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Authors: Raypa, Pratima
Advisor: Dubey, Ashutosh
Title: Studies on micropropagation with metabolite profiling for pharmacogenosic efficacy of Adina cordifolia and biochemical investigations of flowering in Dendrocalamus giganteus
Publisher: G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Language: en
Type: Thesis
Pages: 234
Agrotags: null
Keywords: micropropagation, metabolites, profiling, pharmacognosy, Adina cordifolia, biochemistry, flowering, Dendrocalamus giganteus
Abstract: Adina cordifolia a Rubiaceae family member is deciduous threatened tree and for in vitro propagation, axillary buds were augmented on MS medium supplemented with naphthalene acetic acid and 6- benzyl amino purine. Shoot length of propagated explants were found maximum on medium supplemented with 2 mg/L BAP or 0.5 mg/L NAA while maximum callus volume was induced in MS medium supplemented with 1.0 mg/L NAA, 1.0 mg/ L 2, 4- D and 0.5 mg/L BAP. On the metabolite profiling of Adina cordifolia different extracts through GC-MS, dominating constituents in the leaf extracts were trans squalene, vitamin E, phytol, and neophytadiene, while in bark extracts 7-hydroxycoumarin, nhexadecanoic acid, methyl commate, ergost-5-en-3.beta.-ol, -sitosterol. In root extracts major compounds, acetophenone, benzoic acid, 7-hydroxycoumarin, n-hexadecanoic acid, ergost-5-en-3.beta.-ol, beta.-sitosterol and gamma.-sitosterol were found. Through HPLC 7-hydroxycoumarin (7-HC), pharmaceutically important compound was found in high quantity in methonolic extracts of root, bark and calluses. Reducing power activity was positively correlated with all phytochemicals but best correlation was with phenols and flavonoids. FRAP activity were found positively correlated with phenolic content and flavonoid. Adina cordifolia extracts showed good cytotoxicity against human pathogenic strains i.e., Salmonella typhaemurium 52, Salmonella typhaemurium 149, Salmonella typhaemurium ATCC 51812, Salmonella entiritidis ATCC 13076, E. coli ATCC-25922 and E coli strain-13. Allelopathic effect of leaf extract of Adina cordifolia was studied on wheat genotypes as H2O2 content increment in wheat genotypes due to salt stress. Aqueous leaf extract of Adina cordifolia treatments decreased H2O2 content with increase in proline content. Flowering gene expression analysis in bamboo genotype Dendrocalamus giganteus was carried out by RT-PCR analysis. The transcript accumulation of homolog of CRABS CLAW, SEPALLATA1, AGAMOUS and PISTILLATA in flowering bud of Dendrocalamus giganteus was 3.6, 1.41, 3.02 and 5.46 folds higher respectively as compared to non-flowering bamboo leaves of Dendrocalamus giganteus. Metabolite profiling of D. giganteus revealed the presence of dominating constituents such as -thujene, vitamin E, 6-methyl-2-(5-methyl-5-vinyltetrahydro-2-furanyl)-5-hepten-3-one, elemol, -eudesmol, 7-epi- -eudesmol, n-hexadecanoic acid, n-heneicosane, n-tetracontane, campesterol, -sitosterol. Thus these results on A. cordifolia would be used to set new standard of prescribing the dosage of herbal drugs to propose an alternative to modern system of medicines and bamboo flowering gene expression analysis provide good understanding of flowering gene expression pattern.
Subject: Biochemistry
Theme: Molecular Biology and Biotechnology
Research Problem: Dendrocalamus giganteus
These Type: Ph.D
Issue Date: 2019-02
Appears in Collections:Theses

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