Please use this identifier to cite or link to this item:
|Advisor:||Dr. D. MADHURI|
|Title:||TESTICULAR TOXICITY OF DOXORUBICIN IN MALE ALBINO WISTAR RATS AND ITS AMELIORATION WITH QUERCETIN|
|Agrotags:||planting, peas, sowing, yields, developmental stages, crossing over, heterosis, biological phenomena, genetics, land resources|
|Abstract:||Cancer is one of the major life threatening conditions in humans and animals. Due to advances in anticancer chemotherapy, the survival rate of cancer patients have been improved remarkably, but the quality of life (QOL) after successful treatment is still of great concern. Spermatogenetic disorders in male patients are among the major problems caused by chemotherapy. Doxorubicin (DOX) is one of the most potent broad spectrum antitumor anthracycline antibiotic, widely used to treat variety of cancers, including severe leukaemias, lymphomas, and solid tumours. The clinical use of DOX is restricted because of its serious toxicity on various organs viz., heart, liver, lung, kidney, and testis. An experiment was designed to evaluate Doxorubicin induced testicular toxicity and its amelioration with quercetin. The experiment was carried on 48 healthy male albino Wistar rats (weighing 250-280g) according to the guidelines and prior approval of Animal Ethics Committee. Animals were divided into four groups consisting of 12 in each group. The experimental study was designed as follows: Group 1-Control, Group 2-Doxorubicin treated @ 4mg/kg b.wt intraperitoneally weekly once. Group 3-treated with quercetin @ 80mg/kg b.wt, orally daily. Group 4-treated with doxorubicin @ 4 mg/kg b.wt, intraperitoneally weekly once and quercetin @ 80mg/kg b.wt, orally daily. Body weights were recorded weekly once. The animals were sacrificed at fortnight intervals and the blood was collected for estimation of haematological parameters. The testes were collected for tissue biochemical profile, histopathological and ultrastructural studies. The doxorubicin treated group revealed significant (P<0.05) reduction in body weight gain, absolute and relative testicular weight. The epididymal sperm analysis revealed significant (P<0.05) decrease in sperm quantity and quality. Haematological studies revealed that overall mean values of PCV, Hb, TEC and TLC were significantly (P<0.05) reduced in Group 2. Studies on tissue oxidative stress parameters revealed significant (P<0.05) increase of TBARS and significant (P<0.05) decrease of GSH, SOD, CAT and testicular LDH in the Group 2 compared with control. Improvement was noticed in all the above parameters in Group 4 (DOX+QUER) incomparision with Group 2. The histopathological studies of Group 2 showed loss of germ cells with vacuolation in seminiferous tubules and coagulative type of necrosis as well as edema in the interstitial spaces. In later stages severe atrophy of seminiferous tubules with loss of germ cells and giant cells were noticed. In Group 4 the spermatogenesis with several mitotic figures along with mild degenerative changes were noticed. The ultrastructural studies of Group 2 revealed subcellular changes in germ cells like margination of chromatin, swelling of mitochondria and vacuolar changes in cell cytoplasm and the severity was more expressed with complete loss of cells on 29th day. In Group 4 improvement was noticed with appearance of germ cells and sperm cells. From the present study it can be concluded that reproductive toxicity induced by DOX is related to increased oxidative stress. Treatment with quercetin at the dose designed in this study showed moderate improvement in testicular dysfunction. It appears that flavonoid quercetin is a potential antioxidant as an additive to chemotherapeutic drugs that produce testicular toxicity.|
|Appears in Collections:||Thesis|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.