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Authors: Abirami, S
Sangaran, A
Sreekumar, C
Porteen, K
Title: Evaluation of Cysticercus cellulosae antigens for the diagnosis of cysticercosis in pigs by Dot–ELISA
Other Titles: Journal of Veterinary Parasitology
Publisher: TANUVAS
Language: en
Type: Article
Pages: 42-44
Volume: 32
Series/Report no.: ;1
Agrotags: Veterinary Science
Keywords: Pigs, Cysticercosis, Immunoassay, Excretory-secretory antigens, Dot-ELISA.
Abstract: An immunoassay (Dot–ELISA) for diagnosis of cysticercosis in pigs using the excretory-secretory antigen (ES) and somatic antigens of Cysticercus cellulosae was standardized. Antigens were prepared from the cysts isolated from a spontaneous case of severe cysticercosis in a desi pig. Blood samples were collected from pigs slaughtered at local slaughter houses and organized and unorganized pig farms in and around Chennai. A total of 175 pig sera were screened for antibodies against C. cellulosae. Dot- ELISA was standardized using both antigens of C. cellulosae by checker board titration method. The results indicated 46 (26.28%) positive cases with E/S antigen whereas somatic antigen could detect 29 (16.57%) samples as positive. The immunoassay, using the ES antigen, can present a rapid and inexpensive alternative for the diagnosis of cysticercosis due to C. cellulosae in pigs and possibly humans.
Description: TNV_JVP_2018_32(1)42-44
Issue Date: 2018
Appears in Collections:Articles (1.Journal)

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