Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/81369
Authors: Rajiv Kumar
Advisor: Bimlendra Kumari
Title: Genetic characterization of different clones of poplar (Populus deltoides Marsh.) grown in Haryana
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: Planting, Wheats, Tolerance, Food preservation, Grain, Yields, Genetics, Genotypes, Selection, Genes
Abstract: Growth performance of the 19 promising clones of Populus deltoides, have been statistically analyzed for different growth parameters viz; sprouting percentage, colour and shape of buds (after one month), total height, collar diameter, inter-nodal length, number of roots, number of branches, root length, volume index, total biomass (fresh shoot + fresh root weight ), bark colour and texture, disease resistance (after six months and one year), number of leaves, leaf fall duration, leaf length, leaf width and leaf area index at nursery stage. Taxonomic evaluation (colour and shape of buds) revealed that the clonal characters of the studied represent the source of germplasm from Populus deltoides of section Aigeirous. Results of the studies revealed that clone W39 showed best performance in total height, collar diameter, number of branches, number of roots, volume index and biomass. Clone W32 had more inter-nodal length and root length than others. Clone Udai, G48, W110, S7C8, Bahar, S7C1 were found the most promising clones in this study in all growth parameters. Wimco seedlings ltd. clones showed better performance in all above mentioned growth characters than FRIAM clones. Leaf parameters were statistically analyzed in all 19 clones, these varied significantly . Poplar is a deciduous species, therefore leaf related characters were studied in the winter months while vegetative buds remained stable during whole study. The correlation results indicating the feasibility of selection of clones for higher values of total height, collar diameter, root length and volume index. Leaf samples from the young plants of different clones of poplar were collected for molecular analysis. The extraction method (CTAB), DNA purification, PCR reaction conditions were standardized to obtain genet ic diversity. This study was carried out to find out genetic diversity among the 19 Populus deltoides clones. Out of the 30 RAPD markers used in this study, only 11 showed polymorphic pattern and showed a total of 94 fragments. Out of these, 59 bands were polymorphic and 35 were monomorphic. Average number of fragments amplified was 8.54. Cluster analysis of RAPD data using UPGMA separated 19 clones into 2 major clusters at similarity coefficient at 0.43.The genetic dissimilarity index calculated varied from 0.20 to 0.73 for 19 Poplar clones.The maximum similarity value (0.73) was observed between clone FRIAM 100 and W32 indicating FRIAM 100 and W32 to be most closely related genotypes. In spite of low number of bands that detected polymorphism, RAPD analysis indicated in this study that there is high genetic diversity among Populus deltoides clones analyzed.
Issue Date: 2015
Appears in Collections:M. Sc. Dissertations

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