bacterial isolates collected from different sources. These cultures were studied morphologically and biochemically for purity confirmation. All cultures were further screened for their plant growth promoting properties such as P, K, Zn solubilzation, IAA production and biocontrol activity (Siderophore production, HCN production).The screening test results revealed that, among all Rhizobial isolates, GNR-1 showed best plant growth promoting abilities in invitro conditions. Similarly, among all Pseudomonas isolates, RGP-1 had shown better plant growth promoting abilities. These promising cultures were selected for main experiment to evaluate the shelf life of carrier based bioinoculants with different sterilization methods and different carrier materials stored at different temperatures in different intervals. The selected efficient PGP Rhizobium (GNR-1) and Pseudomonas (RGP-1) isolates were multiplied in large quantities in appropriate culture broth by incubating at 28±2°C in an incubator shaker till they attained log phase with a cell load of l×l09cfu ml-1. In order to find the efficient sterilization method (autoclave and irradiation) of carrier materials were followed. For biofertilizer production Vermicompost, Vermiculate, Lignite, and Sodium Alginate carriers were used for bioinoculant preparation and stored at different temperatures i.e. 4ºC and 28ºC. For this experiment, Factorial CRD with treatments of T1:S1C1O1(Autoclaved Vermicompost with Rhizobium spp), T2:S1C1O2(Autoclaved Vermicompost with Pseudomonas spp), T3:S2C1O1(Irradiated Vermicompost with Rhizobium spp), T4: S2C1O2 (Irradiated Vermicompost with Pseudomonas spp), T5:S1C2O1(Autoclaved Sodium alginate with Rhizobium spp), T6:S1C2O2(Autoclaved Sodium alginate with Pseudomonas spp), T7:S2C2O1(Irradiated Sodium alginate with Rhizobium spp),T8:S2C2O2(Irradiated Sodium alginate with Pseudomonas spp), T9:S1C3O1(Autoclaved Lignite with Rhizobium spp, T10:S1C3O2 (Autoclaved Lignite with Pseudomonas spp),T11:S2C3O1(Irradiated Lignite Author : G. THIRUMAL Title of the thesis : STUDY OF THE DIFFERENT FACTORS INFLUENCING THE SHELF LIFE OF CARRIER BASED BIOFERTILIZERS Degree : MASTER OF SCIENCE IN AGRICULTURE Faculty : AGRICULTURE Discipline AGRICULTURAL MICROBIOLOGY AND BIOENERGY Major Advisor : Dr. R. SUBHASH REDDY University PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY Year of submission : 2016 with Rhizobium spp), T12:S2C3O2(Irradiated Lignite with Pseudomonas spp), T13:S1C4O1 (Autoclaved Vermiculite with Rhizobium spp), T14:S1C4O2(Autoclaved Vermiculite with Pseudomonas spp),T15:S2C4O1(Irradiated Vermiculite with Rhizobium spp), T16:S2C4O2 (Irradiated Vermiculite with Pseudomonas spp). The carrier based microbial inoculants prepared with different carrier material was kept in different temperature levels viz., Room temperature (28±2ºC) and Refrigerator (4°C). The surviving populations of PGPB at different temperatures were determined and population was enumerated by dilution plate technique at different intervals i.e monthly upto 8 months. Survival rate of rhizobial and Pseudomonad cells was more upto 8th month at 4ºC compared to 28±2ºC. This results revealed that 4ºC storage temperature is best suitable for storage of carrier based inoculants because of low level of moisture content in the the carrier inoculants stored at 28±20C temperature. Though the bacterial population decreased from 1st month to 8th month the survival rate of rhizobial and Pseudomonad cells was more upto 8th month at 40C. In the view of comparison of both sterilization methods, irradiation sterilized carrier based inoculants showed good survival rate compared to autoclave sterilization method. Similarly with respect to evaluation of shelf life of bacterial cells in different carrier materials like sodium alginate vermicompost lignite and vermiculite, sodium alginate based inoculants maintained good population count upto 8th month. Overall results revealed that sodium alginate based bioinoculants under irradiation sterilization, maintained more viable count upto eights months.
Subject: AGRICULTURAL MICROBIOLOGY
These Type: M.Sc
Issue Date: 2016
Appears in Collections:Theses

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