Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810131290
Authors: Cauveri, D
Sivaselvam, SN
TANUVAS
Title: NUCLEOTIDE VARIATIONS IN LEPTIN (LEP) GENE – A COMPARISON BETWEEN THE NILAGIRI, MERINO AND DORSET x NILAGIRI SHEEP
Other Titles: International Journal of Science, Environment and Technology
Publisher: TANUVAS
Language: en
Type: Article
Pages: 591-594
Volume: 8
Series/Report no.: ;3
Agrotags: Veterinary Science
Keywords: Sheep, Leptin gene, indigenous breeds, Merino, SNPs.
Abstract: LEP gene is one of the potential genes that is involved intricately in the metabolism and growth of animals. The characterization of LEP gene as compared with the exotic breeds would help us understand the genetic diversity of indigenous breeds. There were five variations between the indigenous (Nilagiri), exotic (Merino) and one crossbred (Dorset x Nilagiri) sheep. In Intron 1 at 13774 bp a ‘GTT’ segment which was present in two copies in the reference sequence was found as a single copy in Nilagiri. In the intronic regions that were amplified along with Exon 2, the transitions 13893 T>C (SNP-L1) in Intron 1, 14074 A>G (SNP-L2) and 14090 G>A (SNP-L3) in Intron 2 were identified. For SNP-L1, Nilagiri and Dorset x Nilagiri had only CC genotypes while Merino had both T and C alleles. The SNP-L3 was found in Merino and Dorset x Nilagiri breeds while Nilagiri breed did not vary from the reference sequence. Analysis of the sequenced regions from the amplified region of Exon 3 revealed one variation 16973 G>A (SNP-L4) which was fixed with A allele in all the breeds studied here as compared to G in the reference sequence.
Description: TNV_IJSET_2019_8(3)591-594
Issue Date: 2019-06
Appears in Collections:Articles (1.Journal)

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