Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810131157
Authors: Susmita Bera
Advisor: Dr. M. PADMA
Title: GENETIC DIVERSITY, VARIABILITY, CORRELATION AND RAPD BASED PROFILING OF GENOTYPES IN SENNA (Cassia angustifolia)
Publisher: College of Horticulture, Rajendranagar, HYD-30
Citation: Sri Konda Laxman Telangana State Horticultural University, Rajendranagar, Hyderabad-30
Language: en
Type: Thesis
Pages: 182
Agrotags: null
Keywords: GENETIC DIVERSITY, VARIABILITY, CORRELATION AND RAPD BASED PROFILING OF GENOTYPES
Abstract: The present investigation entitled “Genetic diversity, variability, correlation and RAPD based profiling of genotypes in senna (Cassia angustifolia)” was undertaken to estimate the genetic divergence and yield component analysis through correlation, path analysis. Twenty two genotypes were sown in a Randomized Block Design with three replications to evaluate genetic divergence. Single row containing 7 plants of each genotype were sown during Kharif, 2018 at Medicinal and Aromatic Plants Research Station, Sri Konda Laxman Telangana State Horticultural University, Rajendranagar, Hyderabad. The result of mean performance of twenty two genotypes for twenty seven characters revealed that the genotype, ACC-4 was the earliest to show germination (6.24 days), but DCA-3 reached 50 per cent germination earliest among other genotypes (8.44 days) though its seeds started to germinate a bit late. Yet, again, ACC-4 recorded highest germination percentage mean (98.7 %). The highest means for plant height (103.17 cm) was recorded by DCA-4; number of pods per plant By DCA-6 (534.25), fresh pod weight and harvest index(0.91g , 39.79% respectively) by DCA-8, dry pod weight (0.35g) by DCA19 ; protein content by DCA-3(18.43%); fibre content by DCA-2(12.4%); sennosoide content by Sona(3.50%). The genotype DCA-15 recorded the highest mean value of 28.94 for number of primary branches per plant. The genotype ACC-4 was early to first flowering (46.7 days) and similarly for 50 per cent flowering (47.4 days); days to first fruit maturity (70.33 days); days to maturity (85.59 days) days to last harvest (60.70 days) and crop duration (103.03 days), the genotype DCA-9(42.67 days) was early to first harvesting. The highest number of flowers per axil (15.44) were borned by the genotype DCA-13. DCA-6 recorded the highest mean value 4.74 cm for pod length. The genotype, DCA-7 recorded the highest mean value of 1.83 cm for pod width.DCA-18 recorded with 7.62 number of seeds per pod, which was the highest genotypes. Test seed weight was found to be heaviest in DCA-8 as depicted by the mean value of 2.96 g. And finally, the highest leaf and pod yield per plant was obtained in the genotypes DCA-20 and DCA-15 (0.27 and 1.78 kg, respectively.) Mahalanobis’ D² analysis established the presence of wide genetic diversity between 22 genotypes for 12 important characters by the formation of nine clusters. The intra cluster generalized distance ranged from 882.32 in cluster I to 3233.81 in cluster VI. High inter cluster distance was observed between cluster VIII and IX (107998.20) followed by cluster VII and IX (96000.95). Heritability values were high for all the characters studied except for pod width. High heritability coupled with high genetic advance as per cent of mean was recorded for the characters viz., days taken for initiation of germination, days taken for 50 per cent germination, germination %, plant height, number of primary branches per plant, days to first flowering, days to 50 per cent flowering, number of flowers per axil, days to pod set, days to first fruit maturity, days to first harvest, days to last harvest, duration of the crop, pod length, number of pods per plant, days to maturity, fresh pod weight, dry pod weight, test weight of seeds, leaf yield/plant, pods yield/plant, harvest index, protein content, fibre content and sennosoide content. From correlation studies, yield per plant exhibited non-significant positive association with the important traits. Direct selection based on these traits could result in simultaneous improvement of traits and leaves and pods yield in senna. In the path analysis, the characters viz. initiation of germination, days to 50 per cent flowering, number of flowers per axil, days to pod set, days to first harvest , days to last harvest, days to maturity, fresh pod weight, number of seeds per pod showed positive correlation and positive direct effect on yield per plant. RAPD based profiling of 22 genotypes, was carried out with 20 primers. Of which 15 amplified with the genotypes and 5 failed to amplify. The dendrogram generated by UPGMA based on Jaccard's similarity coefficients generated 4 clusters at 49% similarity revealing good amount of variability among the genotypes. The PIC values ranged from 0.150 to 0.875 which shows high variability among the genotypes. Based on the genetic studies, the genotypes such as DCA-7, DCA-11, DCA-12, DCA-17 and DCA-19 have been identified as promising genotypes with particular reference to the characters viz., plant height, fruit length, fruit width, average fresh and dry weight, leaf and pod yield per plant and sennosoide content. Sona variety, considered as check, has highest sennosoide content and hence can be used as parent for the purpose of increasing quality of other genotypes in breeding pogrammes. For earliness in flowering, maturity and harvest ACC-4 and ACC-1 can be utilised effectively in breeding and crossing pogrammes. If seed production is a concern then DCA-18 produces more number of seeds per pod with moderate average seed weight. Hence, in conclusion the high range of variability among genotypes can be tapped through breeding pogrammes and potential varieties can be released by following procedure in vogue for the welfare of our farmers.
Description: Sona variety, considered as check, has highest sennosoide content and hence can be used as parent for the purpose of increasing quality of other genotypes in breeding pogrammes. For earliness in flowering, maturity and harvest ACC-4 and ACC-1 can be utilised effectively in breeding and crossing pogrammes. If seed production is a concern then DCA-18 produces more number of seeds per pod with moderate average seed weight. Hence, in conclusion the high range of variability among genotypes can be tapped through breeding pogrammes and potential varieties can be released by following procedure in vogue for the welfare of our farmers.
Subject: Spices, Plantation and Medicinal and Aromatic Plants
Theme: Sona variety, considered as check, has highest sennosoide content and hence can be used as parent for the purpose of increasing quality of other genotypes in breeding pogrammes. For earliness in flowering, maturity and harvest ACC-4 and ACC-1 can be utilised effectively in breeding and crossing pogrammes. If seed production is a concern then DCA-18 produces more number of seeds per pod with moderate average seed weight. Hence, in conclusion the high range of variability among genotypes can be tapped through breeding pogrammes and potential varieties can be released by following procedure in vogue for the welfare of our farmers.
Research Problem: GENETIC DIVERSITY, VARIABILITY, CORRELATION AND RAPD BASED PROFILING OF GENOTYPES IN SENNA (Cassia angustifolia)
These Type: M.Sc
Issue Date: 2019-07-28
Appears in Collections:Thesis

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