Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/85073
Authors: Pardeep Kumar
Advisor: Dhillon, Santosh
Title: SSR marker analysis of thermotolerant and sensitive genotypes of wheat (Triticum aestivum L. em. Thell)
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: Vegetative propagation, Wheats, Planting, Genotypes, Developmental stages, Drying, Grain, Yields, Acidity, Biological development
Abstract: Hexaploid bread wheat (Triticum aestivum L. em. Thell) is an important cereal food crop for majority of world’s population. SSR markers show high level of polymorphism even in species with narrow genetic base, such as wheat. High-temperature stress is one of the major constrains to wheat production worldwide. This study was undertaken with the objective to assess polymorphism among seven thermosensitive and seven thermotolerant genotypes of wheat. DNA extracted from young leaves of 14 wheat genotypes was amplified by using 45 SSR primers. Out of these primers, 37 showed amplification and were selected for further investigation. For SSR assays, data was analyzed to calculate various parameters such as the number of total bands, number of polymorphic bands, per cent polymorphism, bands per primer, polymorphic bands per primer, similarity matrices and dendrogram construction. The polymorphism percentage ranger from 33.3% to100%, giving an average percentage of polymorphism of 77.8%. The SSR primers yielded average 2.43 bands per primer. Overall size of PCR amplified products ranged between 95bp and 1120bp. Based on SSR similarity matrix data, the value of similarity coefficient ranged from 0.60 to 0.90 with an average genetic similarity of 0.76. At a similarity coefficient 0.751, two group were formed which separated all thermotolerant and thermosensitive genotypes in the dendrogram. Two and three dimensional PCA (Principle Component Analysis) showed similar clustering as evident from cluster tree analysis. Primer Xgwm 46 showed a ≈200bp band which was present in all the thermotolerant genotypes and absent in all thermosensitive genotypes. While WMC 170 produced three unique alleles of 215bp (WH 730 &WH 1021), 217bp (GW 173 & NIAW 34) and 220bp (RAJ 3765 & WH 1076) which were present in all the thermotolerant genotypes (except WH1022) but absent in all the thermosenstive genotypes. Xgwm46 and WMC 170 primers may probably be thermotolerance specific and may have potential for use in marker assisted selection programs for wheat production improvement.
Issue Date: 2010
Appears in Collections:M. Sc. Dissertations

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