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Advisor: G.C. Gahlot
Publisher: Rajasthan University of Veterinary and Animal Sciences Bikaner-334001 (Rajasthan)
Language: en
Type: Thesis
Agrotags: byproducts, diseases, biological phenomena, livestock, infiltration, animal tissues, proteins, research methods, sampling, dna
Abstract: The present study was undertaken to investigate polymorphism in BMP-15 (Fec XB and Fec XG in exon-2) gene in Marwari goats by RFLP pattern. Samples were collected from 60 unrelated goats of Marwari breed maintained at Daiya, kodemdaser, Deshnok and kalyansar farm of Bikaner district under the goat scheme. Blood samples were collected from Jugular vein of Marwari goats. Genomic DNA was isolated from collected samples by phenol chloroform extraction method. Amplification of BMP- 15 gene was done by Polymerase Chain reaction using corresponding primers having product size of 153 bp and 141 bp for Fec XB and Fec XG gene respectively. DNA isolated from samples revealed proper amplification of exon-2 BMP-15 gene at Fec XB and Fec XG loci. Later on amplicons were purified by phenol extraction method. Restriction digestion of purified amplicons was done using appropriate restriction endonuclease enzyme for studying Restriction fragment length polymorphism in amplified PCR product. All amplified products revealed monomorphism after restriction digestion possessed similar band pattern for exon-2 BMP-15 at Fec XB and Fec XG loci.
Subject: Animal Genetics and Breeding
These Type: M.V.SC
Issue Date: 2010-04-22
Appears in Collections:Thesis

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