Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/81325
Authors: Gulati, Susheel
Advisor: Madan, V. K.
Title: Phytochemical studies and antioxidant activity of ashwagandha (Withania somnifera L.)
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: Genotypes, Extraction, Vegetative propagation, Alcohols, Withania somnifera, Aromatic compounds, Antioxidants, Phenols, Sugar, Planting
Abstract: The present study was undertaken for phytochemical studies and antioxidant activity of Ashwagandha (Withania somnifera L.) roots of promising genotypes viz. HWS-08-14, HWS-08-18, HWS-1228, HWS-1229 and Selection-2B & varieties JA-20 and RVA-100 grown at Research Farm of Chaudhary Charan Singh Haryana Agricultural University, Hisar. Chemical parameters viz. crude fibre, minerals (Fe, Cu, Zn, Mn), starch, total sugars, reducing sugars, non-reducing sugars were analysed in ashwagandha roots. Crude fibre ranged from 17.4 to 37.3%, Fe content range d from 595.83 to 983.33 ppm, Cu content ranged from 8.40 to 13.72 ppm, Zn content ranged from 16.33 to 41.00 ppm and Mn content ranged from 16.80 to 33.46 ppm. Starch, total sugars, reducing sugars and non-reducing sugars content ranged from 7.61 to 8.22 mg/g, 5.33 to 6.89 mg/g, 0.40 to 0.64 mg/g and 4.87 to 6.33 mg/g, respectively. Phytochemical parameters viz. total alkaloids, tannins, total phenolics and total flavonoids were also analysed. Total alkaloids and tannins content ranged from 0.26 to 0.31% an d 0.66 to 0.84 mg/g, respectively. Total phenolics and flavonoids were extracted with two solvents namely aqueous and ethanol. Extract yield of aqueous extracts of ashwagandha roots ranged from 7.32 to 13.65 g/100g in comparison to ethanol extracts ranging from 2.66 to 4.84 g/100g showing that aqueous extracts had higher extract yield than ethanol extracts. Total phenolics content (mg GAE/g) in aqueous extracts of ashwagandha roots ranged from 2.41 to 5.37 mg GAE/g d.w.b. and in ethanol extracts ranged from 1.70 to 3.36 mg GAE/g d.w.b. Total flavonoids content (mg CE/g) in aqueous extracts of ashwagandha roots ranged from 0.64 to 1.10 and in ethanol extracts ranged from 0.96 to 1.59 mg CE/g d.w.b. DPPH free radical scavenging activity (%) increased with increase of concentration levels. In aqueous extracts, DPPH free radical scavenging activity of genotype HWS-08-14 was highest ranging from 10.40 to 90.34 (IC 50 1140.5 µg/mL) followed by genotype HWS-08-18 ranging from 6.72 to 87.39 (IC 50 1385.7 µg/mL), genotype HWS-1229 ranging from 7.40 to 76.30 (IC50 1497.8 µg/mL), genotype Selection-2B ranging from 7.23 to 75.49 (IC 50 1856.5 µg/mL), genotype HWS-1228 ranging from 2.09 to 77.64 (IC 50 2463.2 µg/mL) in comparison to varieties JA-20 ranging from 2.82 to 87.32 (IC 50 1578.6 µg/mL) and RVA-100 ranging from 4.47 to 77.27 (IC 50 2615.9 µg/mL) at concentrations levels 100 to 5000 µg/mL. In ethanol extracts, DPPH free radical scavenging activity of genotype HWS-1229 was highest ranging from 5.98 to 72.84 (IC 50 1507.1 µg/mL), genotype HWS-08-14 ranging from 1.98 to 85.11 (IC 50 1533.9 µg/mL), genotype HWS-08-18 ranging from 2.73 to 76.59 (IC 50 1627.9 µg/mL), genotype Selection-2B ranging from 1.54 to 72.80 (IC 50 2632.3 µg/mL) genotype HWS-1228 ranging from 1.40 to 68.01 (IC 50 2648.1 µg/mL) in comparison to varieties JA-20 ranging from 2.48 to 82.27 (IC 50 2384.1 µg/mL) and RVA-100 ranging from 1.42 to 72.22 (IC 50 3307.9 µg/mL) at concentrations levels 100 to 5000 µg/mL. Among aqueous and ethanol extracts, aqueous extracts contained higher amounts of total phenolics and also exhibited higher antioxidant activity. Hence, aqueous extracts of ashwagandha roots are better source of antioxidants in comparison to ethanol extracts.
Issue Date: 2015
Appears in Collections:M. Sc. Dissertations

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