Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810108188
Authors: PRASANTH K.
Advisor: B. Varalakshmi
Title: GENETIC ANALYSIS AND IDENTIFICATION OF MOLECULAR MARKERS LINKED TO POWDERY MILDEW RESISTANCE IN BITTER GOURD (MOMORDICA CHARANTIA L.)
Publisher: division of horticulture ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI –
Language: en_US
Type: Thesis
Agrotags: null
Abstract: Bitter gourd (Momordica charantia L.) is one of the widely grown cucurbitaceous vegetable crops in India. However, the production is limited by various diseases of which powdery mildew is a major fungal disease in most of the production areas. An experiment was designed to identify resistant source for powdery mildew, to study the inheritance pattern of resistance and to identify a molecular marker linked to powdery mildew resistance gene. The experiments were carried out in the Vegetable Research Block VIII, Division of Vegetable Crops, ICAR- Indian Institute of Horticultural Research, Hessaraghatta, Bengaluru, Karnataka, during 2015 to 2018. Thirty one genotypes were screened under natural field conditions and artificial epiphytotic conditions to identify stable resistant source for powdery mildew. Two genotypes viz., IIHR-80-1-2 and IIHR-80-1-3 were immune to the disease, whereas, IIHR-144-1 was highly resistant reaction to powdery mildew with PDI of 1.81 and 4.28 under natural and artificial screening, respectively. IIHR-40-1 and IIHR-49-34-5 were moderately susceptible with a PDI of 31.1 and 34.6 respectively. Rest of the genotypes showed high susceptibility to powdery mildew. The six generation analysis (P1, P2, F1, F2, B1 and B2) of two cross combinations (Arka Harit × IIHR-144-1 and IIHR Sel-5-8 × IIHR-144-1) indicated that resistance in both crosses were controlled by interaction of two pairs of dominant and recessive genes (dominant inhibitory epistasis, 13:3). Quantitative analysis of resistance to powdery mildew in bitter gourd confirmed the insufficiency of simple additive - dominance model. The epistatic interaction was of greater magnitude than main gene effects in both the crosses and dominance × dominance (l) effects were higher magnitude and of duplicate nature. The estimates of gene effects of fruit colour and pattern of ridge indicated that green fruit colour and discontinuous ridges in bitter gourd was governed by single dominant gene. A total of 300 SSR markers were employed to identify polymorphic primers using 193 F2 populations of Arka Harit × IIHR-144-1 to identify linked marker for powdery mildew resistance through BSA. The primer McSSR 57 produced a polymorphic DNA fragment that co-segregated with disease reaction. PCR amplification of all the 193 plants of F2 population using McSSR 57 gave a recombinant frequency of 18.13 % which indicates that the marker is linked to the gene for resistance at 18.13 cM distance. Considering the cross compatibility between Momordica charantia L. and M. charantia var. muricata (IIHR-144-1) the resistance trait can be easily transferred to cultivated species through hybridization and selection. 110 Since epistasis is of duplicate nature, selection has to be delayed until later generations. Marker assisted selection (MAS) can be employed for early generation selection of powdery mildew resistance gene in bitter gourd and its introgression to develop a resistant variety or hybrid in the desired genetic background. Key words: bitter gourd; epistasis; genetics; molecular marker; powdery mildew
Description: t-10020
Subject: Horticulture
Theme: GENETIC ANALYSIS AND IDENTIFICATION OF MOLECULAR MARKERS LINKED TO POWDERY MILDEW RESISTANCE IN BITTER GOURD (MOMORDICA CHARANTIA L.)
These Type: Ph.D
Issue Date: 2019
Appears in Collections:Theses

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