Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/86380
Authors: Sandeep Kumar
Advisor: Yadav, A. S.
Title: Genetic and symbiotic characterization of poly-β-hydroxybutyrate production in Rhizobium and Bradyrhizobium spp. nodulating mungbean [Vigna radiata (L.) Wilczek]
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: Rhizobium/Bradyrhizobium, PHB production, antibiotic resistance symbiotic efficiency
Abstract: Twenty-one WT strains of Rhizobium/Bradyrhizobium sp. (Vigna) were isolated purified and tested for their ability to form nodules on the mungbean plants. These WT strains were screened for PHB production on Beringer’s MM with Nile blue A under UV light. The two WT strains MBR 16 and MBR 25 produced high amount of PHB and these were used to isolate PHB mutants. Three types of mutants were isolated and characterized as MHt, MM and ML mutants. The MHt mutants produced highest amount of PHB, whereas ML mutants produced very low amount of PHB. The Maximum amount of PHB was found in MBR 16 MHt 1 i. e. 1.24 g/l. The antibiotic resistance pattern of parent strains and their mutants was almost same. The symbiotic effectivity of parent strains and their mutants on mugbean plants showed that the MHt mutants had higher shoot dry weight and total shoot nitrogen than the MM and ML mutants. A highly significant positive correlation was found between the amount of PHB produced and shoot dry weight and total shoot nitrogen of the munbean plants.
Issue Date: 2008
Appears in Collections:M. Sc. Dissertations

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