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Authors: Kamboj, Aarti
Advisor: Kharb, Pushpa
Title: Genetic diversity analysis in Kiwifruit (Actinidia spp.) using RAPD/SSR markers
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: null
Keywords: Kiwifruit, Genetic Diversity, RAPD markers
Abstract: Kiwifruit (Actinidia spp.) is a very important plantation crop, generally dioecious, deciduous and scrambling vines with chromosome number 2n=58, belonging to family Actinidiaceae. Dioecy in kiwifruit lays an important constraint for breeding programs. The present study was undertaken to study genetic diversity in kiwifruit genotypes using RAPD markers. Molecular polymorphism in 7 kiwifruit genotypes (2 males viz. Tomuri & Allision and 5 females viz. Bruno, Hayward, Monty, Allision & Abbott) was evaluated using 94 RAPD primers, out of which 23 were found to amplify the genomic DNA in all the seven genotypes. Analysis of RAPD data was done using NTSYS-pc software and dendrogram was constructed using UPGMA method. The 7 kiwifruit genotypes grouped in two main clusters comprising of male and female genotypes separately. Similarity matrix indices ranged from 0.521 to 0.719 showing maximum similarity between Allision (M) and Tomuri (M) with a similarity coefficient of 0.719 while Allision (M) and Abbott (F) were found least similar with a similarity coefficient of 0.521. Primers OPA-01, OPB-02, OPB-20 and OPC-05 amplified unique amplicons in Bruno, Hayward, Abbott, Monty and Allision (M), and primers OPA-02 & OPN-02 amplified unique amplicons in Tomuri (M) & Allision (M) and male and female plants of Allision genotype respectively. Thus, these primers can be used to distinguish the kiwifruit genotypes and can also be validated as putative sex linked markers which have the prospective to be used for further characterization in specific kiwifruit genotypes.
Subject: Biotechnology and molecular Biology
Theme: Genetic diversity analysis in Kiwifruit (Actinidia spp.) using RAPD/SSR markers
These Type: M.Sc
Issue Date: 2019
Appears in Collections:Theses

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