Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/85851
Authors: Trivedi, Abhishek
Advisor: Kharb, Pushpa
Title: In vitro establishment of nitrogen fixing azotobacter chroococcum with brassica juncea L. cv RH-30
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: Sowing, developmental stages, Yields, Irrigation, Wheats, Crops, Grain, Drying, Soaking, Planting
Abstract: Brassica juncea is an amphidiploid with B. nigra (L.) Koch (2n = 16) and B. rapa L. (2n = 20) as parents, It is also known as, Indian mustard or leaf mustard, During 2006-2007 mustard occupied 6.7 million hectares with an annual production of 74 million tonnes in India. B. juncea cultivation is mainly confined to Uttar Pradesh, Rajasthan, Madhya Pradesh, Haryana, Punjab, and Bihar. B.juncea is mainly used in the production of mustard oil. Mustard oil is one of the major edible oils in India. Azotobacter chroococcum is a gram negative, free living nitrogen fixing bacterium and is used as biofertilizer. Several properties of Azotobacter are beneficial for its associated plants like its -IVability to produce various growth regulators and siderophores which results in an overall increase in growth of its associated plants. In the present study, attempts were made to study the establishment of A. chroococcum with calli of B.juncea. Calli of B.juncea were raised using cotyledonary explants. Maximum percent induction of calli from cotyledonary explants was observed in MS medium supplementd with 0.2 mg/l BAP and 0.2 mg/l NAA. To study the establishment of A. chroococcum with callus of B.juncea. Broth of A. chroococcum containing 106 and 107 cfu/ml were used to make 5, 10 and 15ul of A. chroococcum inoculum which were then injected in calli of B.juncea. Calli of B.juncea injected with 10 and 15 μl of bacterial inoculum did not survive because of overgrowth of bacterial cells. Therefore further studies were conducted with 5 μl of bacterial inoculum for injection into calli. The injected calli; after incubation of 4 and 8 days, were surface sterilized and crushed to make a suspension in sterilized water which was plated on Jensen’s nitrogen free medium. Bacterial count was found to increase after 4 and 8 days in calli injected with 5μl on bacterial inoculum, which confirms the associationship A. chroococcum with callus of B.juncea Scanning electron micrograph also confirms the establishment of A. chroococcum with callus of B.juncea.
Issue Date: 2009
Appears in Collections:M. Sc. Dissertations

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