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Authors: John, K.
Chandran, N.D.J.
Ramaswamy, V.
Balachandran, C.
Kirubaharan, J. John
Title: Adaptation of Peste Des Petits Ruminants Vaccine Virus in BHK Monolayer Culture
Publisher: Indian Vet. J.
Language: en
Type: Article
Abstract: The vero adapted PPR vaccine virus was adapted to BHK cell line by fifteen serial passages. The titre of PPR vaccine virus in BHK at 5th, 10th and 15th passage level were 8.23, 8.41 and 8.54 log TCID /0.1 ml respectively. The identification of the PPR vaccine virus adapted in BHK21 cell line was done at 5th, 10th and 15th passage level by cytopathogenicity studies and virus neutralization test. The 5th,10th and 15th passaged PPR vaccine virus was further confirmed with sandwich ELISA.
Issue Date: 2006-11
Appears in Collections:Articles (1.Journal)

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