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|Title:||Evaluation of in vitro cytotoxicity and anti-diabetic property of Aloe spp. based on over-expression of glucose transporter (Glut-4)|
|Publisher:||G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)|
|Keywords:||in vitro, cytotoxicity, diabetes, Aloe, glucose, transporter|
|Abstract:||The world wide epidemic of diabetes has stimulated the search of new concepts, formulations and the targets for the treatment of this disease. As existing conventional treatments for diabetes have several side effects and also they are not able to nullify diabetic complications completely. Aloe spp. claims as diabetes therapeutics has been investigated in vivo by several workers. Their results concluded that Aloe extracts possess anti-diabetic property. Their mode of action and molecular targets are yet to be determined. The objective of the present study was to analyze the influence of Aloe extracts (aqueous and ethanolic) on the cytotoxicity and to evaluate its effect on GLUT-4 gene expression on NIH/3T3 cell line as in vitro model for screening the anti-diabetic properties of plants. Under present investigation, attempts were made to investigate possible compositional differences among Aloe spp, dose-dependent cytotoxicity of Aloe extracts for determining the optimum dosage for in vitro assay and its effect on GLUT-4 gene expression. The in vitro cell culture system developed can be suitably employed for screening of anti-diabetic properties of several herbal formulations and plants. Spectral analysis of Aloe extracts indicated the presence of diverse composition and compositional variations between two Aloe species (Aloe vera & Aloe perryi). Aloe extract showed cytotoxic effects on cell in a dosedependent manner, influenced the doubling time and saturation density of NIH/3T3 cells as shown by growth kinetics in presence and absence of Aloe extracts. The cell‟s viability was also affected in a dose-dependent manner. After determination of optimal dose for in vitro study, GLUT-4 gene expression study was performed at transcript level by using semiquantitative RT-PCR with mouse GLUT-4 specific primers. Under defined culture-conditions and standardization in our Lab, Aloe extracts appear to up regulate the GLUT-4 mRNA expression as compared with untreated cells. Insulin and Metformin was used as standard references. Water soluble Aloe extracts (1 mg/ml) stimulated the GLUT-4 gene expression with their optimal effect at 36 hours of treatment. GLUT-4 expression appears to increase gradually and attained a steady-state after 36 hours of treatment and thereafter a decline in GLUT-4 expression was observed indicating the temporal down regulation perhaps due to gradual increase in apoptotic signal at later stages. Our data suggest that Aloe extract possesses the anti-diabetic activity which might be utilized for drug formulations if suitable molecules get identified. Results also explain why Aloe plants have been used in some traditional medicines to ameliorate the diabetic symptoms.|
|Theme:||Molecular Biology and Biotechnology|
|Appears in Collections:||Theses|
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