Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/93740
Authors: D.PRAVEEN KUMAR
Advisor: Dr. L.VENKATESWARA RAO
Title: DETECTION OF ARCOBACTER BUTZLERI IN FOODS OF ANIMAL ORIGIN AND ENVIRONMENTAL SAMPLES IN AND AROUND HYDERABAD
Publisher: PVNR TVU
Type: Thesis
Series/Report no.: D;455
Agrotags: sampling, pcr, livestock, antibiotics, meat, bacteria, biological phenomena, diseases, irrigation, genes
Abstract: The present study was undertaken to optimize PCR assay for detection of Arcobacter spp.and Arcobacter butzleri from foods of animal origin and environmental samples and compare its efficacy with conventional culture methods. Primers derived from 16S rRNA and 23S rRNA genes were used which gave specific amplification products of 181 bp and 686 bp for Arcobacter spp. and Arcobacter butzleri respectively. Out of the 240 samples screened (30 each of chicken, mutton, beef, raw milk, faecal samples of cattle, pig and poultry and water) for Arcobacter spp. PCR gave 31 isolates (chicken meat-6, mutton-4, beef-3, raw milk-2, faecal samples of cattle-4, faecal samples of pig-6 and feacal samples of poultry-5, water-1) as positive and culture method gave 21 isolates (chicken meat-4, mutton-3, beef-2, raw milk-1, faecal samples of cattle-3, faecal samples of pig-4 and feacal samples of poultry-4, water-nil) as positive. Out of 31 positive isolates by PCR, 15 (chicken meat-3, mutton-2, beef-2, raw milk-1, faecal samples of cattle-2, faecal samples of pig-2 and feacal samples of poultry-2, water-1) were positive for Arcobacter butzleri. The Antibiogram test results indicated that Arcobacter butzleri was highly sensitive to azithromycin and gentamicin (100%), followed by streptomycin (95%), ciprofloxacin and tetracycline (90%). Maximum resistance was observed for cephalothin (100%) and vancomycin (90%). Intermediate resistance for the isolates was found to erythromycin (95%) and chloramphenicol (90%) followed by clindamycin (14%), tetracycline and vancomycin (10%).
Subject: Veterinary Public Health and Epidemiology
These Type: M.SC
Issue Date: 2016-03-04
Appears in Collections:Thesis

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