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Authors: Sakthivel, Pavitra
Advisor: Kharb, Pushpa
Title: In vitro regeneration studies in garlic (Allium sativum L.) cvs. HG-17 and G-282
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: Garlic, Regeneration, Vegetative propagation, Planting, Biological phenomena, Cloves, Auxins, Allium sativum, Biological development, Cytokinins
Abstract: Garlic is used worldwide as a spice and a condiment. It has very high nutritive value and is a valuable foreign exchange earner for India. It is well known throughout history for its medicinal properties. It is sexually sterile, hence it is propagated vegetatively. During present investigation, in vitro regeneration in garlic cv. G-282 and HG-17 was studied. Basal part of a vertically cut clove was used as an explant. 2iP was used at 1, 1.5, 3 and 3.5 mg/l while TDZ was used along with 2iP at 0.1, 0.2 and 0.3 mg/l keeping concentration of 2iP constant at 0.1 mg/l. Highest percentage of multiple shoot formation in both G-282 (90.3%) and HG-17 (80.5%) was observed with 3 mg/l 2iP. The average number of shoots produced was highest with MS + 3 mg/l 2iP for both G-282 (8.9 shoots/ explant) and HG-17 (7.5 shoots/explant). IAA (1.5 mg/l) induced maximum percentage of roots for both G-282 (75.0%) and HG-17 (77.4%). Average number of roots was highest in MS + 1.5 mg/l IAA for G-282 (9.9 roots/shoot) and HG-17 (9.5 roots/shoot). 0.5 mg/l NAA + 0.5 mg/l IBA was found to be the ideal concentration for induction of callus using young leaf explants. MS basal medium containing 10% sucrose induced maximum bulblet formation for both G-282 (87.5%) and HG-17 (75.0%). 41.7% of the regenerated plants of the cultivar G-282 and 50.0% of the regenerated plants of the cultivar HG-17 survived under pot conditions.
Issue Date: 2009
Appears in Collections:M. Sc. Dissertations

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