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Authors: Kumari, Shweta
Advisor: Yadav, R.C.
Title: Morphological and bio chemical characterization of Winged bean Rhizobium and its impact as seed Inoculant either singly or Combinedly with PGPR towards Yield Attributing Characters and Yield of Winged Bean (Psophocarpus tetragonolobus)”
Publisher: Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur
Language: en
Type: Thesis
Pages: 120p. ; plates
Agrotags: null
Keywords: Soil Science, Microbiology, Morphological, Winged bean, PGPR,
Abstract: A laboratory investigation for morphological and bio-chemical characterization and physiological properties of winged bean Rhizobium with a purified culture; and a field experiment consisting of four seed-inoculation treatments [No inoculation, Rhizobium, PGPR(Bacillus) and Rhizobium+PGPR(Bacillus)] and three nitrogen levels [0, 20 and 40 kg ha-1] in RBD (factorial) with three replications for evaluation of two winged bean germplasm IC-17002 and DLN-9, during crop season in the year 2014-15 and with better-performing IC-17002 in 2015-16 to find out impact of the Rhizobium as seed inoculant alone or in combinedly with PGPR on yield attributes and yield of winged bean were conducted at Tirhut College of Agriculture, Dholi Farm of RPCAU, Pusa (Bihar). The pure-culture of winged bean Rhizobium isolate was Gram-negative rod-shaped with a dimension of 0.59 μm x 1.81μm. The Rhizobium tested vis-à-vis (pea, gram, lentil and moong-rhizobia) showed a wide diversity in tolerance for its growth at different pH values, though neutral pH being the optimum. The winged bean-Rhizobium isolate produced 64.6 μg mL-1 of IAA at 72h of incubation with antibiotic-resistance and carbon-utilization test, the Rhizobium isolate was found to be resistant to vancomycin (30μg) and utilized a wider group of carbon sources than other rhizobia. The Fatty-Acid-Methyl-Ester (FAME) profiling confirmed that it belonged to Rhizobium-miscellany group. Seed inoculation and nitrogen levels greatly influenced nodule-parameters, nitrogenase activity and yield attributes, value of which were the highest with the combined inoculation of Rhizobium+PGPR (Bacillus), followed by seed inoculation of Rhizobium alone, and differed significantly among themselves at both 60 and 120 days after sowing (DAS). Nitrogen application @ 20 kg ha-1 recorded significantly higher nodule parameters over the control. The inoculation x nitrogen interaction effect was found to be significant with respect to nodule parameters. The combined inoculation of Rhizobium+PGPR (Bacillus) at 20 kg N ha-1 appeared significantly superior to other treatment combinations. S, Fe and Mo in the nodule recorded higher at 60 DAS than at 120 DAS. Rhizobium inoculation alone caused higher S and Mo content in nodule, whereas combined application of Rhizobium and PGPR recorded the highest Fe content at both growth stages. Nitrogen application @ 20 kg ha-1 increased Fe content significantly over no-nitrogen application at 120 DAS. The highest nitrogen content in pod was noted by combined inoculation of Rhizobium and PGPR treatment, followed by Rhizobium alone. Nitrogen @ 20 kg ha-1 was found to be superior to the higher level of N application. The combined inoculation of Rhizobium and PGPR at 20 kg N ha-1 proved better than the other treatments. The treatments recorded greater N and P content of winged bean plant at 60, 120 DAS and at harvest. In seed, Rhizobium and PGPR inoculation in combination recorded more N content than other treatments and was found to be at par with Rhizobium inoculation alone. Nitrogen application @ 20 kg ha-1 recorded higher N, P and K in seed of winged bean in first year only. Protein content in green edible pod, seed and tuber was significantly increased by combined inoculation of Rhizobium and PGPR over the control and was at par with the treatment of Rhizobium inoculation alone, whereas basal nitrogen @ 20 kg ha-1 favoured more protein content than higher level of nitrogen. After harvest of winged bean, the highest available soilnitrogen build-up was recorded with the treatment of Rhizobium inoculation alone. Seed-oil content (33.76 %) recorded the highest in the treatment with seed inoculation of PGPR at zero nitrogen application. The study proved the multiple-utilitarian potential of winged bean including its role in soil fertility improvement which was best with seedinoculation of Rhizobium-miscellany and PGPR either alone or in combination with basal dose of 20 kg N ha-1.
Subject: Soil Sciences
Theme: Morphological and bio chemical characterization of Winged bean Rhizobium and its impact as seed Inoculant either singly or Combinedly with PGPR towards
These Type: Ph.D
Issue Date: 2018
Appears in Collections:Thesis

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